Immunopathology alters Th17 cell glucocorticoid sensitivity.

Th17 cells contribute to several inflammatory conditions and increasing evidence supports that Th17 cells are glucocorticoid resistant. However, Th17 cells in psoriasis and related diseases are glucocorticoid sensitive. We compare glucocorticoid sensitive and resistant immunological diseases and suggest that several aspects in Th17-related diseases alter glucocorticoid sensitivity of Th17 cells. We identify molecular pathways that are implicated in glucocorticoid sensitivity of Th17 cells in the literature, as this information is useful for developing approaches to overcome glucocorticoid-resistant immunopathology.

Identification of differentially expressed genes between high and low marbling score grades of the longissimus lumborum muscle in Hanwoo (Korean cattle).

We conducted DD-RT-PCR analysis to identify differentially expressed genes between high and low marbling score groups with extremely different IMF content of the longissimus lumborum muscles in Hanwoo. We detected 137 DEGs between two marbling score groups. Of these DEGs, 41 DEGs were highly expressed in the high marbling score group, whereas 96 DEGs showed a higher expression in the low marbling score group. Among them, we selected eight DEGs exhibiting the greatest differential expression levels between two marbling score groups. These genes could be classified as five candidate genes according to homology to known genes and biological functions. The expression of four DEGs except for ATP6 gene was confirmed by qRT-PCR. The TPI1 gene was significantly higher expressed at the high marbling group, whereas ACTA1, TNNT1 and MDH2 genes were significantly higher expressed at the low marbling group. Therefore, the TPI1 gene was up-regulated, suggesting that this gene may have an important function related to IMF deposition.

Pseudorhodobacter psychrotolerans sp. nov., a psychrotolerant bacterium isolated from terrestrial soil, and emended description of the genus Pseudorhodobacter.

A Gram-stain-negative, facultatively aerobic, cream-coloured, ovoid-shaped, non-motile and psychrotolerant bacterial strain, PAMC 27389T, was isolated from terrestrial soil collected on King George Island, Antarctica. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PAMC 27389T belongs to the genus Pseudorhodobacter, sharing highest similarities with the type strains of Pseudorhodobacter wandonensis (96.9 %), Pseudorhodobacter antarcticus (96.8 %), Pseudorhodobacter ferrugineus (96.5 %) and Pseudorhodobacter aquimaris (95.4 %). Average nucleotide identity values between strain PAMC 27389T and the type strains of P. wandonensis, P. antarcticus, P. ferrugineus and P. aquimaris were 70.8, 70.9, 71.0 and 70.5 %, respectively and the genome-to-genome distances were 18.4-19.1 %, indicating PAMC 27389T is clearly distinguished from the most closely related Pseudorhodobacter species. The genomic DNA G+C content was 60.1 mol%. Strain PAMC 27389T grew at 0-37 °C (optimally at 15-20 °C), at pH 5.5-9.0 (optimally at pH 6.5-7.0) and in the presence of 0.5-3.0 % (w/v) sea salt (optimally with 0.5 %). It lacked bacteriochlorophyll a. The major fatty acids (>5 %) were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C18 : 1ω7c 11-methyl. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid, an unidentified lipid and three unidentified aminophospholipids. The major respiratory quinone was Q-10. Based on the phenotypic, chemotaxonomic and genomic data presented, we propose the name Pseudorhodobacter psychrotolerans sp. nov. with the type strain PAMC 27389T ( = KCTC 42640T = JCM 30764T).

Comparison of alfuzosin 10 mg with or without propiverine 10 mg, 20 mg in men with lower urinary tract symptom and an overactive bladder: randomised, single-blind, prospective study.

