The concentration of LEAP2 in human milk and infant plasma is positively associated with adiposity and body weight in the first year of life.

BACKGROUND: The liver-expressed antimicrobial peptide 2 (LEAP2) is a recently recognized anorectic and glucose-regulating hormone, with unknown role in lactation. OBJECTIVES: 1) Assess LEAP2 presence in human milk and putative associations with infant body weight and adiposity in the first year of life, 2) Evaluate the impact of maternal weight status on LEAP2 concentration and 3) Explore the relationship between infant plasma LEAP2 concentration and body weight and adiposity. METHODS: This prospective cohort observational study assessed LEAP2 concentration in plasma and milk from lactating women with normal weight (n=26) or overweight/obesity (OW/OB, n=26) at six months postpartum and in 6-month-old infant plasma, examining associations with metabolic and anthropometric variables at 6 months and 1 year. Maternal plasma and milk leptin and insulin concentrations were also measured. LEAP2 expression in milk fat globules and single-cell-RNA-sequencing datasets was evaluated. RESULTS: LEAP2 was detected in all milk samples assessed (2.08±0.65 ng/ml) and was positively associated with infant triceps (p=0.022, Cohen f2=1.25) and subscapular (p=0.008, f2=0.68) skinfolds at 1 year old. Maternal LEAP2 was positively associated with insulin (p=0.005, f2=0.30) and pre-pregnancy body mass index (BMI) (p=0.040, f2=0.17) and negatively associated with gestational weight gain (p=0.008, f2=0.25) and postpartum weight retention (p=0.036, f2=0.15). Maternal LEAP2 was higher in plasma (p=0.039), but not milk of lactating women with OW/OB. Infant plasma LEAP2 (1.98±0.28 ng/ml) was positively associated with weight (p=0.004, f2=0.63), BMI (p=0.049, f2=0.37), and weight-for-length (p=0.024, f2=0.35) z-scores at 1 year old, predominantly in males. No evidence of LEAP2 mRNA expression was found in mammary cells. CONCLUSIONS: Milk LEAP2 is a bioactive component that plays a role in infant fat accretion in the first year of life. While maternal LEAP2 responds to weight change in pregnancy and lactation, infant plasma LEAP2 might be involved in body weight regulation in early life. CLINICAL TRIAL REGISTRY: This study was registered at clinicaltrials.gov as NCT05798676. https://clinicaltrials.gov/study/NCT05798676.

Blood Lead Levels and Their Association with Iron Deficiency and Anemia in Children.

Lead is a ubiquitous and neurotoxic heavy metal particularly harmful to children, who are more susceptible than adults to its toxic effects. The prevalence of lead poisoning and iron deficiency (ID) is high in socioeconomically vulnerable child populations, negatively affecting neurocognitive development in children. Although numerous studies have shown the association between blood lead levels (BLL) and ID, the issue remains controversial. Here, we aimed to identify the association between BLL and iron nutritional status in children. We conducted an analytical cross-sectional study of healthy children aged 1-6 y attending periodic health checks in primary healthcare units from La Plata and its surroundings, Buenos Aires, Argentina, between 2012 and 2017. We performed anthropometric evaluations and determined BLL, hemoglobin (Hb) and serum ferritin levels. Blood lead levels ≥ 5 µg/dL were defined as elevated BLL; ferritin levels < 12 ng/ml were considered ID; Hb levels < 11 g/dL (< 11.5 g/dL for children ≥ 5 y) were defined as anemia. Data were analysed using Mann Whitney test, Student´s t-test, chi-square test and logistic regression. The R package (v. 4.2.2.) was used for the statistical analysis of data. The sample included 392 children (mean age, 2.4 ± 1.4 y; 44.6% females). The prevalence of elevated BLL, ID and anemia was 8.7%, 26.3% and 31.8%, respectively. We found a significant association between elevated BLL and ID (odds ratio [OR], 95% confidence interval [CI]: 3.16 (1.50, 6.63)). The prevalence of elevated BLL was 16.2% and 5.8% in children with and without ID, respectively (p = 0.003). We also found association between elevated BLL and anemia (OR 95% CI: 3.03 (1.49, 6.29)). In conclusion, blood lead levels ≥ 5 µg/dL were significantly associated with ID and anemia in children aged 1-6 years.

Leptin receptor expression in blood mononuclear cells of lactating women is associated with infant body weight: Potential role as a molecular biomarker.

