Polychromatic visible and infrared light (480-3400 nm) downregulates the growth of hepatoma MH22a in mice.

We studied the effect of polychromatic visible (380-750 nm) (VIS) and combined with the visible infrared (480-3400 nm) (VIS-IR) radiation on the growth of hepatoma in mice. In the first series of experiments on C3HA mice with subcutaneously transplanted syngeneic hepatoma MH22a it was shown 1.5-4 times inhibition of tumor volume after irradiation of tumor-bearing mice with VIS-infrared light at a dose 4.8 J/ cm2. Mice irradiation at doses of 9.6 J/cm2 and 38.4 J/cm2 had no effect on the rate of tumor growth. Exposition to VIS and IR-light in all doses we used an increase of the surviveness of animals in the 1.5 and 2 times respectively was observed. In a second series of experiments we investigated the effect VIS-IR radiation on tumor cells in vitro with subsequent inoculation to intact mice. After implantation in mice irradiated cells at a dose of 4.8 J/cm2 9.6 J/cm2 inhibition of tumor growth during the first 25 days at 3-12 times as compared to control and increased survival in mice 1.5-2 respectively was observed. The main results of this study consists in the fact that none of the doses used VIS and a IR-radiation has not been shown to stimulate tumor growth both in irradiated mice with tumors, and the irradiation of MH22a hepatoma cells under in vitro conditions prior to transplantation of intact mice. Furthermore it was detected dose range VIS-IR light (4.8-9.6 Joules/cm2) when the rate of growth of hepatoma MH22a decreased and increased surviveness of animals.

[Monocytes of human blood as a target of Streptococcus pyogenes components].

AIM: Study the effect of components of destroyed streptococci on human blood monocyte functions related to processes of trans-endothelial migration in vitro. MATERIALS AND METHODS: Mononuclear leukocytes, isolated from blood of healthy donors, endothelial cells of EA.hy 926 line and supernatant of ultrasound disintegrated Streptococcus pyogenes (DSS) were the objects of the study. Evaluation of adhesion and monocyte migration, level of expression of adhesion molecules and phosphokinases on monocytes was carried out by flow cytometry using monoclonal antibodies. Cytokine concentration was determined by using standard commercial test systems in enzyme immunoassay. RESULTS: Under the effect of DSS, expression of adhesion molecules CD162 and CD11b, as well as phospho-p38 MAPK changed, IL-6 and IL-8 secretion induction took place. DSS caused enhancement of migration and adhesive activity of monocytes, however, inhibited intensity of trans-endothelial migration. CONCLUSION: Products of destroyed streptococci have a multi-directional effect on human blood monocytes, that could be explained by the presence of components with varying biological activity in DSS.

[Effect of polychromatic visible light combined with infrared radiation on tumorigenicity of murine hepatoma cells and their sensitivity to lytic activity of natural killers].

