ABSTRACT
The regulation of protein kinase B (AKT) phosphorylation by Tripartite motif-containing protein 31 (TRIM31) is implicated as an essential mechanism in the progression of many malignant tumors. Nevertheless, the function of the TRIM31/AKT pathway in oral squamous cell carcinoma (OSCC) remains elusive. Here, immunohistochemistry analysis of human OSCC tissue microarrays indicated significantly higher levels of TRIM31 and phosphorylated AKT (p-AKT) in OSCC tumors than in adjacent tissue samples. Also, we detected a positive association between TRIM31 expression and clinical OSCC development. In in vitro studies, TRIM31 knockdown severely impaired OSCC cell growth, invasion, and migration. By contrast, TRIM31 overexpression improved these cell behaviors, while subsequent AKT inhibition abrogated the effect. In vivo tumorigenesis experiments using nude mice also validated the effects of TRIM31/AKT signaling in tumor growth. Furthermore, TRIM31 upregulation facilitated glucose uptake, as well as lactate and adenosine triphosphate production of OSCC cells, while such positive effects on glycolysis and malignant cell phenotypes were reversed by treatment with AKT or glycolysis inhibitors. In conclusion, TRIM31 may improve OSCC progression by enhancing AKT phosphorylation and subsequent glycolysis. Hence, TRIM31 has the potential as a treatment target in OSCC.
Subject(s)
Carcinoma, Squamous Cell , Head and Neck Neoplasms , Mouth Neoplasms , Animals , Humans , Mice , Carcinoma, Squamous Cell/genetics , Cell Line, Tumor , Cell Movement/genetics , Cell Proliferation/genetics , Glycolysis , Mice, Nude , Mouth Neoplasms/genetics , Phosphorylation , Proto-Oncogene Proteins c-akt/metabolism , Squamous Cell Carcinoma of Head and Neck , Tripartite Motif Proteins/metabolism , Ubiquitin-Protein Ligases/geneticsABSTRACT
We analyze the effect of decoherence, modeled by local quantum channels, on quantum critical states and we find universal properties of the resulting mixed state's entanglement, both between system and environment and within the system. Renyi entropies exhibit volume law scaling with a subleading constant governed by a "g function" in conformal field theory, allowing us to define a notion of renormalization group (RG) flow (or "phase transitions") between quantum channels. We also find that the entropy of a subsystem in the decohered state has a subleading logarithmic scaling with subsystem size, and we relate it to correlation functions of boundary condition changing operators in the conformal field theory. Finally, we find that the subsystem entanglement negativity, a measure of quantum correlations within mixed states, can exhibit log scaling or area law based on the RG flow. When the channel corresponds to a marginal perturbation, the coefficient of the log scaling can change continuously with decoherence strength. We illustrate all these possibilities for the critical ground state of the transverse-field Ising model, in which we identify four RG fixed points of dephasing channels and verify the RG flow numerically. Our results are relevant to quantum critical states realized on noisy quantum simulators, in which our predicted entanglement scaling can be probed via shadow tomography methods.
ABSTRACT
Two Gram-stain negative aerobic bacterial strains were isolated from the bark tissue of Populus × euramericana. The novel isolates were investigated using a polyphasic approach including 16S rRNA gene sequencing, genome sequencing, average nucleotide identity (ANI) and both phenotypic and chemotaxonomic assays. The genome core gene sequence and 16S rRNA gene phylogenies suggest that the novel isolates are different from the genera Snodgrassella and Stenoxybacter. Additionally, the ANI, G+C content, main fatty acids and phospholipid profile data supported the distinctiveness of the novel strain from genus Snodgrassella. Therefore, based on the data presented, the strains constitute a novel species of a novel genus within the family Neisseriaceae, for which the name Populibacter corticis gen. nov., sp. nov. is proposed. The type strain is 15-3-5T (= CFCC 13594T = KCTC 42251T).
