An Evaluation of Thiol/Disulphide Homeostasis and HDL Values in Patients with Cholelithiasis: A Prospective Case-control Study.

OBJECTIVE: To determine the changes in thiol/disulphide homeostasis in patients determined with asymptomatic cholelithiasis and to investigate any potential correlation between these thiol-disulphide parameters and HDL cholesterol. STUDY DESIGN: A descriptive, comparative study. PLACE AND DURATION OF STUDY: Ankara City Hospital between 15 September and 31 December 2019. METHODOLOGY: This study included 42 patients aged 18-70 years, who presented at the Gastroenterology Clinic and were diagnosed with cholelithiasis on ultrasound examination. A control group was formed of 51 healthy volunteers aged 18-70 years. Thiol/disulphide homeostasis and HDL cholesterol was noted and compared between the groups. RESULTS: The mean age was 44.16 ± 13.35 years in cholelithiasis patient group and 31.88±13.27 years in the control group. The triglyceride, VLDL, total cholesterol/HDL, and non-HDL levels were significantly higher and HDL level was significantly low in the patient group (both p <0.05). Regarding thiol-disulphide balance, native thiol, total thiol, and albumin values were found to be statistically significantly low in the patient group (p <0.05), and the IMA, index-1, index-2, and index-3 values were significantly high (p <0.05). CONCLUSION: The oxidant/antioxidant balance shifted towards oxidation in patients with asymptomatic gallstones. The non-HDL value was increased. There was a positive correlation between the thiol-disulphide parameters and the non-HDL value. The increasing non-HDL amount could be effective in the pathogenesis of gallstone disease by disrupting the oxidative balance. KEY WORDS: Cholelithiasis, Thiol-disulphide homeostasis, Lipid profile, Non-HDL, HDL, Oxidative stress.

Effects of Oxidant-Antioxidant and Vitamin D Levels on Clinical and Laboratory Data in Children With Fatty Liver Disease.

Background and Aims Fatty liver increases oxidative stress and may trigger antioxidant mechanisms. We aimed to compare the levels of vitamin D, which has antioxidant properties, as well as total oxidant status (TOS), total antioxidant status (TAS), and catalase between patients with nonalcoholic fatty liver (NAFL) and the control group. Methods We compared vitamin D, TOS, TAS, catalase levels, and other biochemical parameters between pediatric patients with ultrasonographically detected NAFL and an age-matched healthy control group. Results NAFL patients had a significantly lower vitamin D level (p < 0.001). The patient group also had significantly greater height, weight, body mass index (BMI) Z score, parathyroid hormone, triglyceride, glucose, antioxidant (TAS and catalase), and TOS levels compared to the controls (p ≥ 0.001). There was no significant difference between the obese and non-obese NAFL patients with respect to TAS, TOS, catalase levels, and other biochemical parameters (p < 0.05). There was a positive correlation between height, weight, BMI Z score, and hepatosteatosis grade, and TAS, TOS, and catalase levels, and a negative correlation with vitamin D level. We also found a negative correlation between vitamin D level and TOS and catalase level. Conclusions Our study revealed lower levels of vitamin D and higher levels of oxidant-antioxidants including TOS, TAS, and catalase in patients with NAFL.