PURPOSE: The efficacy and safety of treatment with alfuzosin 10 mg plus propiverine 10 or 20 mg in men with lower urinary tract symptoms (LUTS) and an overactive bladder were investigated. MATERIALS AND METHODS: In this parallel-arm, prospective, multicentre, single-blind study, men who were ≥ 40 years old, had an International Prostate Symptom Score (IPSS) of ≥ 8, an Overactive Bladder Symptom Score (OABSS) of ≥ 3 and an OABSS urgency item score of ≥ 2 were randomised in a 1 : 1 :1 ratio to receive alfuzosin 10 mg alone (Group A) or with propiverine 10 mg (Group B) or 20 mg (Group C) for 8 weeks. Four and 8 weeks after commencing treatment, OABSS was measured along with IPSS, maximal urinary flow rate (Qmax ) and postvoid residual volume (PVR). Adverse events were recorded. RESULTS: A total of 135 men, including 43 in Group A, 48 in Group B and 44 in Group C, completed the study. Relative to baseline, all groups demonstrated significant reductions in OABSS and the IPSS after eight treatment weeks (p < 0.005). The improvement of OABSS in Group C was significantly greater than Group A and B (Group A: 0.70 ± 1.94; Group B: 2.50 ± 2.98; Group C: 4.30 ± 3.40; p < 0.005). An observed improvement of Qmax and PVR in the three groups did not achieve statistical significance. Overall adverse event rates were higher in Group C but not significant compared with others. CONCLUSION: In patients with LUTS and overactive bladder, combined therapy with alfuzosin 10 mg plus propiverine 20 mg was significantly more effective than alfuzosin monotherapy and propiverine 10 mg combined therapy in terms of improving OABSS while not significantly affecting Qmax or PVR.

Development of inorganic and organic hybrid nanocoating based on carbon nanotubes for corrosion resistance.

In this study, we report the synthesis and characterization of novel hybrid nanocoating based on carbon nanotubes (CNTs) on anodized aluminum surfaces (AAO). The hybrid nanocoating was deposited by number of methods which include spray coating, spin coating and dip coating. The bonding of nanocoating with metal surface is an important parameter for successful modification of the metal surfaces. The improved adhesion of nanocoating on metal surfaces could be attributed to chemical bonding of sol-gel nanocoating with anodized surfaces. The nanocoated anodized aluminum surfaces showed superior adhesion and excellent anticorrosive properties. The nanocoated panels showed enhanced galvanic protection comparable to 80% of titanium metal as determined by galvanic corrosion measurements. It also showed higher thermal conductivities than stainless steel and bare anodized surfaces.

Selective glucocorticoid receptor translational isoforms reveal glucocorticoid-induced apoptotic transcriptomes.

Induction of T-cell apoptosis contributes to the anti-inflammatory and antineoplastic benefits of glucocorticoids. The glucocorticoid receptor (GR) translational isoforms have distinct proapoptotic activities in osteosarcoma cells. Here we determined whether GR isoforms selectively induce apoptosis in Jurkat T lymphoblastic leukemia cells. Jurkat cells stably expressing individual GR isoforms were generated and treated with vehicle or dexamethasone (DEX). DEX induced apoptosis in cells expressing the GR-A, -B, or -C, but not the GR-D, isoform. cDNA microarray analyses of cells sensitive (GR-C3) and insensitive (GR-D3) to DEX revealed glucocorticoid-induced proapoptotic transcriptomes. Genes that were regulated by the proapoptotic GR-C3, but not by the GR-D3, isoform likely contributed to glucocorticoid-induced apoptosis. The identified genes include those that are directly involved in apoptosis and those that facilitate cell killing. Chromatin immunoprecipitation assays demonstrated that distinct chromatin modification abilities may underlie the distinct functions of GR isoforms. Interestingly, all GR isoforms, including the GR-D3 isoform, suppressed mitogen-stimulated cytokines. Furthermore, the GR-C isoforms were selectively upregulated in mitogen-activated primary T cells and DEX treatment induced GR-C target genes in activated T cells. Cell-specific expressions and functions of GR isoforms may help to explain the tissue- and individual-selective actions of glucocorticoids and may provide a basis for developing improved glucocorticoids.

