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1.
Peanut agglutinin affinity chromatography of Trypanosoma cruzi glycoproteins.
Marcipar, A J; Lentwojt, E; Segard, E; Afchain, D; Fruit, J; Capron, A.
Parasite Immunol ; 4(2): 109-15, 1982 Mar.
Article in English | MEDLINE | ID: mdl-6175943

ABSTRACT

Glycoproteins from Trypanosoma cruzi epimastigotes have been extracted by diiodosalycilic acid and lithium salts, and phenol-water biphasic partition. Peanut agglutinin has been used in a one step preparative method for fractionating the total extract in order to separate the so-called galactose-terminal glycoproteins. The different fractions have been studied by SDS electrophoresis, ultracentrifugation and immunoelectrophoresis techniques. The experimental immunogenicity, antigenicity and specificity of the PNA affinity fractions has been evaluated.


Subject(s)
Glycoproteins/isolation & purification , Trypanosoma cruzi/analysis , Animals , Antibody Formation , Chromatography, Affinity , Cross Reactions , Epitopes , Glycoproteins/immunology , Goats , Immunization , Lectins , Leishmania/immunology , Molecular Weight , Peanut Agglutinin , Rabbits , Trypanosoma/immunology
2.
An improved microcarrier for mass culture of human diploid cells.
David, A; Segard, E; Broun, G; Berneman, A; Horodniceanu, F.
Ann N Y Acad Sci ; 369: 61-4, 1981.
Article in English | MEDLINE | ID: mdl-6942785

Subject(s)
Culture Techniques/methods , Cell Division , Cell Line , Culture Techniques/instrumentation , Diploidy , Fibroblasts/physiology , Humans
3.
Preparation and properties of organophilic trypsin macro-inhibitors: diamidino-alpha, omega-diphenylcarbamyl-poly(ethylene glycol).
Takerkart, G; Segard, E; Monsigny, M.
FEBS Lett ; 42(2): 214-7, 1974 Jun 01.
Article in English | MEDLINE | ID: mdl-4853744

Subject(s)
Polyethylene Glycols/chemical synthesis , Trypsin Inhibitors/chemical synthesis , Amidines , Binding Sites , Binding, Competitive , Carbamates , Chromatography, Gel , Kinetics , Mathematics , Molecular Weight , Phenols , Polyethylene Glycols/pharmacology , Protein Binding , Spectrophotometry , Spectrophotometry, Infrared , Spectrophotometry, Ultraviolet , Time Factors , Trypsin/metabolism , Trypsin Inhibitors/pharmacology
4.
Partition of trypsin in two-phase systems containing a diamidino-alpha,omega-diphenylcarbamyl poly (ethylene glycol) as competitive inhibitor of trypsin.
Takerkart, G; Segard, E; Monsigny, M.
FEBS Lett ; 42(2): 218-20, 1974 Jun 01.
Article in English | MEDLINE | ID: mdl-4853547

Subject(s)
Polyethylene Glycols/pharmacology , Trypsin Inhibitors/pharmacology , Trypsin/metabolism , Amidines , Binding Sites , Binding, Competitive , Carbamates , Chymotrypsin/metabolism , Hydrogen-Ion Concentration , Kinetics , Molecular Weight , Osmolar Concentration , Phenols , Protein Binding , Solubility , Spectrophotometry, Ultraviolet
5.
[Preliminary research on variations in the level of cerebral RNA following reticular stimulation in the rat]. / Recherche préliminaire sur les variations de la teneur en ARN cérébral après stimulation réticulaire chez le rat.
Schmaltz, G; Segard, E; Montreuil, J; Bloch, V.
J Physiol (Paris) ; 61 Suppl 1: 174, 1969.
Article in French | MEDLINE | ID: mdl-5402038

Subject(s)
Brain/metabolism , RNA/metabolism , Reticular Formation/physiology , Animals , Electric Stimulation , Memory , RNA/analysis , Rats
6.
Laryngotracheobronchitis and sudden death in children.
Segard, E C; Koneman, E W.
Am J Clin Pathol ; 50(6): 695-9, 1968 Dec.
Article in English | MEDLINE | ID: mdl-5726126

