[Evaluation of the effectiveness of children's health improvement in camps: actual problems and its solutions]. / Otsenka effektivnosti ozdorovleniya detei v lageryakh: sovremennye problemy i puti resheniya.

Currently, there is a decrease in the total number of organizations for summer recreation of children in the country while the share of organizations of group II of sanitary and epidemiological well-being among them remains at a consistently high level that determines the potential risk of harm to the health of the child population. At the same time, there is an increase in the number of children with a pronounced health-improving effect. So, it becomes necessary to analyze the information content of modern criteria for assessing the effectiveness of health improvement and to search for ways to resolve the noted contradictions. OBJECTIVE: Analysis and scientific and methodological substantiation of modern criteria for assessing the effectiveness of children's health improvement in recreation organizations (camps). MATERIAL AND METHODS: An analysis of the literature data, our own research results' systematization and effectiveness assessment of the health improvement of 195 children aged 10 to 15 years (112 girls and 83 boys) belonging to I and II health groups and vacationers in the inpatient organization of the Samara region in the 2019 summer campaign were carried out. Anthropometric, physio-metric, and bio-impedance metrics were studied in two stages (on the 1st or 2nd day of arrival and 1 day before the camp leaving). In a comparative analysis the differences were considered significant when p<0.05. RESULTS: Stationary rest and recreation organizations in the all-Russian structure of recreation organizations for children account for 5.8%. During staying in the camp, there was a significant decrease in the proportion of girls with underweight (p=0.039); among boys no significant changes were found. The general trend towards a change in the level of physical development is not statistically significant. Bio-impedance analysis revealed only 51.3% of children with changes in indicators characterizing the health-improving effect. The tendencies to change in the proportion of children with different levels of arm muscle strength and lung capacity during the staying in then camp are statistically significant. Correlations were established between muscle strength with basal metabolism (r=0.86) and muscle mass (r=0.86), as well as negative links between average strength and the percentage of adipose tissue (r= -0.52). During the staying in the camp, the 4 cases of acute respiratory infections were registered that did not affect the assessment of the recovery effectiveness. CONCLUSION: In order to assess the health-improving effect among children during one trial in the camp, the leading functional signs are muscle strength of the arms and vital capacity of the lungs. Additional criteria can be the morbidity during the staying in the camp, and in terms of the nutritional status analysis - the results of bio-impedance analysis. To assess the physical development at the beginning of the camp trial, the use of regional regression scales is informative that requires a corresponding explanation in the documents. In turn, the data from the baseline measurements should be used to build individual health measures during the staying in the camp.

[Hygienic characteristics of actual nutrition of the working population Samara Region].

The article presents the data of a retrospective analysis of the average per capita consumption of food by the population of the Samara region in comparison with the all-Russian indicators and recommended values of consumption in the period from 2010 to 2015, as well as the analysis of the actual nutrition of different groups of the working-age population of the Samara region in 2015 (2154 people aged 18 up to 62 years of age, 1179 of them women, 975 men) using the method of 24-hour reproduction of food and the frequency method with quantitative assessment of consumed products, in comparison with the dynamics of the prevalence of alimentary-dependent diseases. Estimation of parameters of nutritional status was also conducted on blood level of cholesterol and glucose, bioelectrical impedance analysis of body composition (n=514). It was found that 48.2% of the subjects had normal body weight, 38.1% -overweight, 13.7% - obesity. The analysis of actual consumption revealed an unfavorable trend of increasing consumption of fats, as well as sugar from 2010 to 2015, while the consumption of vegetables, fruits and berries was lowed compared to the national indicators and recommended consumption values. The analysis of actual nutrition allowed to make a conclusion about severe violations of the relationships between nutrients in the diet of all surveyed populations, characterized by the excess intake of fat (45% of the energy diet value), including saturated fatty acids, and added sugar (13% calories). In the diet of 82% of the respondents the content of vitamins B1 and B2 did not reach the recommended level, niacin - 37%, vitamin A - 47%, vitamin C - 68%, calcium and iron - 14%. With an increase in BMI, there was an increase in blood level of cholesterol and glucose (r=0.24 and r=0.26, respectively, p<0.05), both in men and women. In individuals with excess fat and added sugar in the diet, increased fat body component (r=0.17, p<0.05) and lowered content of skeletal muscle mass (r=0.25, p<0.05) were observed. The revealed unfavorable trend of nutritional and metabolic disorders, against the background of increased consumption of fats, added sugar, indicates the need for an integrated approach to the correction of diet at both individual and population levels.

