Investigating the biodegradability of iodinated X-ray contrast media in simultaneous nitrification and denitrification system.

The present study investigated the biodegradation of three iodinated X-ray contrast media (ICM), namely, iopamidol, iohexol, and iopromide, in simultaneous nitrification-denitrification (SND) system maintained in a sequencing batch reactor (SBR). The results showed that variable aeration patterns (anoxic-aerobic-anoxic) and micro-aerobic condition were most effective in the biotransformation of ICM while achieving organic carbon and nitrogen removal. The highest removal efficiencies of iopamidol, iohexol, and iopromide were 48.24%, 47.75%, and 57.46%, respectively, in micro-aerobic condition. Iopamidol was highly resistant to biodegradation and possessed the lowest Kbio value, followed by iohexol and iopromide, regardless of operating conditions. The removal of iopamidol and iopromide was affected by the inhibition of nitrifiers. The transformation products after hydroxylation, dehydrogenation, and deiodination of ICM were detected in the treated effluent. Due to the addition of ICM, the abundance of denitrifier genera Rhodobacter and Unclassified Comamonadaceae increased, and the abundance of class TM7-3 decreased. The presence of ICM affected the microbial dynamics, and the diversity of microbes in SND resulted in improving the biodegradability of the compounds.

Fate and removal of iodinated X-ray contrast media in membrane bioreactor: Microbial dynamics and effects of different operational parameters.

Iodinated X-ray contrast media (ICM) are mainly used in medical sector, and their presence in environmental waters is a cause of concern as they are capable of forming highly toxic iodinated disinfection byproducts. In the present study, the removal mechanisms of the three ICM- iohexol, iopromide, and iopamidol were elucidated in a lab-scale aerobic membrane bioreactor (MBR). At steady-state operation (solids retention time (SRT)- 70 days, organic loading rate (OLR)- 0.80 KgCOD/m3-day, nitrogen loading rate (NLR)- 0.08 KgNH4-N/m3-day, hydraulic retention time (HRT)- 12 h), the average removal of iohexol and iopromide was found to be 34.9 and 45.2 %, respectively, whereas iopamidol proved to be highly recalcitrant in aerobic conditions of the MBR (removal <10 % in all phases of the MBR operation). Further, through batch kinetic studies and mass balance analysis, it was observed that ICM were primarily biotransformed in the MBR system and biosorption (Kd < 10 L/Kg) was negligible. The biodegradation rate coefficient values (Kbiol) of the ICM were found to be <0.65 L/g-d which indicate that biotransformation rate of ICM was slow. Increased OLR (1.60 KgCOD/m3-day) and reduced SRT (20 days) were found to negatively affect the removal of the ICM. Further, the removal of ICM was found to depend on its initial concentration, and the increment in the ammonium loading (0.16 KgNH4-N/m3-day) did not favor its removal. The dosing of ICM altered the microbial dynamics of the mixed liquor and reduced the microbial diversity and richness. Bdellovibrio, Zoogloea, and bacteria belonging to TM7-3 class, Cryomorphaceae and Hyphomonadaceae families may contribute in ICM biotransformation.

Biogas production and nutrients removal from slaughterhouse wastewater using integrated anaerobic and aerobic granular intermittent SBRs - Bioreactors stability and microbial dynamics.

