Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae) / Alteração das células do intestino médio em besouros irradiados com radiação gama (Blaps polycresta, Coleoptera: Tenebrionidae)

Abstract This study was conducted to examine the effect of gamma radiation on biological specimens. Thus, our concept is to clarify that exposure to accumulated dose of 0.2 Gy gamma rays (0.66 rad/Sec. dose rate) from Cs137 source induces cellular perturbations in the midgut epithelium of the F1 progeny of Blaps polycresta, therefore affecting nutrition and growth. Beetles were reared in laboratory conditions and the newly emerged adults were irradiated with the aforementioned dose. Histological and ultrastructure anomalies of midgut cells (digestive and regenerative cells) were observed by 72 h after radiation exposure to ensure that the cells will not return to control state. Retardation in the development of the F1 progeny was also noticed and beetles died through two weeks. In the light of these observations, biological tissue act as an indicator to the continuous exposure to environmental radiation.

Resumo Este estudo foi conduzido para examinar o efeito da radiação gama em espécimes biológicos. Assim, nosso conceito é esclarecer que a exposição à dose acumulada de raios gama de 0,2 Gy (0,66 rad / seg. Dose) da fonte Cs137 induz perturbações celulares no epitélio do intestino médio da progênie F1 de Polycresta blaps, afetando a nutrição e crescimento. Besouros foram criados em condições de laboratório, e os adultos recém-emergidos foram irradiados com a dose acima mencionada. Anomalias histológicas e ultraestruturais das células do intestino médio (células digestivas e regenerativas) foram observadas 72 horas após a exposição à radiação, para garantir que as células não retornariam ao estado de controle. Retardo no desenvolvimento da progênie F1 também foi notado, e besouros morreram por duas semanas. À luz dessas observações, os tecidos biológicos atuam como um indicador para a exposição contínua à radiação ambiental.

Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae).

This study was conducted to examine the effect of gamma radiation on biological specimens. Thus, our concept is to clarify that exposure to accumulated dose of 0.2 Gy gamma rays (0.66 rad/Sec. dose rate) from Cs137 source induces cellular perturbations in the midgut epithelium of the F1 progeny of Blaps polycresta, therefore affecting nutrition and growth. Beetles were reared in laboratory conditions and the newly emerged adults were irradiated with the aforementioned dose. Histological and ultrastructure anomalies of midgut cells (digestive and regenerative cells) were observed by 72 h after radiation exposure to ensure that the cells will not return to control state. Retardation in the development of the F1 progeny was also noticed and beetles died through two weeks. In the light of these observations, biological tissue act as an indicator to the continuous exposure to environmental radiation.

Midgut cells alteration in gamma-irradiated beetles (Blaps polycresta, Coleoptera: Tenebrionidae)

Abstract This study was conducted to examine the effect of gamma radiation on biological specimens. Thus, our concept is to clarify that exposure to accumulated dose of 0.2 Gy gamma rays (0.66 rad/Sec. dose rate) from Cs137 source induces cellular perturbations in the midgut epithelium of the F1 progeny of Blaps polycresta, therefore affecting nutrition and growth. Beetles were reared in laboratory conditions and the newly emerged adults were irradiated with the aforementioned dose. Histological and ultrastructure anomalies of midgut cells (digestive and regenerative cells) were observed by 72 h after radiation exposure to ensure that the cells will not return to control state. Retardation in the development of the F1 progeny was also noticed and beetles died through two weeks. In the light of these observations, biological tissue act as an indicator to the continuous exposure to environmental radiation.

Resumo Este estudo foi conduzido para examinar o efeito da radiação gama em espécimes biológicos. Assim, nosso conceito é esclarecer que a exposição à dose acumulada de raios gama de 0,2 Gy (0,66 rad / seg. Dose) da fonte Cs137 induz perturbações celulares no epitélio do intestino médio da progênie F1 de Polycresta blaps, afetando a nutrição e crescimento. Besouros foram criados em condições de laboratório, e os adultos recém-emergidos foram irradiados com a dose acima mencionada. Anomalias histológicas e ultraestruturais das células do intestino médio (células digestivas e regenerativas) foram observadas 72 horas após a exposição à radiação, para garantir que as células não retornariam ao estado de controle. Retardo no desenvolvimento da progênie F1 também foi notado, e besouros morreram por duas semanas. À luz dessas observações, os tecidos biológicos atuam como um indicador para a exposição contínua à radiação ambiental.

Persisting role of healthcare settings in hepatitis C transmission in Pakistan: cause for concern.

Transmission of hepatitis C (HCV) in Pakistan is a continuing public health problem; 15 years ago it was linked to the practice of reusing therapeutic instruments in healthcare settings. We sought to examine current risk factors for HCV transmission in a hospital population in Karachi, Pakistan. We enrolled 300 laboratory-confirmed HCV-positive participants and 300 laboratory confirmed HCV-negative participants from clinics at Indus Hospital. Independent and significant risk factors for both men and women were: receiving o12 injections in the past year, blood transfusions, having had dental work performed, and delivery in hospital or transfusion for women. Interestingly, being of Mohajir origin or born in Sindh province were protective.Encouragingly, a strong protective effect was observed for those that reported bringing their own needle for injections (59%). The widespread reuse of therapeutic needles in healthcare settings in Karachi remains a major driver of the HCV epidemic.

Prospective surveillance study of the management of intussusception in UK and Irish infants.