Tumorigenicity of murine hepatoma cells (MH22a) and their sensitivity to lysis by natural killers (NKs) have been studied after exposure to polychromatic visible and infrared light (VIS-IR, 480-3400 nm, 40 mW/cm2), similar to the terrestrial solar spectrum without its minor UV component, in order to elucidate the involvement of this important environmental and physiotherapeutic factor in regulation of the anti-tumor defense system. The MH22 cells were in vitro exposed to VIS-IR light and their sensitivity to lytic activity of NKs was evaluated. We found that sensitivity of MH22a cells to lysis by NKs after exposure to VIS-IR light at a dose of 4.8 J/cm2 increased 1.5-2 times, while it did not change after exposure to a dose of 9.6 J/cm2 at all ratios (1 : 5-1 : 50) of the number of NKs (effectors) to that of hepatoma cells (targets). The increase in the sensitivity of hepatoma cells to NKs was accompanied by structural changes of cell surface: the capability of supramembraneous glycoproteins (glycocalix) to sorb the vital dye alcian blue (AB) was significantly lower as compared with the unexposed cells of control group. However, no changes in AB sorption was revealed in hepatoma cells exposed to the light at a dose 9.6 J/cm2. Tumorigenicity of photo-irradiated MH22a cells has been studied in the in vivo experiments. Light-exposed (4.8 and 9.6 J/cm2) and intact hepatoma cells were transplanted into syngenic mice C3HA. Tumor volumes 25 days after transplantation proved to be smaller after exposure to the light at both doses than in the control group (4-4.5 times and 2.5-4 times, respectively), which correlated with the increase in the sensitivity to lisys by NKs and decrease in the AB sorption only after light exposure at dose 4.8 J/cm2. Using the flow cytometry method we could show that VIS-IR light at the applied doses did not interfere with the distribution of hepatoma cells over the cycle phases and thus deceleration of the tumor growth was not associated with cytostatic effect of VIS-IR light. To evaluate effect of polychromatic light on the growth of the preformed tumors, the 5-day course of daily light exposures of tumor bearing mice C3HA was carried out in 10 days after subcutaneous transplantation of 2 x 10(5) cells of syngene hepatoma when the tumors had developed in 100% animals. Like in the case of transplantation of the light-exposed cells, irradiation of the tumor bearing mice at doses 4.8-9.6 J/cm2 resulted in deceleration of tumor growth (2.1-2.9 and 2.2 times respectively) for 4 weeks as compared with non-irradiated mice.

[The efficacy of polychromatic visible and infrared radiation used for the postoperative immunological rehabilitation of patients with breast cancer].

The immunological rehabilitation of the patients with oncological problems after the completion of standard anti-tumour therapy remains a topical problem in modern medicine. The up-to-date phototherapeutic methods find the increasingly wider application for the treatment of such patients including the use of monochromatic visible (VIS) and near infrared (nIR) radiation emitted from lasers and photodiodes. The objective of the present study was to substantiate the expediency of postoperative immune rehabilitation of the patients with breast cancer (BC) by means of irradiation of the body surface with polychromatic visible (pVIS) in combination with polychromatic infrared (pIR) light similar to the natural solar radiation without its minor UV component. The study included 19 patients with stage I--II BC at the mean age of 54.0 +/- 4.28 years having the infiltrative-ductal form of the tumour who had undergone mastectomy. These patients were randomly allocated to two groups, one given the standard course of postoperative rehabilitation (control), the other (study group) additionally treated with pVIS + pIR radiation applied to the lumbar-sacral region from days 1 to 7 after surgery. A Bioptron-2 phototherapeutic device, Switzerland, was used for the purpose (480-3400 nm, 40 mW/cm2, 12 J/cm2, with the light spot diameter of 15 cm). The modern standard immunological methods were employed. It was found that mastectomy induced changes of many characteristics of cellular and humoral immunity; many of them in different patients were oppositely directed. These changes were apparent within the first 7 days postoperatively. The course of phototherapy (PT) was shown to prevent the postoperative decrease in the counts of monocytes and natural killer (NK) cells, the total amount of CD3+ -T-lymphocytes (LPC), CD4+ -T-helpers, activated T-lymphocytes (CD3+ HLA-DR+ cells) and IgA levels as well as intracellular digestion rate of neutrophil-phagocyted bacteria. Moreover PT promoted faster normalization of postoperative leukocytosis and activation of cytotoxic CD8+ -T-LPC, reduced the elevated concentration of immune complexes in blood. Among the six tested cytokines, viz. IL-1beta, TNF-alpha, IL-6, IL-10, IFN-alpha, and IFN-gamma, only the latter two underwent significant elevation of their blood concentrations (IL-6 within 1 day) and IFN-gamma (within 7 days after mastectomy). The course of PT resulted in the decrease of their levels to the initial values. The follow-up of the treated patients during 4 years revealed neither recurrence of the disease nor the appearance of metastases.

[Proliferation and tumorigenity of the murine hepatoma cells irradiated with polychromatic visible and infrared light].

