Metagenomics reveals the resistance patterns of electrochemically treated erythromycin fermentation residue.

Erythromycin fermentation residue (EFR) represents a typical hazardous waste produced by the microbial pharmaceutical industry. Although electrolysis is promising for EFR disposal, its microbial threats remain unclear. Herein, metagenomics was coupled with the random forest technique to decipher the antibiotic resistance patterns of electrochemically treated EFR. Results showed that 95.75% of erythromycin could be removed in 2 hr. Electrolysis temporarily influenced EFR microbiota, where the relative abundances of Proteobacteria and Actinobacteria increased, while those of Fusobacteria, Firmicutes, and Bacteroidetes decreased. A total of 505 antibiotic resistance gene (ARG) subtypes encoding resistance to 21 antibiotic types and 150 mobile genetic elements (MGEs), mainly including plasmid (72) and transposase (52) were assembled in EFR. Significant linear regression models were identified among microbial richness, ARG subtypes, and MGE numbers (r2=0.50-0.81, p< 0.001). Physicochemical factors of EFR (Total nitrogen, total organic carbon, protein, and humus) regulated ARG and MGE assembly (%IncMSE value = 5.14-14.85). The core ARG, MGE, and microbe sets (93.08%-99.85%) successfully explained 89.71%-92.92% of total ARG and MGE abundances. Specifically, gene aph(3')-I, transposase tnpA, and Mycolicibacterium were the primary drivers of the resistance dissemination system. This study also proposes efficient resistance mitigation measures, and provides recommendations for future management of antibiotic fermentation residue.

Effects of deep coal mining on groundwater hydrodynamic and hydrochemical processes in a multi-aquifer system: Insights from a long-term study of mining areas in ecologically fragile western China.

The groundwater hydrodynamic and hydrochemical process of the multi-aquifer system will experience complicated and serious influence under deep coal mining disturbance. There is relatively little research that has integrated hydrodynamic and hydrochemical properties of groundwater to investigate the spatiotemporal distribution characteristics and evolution patterns of hydrogeochemistry and hydrodynamic information in deep multi-aquifer systems. The study of the groundwater hydrodynamic and hydrochemical spatiotemporal coupling response of multi-aquifer systems under the deep and special thick coal seam mining-motivated effect in ecologically fragile western mining areas is of great significance for the safe mining of coal resources and ecological environment protection. In this research, the hydrochemical analysis data composed of 218 groundwater samples from Tangjiahui coalfield, Northwest China with 1526 measurements and a 6-year (2016-2021) sampling period were collected for studying the hydrogeochemical spatiotemporal evolution process and governing mechanism of the multi-aquifer system using hierarchical cluster analysis, ion-ratio method, saturation index and multidimensional statistical analysis. Additionally, wavelet analysis and cross-wavelet coherence analysis were implemented to quantitatively recognize the spatiotemporal variation characteristics of hydrodynamic information and analyze the coherence relationships between time series. The results demonstrate that the hydrochemical characteristics exhibit significant spatial differences, while the temporal variation of hydrochemical characteristics in the Permian Shanxi Formation fractured sandstone aquifer (PSFFA), mine water (MW), and Ordovician karst limestone aquifer (OKA) is not significant. The water-rock interaction is the predominant control mechanism for the spatial evolution of hydrogeochemistry in the research area. Moreover, the large-scale mining of deep coal seams controls the type and degree of water-rock interactions by damaging the structure of aquifers and altering the hydrodynamic conditions of groundwater. The period from 2016 to 2021 exhibits multi-time scale characteristics in time series of precipitation, mine water discharge, and the water level of PSFFA and OKA. The mine water discharge has a positive correlation with the water level of PSFFA and OKA, whereas the significant period of precipitation and the water level of PSFFA coherence is not obvious. The research findings not only provide in-depth insights to protect the groundwater resources in water-shortage mining areas but also promote the secure mining of deep coal resources.

Erythromycin fermentation residue exposure induces a short-term wave of antibiotic resistance in a soil-lettuce system.

The pattern of antibiotic resistance assembly and their unclear transfer in a soil-lettuce system render the treated erythromycin fermentation residue (EFR) land application risky. Herein, the antibiotic resistance genes (ARGs), mobile gene elements, and microbial communities were examined under erythromycin stress at three stages of lettuce growth. Erythromycin exhibited degradation rates of 99.4 % in soils for 60 d, with little uptake in the seedling tissues, reaching a 0.11-0.71 bioconcentration factor range. The EFR application rate must be limited <1 % to avoid human exposure risk. The diversity, biotic networks complexity, and edaphic ARG abundances of the rhizospheric microbial communities increased at the early stage, but returned to the control levels at the mature stage. The Planomicrobium and Pseudomonas bacterial genera were important biotic factors for erythromycin variation. Thirty-three MLSB genes (macrolide, lincosamide, and streptogramin B) conferring resistance to erythromycin were detected in soil, but only two endophytic ARGs (mphA-01 and ermX) were identified, with members of the Microvirga genus being the potential hosts. Partial least-squares path modeling suggested that erythromycin concentration was the main factor for endophytic ARGs evolution. This study highlighted the leaf endophytic ARG emergence and potential exposure human risks majorly caused by the drug traces in antibiotic fermentation residues.

Sustainable on-farm strategy for the disposal of antibiotic fermentation residue: Co-benefits for resource recovery and resistance mitigation.

Antibiotic fermentation residue is a key issue for the sustainable operation of pharmaceutical companies, and its improper disposal may cause antibiotic resistance transfer in the environment. However, little is known about the resource recycling strategy of this pharmaceutical waste. Herein, we used hydrothermal spray-dried (HT+SD) and multi-plate dryer (MD) methods to produce bio-organic fertilizers and applied them to an internal recycling model of a field trial. The concentrations of antibiotics (penicillin, cephalosporin, and erythromycin) in the bio-fertilizer, wastewater, and exhaust gas were in the range of 0.002-0.68 mg/kg, ≤ 0.35 ng/mL, and 0.03-0.89 ng/mL, respectively. The organic matter and total nitrogen, phosphorus, and potassium contents were approximately 80% and 10%, respectively. The soil bacterial community was similar among the fertilizer treatments in the same crop cultivation. A total of 233 antibiotic resistance genes (ARGs) and 43 mobile genetic elements (MGEs) were detected, including seven Rank I ARGs and five Rank II ARGs. Random forest analysis showed that gene acc(3)-Via and plasmid trb-C were biomarkers, for which the resistance and the transfer mechanisms were antibiotic inactivation and conjugation, respectively. The results imply that AFR recycling disposal mode is a promising prospect for pharmaceutical waste management.