ABSTRACT
During an island-wide PCR-based surveillance study of beta-lactam resistance in multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter calcoaceticus-baumannii complex isolates obtained from 17 different hospitals, 10 KPC-positive Acinetobacter isolates were identified. DNA sequencing of the bla(KPC) gene identified KPC-2, -3, and -4 and a novel variant, KPC-10. This is the first report of a KPC-type beta-lactamase identified in Acinetobacter species.
Subject(s)
Acinetobacter Infections/epidemiology , Acinetobacter/classification , Acinetobacter/drug effects , beta-Lactam Resistance/genetics , beta-Lactamases/genetics , Acinetobacter/enzymology , Acinetobacter/isolation & purification , Acinetobacter Infections/microbiology , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Microbial Sensitivity Tests , Middle Aged , Population Surveillance/methods , Puerto Rico/epidemiologyABSTRACT
During a 6-month period, 37/513 (7.2%) Pseudomonas aeruginosa isolates belonging to 13 pulsed-field gel electrophoresis (PFGE) groups from Puerto Rican hospitals were carbapenem nonsusceptible. Seven of 37 isolates from four PFGE groups carried bla(IMP-18), and 25/37 isolates from seven PFGE groups carried bla(KPC). The results indicated the clonal spread of bla(KPC)-positive P. aeruginosa isolates into several Puerto Rican hospitals and the dissemination of bla(IMP-18) and bla(KPC) into genetically unrelated isolates.