In experiments in vitro, the effect of the polychromatic visible (VIS) light combined with polychromatic infrared light (VIS-IR, 480-3400 nm) and the effect of the entire spectrum of VIS radiation (385-750 nm) on the viability and proliferative activity of the murine hepatoma cells MH22a. In experiments in vivo, changes of tumorigenic properties of cells MH22a have been studied after the same kinds of light exposure. It was shown that irradiation of the hepatoma cells with two kinds of polychromatic light at a wide range of doses (4.8-38.4 J/cm2) did not lead to an increase in the number of dead cells for 24-72 h of cultivation and did not cause retardation of the hepatoma cell proliferation. Moreover, VIS-IR light at a dose of 4.8 J/cm2 and VIS light at a dose 38.4 J/cm2 stimulated cell proliferation in 24 h. Proliferation index increased by 1.6 and 1.4 times, respective, and the time of the cell number doubling decreased as compared with control. Studying the tumorigenic properties of irradiated tumor cells showed that, for 30 days after transplantation, of hepatoma cells in 24 h after their irradiation with VIS-IR light at a dose of 4.8 J/cm2 to syngenic mice C3HA, the tumor volume reduced significantly (2.6-4 times) of all stages of observation. The incidence of tumor formation decreased, whereas the survival of the tumor-bearing mice did not change. Transplantation of cells irradiated with the same light at a dose of 9.6 J/cm2 did not lead to significant changes of the tumor volume, the tumor formation incidence, and animal survival. The main contribution to the antitumor effect of the VIS-IR light seems to be made by the VIS component, as transplantation of cells irradiated with VIS alone light at a dose of 38.4 J/cm2 also stimulating proliferation of hepatoma cells in vitro into mice resulted in a reduction of their tumorigenic properties. However, the IR component in the combined VIS-IR radiation enhanced the antitumor effect of the VIS light; as a result, this effect was manifested after use of doses 8 times lower (4.8 J/cm2) than in the case of the VIS light alone (38.4 J/cm2). Mechanisms of the decrease of tumorigenic properties of hepatoma cells after irradiation with polychromatic light ad doses stimulating their proliferation in vitro are studied.

[Survival among patients with prostate cancer after distant radiotherapy and low-intensity near-infrared phototherapy].

The paper discusses the effect of photography with near-infrared photography on survival of patients with prostate cancer (PC) after distant radio- (DRT) and hormonal therapy (January 2000 - June 2007). The control group (n=251) were treated with standard radio- and hormonal therapy alone. Besides, patients of the study group (n=308) received phototherapeutic treatment to prevent radiation-induced injuries of the urinary bladder and rectum. The groups were compared relatively to PC cell differentiation, DRT and hormonal treatment. Survival was evaluated (Caplan-Meyer); log-rank test was used to compare the results. Overall survival in the study group of patients with localized (T1-2N0 M0) and generalized (T1-4N0-1 M1) tumors did not differ from that of controls (p > or = 0.05). In patients with locally advanced tumors (T3-4N0-1 M0, T1-4N1 M0), significant differences in survival between controls and study group were recorded beginning from year 3 of the investigation: 5-year survival rates were 67% and 89%, respectively (p < or = 0.05).

[Proliferation of normal and tumor cells at the presence of serum from patients with breast cancer after phototherapy with visible and near infrared light].

Simultaneous low-intensity visible (VIS) and near infrared (nIR) irradiation from laser and non-laser sources was used for treatment of complications developing in cancer patients after surgical tumor resection, chemo- and radiation therapy. However, the question remains about the impact of this physiotherapeutic method on proliferative activity of the patients' tumor cells and cells involved in wound healing, fibroblasts (FB) and keratinocytes (KC). In this paper, we studied the effect blood serum obtained from the patients with breast cancer after the course of irradiation with visible and NI light (480--3400 nm, 95 % polarization, 40 mW/cm2, 12 J/cm2) in postoperative period on the proliferative activity of primary cultures of human FB and KC, and of several human tumor cell lines (BT-474, HBL-100, Hs578T and A431). Seven-day course of phototherapy increase proliferation of FB (as compared to the initial level) and KC (as compared to postoperative level) by 22 and 28 %, respectively. The tumor cells BT-474, Hs578T and A431 showed statistically significant decrease in proliferative activity compared with the preoperative (initial) level by 31.5, 8.97 and 6.47%, respectively, whereas the cells BT-474, HBL-100, Hs578T and A431 also reduced their proliferative activity by 32,16, 8.65 and 6.26%, respectively, as compared with postperative level. The results obtained demonstrate the safety of the phototherapy with the visible and NI light for BC patients in the postoperative period.