BACKGROUND: Molecular biomarkers of maternal leptin resistance associated with infant weight are needed. OBJECTIVES: To evaluate gene expression of leptin receptor (LEPR), suppressor of cytokine signalling 3 (SOCS3) and insulin receptor in peripheral blood mononuclear cells (PBMCs) of lactating women and their relationship with infant body weight and adiposity. METHODS: At day 10 postpartum, maternal gene expression in PBMCs as well as leptin and insulin concentrations in plasma and milk were assessed (n = 68). Infant weight and BMI z-scores, skinfolds and arm circumference were obtained at 10 days and/or at 3 months old. RESULTS: In mothers with pre-pregnancy overweight or obesity (OW/OB), LEPR expression was reduced (p = 0.013) whereas plasma and milk leptin and milk insulin concentrations were elevated. LEPR expression was positively related with infant weight z-score (Beta (95% CI): 0.40 (0.17, 0.63), p = 0.001) but not with leptin concentrations. SOCS3 expression was positively related with infant weight z-score (Beta (95% CI): 0.28 (0.04, 0.51), p = 0.024) and arm circumference (Beta (95% CI): 0.57 (0.32, 0.82), p < 0.001). Relationships remained significant after adjusting for maternal and infant confounders. CONCLUSIONS: LEPR and SOCS3 gene expression in PBMCs are novel maternal molecular biomarkers that reflect leptin resistance and are associated with infant body weight and adiposity.

Efectividad de la administración de hierro en forma semanal y diaria para la prevención de anemia por deficiencia de hierro en lactantes / Effectiveness of weekly and daily iron administration for the prevention of iron deficiency anemia in infants

Introducción. La deficiencia de hierro (DH) es la carencia nutricional más prevalente y la principal causa de anemia en lactantes. Existe consenso en la suplementación diaria con hierro como estrategia de prevención; también se demostró que la suplementación semanal es eficaz, pero la evidencia en lactantes es escasa. El objetivo fue comparar la efectividad de la administración diaria de hierro frente a la semanal para la prevención de la anemia por DH del lactante. Población y métodos. Ensayo clínico controlado y aleatorizado. Lactantes atendidos en un centro de salud público, sin anemia a los 3 meses de edad, aleatorizados en tres grupos: suplementación diaria (1 mg/kg/día), semanal (4 mg/kg/semana) o sin suplementación (grupo control con lactancia materna exclusiva [LME]). Se evaluó anemia y DH a los 3 y 6 meses. Se registró grado de adherencia y efectos adversos. Los datos se analizaron con el software R versión 4.0.3. Resultados. Participaron 227 lactantes. A los 6 meses el grupo de lactantes con LME sin suplementación (control) presentó prevalencias de DH y anemia por DH (ADH) mayores que los grupos intervenidos (diario y semanal). DH: 40,5 % vs. 13,5 % y 16,7 % (p = 0,002); ADH: 33,3 % vs. 7,8 % y 10 % (p < 0,001). No hubo diferencias entre los grupos diario y semanal. Tampoco hubo diferencias en el porcentaje de alta adherencia a la suplementación (50,6 % diaria vs. 57,1 % semanal), ni en los efectos adversos. Conclusiones. No se hallaron diferencias significativas en la efectividad entre la administración diaria y semanal para la prevención de ADH del lactante.

Introduction. Iron deficiency (ID) is the most prevalent nutritional deficiency and the main cause of anemia in infants. There is consensus on daily iron supplementation as a preventive strategy; and weekly iron supplementation has also been shown to be effective, but evidence in infants is scarce. The objective of this study was to compare the effectiveness of daily versus weekly iron administration for the prevention of ID anemia (IDA) in infants. Population and methods. Randomized, controlled clinical trial. Infants seen at a public health center, without anemia at 3 months of age, were randomized into 3 groups: daily supplementation (1 mg/kg/ day), weekly supplementation (4 mg/kg/week), or no supplementation (control group with exclusive breastfeeding [EB]). Anemia and ID were assessed at 3 and 6 months old. Adherence and adverse events were recorded. Data were analyzed using the R software, version 4.0.3. Results. A total of 227 infants participated. At 6 months, the group of infants with EB without supplementation(control) had a higher prevalence of ID and IDA than the intervention groups (daily and weekly). ID: 40.5% versus 13.5% and 16.7% (p = 0.002); IDA: 33.3% versus 7.8% and 10% (p < 0.001). There were no differences between the daily and weekly supplementation groups. There were also no differences in the percentage of high adherence to supplementation (50.6% daily versus 57.1% weekly) or adverse events. Conclusions. No significant differences in effectiveness were observed between daily and weekly administration for the prevention of infant IDA.

Effectiveness of weekly and daily iron administration for the prevention of iron deficiency anemia in infants. / Efectividad de la administración de hierro en forma semanal y diaria para la prevención de anemia por deficiencia de hierro en lactantes.

