ABSTRACT
In this review we consider the role of the piRNA system in transposable element silencing in the Drosophila melanogaster germline. We focus on new data that demonstrate the mechanisms of initiation of piRNA biogenesis in ovarian germinal cells and the role of Piwi protein in this process, including our own results.
Subject(s)
DNA Transposable Elements/physiology , Gene Silencing/physiology , Ovary/metabolism , Ovum/metabolism , Animals , Drosophila melanogaster , FemaleABSTRACT
The genome expression pattern is strongly modified during the heat shock response (HSR) to form an adaptive state. This may be partly achieved by modulating microRNA levels that control the expression of a great number of genes that are embedded within the gene circuitry. Here, we investigated the cross-talk between two highly conserved and universal house-keeping systems, the HSR and microRNA machinery, in Drosophila melanogaster We demonstrated that pronounced interstrain differences in the microRNA levels are alleviated after heat shock (HS) to form a uniform microRNA pattern. However, individual strains exhibit different patterns of microRNA expression during the course of recovery. Importantly, HS-regulated microRNAs may target functionally similar HS-responsive genes involved in the HSR. Despite the observed general downregulation of primary microRNA precursor expression as well as core microRNA pathway genes after HS, the levels of many mature microRNAs are upregulated. This indicates that the regulation of miRNA expression after HS occurs at transcriptional and post-transcriptional levels. It was also shown that deletion of all hsp70 genes had no significant effect on microRNA biogenesis but might influence the dynamics of microRNA expression during the HSR.
Subject(s)
Drosophila melanogaster/genetics , Heat-Shock Response , High-Throughput Nucleotide Sequencing/methods , MicroRNAs/genetics , Sequence Analysis, RNA/methods , Animals , Drosophila Proteins , Drosophila melanogaster/classification , Gene Deletion , Gene Expression Regulation , HSP70 Heat-Shock Proteins/genetics , RNA InterferenceABSTRACT
Different types of stress including heat shock may induce genomic instability, due to the derepression and amplification of mobile elements (MEs). It remains unclear, however, whether piRNA-machinery regulating ME expression functions normally under stressful conditions. The aim of this study was to explore the features of piRNA expression after heat shock (HS) exposure in Drosophila melanogaster. We also evaluated functioning of piRNA-machinery in the absence of major stress protein Hsp70 in this species. We analyzed the deep sequence data of piRNA expression after HS treatment and demonstrated that it modulates the expression of certain double-stranded germinal piRNA-clusters. Notable, we demonstrated significant changes in piRNA levels targeting a group of MEs after HS only in the strain containing normal set of hsp70 genes. Surprisingly, we failed to detect any correlation between the levels of piRNAs and the transcription of complementary MEs in the studied strains. We propose that modulation of certain piRNA-clusters expression upon HS exposure in D. melanogaster occurs due to HS-induced altering of chromatin state at certain chromosome regions.
ABSTRACT
The development of the reproductive organs and the gametogenesis of animals are complex and multistage processes that require the precise and effective regulation. In this review, we summarized the recent findings about essential functions of miRNAs in the regulation of gene expression during the differentiation of germ cells in spermatogenesis and oogenesis. Most probably, the main functions of the conservative and highly-expressed miRNAs in the reproductive organs of mammalian are the control of differentiation and proliferation of cells. Also we discussed the possible involvement of the germline-expressed miRNAs in the formation of the reproductive barriers and speciation.
Subject(s)
Gametogenesis , Gonads/metabolism , MicroRNAs/metabolism , Animals , Gene Expression Regulation, Developmental , Gonads/physiology , MicroRNAs/geneticsABSTRACT
This review focuses on the biological role of one class of plant small RNAs, ~22-nt microRNAs (miRNAs). The majority of plant miRNA targets are genes encoding the effector factors of cell signaling pathways. The regulation of their expression is necessary for both ontogenesis and rapid response of plants to biotic and abiotic stress factors. We also summarized current views on the biogenesis and evolution of plant miRNAs as well as the techniques used for their investigation.
Subject(s)
MicroRNAs/metabolism , Plants/genetics , Biological Evolution , Computational Biology , Gene Expression Regulation, Plant , Nucleic Acid Hybridization , Oligonucleotide Array Sequence Analysis , Stress, PhysiologicalABSTRACT
Disturbances of microRNA generation and functioning as inhibitors of gene expression at the translational level are considered as specific and diagnostic features of cancer. This review also highlights the role of short interfering RNA (siRNA) in modified epigenomic chromatin structure, which may cause cancer transformation. Future directions of cancer epigenomics are considered in the light of the involvement of siRNA in epigenomic modification of chromatin.