[Evaluation of vitamin status of workers of oil-processing enterprises (Samara Region) according to the data on vitamin consumption and blood serum level].

Vitamin status of 77 employees of oil refineries (39 men and 38 women aged 22 to 62 years, body mass index - 25.0±4.7 kg/m2) has been assessed in autumn. The sufficiency with vitamins A, E, D, B2, B12, folate and beta-carotene was evaluated by determination in blood plasma, daily intake of vitamins B1, B2, niacin, C, A and betacarotene was calculated by frequency of food consumption during previous month. All surveyed were sufficiently supplied with vitamins A, B12 and folate (reduced level was detected in 0-2.6%). The content of tocopherols referred to triglycerides was reduced in 33% of the workers, in men it was 2 fold more likely (p<0.05) than in women (43.6 vs 21.1%). Vitamin D insufficiency took place in 23.4% of individuals, vitamin B2 and beta-carotene - in 44.2%. A reduced blood plasma content of one vitamin was detected in 39.0% of the surveyed, while two vitamins - in 24.7% persons, 2.2 fold more often (p<0.05) in men than in women. Multivitamin insufficiency was detected in 10.4% of workers. Reduced blood plasma level of at least one vitamin-antioxidant was detected in 46.8% (in 61.5% of men and 31.6% of women, p<0.05). 26.0% of surveyed were provided with all 6 vitamins and beta-carotene. Reduced relative to RDA daily intake of antioxidant vitamins (ascorbic acid and beta-carotene) was observed in 64.9 and 100% of individuals, B group vitamins (niacin, B2 and B1) - in 48.1-64.9%, vitamin A - in 42.9%.

[Assessment of the relationship of inflammation markers, adipokines and parameters of lipid metabolism in patient].

Objective - to assess the changes and the relationship of inflammation markers, adipokine levels and lipid metabolism in overweight and obesity, with signs of the metabolic syndrome. The study involved 62 women aged from 25 to 50 years. Group 1 included women (n=20) with a BMI<27.0, with no signs of metabolic syndrome, group 2 (n=20) - with a BMI>27.0, who were overweight or obese, with no signs of metabolic syndrome, 3 group (n=22) with a BMI>27.0, having overweight or obesity and the presence of any signs of metabolic syndrome. We determined relative and absolute lymphocyte counts and neutrophil granulocytes in the blood, lipid spectrum, blood serum levels of apolipoprotein A-1 (ApoA-1), and apolipoprotein B (apoB), leptin, adiponectin, interleukin-1ß (IL-1ß), interleukin 6 (IL-6), C-reactive protein (CRP). It was noted (p≤0.001) an increase of CRP in the blood of obese women, regardless of the presence of the metabolic syndrome: its concentration in groups 2 and 3 reached 6.69±3.03 and 7.30±3.08 mg/l, respectively, against 2.39±1.66 mg/l in group 1. Increasing BMI was accompanied by (p≤0.01) increase in erythrocyte sedimentation rate (8.1±5.0; 14.3±8.7; 13.9±8.5 mm per hour, respectively, in groups 1, 2 and 3). In women from groups 1 and 3 an increase in triglycerides, accompanied by a decrease in HDL (r=-0.44; p<0.025 and r=-0,57; p<0.001, respectively), and increase of LDL cholesterol (r=1.0; p<0.001) has been shown. The maximum number of positive correlations between indicators of lipid profile and inflammation markers was found in group 1, the minimum in group 3. This analysis showed the presence of chronic inflammation, which was accompanied by various disorders of lipid metabolism, even in the absence of clinical signs of pathology, the severity of which increased with increasing BMI and the development of metabolic syndrome.

[CLINICAL AND ENDOSCOPIC, MORPHOLOGIC AND IMMUNOHISTOCHEMICAL FEATURES OF GASTRIC ULCER IN H. PYLORI-INFECTED INDIVIDUALS RECEIVING CYTOTOXIC THERAPY].