Subject(s)
Bronchitis/pathology , Death, Sudden , Laryngitis/pathology , Larynx/pathology , Trachea/pathology , Tracheitis/pathology , Bronchitis/epidemiology , Child , Child, Preschool , Humans , Infant , Laryngitis/epidemiology , Lung/pathology , Male , Tracheitis/epidemiology
7.
[Studies on the spontaneously diffusible nucleic acids of the rat liver nucleus. Morphologic study. Demonstration of the nucleolar origin of the diffusible particles]. / Etudes sur les acides nucléiques spontanément diffusibles des noyaux du foie de Rat. Etude morphologique. Démonstration de l'origine nucléolaire des particules diffusibles.
Puvion, E; Defert-Kobus, W; Kerckaert, J P; Puvion-Dutilleul, F; Segard, E; Montreuil, J.
C R Acad Hebd Seances Acad Sci D ; 266(3): 278-81, 1968 Jan 15.
Article in French | MEDLINE | ID: mdl-4967256

Subject(s)
Cell Nucleus/analysis , DNA/analysis , Liver/analysis , RNA/analysis , Animals , Cell Nucleolus , Histocytochemistry , Microscopy, Electron , Microscopy, Phase-Contrast , Rats , Spectrophotometry , Ultracentrifugation
8.
[Studies on spontaneously diffusible nucleic acids of the nucleus isolated from rat hepatocytes. Characterization of 2 types of sedimentable particles and demonstration of conjugated forms of messenger RNA]. / Etudes sur les acides nucléiques spontanément diffusibles des noyaux isolés des hépatocytes de Rat. Caractérisation de deux types de particules sédimentables et mise en évidence de formes conjuguées d'ARN de type messager.
Defert-Kobus, W; Kerekaert, J P; Segard, E; Montreuil, J.
C R Acad Hebd Seances Acad Sci D ; 266(1): 31-3, 1968 Jan 03.
Article in French | MEDLINE | ID: mdl-4968174

Subject(s)
Cell Nucleus/analysis , Liver/analysis , Nucleic Acids/analysis , RNA, Messenger/analysis , Animals , Cytoplasm/analysis , Liver/cytology , Methods , Microsomes/metabolism , Nucleotides , RNA/analysis , Rats , Spectrophotometry , Ultracentrifugation
9.
[Selective labelling and microanalysis of terminal 3'-hydroxyls of polynucleotides]. / Marquage sélectif et microdosage de l'extrémité 3'-hydroxyle des polynucléotides.
Segard, E; Desreumaux, S.
Bull Soc Chim Biol (Paris) ; 50(11): 2183-5, 1968.
Article in French | MEDLINE | ID: mdl-5716419

Subject(s)
Nucleosides/analysis , Polynucleotides , Boron Compounds , Chromatography, Ion Exchange , Chromatography, Paper , Methods , Microchemistry , Oxidation-Reduction , Polynucleotides/analysis , Tritium
10.
[Study on the ribonucleic acids spontaneously diffusible from the nuclei isolated from rat hepatocytes. Characterization of the messenger ribonucleic acids and "nuclear ribosomes"]. / Etude sur les acides ribonucléiques spontanément diffusibles des noyaux isolés des hépatocytes de rat. Caractérisation des acides ribonucléiques messagers et de "ribosomes nucléaires".
Defert-Kobus, W; Segard, E; Montreuil, J.
C R Acad Hebd Seances Acad Sci D ; 263(11): 842-5, 1966 Sep 12.
Article in French | MEDLINE | ID: mdl-4958542

Subject(s)
Liver , RNA, Messenger , Ribosomes , Animals , Rats
11.
[Studies on the metabolism of isolated cells. Research on factors active on the incorporation of radiophosphoric acid in the RNA of rat hepatocytes]. / Etudes sur le métabolisme des cellules isolées. Recherche des facteurs agissant sur l'incorporation de l'acide radiophosphorique dans les ARN des hépatocytes de rat.
Mallevais, J P; Segard, E; Montreuil, J.
C R Acad Hebd Seances Acad Sci D ; 262(17): 1892-5, 1966 Apr 25.
Article in French | MEDLINE | ID: mdl-4957149

Subject(s)
Liver/cytology , Phosphates/metabolism , RNA/biosynthesis , Animals , In Vitro Techniques , Rats
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