Michelson Interferometer for Global High-Resolution Thermospheric Imaging (MIGHTI) On-Orbit Wind Observations: Data Analysis and Instrument Performance.

The design, principles of operation, calibration, and data analysis approaches of the Michelson Interferometer for Global High-resolution Thermospheric Imaging (MIGHTI) on the NASA Ionospheric Connection (ICON) satellite have been documented prior to the ICON launch. Here we update and expand on the MIGHTI wind data analysis and discuss the on-orbit instrument performance. In particular, we show typical raw data and we describe key processing steps, including the correction of a "signal-intensity dependent phase shift," which is necessitated by unexpected detector behavior. We describe a new zero-wind calibration approach that is preferred over the originally planned approach due to its higher precision. Similar to the original approach, the new approach is independent of any a priori data. A detailed update on the wind uncertainties is provided and compared to the mission requirements, showing that MIGHTI has met the ICON mission requirements. While MIGHTI observations are not required to produce absolute airglow brightness profiles, we describe a relative brightness profile product, which is included in the published data. We briefly review the spatial resolution of the MIGHTI wind data in addition to the data coverage and data gaps that occurred during the nominal mission. Finally, we include comparisons of the MIGHTI wind data with ground-based Fabry-Perot interferometer observations and meteor radar observations, updating previous studies with more recent data, again showing good agreement. The data processing steps covered in this work and all the derived wind data correspond to the MIGHTI data release Version 5 (v05).

Evaluation of Reliability and Validity of Occlusal Caries Detection by Direct Visual, Indirect Visual and Fluorescence Camera Using ICDAS II (Codes 0, 1, and 2): An In Vivo Study.

Introduction: Early detection of carious lesions paves the way for the preservation of tooth structures by remineralization strategies. Visual examination using international caries detection and assessment system (ICDAS) II has been validated with the gold standard of histological classification. A fluorescence camera is yet another diagnostic aid for the early detection of carious lesions based on the autofluorescence of the enamel and dentin. This study was done to evaluate the validity of the fluorescence camera (Soprocare and Acteone) in detecting early carious lesions. Materials and methods: A total of 690 occlusal surfaces of non-cavitated premolars and molars were examined by examiners 1 and 2, trained in the ICDAS II grading system. The photographs of the occlusal surface and the fluorescence images, which were taken using a Soprocare (Acetone) intraoral camera, were examined by examiners 3 and 4. The scoring was tabulated and correlated. Results: The κ values for interexaminer reproducibility of indirect visual was 0.841 (good), and his fluorescence camera was 1.00, which is very good. The correlation analysis revealed that there was a higher correlation between direct visual and indirect visual for both examiners when compared to direct and fluorescence cameras. There was a positive relationship between indirect visual and fluorescence cameras for both the experimenter). The indirect visual method for the detection of carries has high sensitivity and specificity irrespective of the examiner. Examination by fluorescence camera has a low sensitivity and high specificity. Conclusion: The specificity of the caries detection method by indirect visual examination based on ICDAS II coding that of the Soprocare fluorescence camera was consistent and reliable, whereas indirect visual examination had a high sensitivity for detecting ICDAS codes 1 and 2. Soprocare showed a very low sensitivity in detecting ICDAS code 1 and 2 lesions. How to cite this article: Dhanavel C, Sai CK, Neelamurthy PS, et al. Evaluation of Reliability and Validity of Occlusal Caries Detection by Direct Visual, Indirect Visual and Fluorescence Camera Using ICDAS II (Codes 0, 1, and 2): An In Vivo Study. Int J Clin Pediatr Dent 2023;16(1):74-78.

Tamil Nadu Pregnancy and Heart Disease Registry (TNPHDR): design and methodology.