Enhanced Controlled Transdermal Delivery of Ambroxol from the EVA Matrix.

To avoid the systemic adverse effects that might occur after oral administration, transdermal delivery of ambroxol was studied as a method for maintaining proper blood levels for an extended period. Release of ambroxol according to concentration and temperature was determined, and permeation of drug through rat skin was studied using two chamber-diffusion cells. The solubility according to PEG 400 volume fraction was highest at 40% PEG 400. The rate of drug release from the EVA matrix increased with increased temperature and drug loading doses. A linear relationship existed between the release rate and the square root of loading rate. The activation energy (Ea) was measured from the slope of the plot of log P versus 1000/T and was found to be 10.71, 10.39, 10.33 and 9.87 kcal/mol for 2, 3, 4 and 5% loading dose from the EVA matrix, respectively. To increase the permeation rate of ambroxol across rat skin from the EVA matrix, various penetration enhancers such as fatty acids (saturated, unsaturated), propylene glycols, glycerides, pyrrolidones, and non-ionic surfactants were used. The enhancing effects of the incorporated enhancers on the skin permeation of ambroxol were evaluated using Franz diffusion cells fitted with intact excised rat skin at 37° using 40% PEG 400 solution as a receptor medium. Among the enhancers used, polyoxyethylene-2-oleyl ether increased the permeation rate by 4.25-fold. In conclusion, EVA matrix containing plasticizer and permeation enhancer could be developed for enhanced transdermal delivery of ambroxol.

Factors associated with participation in providing buccal cell DNA for a genetic epidemiologic study.

AIMS: Our aim was to investigate factors associated with participation in buccal cell DNA collection using a large-scale prospective cohort study. METHODS: Of the 183,634 Korea Medical Insurance Corporation (KMIC) prospective cohort study enrollees, 44,773 (36,794 men) were randomly selected. Buccal cell collection kits were mailed and collected from March to September 2004. Epidemiologic characteristics measured at baseline (1990 or 1992) and during follow-up (1998) were compared between respondents and nonrespondents. RESULTS: Among the 13,084 (29.2%) respondents, 78.7% submitted usable samples (DNA concentration ≥3 µg). After adjustments for either or both age and family history of chronic disease, participation in men was positively associated with old age, a family history of chronic disease, low subjective health status, and regular exercise, and negatively associated with current smoking status. In women, only old age was associated with participation. Low body mass index (BMI) (<18.5 kg/m(2)), blood pressure, total cholesterol, and fasting blood glucose level were negatively associated with participation in men after adjustments for age and family history of chronic disease, but not in women, except for low BMI. CONCLUSIONS: In this study, collecting buccal cell DNA by mailed survey has a low success rate (29%), and there appear to be a nonresponse bias and gender differences in the collection process.

First Report of Bursaphelenchus thailandae on Pinus densiflora in Korea.

