CYTOLOGIC CHARACTERISTIC OF THE CELLULAR COMPOSITION OF THE GUM MUCOUS MEMBRANE IN SCHOOL-AGE CHILDREN.

OBJECTIVE: The aim: To establish the characteristics of the gingival mucosa cellular composition in school-age children. PATIENTS AND METHODS: Materials and methods: We made a dental and cytological examination on 150 Ukrainian (Poltava city) children aged 6, 12, and 15 years. Smears were got by scraping from the gum mucosa and stained with a May-Grunwald solution. Cytograms were made by using a Biorex-3 BM-500T microscope with a DCM-900 digital microphotographic attachment. The programs were adapted for these types of studies. We used a magnification of 1000. The t-test for paired samples was used to compare values. The difference was considered statistically significant at P<0.05. RESULTS: Results: The number of superficial epitheliocytes in children aged 12 and 15 was significantly higher than in 6-year-olds but did not differ from each other. CONCLUSION: Conclusions: The process of physiological keratinization of the cells of the gingival mucosa in schoolchildren is diverse and keratinization occurs both due to the phenomenon of physiological necrosis, namely, apoptosis of surface cells and due to orthokeratosis. From the age of 6, the average number of surface cells in cytograms decreases, while the number of intermediate and dead keratinized cells increases up to 12 years and remains on the previous level in children of 15 years.

FUNCTIONING OF NO-CYCLE IN THE ORAL FLUID IN CHILDREN OF PRIMARY SCHOOL AGE WITH TYPE 1 DIABETES MELLITUS IN THE TREATMENT OF CHRONIC CATARRHAL GINGIVITIS.

OBJECTIVE: The aim: To determine the activity of NO-synthase and arginase in oral fluid in children with type 1 diabetes mellitus and to evaluate the efficacy of the treatment scheme we elaborated in the treatment of chronic catarrhal gingivitis. PATIENTS AND METHODS: Materials and methods: 82 children were examined, they were divided into groups by presence of gingivitis and diabetes mellitus. NO-synthase (NOS) activity was determined in oral fluid by the difference in nitrite concentration before and after incubation. The arginase activity was determined in oral fluid by the difference in the concentration of L-ornithine before and after incubation. RESULTS: Results: Use our treatment scheme in children with chronic catarrhal gingivitis and type 1 diabetes mellitus lead to a change in the polarization of oral macrophages towards the predominance of M2 polarization in 1 month. The polarization of macrophages changed to the predominance of M1 polarization activity in 1 year. CONCLUSION: Conclusions: We have elaborated a scheme for the treatment of chronic catarrhal gingivitis in children with type 1 diabetes mellitus. It normalizes the polarization of oral macrophages caused by exposure to chronic catarrhal gingivitis as a local pathogenetic factor.

THE LEVEL OF INTERLEUKIN-18 IN THE ORAL FLUID IN PRIMARY SCHOOL CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS AND TYPE I DIABETES MELLITUS.

OBJECTIVE: The aim: Of our research work was to study the level of proinflammatory interleukin-18 (IL-18) in the oral fluid of children with type I diabetes mellitus (DM), and to determine their periodontal status and the level of oral hygiene. PATIENTS AND METHODS: Materials and methods: 82 children were examined, they were divided into groups by presence of gingivitis and diabetes mellitus. The level of interleukin-18 in oral fluid was determined by immunoassay. RESULTS: Results: In patients with chronic catarrhal gingivitis and type I diabetes mellitus the level of interleukin-18 in oral fluid is the highest (70.91±7.48 pg / ml); the level of interleukin-18 in children with diabetes mellitus and healthy gums is high enough too, it is 14.87±1.11 pg / ml. Interleukin-18 is 3.41±0.25 pg / ml in healthy children with healthy gums. It is 5.74±0.27 pg / ml in somatically healthy children with chronic catarrhal gingivitis. CONCLUSION: Conclusions: We indicated that an increase in the value of interleukin-18 in oral fluid is associated with the presence of diabetes mellitus in children. Moreover, this cytokine can be considered as a potential biomarker of gum inflammation in children with diabetes mellitus.

CLINICAL AND CYTOLOGICAL CHARACTERISTICS OF THE GUMS IN CHILDREN OF PRIMARY SCHOOL AGE WITH NORMAL BODY WEIGHT AND OVERWEIGHT.

OBJECTIVE: The aim of the research was to study the cellular composition of the gums in children of primary school age with normal body weight and overweight for further use of this data in the early diagnostics of periodontal diseases. PATIENTS AND METHODS: Materials and methods: We examined 81 children aged from 6 to 12 years. Cytological examination of gingival cytograms was performed in all examined children. RESULTS: Results: Based on the analysis of the quantitative content of epithelial cells in children with normal body weight, their ratio was established, which is determined by the percentage of 0: 6: 94 (parabasal, intermediate, superficial). The obtained data completely coincide with the percentage of the differentiated ratio of epitheliocytes of multilayered squamous epithelium in children with normal body weight with inflammation and without it in the periodontal tissues. Our cytological examinations of gingival scrape smears in overweight children in contrast to the results of the study of epithelial scrape smears in children with normal body weight have some differences. Thus, in the process of calculation, the degree of differentiation of various epitheliocytes determines their percentage as follows - 3: 7: 90 (parabasal, intermediate, superficial) for children with gingivitis, and 2: 5: 93 (parabasal, intermediate, superficial) for children without inflammation in the periodontal tissues. CONCLUSION: Conclusions: The obtained results allowed us to conclude that in overweight children, in contrast to children with normal body weight, the number of parabasal cells decreases, and the number of superficial and intermediate cells increases.