THE PURPOSE OF THE STUDY: To determine the prognostic significance of the expression of molecules of PCNA, Bcl-2, NF-Kb and tachykinins (substance P, neurokinin A) in patients with gastric ulcer (CU) receiving cytotoxic therapy. MATERIALS AND METHODS: Total surveyed 90 patients divided into 3. equal groups. The first comparison group consisted of patients with chronic atrophic H. pylori-associated gastritis (CAG) (30 pers.). A second control group consisted of patients with gastric ulcer (30 pers.). Third, the study group consisted of 30 people. with CU suffering from hematological malignancies, in a period of complete clinical remission of the disease and receiving supportive polychemotherapy (PCT). Patients underwent endoscopy, morphological and immunohistochemical study of the mucous membrane of the antrum and body of the stomach to detect the expression of molecules of PCNA, Bcl-2, neurokinin A, substance P and factor Nf-Kb. RESULTS: The total level of dyspeptic syndrome on visual scale analogue in patients receiving chemotherapy and GU (GUpct) was significantly higher (p < 0.05) compared with patients with GU. It should be noted that patients with GUpct reducing clinical symptoms is much slower (p < 0.05). At the same time in 13 (43.3%) patients with GUpct determines the duration of ulcer healing, whereas in patients with GU in only 4 (13.3%) patients. Patients with GUpct more frequently (p < 0.05) were verified II and stage Ill chronic gastritis (CG), while Stage I--less (p < 0.05). Patients with GUpct significantly more often (p<0.05) was determined by the II degree of CG and significantly less (p < 0.05)--IV degree. Patients with GUpct determined significantly lower (p < 0.05), the expression performance PCNA, substance P and neurokinin A and higher (p < 0.05)--Bcl-2 and factor Nf-kB. CONCLUSION: GU in patients receiving chemotherapy, dyspeptic syndrome is characterized by severe, advanced stage of CG on the background of relatively low severity of CG in accordance with the classification of OLGA (2008). Patients with GUpht have a significant level of violation of regeneration changes how is this atrophy, intestinal metaplasia, dysplasia of gastric mucosa association with gross violations of the processes of epithelial cell homeostasis of epithelial cells regulation after molecules PCNA, Bcl-2, NF-kB and tachykinins (substation P, neurokinin A).

[Assessment of vitamin status of the workers of Samara thermal power plant according to data on vitamin intake and their levels in blood].

Sufficiency of 58 employees of a thermal power plant (47 men and 11 women aged 21 to 64 years, body mass index - 27.0±5.8 kg/m2), with vitamins A, E, D, B6, B12, folic acid and carotenoids (content in the blood plasma) and vitamin C (urinary excretion) was conducted in winter (February 2015). All surveyed workers were sufficiently supplied with vitamins A, B6, B12 and folic acid (a reduced level was detected in 0-9%). Insufficient supplying with vitamin E had 19% of the workers (and only men), vitamin C - 44%, vitamin D - 61%, carotenoids - 93%. Only 5% persons were sufficiently provided with all 6 vitamins. A combined deficiency of two vitamins had 38% of them, three - 22%, four - 16%. Simultaneously reduced plasma level of two antioxidants was observed in 36% of subjects, three - in 12%. Significant positive correlation (p <0.05) was detected between plasma levels: total cholesterol and retinol and ß-carotene; tocopherol and total cholesterol, triglycerides, low density lipoprotein cholesterol; between the concentration of vitamin D, and high density lipoproteins; levels of retinol and tocopherol and ß-carotene. In parallel intake of vitamins C, A, B1, B2 and niacin has been assessed by calculation of the frequency of food consumption during the previous month. Reduced relatively recommended daily intake of vitamins A, C has been found in approximately half of the surveyed, niacin, vitamin B1 and B2 - in 70-80%. Comparison of the data on the availability of vitamins C and A, obtained by calculation of vitamin intake and biochemical methods gave identical results in 55 and 60% of cases, correspondingly. The lack of vitamins has been detected more frequently by assessing dietary intake.

[The dependence of diet macronutrient composition and energy intake from human phase of the weekly cycle ­ weekdays/weekends].

When planning your diet regime is desirable to focus on the natural feeding behavior of the person. However, many aspects of eating behavior are not studied. The aim of this work was to study the changes of diet calorie and nutrient composition depending on the body mass index (BMI), time of day, week cycle phase: weekends/weekdays, and the availability of rational installation in nutrition. The study involved 67 women aged 29 to 49 years with a BMI of 18.3 to 34.2 kg/m2. Macronutrient composition and energy value of the diet were evaluated by analyzing the food diaries, which were filled by all respondents continuously for 8 workdays and 4 weekends. The results of this study showed no significant correlation between BMI and calorie intake, while a positive correlation was observed between BMI and fat component of the diet (r=0.362, p≤0.05). It was also found that the restriction of food intake in the evening (after 8 pm) did not lead to a decrease in total daily energy value of the diet, and was characterized by the consumption in the evening about 31% of the total fat intake. Diet energy value was significantly higher on weekends than during the week and was 2376±394 against 1940±402 kcal (p<0.05). According to the data obtained, compliance «restriction of supply in the evening¼ does not lead to a significant reduction in daily caloric intake.