Introduction. Iron deficiency (ID) is the most prevalent nutritional deficiency and the main cause of anemia in infants. There is consensus on daily iron supplementation as a preventive strategy; and weekly iron supplementation has also been shown to be effective, but evidence in infants is scarce. The objective of this study was to compare the effectiveness of daily versus weekly iron administration for the prevention of ID anemia (IDA) in infants. Population and methods. Randomized, controlled clinical trial. Infants seen at a public health center, without anemia at 3 months of age, were randomized into 3 groups: daily supplementation (1 mg/kg/day), weekly supplementation (4 mg/kg/week), or no supplementation (control group with exclusive breastfeeding [EB]). Anemia and ID were assessed at 3 and 6 months old. Adherence and adverse events were recorded. Data were analyzed using the R software, version 4.0.3. Results. A total of 227 infants participated. At 6 months, the group of infants with EB without supplementation (control) had a higher prevalence of ID and IDA than the intervention groups (daily and weekly). ID: 40.5% versus 13.5% and 16.7% (p = 0.002); IDA: 33.3% versus 7.8% and 10% (p < 0.001). There were no differences between the daily and weekly supplementation groups. There were also no differences in the percentage of high adherence to supplementation (50.6% daily versus 57.1% weekly) or adverse events. Conclusions. No significant differences in effectiveness were observed between daily and weekly administration for the prevention of infant IDA.

Introducción. La deficiencia de hierro (DH) es la carencia nutricional más prevalente y la principal causa de anemia en lactantes. Existe consenso en la suplementación diaria con hierro como estrategia de prevención; también se demostró que la suplementación semanal es eficaz, pero la evidencia en lactantes es escasa. El objetivo fue comparar la efectividad de la administración diaria de hierro frente a la semanal para la prevención de la anemia por DH del lactante. Población y métodos. Ensayo clínico controlado y aleatorizado. Lactantes atendidos en un centro de salud público, sin anemia a los 3 meses de edad, aleatorizados en tres grupos: suplementación diaria (1 mg/kg/día), semanal (4 mg/kg/semana) o sin suplementación (grupo control con lactancia materna exclusiva [LME]). Se evaluó anemia y DH a los 3 y 6 meses. Se registró grado de adherencia y efectos adversos. Los datos se analizaron con el software R versión 4.0.3. Resultados. Participaron 227 lactantes. A los 6 meses el grupo de lactantes con LME sin suplementación (control) presentó prevalencias de DH y anemia por DH (ADH) mayores que los grupos intervenidos (diario y semanal). DH: 40,5 % vs. 13,5 % y 16,7 % (p = 0,002); ADH: 33,3 % vs. 7,8 % y 10 % (p < 0,001). No hubo diferencias entre los grupos diario y semanal. Tampoco hubo diferencias en el porcentaje de alta adherencia a la suplementación (50,6 % diaria vs. 57,1 % semanal), ni en los efectos adversos. Conclusiones. No se hallaron diferencias significativas en la efectividad entre la administración diaria y semanal para la prevención de ADH del lactante.

Prolonged body weight gain, lifestyle changes and health-related quality of life in children during the COVID-19 pandemic lockdown: A follow-up study.

BACKGROUND: Further investigation is needed to define the impact of long-term pandemic lockdown in children. OBJECTIVES: To examine changes in body mass index z-score (zBMI), lifestyle, Health-Related Quality of Life and proportion of overweight or obesity (OW/OB) in 6- to 9-year-old children in Argentina. METHODS: Observational study with baseline measurements prior to lockdown and follow-up after eight months of strict restrictive measures (November 2020, first visit, n = 144) and after ten months of partial reopening (September 2021, second visit, n = 108). Anthropometric changes from baseline to first visit in lockdown group (LG) were compared with a historical control group (HCG, n = 134). Follow-up visits included anthropometric measures, lifestyle questionnaire and Pediatric Quality of Life Inventory. RESULTS: Change in zBMI was higher in LG [median, IQR: 0.46 (-0.00; 0.83)] vs HCG [median, IQR: 0.02 (-0.31; 0.27)]; p < 0.001, particularly in children with pre-existing OW/OB. In LG, zBMI was higher at first and second visit vs baseline (p < 0.001) and in second visit vs first visit for boys (p = 0.037) but not for girls. The proportion of children with OW/OB increased from baseline (43.5%) to first (56.5%) and second visit (58.3%) (p = 0.029). Unlike girls, the proportion of boys with OW/OB increased from baseline to first and second visit (p = 0.045). Change in zBMI was higher in children with less healthy habits (p < 0.001). CONCLUSIONS: Weight gain continued to increase in boys when lockdown measurements were eased, although sedentary behaviors decreased and quality of life improved, indicating that the effects of pandemic lockdown could be difficult to reverse.

Neurodevelopmental assessment of infants born to mothers with hypertensive disorder of pregnancy at six months of age.

Infant neurodevelopment is a complex process which may be affected by different events during pregnancy, such as hypertensive disorders of pregnancy (HDP). We conducted a prospective cohort study to compare the prevalence of neurodevelopmental disorders in infants born to mothers with and without HDP at six months of age. Participants attended the Health Observatory of Instituto de Desarrollo e Investigaciones Pediátricas "Prof. Dr. Fernando E. Viteri" during 2018 and 2019. Infant neurodevelopment was assessed with the Bayley Scales of Infant and Toddler Development-Third Edition (Bayley-III). Data were analyzed using Chi-square, Student's t-test and Mann-Whitney test. Of the 132 participating infants, 68 and 64 were born to mothers with and without HDP, respectively. At six months, the prevalence of risk of neurodevelopmental delay was significantly higher in infants born to mothers with than without HDP (27.9% vs. 9.4%; p = 0.008) (odds ratio, 3.71; 95% confidence interval, 1.30; 12.28). In conclusion, infants born to mothers with HDP had three times increased risk of neurodevelopmental delay at six months of age.