[Application of low-power visible and near infrared radiation in clinical oncology].

Although low-power visible (VIS) and near infrared (nIR) radiation emitted from lasers, photodiodes, and other sources does not cause neoplastic transformation of the tissue, these phototherapeutic techniques are looked at with a great deal of caution for fear of their stimulatory effect on tumour growth. This apprehension arises in the first place from the reports on the possibility that the proliferative activity of tumour cells may increase after their in vitro exposure to light. Much less is known that these phototherapeutic modalities have been successfully used for the prevention and management of complications developing after surgery, chemo- and radiotherapy. The objective of the present review is to summarize the results of applications of low-power visible and near infrared radiation for the treatment of patients with oncological diseases during the last 20-25 years. It should be emphasized that 2-4 year-long follow-up observations have not revealed any increase in the frequency of tumour recurrence and metastasis.

[Changes in cytokine content in the peripheral blood of volunteers after their exposure to polychromatic visible and infrared light].

Anti-inflammatory, immunomodulating and wound-healing effects of visible and infrared (IR) radiation from laser and non-laser sources are widely used in current medicine. However, the role of pro- and anti-inflammatory cytokines in development of these effects has been poorly studied. A randomized, placebo-controlled, double blind study was made. Using ELISA, the content of 10 cytokines was studied in the peripheral blood of volunteers after a single and four daily irradiations of the sacral area (D = 15 cm) with polychromatic visible + IR polarized light (480-3400 nm, 12 J/cm2). The phototherapeutic sessions were accompanied by four blood exfusions for the study (to a total volume of 80 ml). In the control (placebo) group, irradiation was imitated, and blood samples of the same volume were drawn at the same time intervals as in volunteers of the main group. A fast decrease in the level of pro-inflammatory cytokines was revealed as soon as in 0.5 h after the irradiation. This level was retained until the end of the phototherapeutic course. At the parameters exceeding the norm, the contents of TNF-alpha, IL-6 and IFN-gamma fell, on average, by 34, 12 and 1.5 times, respectively. By the end of the course, the levels of IFN-gamma and of IL-12 decreased by 5 and 15 times, respectively. A fast decrease (by two-fold) was also characteristic of normal values of IL-6. Neither IL-1beta, nor IL-2 were detected in blood plasma of the examined people both before and after the irradiation. In parallel with a decrease in the proinflammatory factor levels the amount of anti-inflammatory cytokines was found to rise: that of IL-10--by 2.7-3.5 times in 0.5 h and at later terms at the initially normal parameters, and that of TGF-beta1--by 1.4-1.5 times at the initially decreased level. The IL-4 content did not change. A characteristic feature of the light effect was a fast rise of IFN-gamma amount--by 3.3-4.0 times in individuals with its initially normal level, with no changes in IFN-alpha content. The above-reported regularities of the light effects were also recorded at a direct (in vitro) irradiation of the examined volunteers' blood, as well as on addition of irradiated blood to a 10-fold volume of non-irradiated autologous blood, i.e. at a modeling of mixing, of a small amount of transcutaneously photomodified blood with its main circulating volume in the vascular bed of an irradiated person. Such a similarity of effects in blood following its irradiation in vivo and in vitro enables us to associate the fast changes of the cytokine content in the entire volume of peripheral blood with the transcutaneous photomodification of its small amounts, and with a "transfer" of the light effects by photomodified blood to the whole pool of circulating blood.

