Effect of mango seeds as an untraditional source of energy on the productive performance of dairy Damascus goats.

Eighteen dairy Damascus goats weighing 38-45 kg live body weight and aged 3-4 years were divided into three groups according to their body weight, with six goats in each group. Yellow corn grain in their concentrate feed mixture was replaced with mango seeds (MS) at levels of 0% MS in group 1 (G1, control), 20% MS in group 2 (G2), and 40% MS in group 3 (G3). The digestibility coefficients of the organic matter, dry matter, crude fiber, crude protein, ether extract, nitrogen-free extract, and total digestible nutrients increased (P < 0.05) upon feeding MS to G2 and G3. The amounts of dry matter, total digestible nutrients, and digestible crude protein required per 1 kg 3.5% fat-corrected milk (FCM) were lower (P < 0.05) in G2 and G3 vs. G1. Actual milk and 3.5% FCM yield increased (P < 0.05) with the increasing MS dietary level. G2 and G3 had the highest significant (P < 0.05) total solids, total protein, non-protein nitrogen, casein, ash, fat, solids not fat, lactose, and calcium contents compared with G1. Replacing yellow corn grain with MS in G2 and G3 significantly (P < 0.05) decreased the cholesterol concentration and AST activity. Feeding MS increased the concentrations of caprioc, caprylic, capric, stearic, oleic, elaidic, and linoleic acids and decreased the concentrations of butyric, laueic, tridecanoic, myristic, myristoleic, pentadecanoic, heptadecanoic, cis-10-Heptadecanoic, cis-11-eicosenoic, linolenic, arachidonic, and lignoseric acids in the milk fat. The results show that the replacement of corn grain with MS improved the digestibility, milk yield, feed conversion, and economic efficiency, with no adverse effects on the performance of Damascus goats.

Serological Investigation on the Presence of Feline Coronavirus (FCoV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in Domestic Cats Living with COVID-19 Positive Owners in the UAE, 2022.

Feline coronavirus (FCoV) is widely circulating among domestic cats (Felis catus). The zoonotic origin of the emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and the biological characteristics of CoVs, including the ability to cross interspecies barriers, facilitate its emergence in different animals, including cats' populations. The current study is the first to report the serological investigation on the presence of FCoV and SARS-CoV-2 in domestic cats living with COVID-19-positive owners in the UAE. A total of 83 sera were collected from domestic cats living with COVID-19-positive owners (by RT-qPCR). The cats were sampled during the period between February and May 2022 in Al-Ain and Abu Dhabi Cities, UAE. Detection of FCoV and SARS-CoV-2 was carried out by enzyme-linked immunosorbent assay (ELISA). FCoV antibodies were detected in 54 samples (65%). The frequencies of FCoV were significantly higher in purebred cats (48%; 40/83) and in the cat group with outdoor access (49.4%; 41/83). SARS-CoV-2 seroprevalence in collected sera revealed 8 samples (9.6%) with positive results. Four samples (4.8%) showed positive results for both FCoV and SARS-CoV-2 antibodies. In conclusion, FCoV is widely circulating within cats' populations involved in the study. The antibodies for SARS-CoV-2 were detected in cats' populations but at a low prevalence rate. COVID-19-positive people should avoid close contact with their cats. Future serological testing of large cats' populations is crucial for providing a good understanding of COVID-19 dynamics in cats.

Bioactive Compounds from Marine Sponges: Fundamentals and Applications.

Marine sponges are sessile invertebrates that can be found in temperate, polar and tropical regions. They are known to be major contributors of bioactive compounds, which are discovered in and extracted from the marine environment. The compounds extracted from these sponges are known to exhibit various bioactivities, such as antimicrobial, antitumor and general cytotoxicity. For example, various compounds isolated from Theonella swinhoei have showcased various bioactivities, such as those that are antibacterial, antiviral and antifungal. In this review, we discuss bioactive compounds that have been identified from marine sponges that showcase the ability to act as antibacterial, antiviral, anti-malarial and antifungal agents against human pathogens and fish pathogens in the aquaculture industry. Moreover, the application of such compounds as antimicrobial agents in other veterinary commodities, such as poultry, cattle farming and domesticated cats, is discussed, along with a brief discussion regarding the mode of action of these compounds on the targeted sites in various pathogens. The bioactivity of the compounds discussed in this review is focused mainly on compounds that have been identified between 2000 and 2020 and includes the novel compounds discovered from 2018 to 2021.

Selenium distribution in camel blood and organs after different level of dietary selenium supplementation.

