Resonance Raman study of oxoiron(IV) porphyrin π-cation radical complex: Porphyrin ligand effect on ν(Fe=O) frequency.

Resonance Raman (rR) spectroscopy has been applied to study the nature of the iron-oxo (Fe=O) moiety of oxoiron(IV) porphyrin π-cation radical complex (CompI). While the axial ligand effect on the nature of the Fe=O moiety has been studied with rR spectroscopy, the porphyrin ligand effect has not been studied well. Here, we investigated the porphyrin ligand effect on the Fe=O moiety with rR spectroscopy. The porphyrin ligand effect was modulated by the electron-withdrawing effect of the porphyrin substituent at the meso-position. This study shows that the frequency of the Fe=O stretching band, ν(Fe=O), hardly change even when the electron-withdrawing effect of the porphyrin substituent changes. This result is further supported by theoretical calculation of CompI. The natural atomic charge analysis reveals that the oxo and axial ligands work to buffer the electron-withdrawing effect of the porphyrin substituent. The electron-withdrawing porphyrin substituent shifts an electron population from the ferryl iron to the porphyrin, but the decreased electron population on the ferryl iron is compensated by the shift of the electron population from the oxo ligand and the axial ligand. The shift of the electron population makes the Fe-axial ligand bond length short, but the Fe=O bond length unchanged, resulting in the invariable ν(Fe=O) frequency.

Machine Learning-Based Interpretable Modeling for Subjective Emotional Dynamics Sensing Using Facial EMG.

Understanding the association between subjective emotional experiences and physiological signals is of practical and theoretical significance. Previous psychophysiological studies have shown a linear relationship between dynamic emotional valence experiences and facial electromyography (EMG) activities. However, whether and how subjective emotional valence dynamics relate to facial EMG changes nonlinearly remains unknown. To investigate this issue, we re-analyzed the data of two previous studies that measured dynamic valence ratings and facial EMG of the corrugator supercilii and zygomatic major muscles from 50 participants who viewed emotional film clips. We employed multilinear regression analyses and two nonlinear machine learning (ML) models: random forest and long short-term memory. In cross-validation, these ML models outperformed linear regression in terms of the mean squared error and correlation coefficient. Interpretation of the random forest model using the SHapley Additive exPlanation tool revealed nonlinear and interactive associations between several EMG features and subjective valence dynamics. These findings suggest that nonlinear ML models can better fit the relationship between subjective emotional valence dynamics and facial EMG than conventional linear models and highlight a nonlinear and complex relationship. The findings encourage emotion sensing using facial EMG and offer insight into the subjective-physiological association.

EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy.

Estrogen receptor-binding fragment associated antigen 9 (EBAG9) exerts tumor-promoting effects by inducing immune escape. We focused on the physiological functions of EBAG9 by investigating the bone phenotypes of Ebag9-knockout mice. Female Ebag9-knockout mice have fragile bones with lower bone mineral density (BMD) compared with wild-type mice. Histomorphometric analyses demonstrated that lower BMD was mainly caused by decreased bone formation. Serum bone turnover markers showed that enhanced bone resorption also contributed to this phenotype. We revealed that EBAG9 promoted autophagy in both osteoblastic and osteoclastic lineages. In addition, the knockdown of Tm9sf1, a gene encoding a protein that functionally interacts with EBAG9, suppressed autophagy and osteoblastic differentiation of the murine preosteoblastic cell line MC3T3-E1. Finally, overexpression of TM9SF1 rescued the suppression of autophagy caused by the silencing of Ebag9. These results suggest that EBAG9 plays a physiological role in bone maintenance by promoting autophagy together with its interactor TM9SF1.

Impairment of unconscious emotional processing after unilateral medial temporal structure resection.

