ABSTRACT
Humanized mouse models are increasingly studied to recapitulate human-like bone physiology. While human and mouse bone architectures differ in multiple scales, the extent to which chimeric human-mouse bone physiologically interacts and structurally integrates remains unknown. Here, we identify that humanized bone is formed by a mosaic of human and mouse collagen, structurally integrated within the same bone organ, as shown by immunohistochemistry. Combining this with materials science techniques, we investigate the extracellular matrix of specific human and mouse collagen regions. We show that human-like osteocyte lacunar-canalicular network is retained within human collagen regions and is distinct to that of mouse tissue. This multiscale analysis shows that human and mouse tissues physiologically integrate into a single, functional bone tissue while maintaining their species-specific ultrastructural differences. These results offer an original method to validate and advance tissue-engineered human-like bone in chimeric animal models, which grow to be eloquent tools in biomedical research.
ABSTRACT
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Subject(s)
Humans , Female , Middle Aged , Aged , Dermoscopy , Skin Neoplasms/pathology , Skin Neoplasms/secondary , Carcinoma, Basal Cell/diagnosis , Neoplasm Metastasis/pathology , Breast Neoplasms/pathology , Carcinoma, Basal Cell/pathology , Scalp/pathology , Scapula/pathologyABSTRACT
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Subject(s)
Humans , Male , Female , Adult , Middle Aged , Herpes Zoster/complications , Herpes Zoster/epidemiology , Acyclovir/administration & dosage , Skin Diseases, Viral/pathology , Emergency Medical Services , Zinc Sulfate/administration & dosage , Fusidic Acid/administration & dosage , Antiviral Agents/administration & dosage , Administration, Topical , Herpes Zoster/pathology , Skin Diseases, Viral/drug therapySubject(s)
Antineoplastic Agents/adverse effects , Drug Eruptions/diagnosis , Drug Eruptions/etiology , Exanthema/diagnosis , Exanthema/etiology , Phenylurea Compounds/adverse effects , Protein Kinase Inhibitors/adverse effects , Pyridines/adverse effects , Antineoplastic Agents/administration & dosage , Antineoplastic Combined Chemotherapy Protocols/adverse effects , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Desensitization, Immunologic , Drug Eruptions/therapy , Exanthema/therapy , Female , Humans , Liver Neoplasms/complications , Liver Neoplasms/drug therapy , Middle Aged , Phenylurea Compounds/administration & dosage , Protein Kinase Inhibitors/administration & dosage , Pyridines/administration & dosage , Severity of Illness Index , Treatment OutcomeSubject(s)
Herpes Zoster/pathology , Adult , Aged , Herpes Zoster/diagnosis , Herpes Zoster/drug therapy , HumansABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Antineoplastic Agents/adverse effects , Drug Eruptions/diagnosis , Drug Eruptions/etiology , Exanthema/diagnosis , Exanthema/etiology , Phenylurea Compounds/adverse effects , Protein Kinase Inhibitors/adverse effects , Pyridines/adverse effects , Antineoplastic Agents/administration & dosage , Antineoplastic Combined Chemotherapy Protocols , Desensitization, Immunologic , Drug Eruptions/therapy , Exanthema/therapy , Liver Neoplasms/drug therapy , Pyridines/administration & dosage , Severity of Illness Index , Treatment OutcomeABSTRACT
No disponible
Subject(s)
Image Processing, Computer-Assisted/methods , Photograph/instrumentation , Dermatology/trends , Dermoscopy , Lenses , Lighting/instrumentation , Diagnostic Imaging/instrumentationABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Skin Diseases, Vesiculobullous/diagnosis , Tongue Diseases/diagnosis , Mouth Mucosa/injuries , Oral Hemorrhage/etiologySubject(s)
Anesthesia, Epidural/instrumentation , Catheterization , Adult , Equipment Failure , Female , HumansABSTRACT
No disponible