[Modulation of proliferation of peripheral blood lymphocytes after irradiation of volunteers with polychromatic visible and infrared light]. / Moduliatsiia proliferatsii limfotsitov perifericheskoi krovi posle oblucheniia dobrovol'tsev polikhromaticheskim vidimym i infrakrasnym svetom.

Effects on human immune system of visible and infrared (IR) radiation, the dominating types of solar light on Earth, still remain poorly studied. In the present work, a small area of the volunteers' body surface was irradiated with polychromatic visible + IR polarized (VIP) light, whose spectral range is close to the natural one (400-3400 nm, 12 J/cm2), and spontaneous and phytohemagglutinin (PHA)-induced DNA syntheses were studied by radiometric method in lymphocytes (Lym) of peripheral blood. This Irradiation stimulated both spontaneous and PHA-induced DNA synthesis in Lym but only in volunteers with initially decreased parameters of synthesis (on average, by 2.5 and 2.7 times, respectively), which was recorded 24 h after irradiation of volunteers, and after a 72 h cultivation of separated mononuclears. In the parallel experiments, blood of each volunteer was irradiated in vitro. Besides, by modeling situation in vivo, when a small amount of transcutaneously photomodified blood contacts its much larger circulating volume, the irradiated and non-irradiated samples of autologous blood were mixed at a 1:10 volume ratio. In Lym with the initially decreased synthesis level, the spontaneous synthesis elevated by 2 and 3 times, respectively, whereas stimulation of PHA-synthesis was observed only after addition of the irradiated blood to the intact one (by 2.2 and 1.6 times, respectively). A high degree of positive correlation in changing the studied parameters is revealed in irradiation of blood in vivo and in vitro. This makes it possible to associate the light-stimulating effect on Lym of the entire circulating blood with transcutaneous photomodification of its small amounts, and with action of such blood on the rest of blood. A similarity in the direction and additivity of mitogenic effects of VIP light and PHA was revealed. The obtained data enable us to suggest that therapy employing polychromatic visible and IR light would promote presumably an increase in the number of Lym in peripheral blood and an enhancement of their response to antigenic stimulus.

[Enhancement of fibroblast growth promoting activity of human blood after its irradiation in vivo (transcutaneously) and in vitro with visible and infrared polarized light]. / Povyshenie rostostimuliruiushchei aktivnosti krovi cheloveka dlia fibroblastov posle ee oblucheniia in vivo (transkutanno) i in vitro vidimym i infrakrasnym poliarizovannym svetom.

Visible and infrared (IR) irradiation of laser and non-laser sources has a pronounced wound-healing effect promoting tissue repair without hyperproduction of connective tissue elements. This effect develops as a consequence of local and systemic light effects, but many aspects of their mechanism have been yet unclear. In the present work, we have shown that in 0.5 h after irradiation of a small area of the volunteers' body surface with polychromatic visible + IR light (400-3400 nm, 95% polarization, 12 J/cm2) the amounts of PDGF and TGF-beta 1 in the blood serum increase, on average, by 20 and 43%, respectively. This effect is preserved for at least 24 h to be recorded only in volunteers with the initially normal and decreased levels of the growth factors; the initially elevated content of PDGF-AB decreases. Addition of such a plasma (2.5%) to the nutrient medium of primary cultures of human embryonal fibroblasts stimulates cell proliferation, on average, by 10 and 17%, but only in the case if the initial growth-promoting (GP) blood activity was low. Similar changes occur in parallel experiments following irradiation of blood samples of the same volunteers in vitro, as well as at mixing irradiated and non-irradiated autologous blood at the ratio 1:10 (v/v), i.e. at modeling a situation in the vascular bed, when the transcutaneously photomodified blood contacts with the rest of its volume. Similar changes in the blood GP activity under conditions in vitro were recorded as well after 4-9 daily phototherapy sessions. This allows us to suggest that changes in GP activity of circulating blood of the irradiated volunteers may be, to a large extent, the consequence of effect exerted on the blood by small amounts of transcutaneously photomodified blood. The obtained results are discussed in terms of light effect on wound healing and scar tissue formation, with regard to the authors' previous data on much higher GP of the irradiated blood in respect to keratinocytes, the fast decrease in proinflammatory cytokine levels, and the increase in IFN-gamma content.

