ABSTRACT
Immunohistochemical and PCR analysis of the structure of lipid-containing hepatocytes and replication of chronic hepatitis C viral particles revealed no positive correlation between the presence of HCV RNA in blood specimens, number of infected hepatocytes (by expression of HCV NS3Ag) with clinical biochemical parameters and intensity of structural changes in biopsy specimens. Small-vesicular subcytolemmal lipid infiltration of hepatocytes was detected, presumably associated with hepatitis C virus replication phase. Characteristic subcytolemmal location of the lipid droplets can reflect their involvement in virus transport from the cell and indicate a virus-induced nature of lipid metabolism disorders.
Subject(s)
Hepatitis C, Chronic/pathology , Hepatocytes/virology , Lipid Metabolism , Liver/pathology , Adult , Aged , Female , Genotype , Hepacivirus/genetics , Hepacivirus/growth & development , Hepatitis C, Chronic/virology , Hepatocytes/pathology , Humans , Liver/cytology , Liver/virology , Male , Middle Aged , RNA, Viral/blood , Viral Nonstructural Proteins/biosynthesis , Virus Replication , Young AdultABSTRACT
Cell suspension consisting of cells from immature nervous and hemopoietic tissues was transplanted subarachnoidally to patients with craniocerebral injury aftereffects. In some patients cell therapy led to immune sensitization to donor antigens, detected by the leukocyte migration inhibition test. No signs of tissue-destructive autoimmune reactions were detected in patients receiving cell therapy. Follow-up of 56 patients showed that cell therapy was associated with significant improvement of the neurological status. No serious complications of this treatment modality were observed. Presumably, cell therapy is a safe method which can be used in the treatment of craniocerebral injury aftereffects.