The role of the amygdala in unconscious emotional processing remains a topic of debate. Past lesion studies have indicated that amygdala damage leads to impaired electrodermal activity in response to subliminally presented emotional stimuli. However, electrodermal activity can reflect both emotional and nonemotional processes. To provide behavioral evidence highlighting the critical role of the amygdala in unconscious emotional processing, we examined patients (n = 16) who had undergone unilateral resection of medial temporal lobe structures, including the amygdala. We utilized the subliminal affective priming paradigm in conjunction with unilateral visual presentation. Fearful or happy dynamic facial expressions were presented in unilateral visual fields for 30 ms, serving as negative or positive primes. Subsequently, neutral target faces were displayed, and participants were tasked with rating the valence of these targets. Positive primes, compared to negative ones, enhanced valence ratings of the target to a greater extent when they stimulated the intact hemisphere (i.e., were presented in the contralateral visual field of the intact hemisphere) than when they stimulated the resected hemisphere (i.e., were presented in the contralateral visual field of the resected hemisphere). These results suggest that the amygdala is causally involved in unconscious emotional processing.

An investigation of the modulatory effects of empathic and autistic traits on emotional and facial motor responses during live social interactions.

A close relationship between emotional contagion and spontaneous facial mimicry has been theoretically proposed and is supported by empirical data. Facial expressions are essential in terms of both emotional and motor synchrony. Previous studies have demonstrated that trait emotional empathy enhanced spontaneous facial mimicry, but the relationship between autistic traits and spontaneous mimicry remained controversial. Moreover, previous studies presented faces that were static or videotaped, which may lack the "liveliness" of real-life social interactions. We addressed this limitation by using an image relay system to present live performances and pre-recorded videos of smiling or frowning dynamic facial expressions to 94 healthy female participants. We assessed their subjective experiential valence and arousal ratings to infer the amplitude of emotional contagion. We measured the electromyographic activities of the zygomaticus major and corrugator supercilii muscles to estimate spontaneous facial mimicry. Individual differences measures included trait emotional empathy (empathic concern) and the autism-spectrum quotient. We did not find that live performances enhanced the modulatory effect of trait differences on emotional contagion or spontaneous facial mimicry. However, we found that a high trait empathic concern was associated with stronger emotional contagion and corrugator mimicry. We found no two-way interaction between the autism spectrum quotient and emotional condition, suggesting that autistic traits did not modulate emotional contagion or spontaneous facial mimicry. Our findings imply that previous findings regarding the relationship between emotional empathy and emotional contagion/spontaneous facial mimicry using videos and photos could be generalized to real-life interactions.

Peroral cholangioscopy for the evaluation of bile duct stricture in hepatocellular carcinoma on a preoperative examination.

Objective: Bile duct tumor thrombosis in hepatocellular carcinoma (HCC) is a relatively rare event with a poor prognosis. Furthermore, bile duct tumor thrombus in HCC may be misdiagnosed when only imaging modalities are used. The efficiency of peroral cholangioscopy (POCS) in evaluating bile duct lesions has been reported. Patients: We present three cases of HCC with bile duct strictures in which POCS was performed as a preoperative evaluation. Results: In these three cases, diagnosing whether the lesion was a bile duct tumor thrombus on CT and endoscopic retrograde cholangiopancreatography was difficult. We performed POCS in three cases and were able to diagnose the presence of bile duct tumor thrombus of HCC, including differentiation from extrinsic compression of the bile duct. Conclusion: POCS for HCC with bile duct features is useful for the preoperative diagnosis of bile duct tumor thrombus, especially in cases where the surgical procedure depends on the presence of bile duct tumor thrombus.

Altered emotional mind-body coherence in older adults.

Coherence between subjective experience and bodily responses in emotion is assumed to have a positive influence on well-being, which might be particularly valuable in late adulthood. Previous studies of young adults' continuous subjective, behavioral, and physiological responses to emotional films reported emotional mind-body coherence. In contrast, research regarding emotional coherence in older adults has been scarce. In this study, we examined emotional coherence in older adults between continuous valence ratings and behavioral responses (facial electromyography [EMG] of the corrugator supercilii and zygomatic major muscles), as well as between continuous arousal ratings and physiological measures (electrodermal activity [EDA] and fingertip temperature), in response to four emotion-eliciting film clips (anger, sadness, contentment, and amusement) film clips and an emotionally neutral clip. Intraindividual cross-correlation analyses revealed that the coherence between valence ratings and corrugator EMG activity for the anger-eliciting film was weaker in older adults than in young adults, who completed an identical experiment. Age differences also emerged in the coherence of arousal ratings with EDA and fingertip temperature measures, respectively, while participants watched the anger-eliciting and contentment-eliciting films; while negative correlations were found for older adults, positive correlations were found for young adults. These results indicate that emotional mind-body coherence somewhat differs quantitatively and qualitatively between older and young adults. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Brief research report: autistic traits modulate the rapid detection of punishment-associated neutral faces.