[Changes in the expression of membrane markers and in the number of human blood monocytes after single and repeated courses of visible and infrared light at therapeutic doses]. / Izmeneniia ékspressii membrannykh markerov i kolichestva mononuklearov krovi cheloveka posle ee oblucheniia in vivo i in vitro vidimym i infrakrasnym svetom v terapevticheskikh dozakh.

An attempt has been made to prove that the immunomodulating effect of therapeutic doses of polychromatic visible + infrared polarized (VIP) light at its application to a small body surface area is connected with a transcutaneous photomodification of a small amount of blood in superficial skin microvessels. For this purpose, in parallel experiments, using monoclonal antibodies, the membrane phenotype of circulating blood mononuclears was studied after irradiation of volunteers, of samples of their blood in vitvo, and of a mixture of the irradiated and non-irradiated autologous blood in a 1:10 volume ratio, thereby modeling events in vivo, when a small amount of the transcutaneously photomodified blood in the vascular bed contacts its main circulating volume. In this variant of experiment, a great similarity has been established of changes in expression of mononuclear membrane markers (CD3, CD4, CD8, CD20, CD16, HLA-DR and to a lesser degree of CD25); the ability has been proven of the photomodified blood to "translate" the light-induced changes to a much higher volume of non-irradiated blood, which might represent a mechanism of the systemic immunomodulating effect of phototherapy. Under conditions in vivo and in vitro, the most "reactive" were HLA-DR+, CD20+, CD16+, CD4+, and 0-cells. An increase of the total number of lymphocytes and monocytes has been shown by the end of the 10-day-long phototherapeutic course. The regulatory character of the single and course sessions of the VIP light on the blood immunocompetent cells is substantiated: depending on the initial state of the immune system, the VIP light can produce both stimulating and inhibitory effect on lymphoid cell subpopulations, which opens large possibilities of using this method for correction of immunological disturbances in diseases of different etiopathogenesis.

[Stimulation of DNA repair in human cells damaged by UV and ionizing radiation with soluble growth factors of photomodified blood]. / Stimuliatsiia reparatsii DNK rastvorimymi faktorami fotomodifitsirovannoi krovi v kletkakh cheloveka, povrezhdennykh UF- i ioniziruiushchei radiatsiei.

Exposure of a small skin area of volunteers to UV light in 1 minimal erythemal dose is accompanied by rapid appearance in the circulating blood of soluble factors able to restore proliferation of X-ray-damaged autologous lymphocytes, to decrease frequency of chromosome breaks, and to stimulate unscheduled DNA synthesis. The appearance of such an activity in the blood can be also induced without skin irradiation. For this, one volume of a directly UV-irradiated blood is to be mixed in vitro with 10-fold volumes of intact blood, thus modeling the in vivo situation, when a small amount of transcutaneously UV-irradiated blood mixes with intact blood in the circulation. It has been found that the platelet-derived growth factor and epidermal growth factor, added at physiological concentrations to the culture medium, decrease chromosome break frequency in X-damaged cells.

Molecular dosimetry by flow cytometric detection of thymine dimers in mononuclear cells from extracorporally UV-irradiated blood.

