Rumpel-Leede phenomenon as a rare complication after transulnar percutaneous coronary angiography and intervention.

The Rumpel-Leede sign, characterized by a non-blanching petechial rash distal to venous occlusion, has historically been associated with thrombocytopenia and capillary fragility. This phenomenon has been observed in various situations involving pressure application, such as tourniquet tests and continuous non-invasive pressure monitoring. Here, we present a case of Rumpel-Leede sign occurring after transulnar percutaneous coronary angiography in a 55-year-old female patient with a history of myocardial infarction. The patient had an uneventful recovery, highlighting the benign nature of the rash and the lack of intervention required. This underscores the importance of recognizing this sign and its association with specific procedures.

Characterization and Optimization of Multiomic Single-Cell Epigenomic Profiling.

The snATAC + snRNA platform allows epigenomic profiling of open chromatin and gene expression with single-cell resolution. The most critical assay step is to isolate high-quality nuclei to proceed with droplet-base single nuclei isolation and barcoding. With the increasing popularity of multiomic profiling in various fields, there is a need for optimized and reliable nuclei isolation methods, mainly for human tissue samples. Herein we compared different nuclei isolation methods for cell suspensions, such as peripheral blood mononuclear cells (PBMC, n = 18) and a solid tumor type, ovarian cancer (OC, n = 18), derived from debulking surgery. Nuclei morphology and sequencing output parameters were used to evaluate the quality of preparation. Our results show that NP-40 detergent-based nuclei isolation yields better sequencing results than collagenase tissue dissociation for OC, significantly impacting cell type identification and analysis. Given the utility of applying such techniques to frozen samples, we also tested frozen preparation and digestion (n = 6). A paired comparison between frozen and fresh samples validated the quality of both specimens. Finally, we demonstrate the reproducibility of scRNA and snATAC + snRNA platform, by comparing the gene expression profiling of PBMC. Our results highlight how the choice of nuclei isolation methods is critical for obtaining quality data in multiomic assays. It also shows that the measurement of expression between scRNA and snRNA is comparable and effective for cell type identification.

Ten Epidemiological Parameters of COVID-19: Use of Rapid Literature Review to Inform Predictive Models During the Pandemic.

Objective: To describe the methods used in a rapid review of the literature and to present the main epidemiological parameters that describe the transmission of SARS-Cov-2 and the illness caused by this virus, coronavirus disease 2019 (COVID-19). Methods: This is a methodological protocol that enabled a rapid review of COVID-19 epidemiological parameters. Findings: The protocol consisted of the following steps: definition of scope; eligibility criteria; information sources; search strategies; selection of studies; and data extraction. Four reviewers and three supervisors conducted this review in 40 days. Of the 1,266 studies found, 65 were included, mostly observational and descriptive in content, indicating relative homogeneity as to the quality of the evidence. The variation in the basic reproduction number, between 0.48 and 14.8; and the median of the hospitalization period, between 7.5 and 20.5 days stand out as key findings. Conclusion: We identified and synthesized 10 epidemiological parameters that may support predictive models and other rapid reviews to inform modeling of this and other future public health emergencies.

ALK Negative Inflammatory Myofibroblastic Tumor Synchronous with Classical Hodgkin Lymphoma.

Inflammatory myofibroblastic tumor or inflammatory pseudotumor was first described by Brunn in 1932. Umiker et al. named it so in 1954 for its ability to mimic malignancy clinically and radiologically [Med Pediatr Oncol 2000; 35(5): 484-7]. This tumor, characterized by its rareness, affects mainly the lung and the orbit. Histologically, the tumor is characterized by the presence of fibroblasts, myofibroblasts, polymorphs, lymphocytes, and neutrophils. This case report and review of literature present a male patient, 37 years old, with a bulky mediastinal lesion at the topography of the 4th rib. Histopathology reveals Hodgkin's Lymphoma (HL) concomitant with inflammatory myofibroblastic tumor (IMT). This report aims to emphasize the relevance of differential diagnoses to a better medical assistance. To the best of our knowledge, a case with this characteristic has never been seen before.