Speedy detection of faces with emotional value plays a fundamental role in social interactions. A few previous studies using a visual search paradigm have reported that individuals with high autistic traits (ATs), who are characterized by deficits in social interactions, demonstrated decreased detection performance for emotional facial expressions. However, whether ATs modulate the rapid detection of faces with emotional value remains inconclusive because emotional facial expressions involve salient visual features (i.e., a U-shaped mouth in a happy expression) that can facilitate visual attention. In order to disentangle the effects of visual factors from the rapid detection of emotional faces, we examined the rapid detection of neutral faces associated with emotional value among young adults with varying degrees of ATs in a visual search task. In the experiment, participants performed a learning task wherein neutral faces were paired with monetary reward, monetary punishment, or no monetary outcome, such that the neutral faces acquired positive, negative, or no emotional value, respectively. During the subsequent visual search task, previously learned neutral faces were presented as discrepant faces among newly presented neutral distractor faces, and the participants were asked to detect the discrepant faces. The results demonstrated a significant negative association between the degrees of ATs and an advantage in detecting punishment-associated neutral faces. This indicates the decreased detection of faces with negative value in individuals with higher ATs, which may contribute to their difficulty in making prompt responses in social situations.

Development of the RIKEN database for dynamic facial expressions with multiple angles.

The development of facial expressions with sensing information is progressing in multidisciplinary fields, such as psychology, affective computing, and cognitive science. Previous facial datasets have not simultaneously dealt with multiple theoretical views of emotion, individualized context, or multi-angle/depth information. We developed a new facial database (RIKEN facial expression database) that includes multiple theoretical views of emotions and expressers' individualized events with multi-angle and depth information. The RIKEN facial expression database contains recordings of 48 Japanese participants captured using ten Kinect cameras at 25 events. This study identified several valence-related facial patterns and found them consistent with previous research investigating the coherence between facial movements and internal states. This database represents an advancement in developing a new sensing system, conducting psychological experiments, and understanding the complexity of emotional events.

Asynchrony enhances uncanniness in human, android, and virtual dynamic facial expressions.

OBJECTIVE: Uncanniness plays a vital role in interactions with humans and artificial agents. Previous studies have shown that uncanniness is caused by a higher sensitivity to deviation or atypicality in specialized categories, such as faces or facial expressions, marked by configural processing. We hypothesized that asynchrony, understood as a temporal deviation in facial expression, could cause uncanniness in the facial expression. We also hypothesized that the effect of asynchrony could be disrupted through inversion. RESULTS: Sixty-four participants rated the uncanniness of synchronous or asynchronous dynamic face emotion expressions of human, android, or computer-generated (CG) actors, presented either upright or inverted. Asynchrony vs. synchrony expressions increased uncanniness for all upright expressions except for CG angry expressions. Inverted compared with upright presentations produced less evident asynchrony effects for human angry and android happy expressions. These results suggest that asynchrony can cause dynamic expressions to appear uncanny, which is related to configural processing but different across agents.

Cross-cultural differences in self-reported and behavioural emotional self-awareness between Japan and the UK.