BACKGROUND: Cardiac disease in pregnancy is a major contributor to maternal mortality in high, middle and low-income countries. Availability of data on outcomes of pregnancy in women with heart disease is important for planning resources to reduce maternal mortality. Prospective data on outcomes and risk predictors of mortality in pregnant women with heart disease (PWWHD) from low- and middle-income countries are scarce. METHODS: The Tamil Nadu Pregnancy and Heart Disease Registry (TNPHDR) is a prospective, multicentric and multidisciplinary registry of PWWHD from 29 participating sites including both public and private sectors, across the state of Tamil Nadu in India. The TNPHDR is aimed to provide data on incidence of maternal and fetal outcomes, adverse outcome predictors, applicability of the modified World Health Organization (mWHO) classification of maternal cardiovascular risk and the International risk scoring systems (ZAHARA and CARPREG I & II) in Indian population and identify possible gaps in the existing management of PWWHD. Pregnancy and heart teams will be formed in all participating sites. Baseline demographic, clinical, laboratory and imaging parameters, data on counselling received, antenatal triage and management, peripartum management and postpartum care will be collected from 2500 eligible participants as part of the TNPHDR. Participants will be followed up at one, three and six-months after delivery/termination of pregnancy to document study outcomes. Predictors of maternal and foetal outcome will be identified. DISCUSSION: The TNPHDR will be the first representative registry from low- and middle-income countries aimed at providing crucial information on pregnancy outcomes and risk predictors in PWWHD. The results of TNPHDR could help to formulate steps for improved care and to generate a customised and practical guideline for managing pregnancy in women with heart disease in limited resource settings. TRIAL REGISTRATION: The TNPHDR is registered under Clinical Trials Registry-India ( CTRI/2020/01/022736 ).

Validation of ICON-MIGHTI Thermospheric Wind Observations: 2. Green-Line Comparisons to Specular Meteor Radars.

We compare coincident thermospheric neutral wind observations made by the Michelson Interferometer for Global High-Resolution Thermospheric Imaging (MIGHTI) on the Ionospheric Connection Explorer (ICON) spacecraft, and four ground-based specular meteor radars (SMRs). Using the green-line MIGHTI channel, we analyze 1158 coincidences between Dec 2019 and May 2020 in the altitude range from 94 to 104 km where the observations overlap. We find that the two datasets are strongly correlated (r = 0.82) with a small mean difference (4.5 m/s). Although this agreement is good, an analysis of known error sources (e.g., shot noise, calibration errors, and analysis assumptions) can only account for about a quarter of the disagreement variance. The unexplained variance is 27.8% of the total signal variance and could be caused by unknown errors. However, based on an analysis of the spatial and caused by temporal variability of the wind on scales ≲70 min. The observed magnitudes agree well during temporal averaging of the two measurement modalities, we suggest that some of the disagreement is likely the night, but during the day, MIGHTI observes 16%-25% faster winds than the SMRs. This remains unresolved but is similar in certain ways to previous SMR-satellite comparisons.

Incorporation of cooked barley residue and spent grapes in the ration of pregnant Large White Yorkshire sows and their piglets.

In Kerala and other South Indian States, the ayurvedic by-products are widely available which can adequately be incorporated into pig diets. The objective of this study was to determine the effect of dietary incorporation of cooked barley residue and spent grapes on feed intake, growth performance, and nutrient utilization in sows and their piglets. Using completely randomized design (CRD), fifteen (15) Large White Yorkshire (LWY) pregnant sows with an average body weight of 180.53 kg, 3 weeks before the estimated date of farrowing, were randomly allotted to one of three treatment groups. The dietary treatment groups consisted of T1 (control), fed with a ration containing maize and soya bean meal of 18% CP and 3265 kcal/kg ME, T2 with a ration containing cooked barley residue replacing 25% maize in control, and T3 with a ration containing spent grapes replacing 25% maize in control ration. Data on average daily feed intake (ADFI), fortnightly bodyweight of sows and their piglets were recorded for 63 days. Also, the average daily gain (ADG), feed conversion ratio (FCR), and cost of feed per kg of weight gain of the piglets were calculated. The result showed no significant (P > 0.05) dietary treatment effects on average weekly dry matter intake and fortnightly average body weight gain among the sows in all the three groups. Piglets showed no significant difference (P > 0.05) in total dry matter intake (DMI), total body weight gain, ADG, and FCR among the three groups during the entire study period. No variations were observed for the apparent digestibility of nitrogen-free extract and the availability of magnesium in the three rations. The cost of feed per kg weight gain was lower in piglets belonging to T2 and T3 than the control (T1), though not significant statistically (P > 0.05). It is, therefore, concluded that cooked barley residue and spent grapes could be included up to 25% in the ration of sow and their piglets by partially replacing maize without affecting their overall performances.

Color and molecular structure alterations of brazilein extracted from Caesalpinia sappan L. under different pH and heating conditions.