BACKGROUND: Intussusception is the most common cause of acute intestinal obstruction in infants. This study examined the clinical presentation, management and outcomes of intussusception in this age group. METHODS: Prospective surveillance of intussusception in infants was carried out between March 2008 and March 2009 in the UK and Ireland. Monthly cards were sent to paediatric clinicians who were requested to notify cases of intussusception. RESULTS: The study identified 261 confirmed cases. The commonest presenting symptom/sign was non-bilious vomiting, in 210 (80·5 per cent) of the infants. Abdominal ultrasonography was done in 247 infants (94·6 per cent) and was diagnostic in 242 (98·0 per cent), compared with plain abdominal X-ray, which was diagnostic in 33 (23·6 per cent) of 140 infants. Enema reduction was carried out in 240 (92·0 per cent) of the 261 infants; the majority (237, 98·8 per cent) had pneumatic reduction with a success rate of 61·2 per cent (145 of 237). Surgery was required in 111 infants (42·5 per cent); 92 operations were as a result of unsuccessful enema reduction, and the remaining 19 infants (17·1 per cent) had primary surgery. Forty-four infants (39·6 per cent of operations) needed a bowel resection. The majority of children (238, 91·2 per cent) recovered uneventfully; 21 (8·0 per cent) had sequelae, one child died (0·4 per cent), and the outcome was unknown for one infant. CONCLUSION: This study described current treatment patterns for intussusception in infancy; these represent a benchmark for improved standards of care for this condition.

Management of superficial neck abscesses.

OBJECTIVE: To evaluate clinical features, management options and outcomes in children with neck abscesses, with a view to correlating this data with the different causative micro-organisms, specifically mycobacteria. PATIENTS AND METHODS: A retrospective chart review of fifty-one consecutive children who were admitted with suspected superficial neck abscesses to the Unit between January 1994 and June 1999 was performed. RESULTS: The causative organisms were identified in 21 cases--bacteria in 13 patients, atypical mycobacteria in 6 cases, and mycobacterium tuberculosis in 2 instances. Children with atypical mycobacterial infection had a significantly longer duration of symptoms at the time of admission (mean = 87 days) versus those with bacterial infection (mean = 6 days). Ultrasonography was performed in 20 patients in this series, and was seen to be of value in demonstrating a collection in situations where there was doubt on clinical basis alone. The outcome was seen to be complicated in all children with mycobacterial infections--either atypical or tuberculous. CONCLUSIONS: Neck abscesses are commonly encountered in paediatric practice. In most instances the diagnosis and treatment is straightforward, with an uncomplicated outcome. However, it is important to bear in mind that there exists a subset of abscesses caused by atypical mycobacteria, with greater risk of complications and recurrence, that needs special attention at the time of diagnosis, intervention and follow-up.

Prevalence of and factors associated with anxiety and depression among women in a lower middle class semi-urban community of Karachi, Pakistan.

OBJECTIVE: To study the prevalence of, and factors associated with anxiety and depression among women. DESIGN: A cross sectional survey. SETTING: A lower middle class semi-urban community of Karachi, Pakistan. PARTICIPANTS: A total of 1218 women between the ages of 18-50 years. METHODOLOGY: Systematically every third household was identified from which a woman was randomly selected. The Aga Khan University Anxiety and Depression Scale and a socio-demographic questionnaire were administered verbally by trained interviewers for assessing the prevalence of, and associated factors for anxiety and depression. RESULTS: A prevalence of 30% was found. Increasing age, lack of education and verbal abuse were the associated factors found to have an independent relationship. CONCLUSION: Providing education and reducing domestic abuse could lead to decrease in the prevalence of anxiety and depression in women.

Button battery ingestion: hazards of esophageal impaction.

Ingestion of button batteries has been seen with increasing frequency over the past decade. In a small number of reported cases, their impaction in the esophagus has led to serious, sometimes fatal, complications. The management of these cases has varied from expectant, supportive therapy to early surgical intervention. The authors report 2 pediatric patients in whom esophageal perforation developed after impaction of a disc battery. Both were treated conservatively with successful outcomes.

Respiratory distress in a child caused by lipoma of the esophagus.

A 6-year-old girl with a 2-year history of respiratory distress is described in this report. On investigation, a mass occupying the proximal half of the esophageal lumen, which was causing compression of the mediastinum, was found. It was removed surgically by a thoracic approach. On histological examination it was confirmed to be a lipoma of the esophagus. The child had an uneventful postoperative course. English-language literature on lipoma of the esophagus is reviewed briefly.

Tracheobronchial foreign bodies in children: reaching a diagnosis.

Tracheobronchial foreign body aspiration constitutes a significant cause of morbidity and mortality in the paediatric age group. Diagnosis is often delayed with symptoms and signs being attributed to other causes of respiratory distress. In this study we evaluate the importance of three criteria on which the diagnosis of foreign body aspiration may be based--a history of aspiration, physical examination and a plain chest radiograph. These parameters are correlated with findings on bronchoscopy. It is seen that a history of foreign body aspiration is notoriously unreliable in children of all age groups (p > 0.1). Similarly, presence or absence of findings on physical examination suggestive of foreign body aspiration or its sequalae are not always indicative (p > 0.1). Chest radiograph may be normal or misleading. It is therefore concluded that this is a diagnosis based on the cumulative evidence provided by all three parameters in the clinical context. The hazards of betel nut intake, the object most commonly incriminated, are emphasized.