OBJECTIVE: How we express and describe emotion is shaped by sociocultural norms. These sociocultural norms may also affect emotional self-awareness, i.e., how we identify and make sense of our own emotions. Previous studies have found lower emotional self-awareness in East Asian compared to Western samples using self-report measures. However, studies using behavioural methods did not provide clear evidence of reduced emotional self-awareness in East Asian groups. This may be due to different measurement tools capturing different facets of emotional self-awareness. RESULTS: To investigate this issue further, we compared the emotional self-awareness of Japanese (n = 29) and United Kingdom (UK) (n = 43) adults using the self-report Toronto Alexithymia Scale (TAS-20), alongside two behavioural measures - the Emotional Consistency Task (EC-Task) and the Photo Emotion Differentiation Task (PED-Task). Japanese adults showed higher TAS-20 scores than UK participants, indicating greater self-reported difficulties with emotional self-awareness. Japanese participants also had lower EC-Task scores than UK adults, indicating a lower ability to differentiate between levels of emotional intensity. PED-Task performance did not show clear group differences. These findings suggest that cross-cultural differences in emotional self-awareness vary with the task used, because different tasks assess distinct aspects of this ability. Future research should attempt to capture these different aspects of emotional self-awareness.

Sodium L-Aspartate Supplementation Improves Repeated-Sprint Performance.

Aspartate supplementation has been reported to improve endurance performance by facilitating the tricarboxylic acid cycle flux. The present study was performed to investigate the effects of aspartate supplementation on repeated-sprint performance and blood pH. Following an overnight fast, fourteen healthy males completed three sets of 10 × 6 s maximal sprints after consuming sodium L-aspartate (ASP) or placebo (PLA), in a double-blind manner. Both supplements were taken twice on each test day (2 × 4.5 g). Exercise performance (e.g., cadence and power output) and blood variables (e.g., pH and plasma amino acid levels) were measured. The ASP trial evidenced significantly higher plasma aspartate concentration during the first (ASP, 45.3 ± 9.2 µM; PLA, 6.1 ± 0.8 µM) and the second exercise sets (ASP, 24.2 ± 4.5 µM; PLA, 6.6 ± 0.9 µM) and peak cadence during the second set (ASP, 153 ± 3 rpm; PLA, 152 ± 3 rpm) compared with the PLA trial (all p < 0.05). The peak power output during the second exercise set (ASP, 743 ± 32 W; PLA, 734 ± 31 W; p = 0.060) and the blood pH immediately before (ASP, 7.280 ± 0.020; PLA, 7.248 ± 0.016; p = 0.087) and after the third exercise set (ASP, 7.274 ± 0.019; PLA, 7.242 ± 0.018; p = 0.093) tended to be higher in the ASP than in the PLA trial. In conclusion, ASP supplementation partially improved repeated-sprint performance (peak cadence during the second exercise set). However, it did not affect the mean power output.

Essential amino acid supplements ingestion has a positive effect on executive function after moderate-intensity aerobic exercise.

Aerobic exercise acutely improves cognitive function (e.g., executive function (EF); memory recognition (MR)) and increases circulating brain-derived neurotrophic factor (BDNF). In addition, branched-chain amino acids (BCAA) ingestion acutely shortens the choice reaction time and increases brain BDNF. We examined whether the ingestion of essential amino acid (EAA) supplements (mainly composed of BCAA) would positively impact on cognitive function and circulating BDNF after moderate-intensity aerobic exercise. Twenty-two healthy young men received either an EAA supplements or the placebo (PL) 30 min before undergoing aerobic exercise. The participants performed a cycling exercise at 60% of peak oxygen uptake for 30 min. EF after aerobic exercise was better after the EAA treatment than after the PL treatment (P = 0.02). MR (P = 0.38 for response accuracy; P = 0.15 for reaction time) and circulating BDNF (P = 0.59) were not altered by EAA supplements. EF improvement was correlated with increases in some amino acids (leucine, isoleucine, valine, lysine, phenylalanine; all Ps < 0.05) that are potential substrates for synthesizing neurotransmitters in the brain. These results suggest that EAA supplements ingestion had a positive effect on EF after moderate-intensity aerobic exercise, while MR and BDNF were not altered.

Influence of stimulus manipulation on conscious awareness of emotional facial expressions in the match-to-sample paradigm.

