ABSTRACT
The objective of the present study was to investigate ionic interactions between alginate and a monoclonal antibody (mAb1) and to utilize those interactions for the sustained release of mAb1. The existence of ionic interactions between alginate and mAb1 was strongly reflected by their rheological behavior. A 3-4 times increase in storage modulus (G') was observed by addition of 30 mg/mL mAb1 to a 20 mg/mL alginate solution. This increase was strongly dependent on pH and ionic strength. In vitro release studies revealed a marked pH-dependence of release rates and the reversibility of alginate-mAb1 complexation under physiological conditions. Two alginate-mAb1 sustained release formulations were developed by an internal gelation technique using CaCO(3) and CaHPO(4) as calcium sources for physical cross-linking. The CaCO(3) formulation provided a stable pH-environment, optimally suited for pH-sensitive proteins. CaHPO(4) led to a lower pH and stronger alginate-mAb1 interactions. The CaHPO(4) cross-linked alginate released mAb1 over a period of 10-15 days. The long release period and changes in viscoelastic properties of alginate, when being mixed with mAb1, indicate the incorporation of mAb1 molecules into a mixed network with alginate. The results of this study demonstrate that ionic interactions between polyanions and mAb1 are present and that they can be exploited for sustained release delivery of mAb1.
Subject(s)
Antibodies, Monoclonal/metabolism , Delayed-Action Preparations/metabolism , Polymers/metabolism , Recombinant Proteins/metabolism , Antibodies, Monoclonal/administration & dosage , Antibodies, Monoclonal/chemistry , Delayed-Action Preparations/administration & dosage , Delayed-Action Preparations/chemistry , Humans , Polyelectrolytes , Polymers/administration & dosage , Polymers/chemistry , Protein Binding/physiology , Recombinant Proteins/administration & dosage , Recombinant Proteins/chemistry , Rheology/methodsABSTRACT
From microscopic observations, it was established that an oil-in-water emulsion with droplets of a size in the micrometer range can spontaneously form at room temperature without additional external stirring as soon as a solvent that is only partly miscible to water-like dichloromethane (DCM) is put in contact with an aqueous mixture of polyethylene glycol (PEG) and a protein. Experimental results show that emulsification only occurs if the system simultaneously includes PEG with middle chain, an organic solvent partly miscible to water and for which PEG affinity is sufficiently high, and a protein. From adsorption kinetics, it appears that this spontaneous emulsification process is related to the rapid diffusion of DCM towards water through the formation of interfacial turbulences, once the accumulation of PEG close to the DCM/water interface occurs. The oil droplets formed would be then stabilized by adsorbed protein molecules. Since the presence of polylactic acid in the organic phase did not prevent the emulsion formation, we studied the feasibility of formulating microparticles using this polymer. From results, it appears that microcapsules with a polymeric shell, with a homogeneous size of about 50 microm and able to encapsulate a model hydrophobic drug, such as amiodarone, can be obtained by using this spontaneous emulsification method.