Eight young female camels shared in four groups of two 2 years received a basal diet enriched respectively with 0, 2, 4, and 8 mg selenium under sodium selenite form for 64 days. Feed intake was assessed daily; blood samples were taken on weekly basis. One camel from each group was killed at the end of the experiment. Se concentration in serum was increased significantly in the supplemented groups with an average of 176.3 +/- 18.0 ng/mL in the control group, 382.7 +/- 107.6 in the group receiving 2 mg Se, 519.8 +/- 168.4 in the group receiving 4 mg Se, and 533.4 +/- 158.6 in the group receiving 8 mg Se daily. For glutathione peroxidase (GSH-Px) activity, the control group (51.0 IU/g Hb) and the group receiving 2 mg (50.5 IU/g Hb) were significantly different than groups receiving 4 and 8 mg (respectively, 65.9 and 76.1 IU/g Hb). No significant variation occurred for vitamin E (mean, 0.56 +/- 0.23 ng/mL). Significant correlation between serum Se and GSH-Px was reported. Kidney was the richest organ in selenium followed by lung, spleen, and liver, but the increase in supplemented groups was more marked in liver and kidney. The hair seemed to be the best indicator of selenium intake in camel.

Effect of selenium supplementation on blood status and milk, urine, and fecal excretion in pregnant and lactating camel.

Ten pregnant female camels divided into two groups received, after a 2-week adaptation period, an oral selenium (Se) supplementation (0 and 2 mg, respectively) under sodium selenite form for 6 months from the three last months of gestation up to the three first months of lactation. Feed intake was assessed daily. Blood samples and body weight were taken on a biweekly basis, both in dams and their camel calves after parturition. Feces and urine samples were collected monthly and milk on a biweekly basis. The Se concentration in serum increased significantly in the supplemented group and was threefold higher than the concentration compared to the control group, respectively, 305.9 +/- 103.3 and 109.3 +/- 33.1 ng/mL. The selenium concentration increased in similar proportion in milk (86.4 +/- 39.1 ng/mL in the control group vs 167.1 +/- 97.3 ng/mL in treated group), in urine, and feces. The glutathione peroxidase (GSH-Px) activity varied between 18.1 +/- 8.7 IU/g hemoglobin (Hb) in control group and 47.5 +/- 25.6 IU/g Hb in treated group but decreased after parturition in both groups. Vitamin E did not change significantly and was, on average, 1.17 +/- 0.72 and 1.14 +/- 0.89 ng/mL in the control and treated groups, respectively. Significant correlations were reported between serum Se, milk Se, GSH-Px, and fecal and urinary excretion or concentration. Blood values in camel calves were similar to those of the dams. The results seemed to confirm the sensitivity of camel to Se supplementation with an important increase of selenium in serum and milk.

Selenium in camel--a review.

Requirements for trace minerals in camels, particularly selenium, are not well-known. Selenium supplementation using a pharmaceutical form or commercial mineral mixture is common practice in camels to address the cardiomyopathy often attributed to selenium deficiency. This supplementation is often empirical and based on estimated needs for cattle. Nowadays the use of selenium in animal foodstuffs is commonplace and further investigation of its metabolism (ingestion, dynamic of storage-destocking, excretion) in camels is warranted. The present review aimed to synthesize all the experimental research (comparative selenium status in cow and camel, response to different levels of supplementation at different physiological stages, excretion maternal transfer, experimental toxicosis) and field observations (deficiency, supplementation practices) undertaken in camels. The results underline the particularity of the unique metabolic profile of the camel and lead to practical recommendations for supplementation in camels, highlighting its relative sensitivity to excess Se intake at lower levels than in cattle. The maximal tolerable dose is 8 mg and the recommended doses range from 2 to 4 mg.

Effect of different selenium supplementation levels on selenium status in camel.

Twelve female camels divided into three groups received, after a 2-week adaptation period, an oral Se supplementation (0, 2, and 4 mg, respectively) under sodium selenite form for 3 months. Feed intake was assessed daily, blood samples and body weight were taken on a weekly basis, and feces and urine samples were collected every 2 weeks up to 1 month after the end of the supplementation period. The Se concentration in serum was increased significantly in supplemented groups. The maximum level was observed in the period of supplementation in the camel receiving 4 mg (492.5 ng/mL), which was fourfold higher than the value at the beginning of the trial (126 to 138.5 ng/mL according to the groups). The selenium concentration increased significantly in urine and feces but to a lesser extent. A similar trend was observed with glutathione-peroxidase (GSH-Px) values varying between 8.4 and 96.5 IU/g Hb. However, no difference occurred between the two groups receiving 2 or 4 mg Se at the supplementation period. Vitamin E (mean 1.13 +/- 0.61 microg/mL with range 0.27-3.09) did not change significantly. Significant correlations were reported between serum Se, GSH-Px, fecal, and urinary excretion or concentration.