Anesthetic efficacy of ketamine-diazepam, ketamine-xylazine, and ketamine-acepromazine in Caspian Pond turtles (Mauremys caspica).

OBJECTIVES: The objective of this study was to assess the efficacy of different anesthetic drug combinations on the Caspian Pond turtles (Mauremys caspica). SUBJECTS AND METHODS: Three groups of the Caspian Pond turtles (n = 6) were anesthetized with three different drug combinations. Initially, a pilot study was conducted to determine the best drug doses for the anesthetization of the turtles, and according to these results, ketamine-diazepam (120 mg/kg ketamine hydrochloride [5%] and 2 mg/kg diazepam [5%]), ketamine-acepromazine (120 mg/kg ketamine hydrochloride [5%] and 1 mg/kg acepromazine [1%]), and ketamine-xylazine (120 mg/kg ketamine hydrochloride [5%] and 1 mg/kg xylazine [2%]) were injected intramuscularly. The onset times of anesthetization and the recovery time were measured. Statistical analysis of the data was performed using one-way analysis of variance followed by t-tests, and P < 0.05 was considered statistically significant. RESULTS: There were statistically significant differences in the mean of the onset times of anesthesia and recovery time among the three drug combinations depending on the treatment used. The onset of anesthesia of the animals treated with the ketamine-diazepam combination was 60% and 42% shorter, for male and female turtles, respectively, compared to that obtained with the ketamine-acepromazine combination and 64% (male turtles) and 50% (female turtles) shorter than that obtained with the ketamine-xylazine combination. Further, the recovery time, in male turtles, was 17% shorter in animals treated with the first drug combination than those treated with the ketamine-acepromazine combination and 37% shorter than those treated with the ketamine-xylazine combination. The recovery time, in female turtles, did not seem to be significantly different among treatments. CONCLUSIONS: The study showed that the ketamine-diazepam drug combination is the anesthetic combination with the fastest onset time and shortest recovery time.

Mercury, lead, and cadmium in tissues of the Caspian Pond Turtle (Mauremys caspica) from the southern basin of Caspian Sea.

Concentrations of cadmium, lead, and mercury were measured in different tissues (liver, muscle, and shell) of 60 Caspian Pond Turtles collected from Tajan and Shiroud Rivers, southern basin of the Caspian Sea. Based on the results, different tissues showed different capacities for accumulating trace elements. The general trend of metals accumulation was: liver > shell > muscle. Results also showed that accumulation of these elements was not significantly different between sex and river in turtles (p > 0.05). Based on the results, Hg and Pb concentrations recorded in the present study were higher than some of the maximum concentration permissible. To our knowledge, this is the first report into heavy metal accumulation in tissues and organs of Caspian Pond Turtle from the southern basin of Caspian Sea. Further studies are needed to measure different heavy metals and trace metals in this valuable species.

A comparative study of trace metals in male and female Caspian kutum (Rutilus frisii kutum) from the southern basin of Caspian Sea.

This study focuses on the extent of zinc (Zn), copper (Cu), cadmium (Cd), cobalt (Co), manganese (Mn), lead (Pb), mercury (Hg), and arsenic (As) bioaccumulation in edible muscles of Caspian kutum (Rutilus frisii kutum), in both male and female sexes at Noor and Babolsar coastal regions from the southern basin of Caspian Sea. These values were compared with the WHO and the UNFAO safety standards regarding the amount of the abovementioned heavy metals in fish tissues (mg/kg ww). Results showed that the accumulation of these elements (except for Zn) was not significantly different between sexes of male and female in Babolsar coastal regions (P > 0.05). In the other hand, accumulation of Hg and As at edible muscles of Caspian kutum has significant difference between two sexes of male and female in Noor coastal regions (P < 0.05), the female had higher concentration than the male. Furthermore, it was not significantly correlated with sex and rivers in length and weight of fish (R 2 < 0.50; P > 0.05). Based on the results, the concentration of heavy metals in the studied fish tissues proved to be significantly lower than international standards (P < 0.05), so its consumption is not a threat to the health of consumers.

Variations in nutrient concentration and phytoplankton composition at the euphotic and aphotic layers in the Iranian coastal waters of the Southern Caspian Sea.

Temporal variations and regional distributions of dissolved nutrients and their elemental ratios in the Iranian coastal waters of the Southern Caspian Sea were investigated. The data were collected in 1996-97 (Phase I, as a background data and undisturbed ecosystem) and in 2005 (Phase II, as a disturbed ecosystem) at sampling points (from 10 to 100 m depths). In addition to the two main sampling exercises, additional sample collections were carried out during the period of 1994 to 2004 as a long-term study. This study showed that the dissolved inorganic nitrogen/dissolved inorganic phosphorus (DIN/DIP) ratios in the southern Caspian Sea vary within a very narrow range (4.47 to 5.78) within the euphotic and aphotic layers and is by one order of magnitude lower than what have been reported for several other marine ecosystems. Phytoplankton growth seems to be nitrogen limited while the levels of P and Si always remain high. Factor Analysis/Principal Component Analysis (FA/PCA) of the correlation matrix showed that the nitrogen compounds are associated with the main factor accounting for 25.7-26.2% of the total variance for both the sampling periods. During Phase I, the Chrysophyta were the major group, whereas during Phase II the proportion of Chrysophyta in the total community progressively decreased, while the other groups increased.