THE BALANCE OF IL-1ß, IL-10 AND THE LEVEL OF IKBα EXPRESSION IN CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS AND GASTRODUODENITIS.

OBJECTIVE: The aim: To define the level of pro- and anti-inflammatory IL-1ß, IL-10 in the oral fluid of children with chronic gastroduodenitis, depending on the level of IkBα expression. PATIENTS AND METHODS: Materials and methods: Studiing the oral fluid and scraping of the gums of 50 children 6-12 years old was carried out to determine the level of IL-1ß, IL-10 and IkBα. The children were divided into 3 groups. Group 1 - 10 children with healthy periodontium and without somatic diseases. 2nd group - 20 somatically healthy children with chronic catarrhal gingivitis. The third group (20 children) of children with gastroduodenitis and chronic catarrhal gingivitis. The determination of the mRNA expression of the IkBα geneand the level of IL-10 and IL-1ß in the oral fluid was carried out by real-time PCR. RESULTS: Results: We determined that the level of pro-inflammatory IL-1ß in the oral fluid of primary school children had different levels in accordance with the state of dental and somatic health. It was the lowest in somatically healthy children without signs of gingivitis. We revealed that the level of IL-10 has anti-inflammatory properties, and an inverse relationship with the concentration of IL-1ß in the oral fluid of examined children. Analysis of IkBα expression made it possible to reveal its suppression in children of the second and third groups compared with healthy children. CONCLUSION: Conclusions: We found changes in the levels of IkBα in the gums of children with chronic catarrhal gingivitis; attenuated IkBα expression may lead to the deregulation of NF-κB pathways in the pathogenesis of periodontitis. Decreased IkBα expression may affect cytokine production and inflammatory response associated with chronic catarrhal gingivitis and with chronic gastroduodenitis.

THE VALUE OF DENTAL EXAMINATION FOR EARLY DIAGNOSIS OF THE MUSCULOSKELETAL SYSTEM DISEASES.

OBJECTIVE: The aim: The purpose of the study was to determine the correspondence between the location of the tongue median line and the degree of distortion of the vertebral column at different levels in children with scoliosis. PATIENTS AND METHODS: Materials and methods: Totally 56 children (30 girls, 26 boys) aged 11-14 years were examined, with a duration of musculoskeletal disorders 1-3 years. For each participant an oral cavity examination was performed, the position of the middle line of the tongue was determined. X-ray images of vertebral column of the patients from investigated groups were analyzed. RESULTS: Results: In the group with 1year duration of the disease 25.02% patients had a violation of the medial line of the tongue, in the group with 2 years of duration of the disease - 65,52% participants had the deviation of the medial line of the tongue, in the group with 3 years of duration of the disease 86.66% of patients had the deviation of the medial line of the tongue. CONCLUSION: Conclusions: This research allows us to recommend paying attention not only to the size, color, state of the papillae of the tongue, but also to the position of the middle line, which distortion may significate the deviation of the vertebral column in different portions.

The study of oral fluid dynamic parameters on the background of pathological and physiological dental abrasion.

OBJECTIVE: Introduction: Violation of oral fluid mineralization processes, which is determined by the mineralization potential of saliva, is associated with changes in the physicochemical parameters of the oral fluid, particularly its viscosity. The aim of our study was to study mineralization potential and types of microstallation of oral fluid as one of the factors of influence on the cariesogenic situation in the oral cavity of patients with physiological or pathological tooth abrasion. PATIENTS AND METHODS: Materials and methods: During the examination of patients' oral cavity, a comprehensive assessment of tooth hard tissues was performed in order to of study activity and prevalence of the processes occurring in them. RESULTS: Results: Assessing the physico-chemical parameters of oral fluid in patients of the first experimental group, we obtained the following results: the viscosity of saliva in subgroups was 2,17 ± 0,87 for subgroup #1, 1,78 ± 0,57 for subgroup #2, and 2,15 ± 0,86 for #3 subgroups, which did not have a significant difference between the indices within the group. During the research, the number of independent structures of oral fluid in subgroups 1-3 was 1.67 ± 0.86, 1.67 ± 0.77 and 1.57 ± 0.85. CONCLUSION: Conclusions: Thus, we have established that the mineralizing function of saliva changes when the cariesogenic situation in the oral cavity arises,. This leads to destabilization of the crystalline structure of the oral fluid and indicates the relationship between its structural and mineralizing properties.

Consideration of childhood psychological factors at dental appointment.