Habilidad en la solución de casos clínicos, post intervención educativa con la enseñanza del proceso de enfermería / Skill in the solution of clinical cases, post educational intervention with the teaching of the nursing process / Habilidade na solução de casos clínicos, intervenção pós-educacional com o ensino do processo de enfermagem

En el presente estudio se llevó a cabo una intervención educativa basada en la solución de problemas, con la finalidad de valorar su impacto en el aprendizaje del proceso de Enfermería y en el desarrollo de habilidades del pensamiento crítico en alumnos del Sistema Universidad Abierta (SUA). Objetivo: Evaluar el efecto de una intervención educativa con la enseñanza del Proceso de Enfermería para la solución de casos clínicos de los estudiantes que ingresan a la Licenciatura en Enfermaría del SUA. Metodología: Esta investigación de corte evaluativos, se inserta en la línea educativa y se considera Correlacional y Cuasiexperimental, en el que se utilizó el diseño de cuatro grupos de Solomon. Resultados; Los estudiantes que participaron en el seminario de proceso Atención de Enferme´ria tuvieron mayor habilidad en la solución de casos clínicos. Conclusiones: Este estudio nos hace pensar en que la forma de nuestro actuar docente puede y debe favorecer que los alumnos desarrollen las habilidades intelectuales, cognitivas e interpersonales que le ayuden a pensar y a realizar juicios críticos. Se debe considerar que los alumnos del SUA son personas adultas que requieren de un cambio en la metodología de la relación docente, donde no se reproduzcan los esquemas tradicionales de enseñanza, sino que fomente una relación dialógica, en donde puedan rescatar las experiencias de los estudiantes.

In the present study, an educational intervention baasada was carried out in the solution of problems, with the purpose of assessing its impact on the learning of the Nursing process and on the development of critical thinking skills in students of the Open University System (SUA). Objective: To evaluate the effect of an educational intervention with the teaching of the Nursing Process for the solution of clinical cases of the students who enter the Bachelor in Nursing of the SUA. Methodology: This evaluative research is inserted in the educational line and is considered Correlational and Cuasiexperimental, in which the design of four Solomon groups was used. Results; The students who participated in the seminar on the process of Attention to Disease had greater ability in the solution of clinical cases. Conclusions: This study makes us think that the way our teaching act can and should help students develop intellectual, cognitive and interpersonal skills that help them think and make critical judgments. It should be considered that the students of the SUA are adults who require a change in the methodology of the teaching relationship, where the traditional teaching schemes are not reproduced, but that foster a dialogical relationship, where they can rescue the students' experiences .

No presente estudo, uma intervenção educativa baasada foi realizada na solução de problemas, com o objetivo de avaliar seu impacto na aprendizagem do processo de enfermagem e no desenvolvimento de habilidades de pensamento crítico em estudantes do Sistema Universitário Aberto (SUA). Objetivo: Avaliar o efeito de uma intervenção educacional com o ensino do Processo de Enfermagem para a solução de casos clínicos dos alunos que inserem o Bacharel em Enfermagem da SUA. Metodologia: Esta pesquisa avaliadora é inserida na linha educacional e é considerada Correlacional e Cuasiexperimental, na qual o projeto de quatro grupos Salomão foi utilizado. Resultados; Os alunos que participaram do seminário sobre o processo de atenção à doença apresentaram maior capacidade na solução de casos clínicos. Conclusões: Este estudo nos faz pensar que a forma como o nosso ato de ensino pode e deve ajudar os alunos a desenvolver habilidades intelectuais, cognitivas e interpessoais que os ajudem a pensar e fazer julgamentos críticos. Deve-se considerar que os alunos do SUA são adultos que exigem uma mudança na metodologia da relação de ensino, onde os esquemas de ensino tradicionais não são reproduzidos, mas que promovam uma relação dialógica, onde podem resgatar as experiências dos alunos .