Brazilein extract from sappan wood (Caesalpinia sappan L.) has potential for use as natural food colorant since it has no unique flavor and taste. Although brazilein has long been applied in several traditional foods and beverages, information on its stability, which is of importance for practical application, is still limited. In this work, brazilein was isolated from sappan wood; its purity was confirmed by nuclear magnetic resonance spectroscopy. Relations between molecular structures and color as well as thermal stabilities of brazilein in aqueous solutions at pH 3, 7 and 9 were for the first time investigated. At the lowest pH, zero net-charge structure of brazilein, which exhibited yellow color, was predominantly found. The deprotonated and fully deprotonated structures of brazilein, which exhibited orange and red colors, respectively, were found when pH of the aqueous solutions increased. The forms of brazilein existing at the higher pH suffered extensive degradation upon heating, while the form existing at the lowest pH possessed higher stability. Heat-induced deprotonation and degradation were confirmed by UV-visible and Fourier-transform infrared spectra as well as losses of brazilein content.

Nephroprotective effect of Combretum micranthum G. Don in nicotinamide-streptozotocin induced diabetic nephropathy in rats: In-vivo and in-silico experiments.

ETHNOPHARMACOLOGICAL RELEVANCE: Combretum micranthum G. Don (CM) is extensively used in traditional medicine throughout West Africa and commonly known as "long-life herbal tea" or "plant to heal". Further, traditional healers frequently use the title plant to mitigate of renal disorders. AIM OF THE STUDY: To explore the nephroprotective property of standardised hydroalcoholic extract of Combretum micranthum in nicotinamide-streptozotocin induced diabetic nephropathy in rats. In addition, in-silico computational experiments were performed with bioactive compounds of the title plant against PPARα and PPARγ. MATERIAL AND METHODS: Male rats were made diabetic by a single intraperitoneal (ip) injection of STZ (50 mg/kg), 15 min after ip administration of NA (100 mg/kg) dissolved in normal saline. The diabetic rats received CM extract (200 and 400 mg/kg p.o.) daily, for eight weeks. Body weights and blood glucose (non-fasting and fasting) of rats were measured weekly. Daily food and water consumption were also measured. After 8 weeks of treatment, urine biochemical parameters such as N-Acetyl-ß-D-Glucosaminidase (NAG), urea (UR), uric acid (UA), creatinine (CRE), and serum markers of diabetes, kidney damage and liver damage such as insulin, lipid parameters), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (γGT), albumin (Alb), magnesium (Mg2+), calcium (Ca2+), phosphorus (P), were estimated. Blood glycosylated hemoglobin (HbA1C) were also estimated. kidney and liver were used for biochemical estimation of oxidative stress markers such as lipid peroxidation, superoxide dismutase (SOD) activity and glutathione peroxidase (GPx) activity. The kidney and pancreas were used for histopathological study. Further, HPLC chemoprofiling of CM extract and in-silico molecular simulation experiments were performed. RESULTS: At the end of eight weeks, renal damage induced by the consequence of prolong diabetic condition was confirmed by altered levels of serum and urine kidney and liver function markers, oxidative stress markers and histopathological variations in kidney. Treatment with CM extract ameliorated the diabetes mellitus-induced renal biochemical parameters and histopathological changes. Further, HPLC-UV & MS experiments revealed that CM extract contains several bioactive compounds including hyperozide (62.35 µg/mg of extract) and quercitrin (19.07 µg/mg of extract). In-silico experiment exhibited cianidanol (-17.133), epicatechin (-15.107) exhibited higher docking score against PPARα and luteoforol (-11.038), epigallocatechin (-10.736) against PPARγ. Based on docking and drug likeness score, four bioactive compounds were selected for molecular dynamic experiments. Cianidanol and epigallocatechin out of the 30 compounds are concluded as a potential candidate for the treatment of DN through activating PPARα and PPARγ target protein. CONCLUSIONS: Taken together, the present study provided the scientific footage for the traditional use of Combretum micranthum.

Hydroxychavicol sensitizes imatinib-resistant chronic myelogenous leukemia cells to TRAIL-induced apoptosis by ROS-mediated IAP downregulation.