Consequences of Lockdown During COVID-19 Pandemic in Lifestyle and Emotional State of Children in Argentina.

The implications of the coronavirus disease (COVID-19) lockdown measurements and social isolation in children and their parents are still unknown. The aims of this study were to examine the impact of COVID-19 lockdown on emotional state, feelings and lifestyle of children and their parents, to explore the association between parental characteristics and child well-being and to examine whether the impact of lockdown depends on socio-economic status. Parents completed an online survey including data about socio-demographic information, parent and child feelings and lifestyle during lockdown. Logistic regression and correlation analysis were used to establish associations between variables. In total, 814 parents with children between 4 and 11 were included in the study. According to parents, 69.5% of the children showed changes in their emotional state, 55.3% altered their routine and 62.6% showed sleep disorders. Families with lower socio-economic status were more worried about health, shortage of food and household income (p < 0.01). Parent and children concern about food/essential items were highly associated [OR (CI 95%) 13.0 (6.81, 26.5), p < 0.01]. Adverse children's emotional state was associated with parental feeling of loneliness (r = 0.35) and inversely associated with keeping a routine (r = -0.11). Sleep changes were inversely associated with keeping a routine and having a balcony/garden (r = -0.53 and -0.16). We conclude that lockdown affected emotional state and lifestyle of children and parents, which were strongly related. Routine and positive parental attitude supported children's well-being. Economic issues were an important concern in families with lower socio-economic status. Our findings can help to promote child health during lockdown.

Evaluación de la inteligencia sensoriomotriz y del desarrollo psicomotor en lactantes clínicamente sanos asistidos en el sector público de salud / Assessment of sensorimotor intelligence and psychomotor development in clinically healthy infants assisted in the public health sector

Introducción: La primera infancia es el período de mayor neuroplasticidad. La detección temprana de alteraciones del desarrollo permite la intervención oportuna para reducir secuelas y/o complicaciones. Objetivo: Evaluar el desarrollo psicomotor y cognitivo en lactantes asistidos en el sector público de salud, entre los 6 y 9 meses de edad. Población y métodos: Estudio descriptivo de seguimiento, de niños nacidos a término, clínicamente sanos en la evaluación, con peso, talla y perímetro cefálico adecuados para la edad. Se utilizó la Escala de Evaluación del Desarrollo Psicomotor y la Escala Argentina de Inteligencia Sensorio-motriz entre los 6 y 9 meses. En los casos en que se detectaron riesgo o retraso, se intervino con estimulación temprana. Se determinaron las prevalencias de riesgo/retraso del desarrollo psicomotor e inteligencia sensorio-motriz. Se utilizó el paquete estadístico R, versión 3.3.1. Resultados: Fueron evaluados 102 niños. A los 6 meses, el 22,5 % presentó riesgo/retraso en el desarrollo. Las prevalencias de resultados no esperados para la edad en desarrollo psicomotor y en inteligencia sensorio motriz fueron del 13,7 % y del 16,7 %, respectivamente. Estos niños recibieron estimulación temprana y todos mejoraron sus resultados. A los 9 meses, la prevalencia de riesgo/retraso fue del 20,6 %: el 14,7 % en desarrollo psicomotor y el 9,8 % en inteligencia sensorio motriz. Conclusión: El 22,5 % de los lactantes presentó riesgo/retraso del desarrollo a los 6 meses; la prevalencia a los 9 meses fue del 20,6 %. La evaluación temprana del desarrollo permitió detectar alteraciones e intervenir tempranamente.

Introduction: Infancy is the period of greatest neuroplasticity. The early detection of developmental disorders helps to provide a timely intervention aimed at reducing sequelae and/or complications. Objective: To assess psychomotor and cognitive development in infants between 6 and 9 months old assisted in the public health sector. Population and methods: Descriptive study with follow-up of full-term infants who were clinically healthy at the time of assessment and had an adequate weight, height, and head circumference for their age. The Assessment Scale of Psychomotor Development and the Argentine Scale of Sensorimotor Intelligence were used in infants aged 6-9 months. Early stimulation was the intervention provided to those who showed a risk or delay. The prevalence of risk/delay of psychomotor development and sensorimotor intelligence was determined. The statistical software package R, version 3.3.1 was used. Results: A total of 102 children were assessed. At 6 months old, 22.5 % had a developmental risk/delay. The prevalence of unexpected results for age in psychomotor development and sensorimotor intelligence was 13.7 % and 16.7 %, respectively. These children received early stimulation and all showed improved results. At 9 months old, the prevalence of risk/delay was 20.6 %: 14.7 % in psychomotor development and 9.8 % in sensorimotor intelligence. Conclusion: At 6 months old, 22.5 % of infants had a developmental risk/delay; at 9 months old, this prevalence was 20.6 %. An early assessment of development helped to detect disorders and provide an early intervention.