Slaughterhouse wastewater (SWW) was effectively treated in sequential anaerobic and aerobic granular intermittent sequencing batch reactors (ASBR+ISBR) for 665 days at different HRTs (48 h, 32 h, 24 h, and 12 h). The ASBR was stable at each HRT but performed relatively well at 12 h (OLR - 7.8-9.8 kg COD/m3-d) in terms of pollutants removal and biogas production than previously conducted research. The average biogas production was about 17.3 L/day having 70-76 % of CH4 which could subsidize around 52 % of electricity demand while saving 103 US dollars/day if installed at full scale. In the case of post aerobic granular ISBR, carbon and nutrients removal (N&P) was achieved by enriching granules (1.7-2.2 mm) at low DO (0.5-0.8 mg/L) via the nitrite pathway. The ISBR was also well stable at 12 h HRT (average OLR of 2.1 kg COD/m3-d) and met the effluent discharge guidelines recommended by the Central Pollution Control Board of India. During steady-state conditions (12 h HRT), the average removal efficiencies for COD, TSS, O&G, TN, and PO4-P were 98.8 %, 96.4 %, 98.7 %, 93.4 %, and 86.6 % respectively from combined ASBR and ISBR. The microbial analysis confirmed Euryarchaeota, Proteobacteria, Firmicutes, Chloroflexi, Bacteroidetes, Planctomycetes, and Synergistetes as the dominant phyla in ASBR. Methanosaeta (21.56 %) and Methanosarcina (6.48 %) were the prevailing methanogens for CH4 production. The leading phyla observed in ISBR were Bacteroidetes, Proteobacteria, Firmicutes, Armatimonadetes, Verrucomicrobia, Chloroflexi, and Planctomycetes. Heterotrophic AOB (Thauera, Xanthomonadaceae, Pseudomonas, Sphingomonadaceae, and Rhodococcus) were mainly detected in the system for ammonia oxidation besides common autotrophic AOB. Similarly, a known PAO (Accumulibacter) was not identified but other PAO (Rhodocyclaceae, Dechloromonas, Pseudomonas, Flavobacteriaceae, and Sphingobacteriaceae) were prevalent inside aerobic granular ISBR that contributed to both carbon and nutrients removal. The results obtained would help implement the investigated reactor configurations at the pilot and full scale for SWW treatment.

Human health and ecological risk assessment of 98 pharmaceuticals and personal care products (PPCPs) detected in Indian surface and wastewaters.

The release of pharmaceuticals and personal care products (PPCPs) in environmental waters has become an urgent issue due to their pseudo-persistent traits. The present study was undertaken to conduct a screening-level risk assessment of 98 PPCPs, detected in different water matrices (treated wastewater, surface water, and groundwater) of India, for evaluating ecological risk (risk to fish, daphnia, and algae), human health risk, and antimicrobial resistance (AMR) selection risk by following risk quotient (RQ) based methodology. In the present study, 47% of the detected PPCPs in Indian waters were found to exert a possible risk (RQ > 1) to either aquatic species and human health, or cause AMR selection risk. 17 out of 25 antibiotics detected in the environmental waters were found to pose a threat of AMR selection. 11 out of 49 pharmaceuticals were found to exert human health risk from ingesting contaminated surface water, whereas only 2 pharmaceuticals out of 25 were found to exert risk from the intake of groundwater. Very high RQs (>1000) for few pharmaceuticals were obtained, signifying a great potential of the detected PPCPs in causing severe health concern, aquatic toxicity, and AMR spread. Within India, special attention needs to be given to the pharmaceutical hubs, as the environmental waters in these regions were found to be severely contaminated with drug residues resulting in extremely high RQs. The present study will be helpful in prioritizing the detected PPCPs in the environmental waters of India, for which immediate attention and enforceable guidelines are required.

Effects of pharmaceuticals on membrane bioreactor: Review on membrane fouling mechanisms and fouling control strategies.

Pharmaceuticals have become contaminants of emerging concern due to their toxicity towards aquatic life and pseudo persistent nature in the environment. Membrane bioreactor (MBR) is one such technology that has the potential to act as a barrier against the release of pharmaceuticals into the environment. Fouling is the deposition of the constituents of the mixed liquor on the membrane surface and it limit the world-wide applicability of MBRs. To remove foulant layer, aggressive chemicals and extra cost consideration in terms of energy are required. Extracellular polymeric substances (EPS) and soluble microbial products (SMP) are recognized as principal foulants. Presence of pharmaceuticals has been found to increase the fouling in MBRs. Fouling aggravates in proportion to the concentration of pharmaceuticals. Pharmaceuticals exert chemical stress in microbes, hence forcing them to secrete more EPS/SMP. Pharmaceuticals alter the composition of the foulants and affect microbial metabolism, thereby inflicting direct/indirect effects on fouling. Pharmaceuticals have been found to increase or decrease the size of sludge flocs, however the exact mechanism that govern the floc size change is yet to be understood. Different techniques such as coupling advanced oxidation processes with MBR, adding activated carbon, bioaugmenting MBR with quorum quenching strains have shown to reduce fouling in MBRs treating pharmaceutical wastewater. These fouling mitigation techniques work on reducing the EPS/SMP concentration, thereby alleviating fouling. The present review provides a comprehensive understanding of the effects induced by pharmaceuticals in the activated sludge characteristics and identifying the fouling mechanism. Furthermore, significant knowledge gaps and recent advances in fouling mitigation strategies are discussed. This review has also made an effort to highlight the positive aspect of the foulant layer in retaining pharmaceuticals and antibiotic resistance genes, thereby suggesting a possible delicate trade-off between the flux decline and enhanced removal of pharmaceuticals.