The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of cytokine superfamily, induces apoptosis in a number of tumor cells through the activation of extrinsic apoptotic pathway but shows little or no cytotoxicity toward normal cells. However some tumor cells are inherently resistant to TRAIL-mediated apoptosis, which needs to be addressed to establish TRAIL as a potential chemotherapeutic drug. In this study, our aim was to manipulate TRAIL-apoptosis pathway by hydroxychavicol (HCH), a polyphenol from Piper betel leaf, for the induction of apoptosis in TRAIL resistant chronic myeloid leukemia cell. When imatinib-resistant K562 cells were treated with HCH, it made these K562 cells sensitive to TRAIL. It was observed that HCH downregulated antiapoptotic proteins XIAP and FLIP, whereas the expression of TRAIL receptors, DR4 and DR5, remains unchanged. Moreover, we observed that reactive oxygen species or ROS played a crucial role in the downregulation of FLIP and XIAP because ROS scavenger significantly reversed the decrease of XIAP, and FLIP. Ubiquitin-proteasome pathway was observed to play a crucial role in the downregulation of XIAP and FLIP, as proteasomal inhibitor MG132 significantly reversed the downregulation of XIAP and FLIP. In conclusion, this study demonstrates the combinatorial treatment of TRAIL and HCH as promising alternative therapeutic approach to treat the imatinib-resistant leukemia, which are also resistant to TRAIL.

Primary Healthcare-based Diabetes Registry in Puducherry: Design and Methods.

BACKGROUND: Diabetes registries monitor the population prevalence and incidence of diabetes, monitor diabetes control program, provide information of quality of care to health service providers, and provide a sampling frame for interventional studies. This study documents the process of establishing a prospective diabetes registry in a primary health-care setting in Puducherry. METHODS: This is a facility-based prospective registry conducted in six randomly selected urban health centers in Puducherry, with enrollment of all known patients with diabetes attending chronic disease clinics. Administrative approvals were obtained from Government Health Services. Manuals for training of medical officers, health-care workers, and case report forms were developed. Diabetes registry was prepared using Epi Info software. RESULTS: In the first phase, demographic characteristics, risk factors, complications, coexisting chronic conditions, lifestyle and medical management, and clinical outcomes were recorded. Around 2177 patients with diabetes have been registered in six Primary Health Centres out of a total of 2948 participants seeking care from chronic disease clinic. Registration coverage ranges from 61% to 105% in these centers. CONCLUSION: This study has documented methodological details, and learning experiences gained while developing a diabetes registry at the primary health care level and the scope for upscaling to a Management Information System for Diabetes and a State-wide Registry. Improvement in patient care through needs assessment and quality assurance in service delivery is an important theme envisioned by this registry.

Optimization and characterization of hydrochar produced from microwave hydrothermal carbonization of fish waste.

Fish processing results in large amounts of solid and liquid wastes that are unsustainably dumped into oceans and landfills. Alternative sustainable technologies that completely utilize seafood wastes are needed. Hydrothermal carbonization (HTC) that converts moisture-rich biomass into hydrochar is mostly employed for pure lignocellulosic biowaste. However, the suitability of HTC for pure non-lignocellulosic waste is unknown. Here, for the first time, a response surface design guided optimization of microwave hydrothermal carbonization (MHTC) process parameters, holding temperature (150-210°C) and time (90-120min), showed that a temperature of approximately 200°C and a time of approximately 119min yielded maximal hydrochar (∼34%). The atomic carbon and ash content, and calorific value of hydrochar were approximately 25-57%, 20-28%, and 19-24.5MJ/kg respectively, depending on the MHTC operating conditions. Taken together, these results confirm that MHTC produces hydrochar from fish waste of quality comparable to one produced from certain lignocellulosic, sewage and municipal wastes. Therefore, this strategy presents an exciting alternative technology that can be used either independently or in combination with other valorization techniques to completely utilize fish wastes irrespective of their quality.

Synthesis of thiazolidine-2,4-dione derivatives: anticancer, antimicrobial and DNA cleavage studies.

In the search of efficient anticancer agents, here, new 5-(4-alkylbenzyledene)thiazolidine-2,4-dione derivatives (5a-g) have been successfully synthesized and characterized and are evaluated for anticancer and antimicrobial activities using DNA cleavage studies. In vitro studies on anticancer activity of compound 5d (NSC: 768619/1) was done against the full panel of 60 human tumor cell lines. The five-level dose activity results revealed that, the compound 5d was active against all the cell lines, it has shown potential activity against leukemia SR (GI50: 2.04 µM), non-small cell lung cancer NCI-H522 (GI50: 1.36 µM), colon cancer COLO 205 (GI50: 1.64 µM), CNS cancer SF-539 (GI50: 1.87 µM), melanoma SK-MEL-2 (GI50: 1.64 µM), ovarian cancer OVCAR-3 (GI50: 1.87 µM), renal cancer RXF 393 (GI50: 1.15 µM), prostate cancer PC-3 (GI50: 1.90 µM), and breast cancer MDA-MB-468(GI50: 1.11 µM). DNA cleavage studies revealed that at 50 µg/mL concentration, partial DNA digestion was observed and when the concentration is increasing to threefold (150 µg/mL), complete linear DNA digestion and partial supercoiled DNA digestion was observed. Further antimicrobial studies indicate that all the synthesized compounds except compound 5a possess prominent activity against all the screened microbial species. This study throws a ray of light in the field of anticancer drugs.