Assessment of sensorimotor intelligence and psychomotor development in clinically healthy infants assisted in the public health sector. / Evaluación de la inteligencia sensoriomotriz y del desarrollo psicomotor en lactantes clínicamente sanos asistidos en el sector público de salud.

INTRODUCTION: Infancy is the period of greatest neuroplasticity. The early detection of developmental disorders helps to provide a timely intervention aimed at reducing sequelae and/or complications. OBJECTIVE: To assess psychomotor and cognitive development in infants between 6 and 9 months old assisted in the public health sector. POPULATION AND METHODS: Descriptive study with follow-up of full-term infants who were clinically healthy at the time of assessment and had an adequate weight, height, and head circumference for their age. The Assessment Scale of Psychomotor Development and the Argentine Scale of Sensorimotor Intelligence were used in infants aged 6-9 months. Early stimulation was the intervention provided to those who showed a risk or delay. The prevalence of risk/delay of psychomotor development and sensorimotor intelligence was determined. The statistical software package R, version 3.3.1 was used. RESULTS: A total of 102 children were assessed. At 6 months old, 22.5 % had a developmental risk/delay. The prevalence of unexpected results for age in psychomotor development and sensorimotor intelligence was 13.7 % and 16.7 %, respectively. These children received early stimulation and all showed improved results. At 9 months old, the prevalence of risk/delay was 20.6 %: 14.7 % in psychomotor development and 9.8 % in sensorimotor intelligence. CONCLUSIONS: At 6 months old, 22.5 % of infants had a developmental risk/delay; at 9 months old, this prevalence was 20.6 %. An early assessment of development helped to detect disorders and provide an early intervention.

Introducción: La primera infancia es el período de mayor neuroplasticidad. La detección temprana de alteraciones del desarrollo permite la intervención oportuna para reducir secuelas y/o complicaciones. Objetivo: Evaluar el desarrollo psicomotor y cognitivo en lactantes asistidos en el sector público de salud, entre los 6 y 9 meses de edad. Población y métodos: Estudio descriptivo de seguimiento, de niños nacidos a término, clínicamente sanos en la evaluación, con peso, talla y perímetro cefálico adecuados para la edad. Se utilizó la Escala de Evaluación del Desarrollo Psicomotor y la Escala Argentina de Inteligencia Sensorio-motriz entre los 6 y 9 meses. En los casos en que se detectaron riesgo o retraso, se intervino con estimulación temprana. Se determinaron las prevalencias de riesgo/retraso del desarrollo psicomotor e inteligencia sensorio-motriz. Se utilizó el paquete estadístico R, versión 3.3.1. Resultados: Fueron evaluados 102 niños. A los 6 meses, el 22,5 % presentó riesgo/retraso en el desarrollo. Las prevalencias de resultados no esperados para la edad en desarrollo psicomotor y en inteligencia sensorio motriz fueron del 13,7 % y del 16,7 %, respectivamente. Estos niños recibieron estimulación temprana y todos mejoraron sus resultados. A los 9 meses, la prevalencia de riesgo/retraso fue del 20,6 %: el 14,7 % en desarrollo psicomotor y el 9,8 % en inteligencia sensorio motriz. Conclusión: El 22,5 % de los lactantes presentó riesgo/retraso del desarrollo a los 6 meses; la prevalencia a los 9 meses fue del 20,6 %. La evaluación temprana del desarrollo permitió detectar alteraciones e intervenir tempranamente.

Niveles de plombemia en niños de 1 a 6 años en la región de La Plata, Argentina: Identificación de factores de riesgo de exposición al plomo / Blood lead levels in children aged between 1 and 6 years old in La Plata, Argentina: Identification of risk factors for lead exposure

Introducción. El plomo es neurotóxico para los niños, aun en muy baja concentración sanguínea. Los factores de riesgo (FR) de exposición al plomo no están debidamente identificados en la región de La Plata. Los objetivos fueron determinar la media de plombemia e identificar los FR en niños de 1 a 6 años de la ciudad de La Plata y alrededores. Población y métodos. Se realizó un estudio de corte transversal en niños que concurrieron a controles de salud en centros de atención primaria. Se determinó la plombemia por absorción atómica y se realizó una encuesta socioambiental para relevar los FR. Se usó la prueba de Mann-Whitney para comparar medias. Se realizó un análisis estadístico multivariable para determinar los FR más relevantes. Resultados. Participaron 319 niños (51% de varones); la mediana y el rango intercuartílico de plombemia fue 2,2 (1,1-3,6) μg/dL. Se hallaron diferencias de media de plombemia significativas para edad ≤ 3 años, anemia, hábito de pica, hacinamiento, piso de tierra y escolaridad materna menor de 7 años. Los FR edad ≤ 3 años y hábito de pica tuvieron OR significativos. El OR ajustado por regresión logística fue significativo solo para edad ≤ 3 años. Conclusiones. La mediana de plombemia de la población estudiada fue de 2,2 μg/dL. Los principales FR de exposición al plomo identificados fueron edad ≤ 3 años y hábito de pica. Otros factores menos relevantes fueron anemia, escolaridad materna menor de 7 años, hacinamiento y piso de tierra.

