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Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants.
Furukawa, Toru; Sawaguchi, Chiho; Watanabe, Aiko; Takahashi, Maki; Nigorikawa, Mutsumi; Furukawa, Kayoko; Iimura, Yosuke; Kajita, Shinya; Oguchi, Taichi; Ito, Yukihiro; Sonoki, Tomonori.
J Biosci Bioeng ; 116(5): 616-9, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23731869

ABSTRACT

We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency.


Subject(s)
Cellulose/metabolism , Laccase/metabolism , Lignin/metabolism , Oryza/metabolism , Laccase/genetics , Oryza/genetics , Plants, Genetically Modified , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Trametes/enzymology
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