ABSTRACT
Introdução: Evidências têm mostrado uma associação entre anemia e Diabetes Mellitus. Contudo, a relação entre anemia e Diabetes Mellitus Gestacional (DMG) ainda não está bem estabelecida, bem como sua repercussão na instabilidade genômica. Portanto, objetivou-se verificar a associação entre anemia e instabilidade genômica em mulheres com DMG atendidas em um hospital universitário. Métodos: Estudo transversal com mulheres apresentando diagnóstico de DMG que realizaram pré-natal no Hospital Universitário de Santa Maria (RS). Informações referentes ao DMG, anemia e suplementação de ferro foram obtidas nos prontuários. A instabilidade genômica foi avaliada pelo ensaio de citoma em micronúcleos em células bucais (BMCyt). Resultados: Das 44 gestantes avaliadas, 28,6% apresentaram anemia e 79,5% foram suplementadas com ferro. Das gestantes que realizaram suplementação, 75,0% não apresentaram anemia gestacional. Níveis de hemoglobina não se associaram com a instabilidade genomica (p > 0,05), mas foi observada uma associação entre brotos nucleares e os níveis de glicemia (r = 0,977; p = 0,003). Conclusão: Não foi verificado associação entre anemia e instabilidade genômica em mulheres com DMG.(AU)
Introduction: There is evidence of an association between anemia and diabetes mellitus. However, the relationship between anemia and gestational diabetes mellitus (GDM) remains to be established, as well as its impact on genomic instability. Therefore, we aimed to examine the association between anemia and genomic instability in women with GDM treated at a university hospital. Methods: A cross-sectional study of women with a diagnosis of GDM who received prenatal care at the University Hospital of Santa Maria, southern Brazil. Data on GDM, anemia, and iron supplementation were obtained from medical records. Genomic instability was assessed by the buccal micronucleus cytome (BMCyt) assay. Results: Of 44 pregnant women evaluated, 28.6% had anemia and 79.5% received iron supplementation; of the latter, 75.0% did not have gestational anemia. Hemoglobin levels were not associated with genomic instability (p > 0.05), but an association was found between nuclear buds and blood glucose levels (r = 0.977; p = 0.003). Conclusion: There was no association between anemia and genomic instability in women with GDM.(AU)
Subject(s)
Humans , Female , Pregnancy , Adult , Young Adult , Diabetes, Gestational/genetics , Genomic Instability , Anemia/genetics , Prenatal Care , Blood Glucose/analysis , DNA Damage , Hemoglobins/analysis , Cross-Sectional Studies , Anemia, Iron-Deficiency/complications , Anemia, Iron-Deficiency/diet therapy , Anemia, Iron-Deficiency/genetics , Iron, Dietary/therapeutic use , Anemia/complications , Anemia/diet therapyABSTRACT
The purpose of this study was to investigate the association between the Val16Ala superoxide dismutase manganese-dependent (SOD2) single nucleotide polymorphism (SNP) and sperm reproductive parameters in a sample of Brazilian men. A potential association between this polymorphism and some oxidative biochemical parameters as well as sperm plasma cell-free DNA (cfDNA) levels were also evaluated. The study was performed using semen samples obtained from male patients that had undergone semen analysis according to the 2010 World Health Organisation (WHO) recommendations and the Val16Ala-SOD2 SNP was genotyped by polymerase chain reaction (PCR). Oxidative parameters as well as cfDNA levels were spectrophotometrically and fluorimetrically determined. Statistical analysis included chi-square test, analysis of variance followed by Bonferroni post hoc test, as well as logistic regression multivariate analysis. Semen samples from 169 men (35.89 ± 7.33 years) were genotyped. The allelic frequencies were V= 0.485 (n = 97), A = 0.515 (n = 103), with statistically similar allelic frequencies to those of samples obtained from a general population: V = 0.509; A= 0.591. In general, AV samples presented lower numbers of sperm-altered parameters than homozygous sperm. Lipoperoxidation was higher in homozygous than heterozygous sperm samples. The results suggest that genetically caused S-HP imbalance could contribute to poor sperm quality and affect male fertility.