Introduction. Lead has neurotoxic effects in children, even at a very low level in blood. The risk factors (RFs) for lead exposure have not been adequately identified in La Plata. The objectives of this study were to determine mean blood lead levels and identify RFs in children aged 1 to 6 years old living in La Plata and the outskirts. Population and methods. A cross-sectional study was conducted in children who attended primary health care centers for a health check-up. Blood lead levels were determined by atomic absorption spectroscopy, and a socioenvironmental survey was administered to outline RFs. The Mann-Whitney test was used to compare measurements. A multivariate statistical analysis was done to establish the most relevant RFs. Results. A total of 319 children participated (51% were boys); the median (interquartile range) blood lead level was 2.2 μg/dL (1.1-3.6 μg/dL). Significant mean differences in blood lead levels were observed for age ≤ 3 years old, anemia, pica behavior, overcrowding, dirt floors, and maternal education < 7 years. Age ≤ 3 years old and pica behavior were both RFs with significant odds ratios (ORs). The OR as adjusted by logistic regression was significant only for age ≤ 3 years old. Conclusions. The median blood lead level in the studied population was 2.2 μg/dL. The main RFs identified for lead exposure were age ≤ 3 years old and pica behavior. Other less relevant RFs included anemia, maternal education < 7 years, overcrowding, and dirt floors.

Blood lead levels in children aged between 1 and 6 years old in La Plata, Argentina. Identification of risk factors for lead exposure. / Niveles de plombemia en niños de 1 a 6 años en la región de La Plata, Argentina. Identificación de factores de riesgo de exposición al plomo.

INTRODUCTION: Lead has neurotoxic effects in children, even at a very low level in blood. The risk factors (RFs) for lead exposure have not been adequately identified in La Plata. The objectives of this study were to determine mean blood lead levels and identify RFs in children aged 1 to 6 years old living in La Plata and the outskirts. POPULATION AND METHODS: A cross-sectional study was conducted in children who attended primary health care centers for a health check-up. Blood lead levels were determined by atomic absorption spectroscopy, and a socioenvironmental survey was administered to outline RFs. The Mann-Whitney test was used to compare measurements. A multivariate statistical analysis was done to establish the most relevant RFs. RESULTS: A total of 319 children participated (51% were boys); the median (interquartile range) blood lead level was 2.2 pg/dL (1.1-3.6 pg/dL). Significant mean differences in blood lead levels were observed for age≤ 3years old, anemia, pica behavior, overcrowding, dirt floors, and maternal education < 7 years. Age≤ 3years old and pica behavior were both RFs with significant odds ratios (ORs). The OR as adjusted by logistic regression was significant only for age≤ 3years old. CONCLUSIONS: The median blood lead level in the studied population was 2.2 pg/dL. The main RFs identified for lead exposure were age≤ 3years old and pica behavior. Other less relevant RFs included anemia, maternal education < 7 years, overcrowding, and dirt floors.

INTRODUCCIÓN: El plomo es neurotóxico para los niños, aun en muy baja concentración sanguínea. Los factores de riesgo (FR) de exposición al plomo no están debidamente identificados en la región de La Plata. Los objetivos fueron determinar la media de plombemia e identificar los FR en niños de 1 a 6 años de la ciudad de La Plata y alrededores. POBLACIÓN Y MÉTODOS: Se realizó un estudio de corte transversal en niños que concurrieron a controles de salud en centros de atención primaria. Se determinó la plombemia por absorción atómica y se realizó una encuesta socioambiental para relevar los FR. Se usó la prueba de Mann-Whitney para comparar medias. Se realizó un análisis estadístico multivariable para determinar los FR más relevantes. RESULTADOS: Participaron 319 niños (51% de varones); la mediana y el rango intercuartílico de plombemia fue 2,2 (1,1-3,6) pg/dL. Se hallaron diferencias de media de plombemia significativas para edad ≤ 3 años, anemia, hábito de pica, hacinamiento, piso de tierra y escolaridad materna menor de 7 años. Los FR edad≤ 3años y hábito de pica tuvieron OR significativos. El OR ajustado por regresión logística fue significativo solo para edad ≤ 3 años. CONCLUSIONES: La mediana de plombemia de la población estudiada fue de 2,2 pg/dL. Los principales FR de exposición al plomo identificados fueron edad≤ 3años y hábito de pica. Otros factores menos relevantes fueron anemia, escolaridad materna menor de 7 años, hacinamiento y piso de tierra.

