ABSTRACT
A series of 1-[(4-benzyloxyphenyl)-but-3-enyl]-1H-azoles has been identified as potent antitubercular agents against Mycobacterium tuberculosis. Synthesis of compounds involved acid catalyzed ring-opening of cyclopropyl ring of phenyl cyclopropyl methanols followed by nucleophilic attack of the azoles on the carbocation intermediates. Several of the compounds 26, 34, and 36 exhibited significant antitubercular activities with MIC value as low as 1.56, 1.56, and 0.61 µg/mL, respectively, comparable to many standard drugs. These compounds were also screened against other strains of bacteria and fungi, and few of them showed good antifungal activity against A. fumigatus, responsible for lung infection.
ABSTRACT
Dactylogyrus species (Dactylogyrideae: Monogenea) are a group of monogenean gill parasites these parasites are highly host specific to freshwater fish of family Cyprinidae. A method is presented for the isolation and morphometric analysis of hamuli marginal hooks and bars form individual dactylogrids monogenoidea using phase contrast microcopy and camera lucida drawing, while simultaneously processing for rDNA analysis using the polymerase chain reaction. The identification and taxonomy of the worms in question was done by the morphometrical methods and rDNA analysis. ITS-1 regions of the rDNA gene of worms were amplified, sequenced and then the discrimination was recorded at the species level. The studied segments were compared with the published data on such segments of other Dactylogyrids species and were found to have significant relationship in the genes and spacer regions of the rDNA.