Comprehensive review on iodinated X-ray contrast media: Complete fate, occurrence, and formation of disinfection byproducts.

Iodinated contrast media (ICM) are drugs which are used in medical examinations for organ imaging purposes. Wastewater treatment plants (WWTPs) have shown incapability to remove ICM, and as a consequence, ICM and their transformation products (TPs) have been detected in environmental waters. ICM show limited biotransformation and low sorption potential. ICM can act as iodine source and can react with commonly used disinfectants such as chlorine in presence of organic matter to yield iodinated disinfection byproducts (IDBPs) which are more cytotoxic and genotoxic than conventionally known disinfection byproducts (DBPs). Even highly efficient advanced treatment systems have failed to completely mineralize ICM, and TPs that are more toxic than parent ICM are produced. This raises issues regarding the efficacy of existing treatment technologies and serious concern over disinfection of ICM containing waters. Realizing this, the current review aims to capture the attention of scientific community on areas of less focus. The review features in depth knowledge regarding complete environmental fate of ICM along with their existing treatment options.

Biological wastewater treatment (anaerobic-aerobic) technologies for safe discharge of treated slaughterhouse and meat processing wastewater.

Slaughterhouse industry generates considerable amount of wastewater rich in proteins, lipids, fibres, and carbohydrates. Numerous technologies such as electrocoagulation, membrane separation, advanced oxidation, physico-chemical processes, and biological treatment have been implemented for reducing the concentrations of these compounds. Nevertheless, this review aims to provide extensive information solely on the biological treatment (anaerobic and aerobic) of slaughterhouse wastewater. The advantages of anaerobic treatment are excellent organic matter removal, less sludge production, low energy requirement, execution of higher loading rates, and considerable production of biogas. Aerobic treatment on the other hand is a less sensitive process, possess lower start-up period, and efficient nutrient removal process. Numerous case studies are described to bestow maximum understanding of the wastewater characteristics, kind of treatment employed, and complications involved in managing and treating of slaughterhouse effluent. Additionally, role of microbial community involved in the treatment of slaughterhouse waste is also discussed. Sequential anaerobic and aerobic reactors are also reviewed in order to present their advantages over single bioreactors. Intermittent sequencing batch reactor is a promising technology than other high rate digesters in the removal of carbon, nitrogen, and phosphorous.

Development of denitrifying phosphate accumulating and anammox micro-organisms in anaerobic hybrid reactor for removal of nutrients from low strength domestic sewage.

Low strength domestic sewage was treated in an Anaerobic Hybrid Reactor. The first phase was focused on the enhancement of denitrifying phosphate accumulating organisms (DPAOs) for the concurrent removal of nitrogen and phosphate. 16S rRNA gene confirmed the presence of Flavobacterium spp. and Pseudomonasalcaligenes spp. which are dominant DPAOs. The second phase was the anaerobic ammonium oxidation (anammox) enrichment phase, and it exhibited much higher chemical oxygen demand (87%) and nitrogen removal (90%) as compared to the first phase. However, it had failed to remove the phosphate from the system. In case of anammox, the dominant specie detected was Candidatus Brocadia, along with minor counts of Candidatus Jettenia and Anammoxoglobus Propionicus. Apart from that, ammonia oxidizing bacteria (Nitrosomonas europaea, Nitrosomonas nitrosa) and methanogens (Methanosaeta, Methanobacterium) were also detected in the system. This study showed the feasibility of anammox species over DPAOs in treating domestic sewage.