The conscious perception of emotional facial expressions plays an indispensable role in social interaction. However, previous psychological studies have reported inconsistent findings regarding whether conscious awareness is greater for emotional expressions than for neutral expressions. Furthermore, whether this phenomenon is attributable to emotional or visual factors remains unknown. To investigate these issues, we conducted five psychological experiments to test the conscious perception of emotional and neutral facial expressions using the match-to-sample paradigm. Facial stimuli were momentarily presented in the peripheral visual fields while participants read simultaneously presented letters in the central visual fields. The participants selected a perceived face from nine samples. The results of all experiments demonstrated that emotional expressions were more accurately identified than neutral expressions. Furthermore, Experiment 4 showed that angry expressions were identified more accurately than anti-angry expressions, which expressed neutral emotions with comparable physical changes to angry expressions. Experiment 5, testing the interaction between emotional expression and face direction, showed that angry expressions looking toward participants were more accurately identified than those looking away from participants, even though they were physically identical. These results suggest that the conscious awareness of emotional facial expressions is enhanced by their emotional significance.

A new model to estimate duration of survival in patients with hepatocellular carcinoma with BCLC intermediate stage.

It is difficult to determine whether an individual therapy contributes to the elongation of survival because of the difficulty of organizing clinical research in patients who receive multiple treatments in HCC. We aimed to establish a new model of survival prediction in patients with intermediate stage HCC to establish standards in the recent and coming multi-MTA era. This analysis was prepared using a data set of 753 patients diagnosed HCC prior to 2017. Multiple regression analysis showed age, naïve or recurrence, the size of the largest tumor nodule, the number of nodules, total bilirubin, albumin and α-fetoprotein as independent predictors of survival. A Weibull model had the best fit and, based on these predictors, we established a new predicted survival model. The survival duration can be predicted the proposed model; EXP (4.02580 + (- 0.0086253) × age + (- 0.34667) × (naïve/recurrence) + (- 0.034962) × (number of nodules) + (- 0.079447) × (the size of the largest nodule) + (- 0.21696) × (total bilirubin) + 0.27912 × (albumin) + (- 0.00014741) × (α-fetoprotein)) × (- natural logarithm(0.5))^0.67250. This model is useful for the planning and evaluating the efficacy of recent sequential therapies in multi-MTA era.

Sex differences in the rapid detection of neutral faces associated with emotional value.

BACKGROUND: Rapid detection of faces with emotional meaning is essential for understanding the emotions of others, possibly promoting successful interpersonal relationships. Although few studies have examined sex differences in the ability to detect emotional faces, it remains unclear whether faces with emotional meaning capture the attention of females and males differently, because emotional faces have visual saliency that modulates visual attention. To overcome this issue, we tested the rapid detection of the neutral faces associated with and without learned emotional value, which are all regarded as free from visual saliency. We examined sex differences in the rapid detection of the neutral female and male faces associated with emotional value. METHODS: First, young adult female and male participants completed an associative learning task in which neutral faces were associated with either monetary rewards, monetary punishments, or no monetary outcomes, such that the neutral faces acquired positive, negative, and no emotional value, respectively. Then, they engaged in a visual search task in which previously learned neutral faces were presented as discrepant faces among newly presented neutral distractor faces. During the visual search task, the participants were required to rapidly identify discrepant faces. RESULTS: Female and male participants exhibited comparable learning abilities. The visual search results demonstrated that female participants achieved rapid detection of neutral faces associated with emotional value irrespective of the sex of the faces presented, whereas male participants showed this ability only for male faces. CONCLUSIONS: Our results demonstrated that sex differences in the ability to rapidly detect neutral faces with emotional value were modulated by the sex of those faces. The results suggest greater sensitivity to faces with emotional significance in females, which might enrich interpersonal communication, regardless of sex.