OBJECTIVE: Introduction: Paediatric dentistry is a special field of dental practice. The scope of professional activities within this field covers not only high level of expertise and technical skills tailored to the needs of young patients, but requires searching and creating positive psychological environment and communicative management of each child in order to improve daily service methods. The aim of this study is to identify and describe the main psychological characteristics of the age developmental periods of child's personality that dental professionals should regard dealing with the paediatric patients. PATIENTS AND METHODS: Materials and methods: The study involved 124 paediatric participants aged 2,5 - 15 years, who were referred to get specialized dental care at the Department of Paediatric Dentistry at the Municipal Paediatric Dentistry Hospital, Poltava. We have collected and identified behavioural patterns of children of all age groups during their contacts with dentists at the dental offices as well as studied the characteristics of childhood psyche age periods through the available literature. The data obtained were thoroughly As a result of the analysis of the obtained data, the principles for managing paediatric patients' behaviour at the dental office have been elaborated and introduced into the practice. RESULTS: Results: Outer evaluation of the children's activities performed is perceived by children as an assessment of personality, so any negative doctor's remark can provoke anxiety, irritability, and discomfort, apathy. Therefore, the paediatric specialists should remain attentive to physical and emotional indicators of stress when dealing with young patients. CONCLUSION: Conclusions: Hence, among the key tasks of a paediatric dentist is to create the friendliest and most comfortable conditions for small patients, where they have the opportunity to play toys and receive little gifts for patience and courage.

[Morphological study of enamel and dentin teeth with carious process and non-carious lesions].

OBJECTIVE: Introduction: The condition of hard teeth tissues while choosing treatment method becomes particularly important due to the large development of adhesive technologies and the choice of filling materials. The quality of adhesion is one of the key factors that affects effectiveness of the entire range of therapeutic interventions, that determines the success of therapeutic treatment and the reliability of therapeutic restorations fixation. The aim of the work is to study the condition of hard teeth tissues in carious and non-carious pathology for further rationale of the choice of filling material and adhesive system. PATIENTS AND METHODS: Materials and methods: A thorough examination included teeth that were removed according to the indications. The age of patients, whose teeth were examined, was 25-55 years old according to the classification of age periods adopted at the International Symposium on the Age Periodization (Moscow, 1965). We conducted a morphological study of enamel and dentin of teeth with carious and non-carious lesions (increased erasability, cuneal defects), by obtaining chips and microsections of teeth, followed by examination of the number of enamel prisms, dentinal tubules,their diameter, the thickness of the gaps between these structures. The studies were carried out using a scanning electron microscope (SEM) "Mira 3 LMU" ("Tescan", Czech Republic) with a maximum resolution of 1 nm and a maximum magnification of 1,000,000. Dentally removed teeth were washed in running water, cleaned of plaque, dried by filter paper and crushed by vice, that was followed by the selection of the wreckage for the study. Then, markings in the dentine and enamel zones were made to fix the sizes of enamel prisms and dentinal tubules. RESULTS: Results: After the completion of the determination of enamel prisms and dentinal tubules diameter, counting of their number was carried out according to the method proposed by I.M. Tkachenko, by obtaining a series of digital images of the examined tooth's enamel and dentin with varying degrees of magnification. The number of prisms per unit area characterized the enamel density, on which permeability, hardness, tissue resistance to fracture, bending and modulus of elasticity depends. The number of dentinal tubules characterized the density of dentin and the peculiarities of trophic processes that occur in dentin. Also, in our opinion, the amount of dentinal tubules determines the damping properties of dentin and strength enamel values. CONCLUSION: Conclusions: Thus, the structure of enamel and dentin has peculiarities of the morphological structure at various clinical conditions of hard teeth tissues. This peculiarity can be associated with a decrease of enamel density, that is associated with a decrease in the number of prisms in the non-carious teeth pathology and an increase of the gaps between them.

Studying the skills attitudes on factors affecting dental health of children.

OBJECTIVE: Introduction: Nowadays, the high level of dental morbidity among children is an urgent medical problem. Healthy lifestyles and sanitary-hygienic upbringing in the family is most important components of the formation of dental health of children. But in order to be able to teach their children, parents need to be themselves knowledgeable about hygiene and prevention, have the necessary skills and desire to instill it in their children. The aim: The aim of our study was to assess the level of awareness of schoolchildren parents about the factors affecting the dental health of their children according to the questionnaire. PATIENTS AND METHODS: Materials and methods: The article presents the results of a sociological survey in the form of anonymous polls on a specially designed questionnaire. With the help of the sociological method, 408 questionnaires, which were filled up by parents of students of general school №19 in Poltava, were processed and analyzed. RESULTS: Results: Among the interviewed individuals, we investigated groups of predictors that could increase the parents' awareness of the factors influencing the formation of health; to teach children by parents of the rules of personal hygiene of oral cavity and skills of self-prevention of dental diseases; on factors that lead to proper orthodontic pathology. CONCLUSION: Conclusions: Based on the parents' answers to the factors which form the health of the population, we can conclude that their awareness on this issue is insufficient. The results of the survey show insufficient level of knowledge in the issues of the formation of health and prevention of dental diseases among the respondents. Thus, the necessity to carry out the corresponding work in educational institutions with the involvement of teachers, dental doctors, pediatricians and doctors of other specialties is determined.