[On the similarity in the effects of different cryopreservation conditions on the growth and development of plants].

The morphological characteristics of 35 wild plant species were studied after freezing of seeds under the conditions of deep, fast, and programmed freezing (-196 degrees C) and non-deep freezing (-10 degrees C). The seeds were stored frozen for a month. The seeds of all the species were characterized by a low humidity. The field and laboratory seed germination capacity, leaf growth, the quantity and length of shoots, the quantity of generative organs, and the variability of these characteristics were studied. It was shown that the direction of changes under different cooling conditions was the same except for the laboratory germination capacity of some species. The direction was determined by the species features rather than cooling conditions.

[Effect of freezing of seeds on the morphological characteristics of four pink species].

The effect of non-deep freezing (-10 degrees C) and deep freezing (-196 degrees C) on the morphological characteristics of four pink species was investigated. It was shown that the growth and development of plants after cryopreservation changed insignificantly. Rather stable characteristics (diameter of flower, the number of cauline nodes, and root length) did not change. The length of generative shoots decreased a little, and the character of histograms changed. On the other hand, the changeable characteristics (the number of vegetative shoots and flowers) decreased after seed cryopreservation. However, the number of flowers did not vary in the next year. The parameters essential for seed cryopreservation, seed germination and the quantity of fruits, did not changed. It was also found that seed freezing exerts a stimulating effect on seed germination and the top limit of some parameters, including the quantity of generative organs.

[The investigation of the influence of cryopreservation and inbreeding on the variability of morphological characteristics of the evening-primrose biennial (Oenothera biennis L.)].

The effect of deep freezing of seeds at -196 degrees C (-320.8 degrees Fahrenheit) and inbreeding on the morphological characteristics of the evening-primrose biennal (Oenothera biennis L.), such as the size of plant parts and the amount of fruits, cauline nodes, and generative and vegetative shoots was investigated. The variation coefficients for these characteristics after treatment with low temperatures and inbreeding were calculated. It was shown that the characteristics of plant size show a low and a middle level of variability in the control group. The variation curves for these characteristics are similar to normal distribution curves. After stresses they slightly change or remain invariant. Large adventive shoots show a high level of variability. The distribution of the results in this case significantly differs from the normal. The branching of plants changes after both stress factors: the amount of all kinds of shoots decreases by half or even more.