Photochemical synthesis and anticancer activity of barbituric acid, thiobarbituric acid, thiosemicarbazide, and isoniazid linked to 2-phenyl indole derivatives.

2-Phenyl-1H-indole-3-carbaldehyde-based barbituric acid, thiobarbituric acid, thiosemicarbazide, isoniazid, and malononitrile derivatives were synthesized under photochemical conditions. The antitumor activities of the synthesized compounds were evaluated on three different human cancer cell lines representing prostate cancer cell line DU145, Dwivedi (DWD) cancer cell lines, and breast cancer cell line MCF7. All the screened compounds possessed moderate anticancer activity, and out of all the screened compounds, 5-{1[2-(4-chloro-phenyl)2-oxo-ethyl]-2-phenyl-1H-indole-3-ylmethylene}-2-thioxo-dihydro-pyrimidine-4,6-dione (2b) and 5-{1[2-(4-methoxy-phenyl)2-oxo-ethyl]-2-phenyl-1H-indole-3-ylmethylene}-2-thioxo-dihydro-pyrimidine-4,6-dione (2d) exhibited marked antitumor activity against used cell lines. Additionally, barbituric acid derivatives were selective to inhibit cell line DWD and breast cancer cell lines.

Effect of C/N ratio and salinity on power generation in compost microbial fuel cells.

In this work, compost Microbial Fuel Cells (cMFCs) were used to generate electricity from a mix of fruit and vegetable wastes, and soil with different C/N ratios and salinities. Experiments were carried out in 500mL cMFCs equipped with carbon felt anodes and manganese dioxide cathodes. The cMFCs were loaded with fresh compost and operated at 20-23°C for up to 97days. The low C/N ratio (C/N 24) had a greater power production with a maximum power density of 5.29mW/m(2) (71.43mW/m(3)), indicating a more favorable condition for microbial growth. High-saline cMFCs produced lower power, suggesting that their level of salinity (10g/L of NaCl) inhibited electricigenic microorganisms. The closed-circuit cMFC showed an improved degradation of organic matter by 6% to 8% compared to the control MFC operated in an open circuit mode (no external resistor attached).

Enhancing shelf life of minimally processed multiplier onion using silicone membrane.

The aim of storage of minimal processed product is to increase the shelf life and thereby extend the period of availability of minimally processed produce. The silicone membrane makes use of the ability of polymer to permit selective passage of gases at different rates according to their physical and chemical properties. Here, the product stored maintains its own atmosphere by the combined effects of respiration process of the commodity and the diffusion rate through the membrane. A study was undertaken to enhance the shelf life of minimally processed multiplier onion with silicone membrane. The respiration activity was recorded at a temperature of 30 ± 2 °C (RH = 60 %) and 5 ± 1 °C (RH = 90 %). The respiration was found to be 23.4, 15.6, 10 mg CO2kg(-1)h(-1) at 5 ± 1 °C and 140, 110, 60 mg CO2kg(-1) h(-1) at 30 ± 2° for the peeled, sliced and diced multiplier onion, respectively. The respiration rate for the fresh multiplier onion was recorded to be 5, 10 mg CO2kg(-1) h(-1) at 5 ± 1 °C and 30 ± 1 ° C, respectively. Based on the shelf life studies and on the sensory evaluation, it was found that only the peeled multiplier onion could be stored. The sliced and diced multiplier onion did not have the required shelf life. The shelf life of the multiplier onion in the peel form could be increased from 4-5 days to 14 days by using the combined effect of silicone membrane (6 cm(2)/kg) and low temperature (5 ± 1 °C).

Expression of podoplanin in oral premalignant and malignant lesions and its potential as a biomarker.