Red blood cell membrane fatty acid composition in infants fed formulas with different lipid profiles.

BACKGROUND: There is growing interest in the fatty acid composition of breast milk and substitute formulas used to replace or complement infant breastfeeding. AIM: The aims of this study were to assess the impact of two follow-up infant formulas based on cow milk fat, vegetable oils and different docosahexaenoic (DHA) and arachidonic (ARA) acid content on red blood cell membrane fatty acid composition, and determine the percent saturated fatty acid (SFA) incorporation into the membrane. STUDY DESIGN: This was a double-blind, randomized, controlled, parallel-group clinical trial. Infants received treatment or control product for at least four months before the age of six months. The control group (n=25) received standard infant formula (FA) and the treatment group (n=24) received the same formula supplemented with higher DHA and ARA content (FB). The reference group (n=47) consisted of normal healthy exclusively breastfed infants. OUTCOME MEASURE: Red blood cell membrane fatty acid composition was determined by capillary gas chromatography. RESULTS: Ninety-six infants completed the study (FA, 25; FB, 24; reference, 47). Higher DHA content reflected higher DHA percentage in the red blood cell membrane. Breast milk and FB did not show any significant differences in DHA content. ARA percentage was higher in breastfed infants and palmitic acid percentage was higher in FB- compared with FA-fed infants. CONCLUSION: DHA and palmitic acid percent distributions were higher in the red blood cell membrane of infants receiving FB. DHA percent distribution was not significantly different in FB-fed and breastfed infants. SFA percent distribution was not significantly different when comparing both formulas with breast milk.

Estudio cuali-cuantitativo del estado nutricional y la alimentación en niños de 1 a 3 años de familias de bajos recursos en dos grupos poblacionales con diferentes actividades productivas (Buenos Aires, Argentina), 2007-2008 / Quali-quantitative study of nutritional status and eating patterns in children aged 1-3 years from low-income families in two population groups with different productive activities (Buenos Aires, Argentina), 2007-2008

RESUMEN El objetivo de este artículo es describir el estado nutricional y los patrones de alimentación de niños de 1 a 3 años de familias de bajos recursos, que residen en dos áreas con diferentes actividades productivas: producción primaria y de bienes y servicios. Se realizó un estudio descriptivo de corte transversal, con metodología cuali-cuantitativa; se evaluó el estado nutricional antropométrico y bioquímico, ingesta alimentaria, características económicas y sociodemográficas, prácticas alimentarias y representaciones. Los resultados muestran que los niños del área de producción primaria presentaron menor prevalencia de anemia y deficiencia de hierro. Asimismo tuvieron un consumo superior de energía, calcio, zinc, vitamina A y proteínas y una mayor diversidad y calidad en el consumo de alimentos. Podemos concluir que el contexto geográfico de las familias que residen cerca de fuentes de producción primaria favorecería la interacción con individuos ajenos a la familia, ampliando la red social informal y el acceso a alimentos de mejor calidad nutricional.

ABSTRACT The aim of this article is to describe the nutritional status and eating patterns of children aged 1-3 years from low-income families who reside in areas with different productive activities: primary production and production of goods and services. A descriptive cross-sectional study was performed with a qualitative and quantitative methodology, evaluating anthropometric and biochemical nutritional status, food intake, economic and demographic characteristics, dietary practices and representations. The results show that children from areas of primary production had a lower prevalence of anemia and iron deficiency. They also had a higher consumption of energy, calcium, zinc, vitamin A and protein and a greater diversity and quality in food consumption. We can conclude that the geographical context of families closer to sources of primary production favors interaction with individuals outside of the family, expanding both the informal social network and access to better quality nutritional food.

[Quali-quantitative study of nutritional status and eating patterns in children aged 1-3 years from low-income families in two population groups with different productive activities (Buenos Aires, Argentina), 2007-2008]. / Estudio cuali-cuantitativo del estado nutricional y la alimentación en niños de 1 a 3 años de familias de bajos recursos en dos grupos poblacionales con diferentes actividades productivas (Buenos Aires, Argentina), 2007-2008.

The aim of this article is to describe the nutritional status and eating patterns of children aged 1-3 years from low-income families who reside in areas with different productive activities: primary production and production of goods and services. A descriptive cross-sectional study was performed with a qualitative and quantitative methodology, evaluating anthropometric and biochemical nutritional status, food intake, economic and demographic characteristics, dietary practices and representations. The results show that children from areas of primary production had a lower prevalence of anemia and iron deficiency. They also had a higher consumption of energy, calcium, zinc, vitamin A and protein and a greater diversity and quality in food consumption. We can conclude that the geographical context of families closer to sources of primary production favors interaction with individuals outside of the family, expanding both the informal social network and access to better quality nutritional food.