Speedy detection of faces with emotional meaning plays a fundamental role in social interactions. However, it is unclear whether females and males differ in their ability to rapidly detect neutral faces associated with newly acquired emotional meaning/value. This study examined the sex differences in the rapid detection of neutral female and male faces associated with emotional value subsequent to associative learning. During learning, neutral faces were paired with monetary reward or punishment, such that they acquired positive or negative emotional value, respectively. In a subsequent visual search task, previously learned neutral faces were presented as discrepant faces among newly presented neutral faces, and the participants had to rapidly identify the discrepant faces. The results showed that, among female participants, neutral faces associated with reward and punishment were detected more rapidly than neutral faces not associated with monetary outcomes, irrespective of the sex of the face stimuli. By contrast, male participants only showed the rapid detection of neutral male faces. The results suggest enhanced sensitivity to faces with emotional meaning among females, which is consistent with the notion of greater sensitivity to emotional/social information in females.

Electromyographic Validation of Spontaneous Facial Mimicry Detection Using Automated Facial Action Coding.

Although electromyography (EMG) remains the standard, researchers have begun using automated facial action coding system (FACS) software to evaluate spontaneous facial mimicry despite the lack of evidence of its validity. Using the facial EMG of the zygomaticus major (ZM) as a standard, we confirmed the detection of spontaneous facial mimicry in action unit 12 (AU12, lip corner puller) via an automated FACS. Participants were alternately presented with real-time model performance and prerecorded videos of dynamic facial expressions, while simultaneous ZM signal and frontal facial videos were acquired. Facial videos were estimated for AU12 using FaceReader, Py-Feat, and OpenFace. The automated FACS is less sensitive and less accurate than facial EMG, but AU12 mimicking responses were significantly correlated with ZM responses. All three software programs detected enhanced facial mimicry by live performances. The AU12 time series showed a roughly 100 to 300 ms latency relative to the ZM. Our results suggested that while the automated FACS could not replace facial EMG in mimicry detection, it could serve a purpose for large effect sizes. Researchers should be cautious with the automated FACS outputs, especially when studying clinical populations. In addition, developers should consider the EMG validation of AU estimation as a benchmark.

[Social Allostasis Regulation by Robots].

Social allostasis, the process by which social interactions dynamically regulate psychophysiological states (e.g., emotions), is attracting attention. Social allostasis can significantly impact people's mental and physical wellbeing. However, adaptive social relationships are not necessarily available to all people. The use of robots is expected to solve this problem. In this paper, we present three studies of our group that prove the influence of social interactions with robots on human psychophysiological states. Our results showed that robots could amplify human subjective and physiological emotional responses through conversation, touch, and television co-viewing experiences. These results suggest that robots may be helpful for adaptive social allostasis regulation.

Differences in configural processing for human versus android dynamic facial expressions.

Humanlike androids can function as social agents in social situations and in experimental research. While some androids can imitate facial emotion expressions, it is unclear whether their expressions tap the same processing mechanisms utilized in human expression processing, for example configural processing. In this study, the effects of global inversion and asynchrony between facial features as configuration manipulations were compared in android and human dynamic emotion expressions. Seventy-five participants rated (1) angry and happy emotion recognition and (2) arousal and valence ratings of upright or inverted, synchronous or asynchronous, android or human agent dynamic emotion expressions. Asynchrony in dynamic expressions significantly decreased all ratings (except valence in angry expressions) in all human expressions, but did not affect android expressions. Inversion did not affect any measures regardless of agent type. These results suggest that dynamic facial expressions are processed in a synchrony-based configural manner for humans, but not for androids.

The inversion effect on the cubic humanness-uncanniness relation in humanlike agents.

The uncanny valley describes the typically nonlinear relation between the esthetic appeal of artificial entities and their human likeness. The effect has been attributed to specialized (configural) processing that increases sensitivity to deviations from human norms. We investigate this effect in computer-generated, humanlike android and human faces using dynamic facial expressions. Angry and happy expressions with varying degrees of synchrony were presented upright and inverted and rated on their eeriness, strangeness, and human likeness. A sigmoidal function of human likeness and uncanniness ("uncanny slope") was found for upright expressions and a linear relation for inverted faces. While the function is not indicative of an uncanny valley, the results support the view that configural processing moderates the effect of human likeness on uncanniness and extend its role to dynamic facial expressions.