AIM: To analyze and compare the expression of podoplanin in normal oral tissues, leukoplakia and oral squamous cell carcinoma (OSCC) and to predict its use as a biomarker. MATERIALS AND METHODS: Ninety-two formalin fixed paraffin embedded tissue samples comprising of 32 cases of leukoplakia, 50 cases of OSCCs and ten normal gingival samples. The samples were retrieved from archives and immunohistochemically analyzed using podoplanin. Appendix tissue samples were used for control purposes. The results were tabulated and statistically analyzed using ANOVA/Kruskal-Wallis test with post-hoc tests, where demographic details are compared and analyzed using Pearson Chi-square test. RESULTS: The study results showed, absence of podoplanin expression in the epithelium of all the gingival samples (Group I). Positive podoplanin expression noticed in 19 out of 32 (59.4%) cases of leukoplakia (Group II) and 41 out of 50 (82%) cases of OSCCs (Group III). The expression of podoplanin among different groups was highly significant (P = 0.000). CONCLUSION: The podoplanin may be considered as a predictor marker in assessing malignant transformation of premalignancies and prognosis of oral malignancy. Indeed it is believed that podoplanin might play a role in tumor progression though exact mechanism is not fully elucidated. Further research is required to understand its exact pathophysiology.

Evaluation of chromogenic media in detection of vancomycin resistant enterococci.

INTRODUCTION: Vancomycin resistant Enterococci have become important nosocomial pathogens. So it is necessary to monitor continuously such infections in the hospitals. MATERIALS AND METHODS: A total of 100 Enterococci isolated from 4489 various clinical samples were speciated and antibiogram was done according to standard laboratory methods. The efficacy of CHROMagar(TM) VRE (France) and Hicrome VRE (Himedia) in detecting VRE was evaluated using E- test (Himedia). RESULTS: Hicrome VRE and CHROMagar(TM) VRE showed sensitivity of 100% and specificity of 99% as compared to E-test. CONCLUSION: In the present study VRE was not isolated. Prudent use of vancomycin and continuous surveillance for VRE will prevent the emergence of vancomycin resistant Enterococci in the locality in future. Identification of VRE by chromogenic media is rapid, easy to perform, cost effective compared to technically demanding, time consuming and costly conventional method.

Asymmetric synthesis of drug-like spiro[chroman-3,3'-indolin]-2'-ones through aminal-catalysis.

Asymmetric synthesis of drug-like functionalized spiro[chroman-3,3'-indolin]-2'-ones 5 containing three contiguous stereocenters with high diastereo- and enantioselectivities was achieved using the reflexive-Michael (r-M) reaction of unmodified hydroxyenals 1 with various (E)-3-alkylideneindolin-2-ones 2 in the presence of (R)-DPPOTMS/AcOH (R)-3/4b as a catalyst at room temperature. Chiral spiro[chroman-3,3'-indolin]-2'-ones 5 were transformed into the functionalized spiranes 7, 9, and 10 in good yields with high selectivity through Wittig, TCRA, acetal protection and reduction reactions, respectively. Supporting evidence for the reaction pathway through the formation of the important catalytic species of "aminals" was observed through NMR and ESI-HRMS analysis of an ongoing reaction between 1 and (R)-3 in CDCl3 and also shown by the structural requirement in hydroxyenals 1 to generate the "aminals" with (R)-3 through controlled experiments.

Pharmacognostic and phytochemical investigation of the leaves of Malvastrum coromandelianum (L.) Garcke.

BACKGROUND: Malvastrum coromandelianum belongs to the family Malvaceae, commonly known as false mallow. Ethnobotanical survey revealed that it is used to treat various disorders. Pharmacological screening revealed that the plant possess antinoceceptive, anti-inflammatory, analgesic, and antibacterial activities. Lack of standardization parameters for herbal raw material is a great hindrance in ensuring the purity of M. coromandelianum. The present work was taken up to with a focus to set standardization parameters for M. coromandelianum. MATERIALS AND METHODS: The plant was subjected to macroscopic and microscopic studies. Physicochemical parameters such as ash value and extractive value were determined by standard procedures. Different extracts were screened for the presence of secondary metabolites. Phenolic and flavonoid contents were estimated. Plant was subjected for high performance thin layer chromatography (HPTLC) analysis using standard chromatographic procedure. RESULT: The microscopic characteristics showed the dorsiventral nature of leaf. Two types of trichomes were observed: Covering, unicellular, uniseriate, and bi-cellular head sessile glandular. Vascular bundle was surrounded by spongy parenchyma. Phytochemical screening revealed the presence alkaloids, tannins, amino acid proteins, and carbohydrates. The phenolic and flavonoid content estimation revealed the presence of appreciable amount of these constituents, while HPTLC analysis showed the presence of ß-sitosterol in petroleum ether extract. CONCLUSION: These findings will be useful for the establishment of standardization parameters for M. coromandelianum.