Diagnóstico del Estado Nutricional de Micronutrientes y Evaluación Antropométrica en una Población Infantil Suburbana de la Provincia de Buenos Aires / Diagnosis of Micronutrient Nutritional Status and Anthropometric in a Suburban Child Population in the Province of Buenos Aires

Objetivo: Determinar el estado nutricional evaluado por antropometría, y el estado nutricional de micro nutrientes en una población infantil.

Métodos: Se estudiaron 205 niños de 4 a 10 años de una comunidad suburbana de La Plata, Argentina. Se determino Peso/edad, Talla/edad y Peso/talla y se comparo las referencias. Para determinar las deficiencias de micro nutrientes y anemia se establecieron los siguientes puntos de cortes: Deficiencia de zinc y cobre < 70 mcg/dl (absorción atómica), hierro por ferritina < 12 ng/dl (quimioluminiscencia), hemoglobina < 11,5 g/dl (Coulter). Se realizó una encuesta social y de ingesta y de hábitos alimentarios. Los datos se analizaron con EpiInfo 6. Se utilizaron las correlaciones de Pearson y Spearman.

Resultados: La media de edad fue de 6,69 (± 2,04), 46,6 % de sexo femenino. El número de integrantes de familia fue de 6 (± 1,98), la dieta cubría las recomendaciones de proteínas, pero el 19,4 % no cubría las recomendaciones de calorías y 30,33 y 40 % de los niños no cubrieron las recomendaciones de hierro, zinc y vitamina A respectivamente. La prevalencia de insuficiente progresión de peso fue 4,5 % (Store Z < -2 de P/E) y por < Percentilo 10 de P/E 19,2 %; retraso crónico de crecimiento (<-2 score Z T/E) fue 5,6 %, y 23,2 % (< percentilo 10); por el indicador P/T la emaciación de primer grado alcanzó el 5%, sobrepeso y la obesidad llegó al 17,7 %. La prevalencia de anemia fue 21,6 %. El 6,8 % de los niños presento deficiencia de hierro, 5,1 % deficiencia de cobre y 11,3 % deficiencia de zinc. No se halló correlación significativa entre Peso/edad, peso/talla y Talla/ edad, con los niveles séricos de micro nutrientes y Hb, ni al comparar el estado nutricional y la prevalencia de deficiencia de cada micro nutrientes, excepto la prevalencia deficiencia de hierro en niños con retraso crónico de merecimiento que fue mayor que en el resto de los niños (p: 0,01).

Conclusión: La carencia de micro nutrientes afecta a todos los estados nutricionales estimados por antropometría en los niños estudiados. El sobrepeso y la obesidad aparecen como un problema nutricional emergente.

Diagnóstico del estado nutricional de micronutrientes y evaluación antropométrica en una población infantil suburbana de la Provincia de Buenos Aires / Diagnosis of micronutrient nutritional status and anthropometric in a suburban child population in the Province of Buenos Aires

Objetivo: Determinar el estado nutricional evaluado por antropometría, y el estado nutricional de micro nutrientes en una población infantil. Métodos: Se estudiaron 205 niños de 4 a 10 años de una comunidad suburbana de La Plata, Argentina. Se determino Peso/edad, Talla/edad y Peso/talla y se comparo las referencias. Para determinar las deficiencias de micro nutrientes y anemia se establecieron los siguientes puntos de cortes: Deficiencia de zinc y cobre < 70 mcg/dl (absorción atómica), hierro por ferritina < 12 ng/dl (quimioluminiscencia), hemoglobina < 11,5 g/dl (Coulter). Se realizó una encuesta social y de ingesta y de hábitos alimentarios. Los datos se analizaron con EpiInfo 6. Se utilizaron las correlaciones de Pearson y Spearman. Resultados: La media de edad fue de 6,69 (± 2,04), 46,6 % de sexo femenino. El número de integrantes de familia fue de 6 (± 1,98), la dieta cubría las recomendaciones de proteínas, pero el 19,4 % no cubría las recomendaciones de calorías y 30,33 y 40 % de los niños no cubrieron las recomendaciones de hierro, zinc y vitamina A respectivamente. La prevalencia de insuficiente progresión de peso fue 4,5 % (Store Z < -2 de P/E) y por < Percentilo 10 de P/E 19,2 %; retraso crónico de crecimiento (<-2 score Z T/E) fue 5,6 %, y 23,2 % (< percentilo 10); por el indicador P/T la emaciación de primer grado alcanzó el 5%, sobrepeso y la obesidad llegó al 17,7 %. La prevalencia de anemia fue 21,6 %. El 6,8 % de los niños presento deficiencia de hierro, 5,1 % deficiencia de cobre y 11,3 % deficiencia de zinc. No se halló correlación significativa entre Peso/edad, peso/talla y Talla/ edad, con los niveles séricos de micro nutrientes y Hb, ni al comparar el estado nutricional y la prevalencia de deficiencia de cada micro nutrientes, excepto la prevalencia deficiencia de hierro en niños con retraso crónico de merecimiento que fue mayor que en el resto de los niños (p: 0,01)...