[Habitat and incidence of respiratory organs diseases in the Samara population].

There was performed the assessment of habitat quality for Samara population. Risk factors for prevalence of respiratory diseases (pneumonia, allergic rhinitis, asthma) in children, teenagers and adults were revealed to be formaldehyde, phenol, hydrogen sulfide in the ambient, cadmium and copper in the soil.

[The impact of anthropogenic factors of environment on morbidity of diabetes mellitus].

The diabetes mellitus is a real threat to population health in all countries. In international practice studying and successful solutions of problems conditioned by this disease are related to development of registries. The Novosibirsk municipal registry of diabetes mellitus is an integral part of the Public registry. The data of municipal registry and analysis of conditions of environment were used to detect characteristics of morbidity of diabetes mellitus in population residing in the territories with different level of anthropogenic pollution by aero-pollutants. The technique of mapping was applied to develop thematic clinical hygienic map. The technique of the thematic clinical hygienic mapping broaden functional possibilities and sphere of application of territorial registry of diabetes mellitus. This technique opens new possibilities and perspectives in development of complex programs of prevention in the territories with anthropogenic pollution.

[To question about integrated prevention of diabetes taking into account the technogenic factors of environment (experimental results)].

Experimental conditions shown that formaldehyde decreased course of diabetes. Adding to meal the powder of plant's rhizome Curcuma longa contributes to the restoration of the morphological structure of the liver, kidney, the normalization of water-salt and carbohydrate metabolism, this can be used in programs for integrated prevention of diabetes in areas of technological risk.

[The estimation of risk of deficit consumption of proteins, vitamins and substance mineral adult population of Samara].

Survey of the brainwork workers with low physical activity has revealed the risks of inadequate consumption of fiber, calcium, iron, vitamins B1, B2, C, A, due to mismanagement of their consumption.

[Substantiation of the program of realization of bases of the state policy of a healthy nutrition of population of the Samara Region for the period till 2020 year].

Substantiate the need for long-term program of sanitation the diet of the population of the Samara Region. A systemic analysis of actual nutrition of different population groups in the region showed an excessive consumption of fats and added sugar--the main risk factors of obesity, cardiovascular diseases and diabetes mellitus. The risks of inadequate consumption of vitamins B, calcium, iron, caused by the irrational combination of the foodstuff use, are revealed.

[The alimentary status sisk of the hevophilia with the lesion of jointts].

The diagnostic findings sick of a hemophilia with recurring hemarthrosis are presented. At studying of componential structure of a body by a method of bioimpedansometriya considerable fluctuations of an index of weight of a body, a poor development of a muscular fabric, increase in percentage of a fatty fabric that is connected with low, physical activity and increase in the contribution of fat in power value of a food ration have been found out in patients. Results of research of an actual food by a frequency method with a quantitative estimation at sick of hemophilia and healthy students testify to probable risk of insufficient consumption of vitamins B1, B2 and calcium, magnesium that demands individual correction.

[A food and the food status of workers brainwork with low physical activity].

The data of the investigation of 340 average income men and women ages 18-60 whose labour is characterized by low power expenditure, are represented in this article. Their food ration contains about 13% of protein and more than 40% of fat, the most part of it contains the saturated fatty acids. 55% of men and 50% of women have got body mass index (BMI) more than 25; obesity is revealed with 7% of men and 18% of women. Glucose blood level is raised at 30% of the surveyed and is correlated with the consumption of the added sugar (r = 0.44; p < 0.05). The cholesterol maintenance in blood is above normal with 60% of the surveyed, cholesterol connection with the cholesterol food maintenance is not revealed.

[Role of alimentary factors in development of digestive system diseases].

In work the data are presented, which characterize the connection of a diet with disease of illnesses of digestive organs which rises in process of increase of products that contain gluten and meat reduction in diet. Immune reaction is found out, that shows the appearance of antibodies. A gladin and fabric transglutaminaze. Reaction characteristics are different in people with different blood groups.

[Unbalanced nutrition is a universal factor in the occurrence of visceral diseases].