UV-induced DNA damage in mononuclear leucocytes can be quantified by flow cytometry of fluorescence from a labelled monoclonal antibody that specifically binds to thymine dimers (T<-->T): specific fluorescence is already detectable after exposures of 1-2 J m-2 of 254 nm radiation and shows a linear relationship with dose. The distribution of UV fluences over an irradiated volume can thus be ascertained by measuring the UV-induced T<-->T loads of the individual cells from that volume. After irradiation of mononuclear cells in a phosphate buffer solution in a Petri dish, most cells showed a similar intensity of specific T<-->T fluorescence, forming a single sharp peak in the fluorescence histogram. This signifies an even distribution of fluences over the cells. It was noticed, however, that a variable minor fraction of mononuclear cells (usually less than 10%) could be resistant to immunostaining; this fraction was rejected from the calculation of the specific fluorescence. The flow cytometric technique was also applied to blood cells exposed in an ISOLDA device, which is in use in Russian clinics for UV irradiation of whole blood for therapeutical purposes. Only a small fraction of mononuclear cells in a sample of whole blood treated in ISOLDA acquired a detectable T<-->T load after exposure to lamps which emit predominantly either UVC or UVB light ((3.6 +/- 1.0)% and (1.8 +/- 0.4)% of all analysed cells respectively). This small fraction had received a large variation in fluences, resulting in differences in nuclear T<-->T loads by a factor of 200.(ABSTRACT TRUNCATED AT 250 WORDS)

[Changes of immunosorption properties in the blood and its components at various times after UV-irradiation]. / Izmenenie immunosorbtsionnykh svoistv krovi i ee komponentov v raznye sroki posle UF-oblucheniia.

Immunosorption activity in reference to antirhesus antibodies, of UV-irradiated Rh-positive preserved blood as well as of red blood cell pack and leucocytic-thrombocytic suspension prepared from this blood, was studied in varying terms after irradiation. The whole blood and red blood cell pack immunosorption activity significantly increased immediately after irradiation. The effect lasted during two days. The sorption capacity of leucocytic-thrombocytic suspension remained unchanged immediately after irradiation, and two days later it was almost lost.

[Changes in the surface and activation of circulating leukocytes after autologous transfusion of UV-irradiated blood]. / Izmeneniia poverkhnosti i aktivatsii tsirkuliruiushchikh leikotsitov pri autotransfuziiakh UF-obluchennoi krovi.

Under study was the dynamics of functional properties of leukocytes of healthy people and patients after UV-irradiation of their blood, mixing the UV-irradiated and non-irradiated blood. It was shown that UV-irradiation and mixing the irradiated and non-irradiated blood facilitated the liberation of bactericidal cation proteins of leukocytes, higher expression of membrane receptors, growth factors, activation of reparation of DNA after its injury by gamma-rays. It proves the role of immediate activation of leukocytes of the circulating blood in trigger mechanisms of effects of autotransfusion of UV-irradiated blood.

[The effect of UV irradiation and of UV-irradiated autologous blood on the functional state of human peripheral blood lymphocytes]. / Vliianie UF-oblucheniia i UF-obluchennoi autologichnoi krovi na funktsional'noe sostoianie limfotsitov perifericheskoi krovi cheloveka.

The effect of UV irradiation (UVI, 254 nm) and of UV-irradiated autologous blood on the spontaneous and mitogen-induced DNA-synthetic activity of intact lymphocytes has been studied. Lymphocytes were isolated from nonirradiated and irradiated blood, and from the mixture of UV-irradiated blood with the intact one in the volume ratio close to that in the blood stream during UV-irradiated blood autotransfusion (1:10, 1:40, 1:160). It has been shown that UVI of the whole blood caused in some donors the increase in spontaneous DNA synthesis, while in others the decrease or no statistically significant changes were observed. The analysis of the results obtained shows an inverse relation of the UVI effect to the initial level of spontaneous DNA synthesis (r = -0.68). In contrast to direct UVI effect, an addition of UV-irradiated blood to the autologous intact one resulted in an increase in spontaneous DNA synthesis in lymphocytes of all the samples examined. A 7-day cocultivation of lymphocytes, isolated from irradiated and nonirradiated blood samples, revealed a 1.8 times increase compared to the calculated value. The mitogen-induced DNA synthesis has a low sensitivity to UV rays, since the mitogens and the irradiation of optical range have presumably the common targets. It is assumed that photomodification of HLA-D/DR antigens can be a trigger mechanism for activation of immunocompetent cells by UVI.