ABSTRACT
The effects of transformation of downy birch (Betula pubescens Ehrh.) with the GS1 gene encoding the cytosolic form of glutamine synthetase on the rooting of plants in vitro was studied. The transgenic plants had an elevated content of glutamine as well as glutamic and aspartic acids and rooted more rapidly than the control plants. Rooting on a medium containing the glutamine synthetase inhibitor phosphinothricin prevented the accumulation of auxin in birch plants carrying the GS1 gene, indicating the involvement of this enzyme in raising the level of auxins in the transgenic plants. The correlation between the increase in the auxin levels in the transgenic plants carrying the glutamine synthetase gene and the increase in the rooting rate is shown for the first time.
Subject(s)
Betula , Glutamate-Ammonia Ligase , Indoleacetic Acids/metabolism , Plant Proteins , Plant Roots , Plants, Genetically Modified , Betula/enzymology , Betula/genetics , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Plant , Glutamate-Ammonia Ligase/biosynthesis , Glutamate-Ammonia Ligase/genetics , Plant Proteins/biosynthesis , Plant Proteins/genetics , Plant Roots/enzymology , Plant Roots/genetics , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/geneticsABSTRACT
Polyphenolic compounds present in extracts of plants belonging to the families Geraniaceae (blood-red cranesbill, wood cranesbill, meadow cranesbill, and alfilaria) and Rosaceae (red raspberry, European dewberry, and tormentil) have been tested for their activity against gram-positive and gram-negative bacteria of the genera Azotobacter, Bacillus, and Pseudomonas. The bacteriostatic activity exhibited some species-related features and depended on the polarity of the extracting agent. The bacteriostatic activity of plant-derived phenolic compounds correlated with their antioxidant potential. The plants of the families Geraniaceae and Rosaceae offer promise as a source of raw material for isolation of polyphenolic compounds exhibiting bactericidal activity, including against opportunistic pathogens (B. cereus, E. coli, P. aeruginosa, and S. aureus strains).