Nutrition is one of the most important determinants of human health. The investigation has determined the pattern of actual nutrition of the Samara Region, its influence on the occurrence of cardiovascular, endocrine, urogenital, and musculoskeletal, and digestive diseases. Emphasis is placed on unbalanced diets excluding meat, by increasing the consumption of bread and cereals, which is a universal factor for the development of visceral diseases. Balanced nutrition using full-value natural products in the optimum ratio is a major condition for health preservation and a means for prevention of the development of various diseases.

[Fragments of functional proteins in the primary culture of human erythrocytes].

According to previously reported data, the supernatant of a primary culture of human erythrocytes contains 33 hemoglobin fragments. An analysis of the supernatant of a 20% (v/v) suspension of human erythrocytes allowed us to identify additionally four peptides whose precursors are cytoplasmic beta-actin (two fragments), fructose diphosphate aldolase B, and an unknown protein, as well as the amino acids tyrosine and tryptophan. The composition and the content of the components of the supernatant did not depend on the age or blood group of donors. The dynamics of accumulation in the supernatant (20-80 min of incubation) of the 14 hemoglobin fragments with the most reliably reproducible contents was obtained. The content of six peptides increased more than twofold between 20 and 40 min of incubation: the maximum increase in concentration was observed between 40 and 80 min (140%). The level of peptides that had the maximum concentration at the end of incubation was about 1000 pmol/ml of sedimented erythrocytes. The biological effects of the peptides identified in the supernatant of erythrocytes involve the stimulation of proliferation and hemopoiesis, suppression of proliferation, a bactericide effect, etc. These effects indicate the physiological importance of peptide release by erythrocytes. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2008, vol. 34, no. 2; see also http://www.maik.ru.

Endogenous fragment of hemoglobin, neokyotorphin, as cell growth factor.

It is shown that neokyotorphin (the alpha-globin fragment 137-141) stimulates proliferation of normal cells (murine embryonic fibroblasts, red bone marrow and spleen cells) and tumor cells (murine melanoma and transformed fibroblasts L929) in the absence or in the presence of fetal bovine serum. In contrast to serum deprivation conditions, the ability to potentiate L929 cell growth in the presence of fetal serum is strongly cell density dependent. The peptide also enhances the viability of L929 cells, murine embryonic fibroblasts and of the primary cultures of murine red bone marrow cells and splenocytes under serum-deprivation conditions for at least 72 h. The results of flow cytometry analysis suggest that the effect of neokyotorphin on survival of L929 cells in serum-free culture medium is due to maintenance of cell proliferation in the absence of growth factors. Along with cell cycle progression the peptide induces reversible reduction of L929 cell size.

[Optimization of the diagnosis and treatment of anemias under exposure to aromatic-type organic solvents]. / Optimizatsiia diagnostiki i lecheniia anemii v usloviiakh vozdeistviia organicheskikh rastvoritelei aromaticheskogo riada.

171 iron-deficient painters and 20 subjects with chronic intoxication with aromatic hydrocarbons were studied in a large aircraft construction factory. After correlative analysis of iron metabolism, ferritin, erythrocytes, diameter and perimeter of reticulocytes, length of service three types of iron-deficiency were identified: typical, with evident toxicity, with a high risk of toxic damage. An important role of lipid peroxidation activity and toxic erythrocyto-membranopathy for pathomorphosis of iron deficiency anemia and progress of toxic anemia is demonstrated. A differential approach to treatment of different anemia variants was used. The treatment included membranoprotectors (alpha-tocopherol, essential, lypostabil) and unspecific detoxication (SUMS-enterosorbent, unitiol).

[Fatty acids in erythrocyte membranes and the status of lipid peroxidation in painters]. / Sostav zhirnykh kislot v éritrotsitarnykh membranakh i sostoianie reaktsii peroksidnogo okisleniia lipidov u maliarov.

Spectrographic studies of fat acids in erythrocytic membranes and lipid peroxidation (LPO) contributed to the identification of different phases in the formation of damage effects. Exposition to toxic complexes (mostly solvents) in the first year caused a predominant growth of the antioxidant systems' activity characteristic of the processes of adaptation. In case with the work duration from one to five years, the u saturated fat acid content growth was accompanied by both pro- and antioxidative processes, which was indicative of the compensatory nature of the revealed changes. The most vivid shifts in the LPO reactions and the fat acids' content in erythrocytic membranes were found in the occupational group of patients engaged in the profession for more than 5 years. Those included major changes in the spectrum of both saturated and unsaturated acids, LPO activation with concomitant depression of the antioxidative systems.