A survey of nematodes on Pinus densiflora in Namyangju City, Gyeonggi Province, Korea was conducted in 2008. Namyangju is located in an area with pine wilt caused by Bursaphelenchus xylophilus and monitoring of wilt has been conducted periodically. Nematodes were extracted from wood chips by the Baermann's funnel method and were cultured on a medium of Botrytis cinerea. During the survey, B. xylophilus and B. thailandae were detected. B. thailandae was found in 20% of the trees tested and usually was found alone without B. xylophilus. Population density of B. thailandae ranged from 8,000 to 15,000 nematodes per 100 g of wood chips. The species was identified by morphology and molecular characterization. B. thailandae was differentiated from B. xylophilus by slight swellings of the stylet knobs, protruding vulval flaps and pointed tail in females, and the male spicule with distinctive shading in the dorsal region. Morphometric data was obtained by measurements of 20 females and 10 males: female, body length = 951.3 ± 56.6 µm, a = 31.8 ± 1.9, b = 10.8 ± 0.9, c = 23.4. ± 0.8, V = 76.2 ± 0.8, stylet length = 14.5 ± 0.6 µm, length of postuterine sac = 90.3 ± 13.5 µm; male, body length = 838.0 ± 71.5 µm, a = 31.1 ± 2.1, b = 5.2 ± 0.5, c = 30.4 ± 2.7, stylet length = 13.5 ± 1.1 µm, spicules length = 16.5 ± 0.7 µm. Measurements were compared with the original description of B. thailandae reported by Braasch and Braasch-Bidasak (1), which showed that the Korean isolate was larger in most body sizes. For molecular diagnosis, the internal transcribed spacer (ITS) and D2-D3 rDNA were amplified by PCR (US/PTC-0220; Bio Rad, Hercules, CA). The sequences of ITS and D2-D3 rDNA of B. thailandae from P. densiflora showed 98 and 99% homology to those of B. thailandae from GenBank Accession Nos. AM157746 and DQ497184, respectively. B. thailandae was originally described on Pinus merkusi in Pai, North Thailand (1) while Ningbo Entry-Exit Inspection and Quarantine Bureau in China reported a detection of B. thailandae in the imported packaging wood from Hong Kong, Japan, Korea, Italy, and the United States (2). However, packaging wood is a circulating product and the establishment of B. thailandae in those countries is not confirmed. The pathogenicity of B. thailandae on coniferous trees remains to be determined. To our knowledge, this is the first report of B. thailandae on P. densiflora in a natural area in Korea. References: (1) H. Braasch and R. Braasch-Bidasak. Nematology 4:853, 2002. (2) J. Gu et al. For. Pathol. 36:323, 2006.

Effects of baicalein, an antioxidant, on the bioavailability of doxorubicin in rats: possible role of P-glycoprotein inhibition by baicalein.

The purpose of this thesis is to investigate the effects of baicalein, an antioxidant, on the bioavailability and pharmacokinetics of Doxorubicin (DOX) in rats. Thus, DOX was administered intravenously (i.v.; 10 mg x kg(-1)) or orally (p.o.; 50 mg x kg(-1)) with or without oral baicalein (0.3, 1.5 and 6 mg x kg(-1)). In the presence of 1.5 and 6 mg x kg(-1) of baicalein, the total area under the plasma concentration-time curve from time zero to time infinity (AUC) and the peak concentration (Cmax) of DOX were significantly greater and higher, respectively, than those of the control. Consequently, the absolute bioavailability (AB) of DOX in the presence of baicalein was 3.5-4.4%, which was significantly enhanced compared with that of the control group (2.2%). The relative bioavailability (RB) of DOX was 1.20 to 1.96 times higher than that of the control group. Compared to the intravenous control, the presence of oral baicalein increased the i.v. AUC of DOX and other pharmacokinetic parameters were not significantly affected. The enhanced bioavailability of oral DOX by oral baicalein may be due to the inhibition of both P-glycoprotein (P-gp) and the cytochrome P450 (CYP) 3A subfamily by baicalein in the intestine and/or liver. This result suggests that the development of oral DOX is feasible by combination with baicalein that would be more convenient than the i.v. dosage forms. Furthermore, since the present study raises the awareness about potential drug interactions by concomitant use of DOX with baicalein, the dosage regimen of DOX should be taken into consideration, if this result is confirmed in clinical studies.

First Report of Bursaphelenchus doui on Tulip Tree (Liriodendron tulipifera) in Korea.

Tulip tree (Liriodendron tulipifera) is one the major tree species in Korean plantations, but it originated from North America. Bursaphelenchus doui was collected from the wood of dead tulip trees located at Wanju in Chonnam Province, Korea. Nematodes were maintained in fungal medium culture (Botrytis cinerae) on potato dextrose agar. B. doui is morphologically close to B. xylophilus, a causative organism of pine wilt disease, and identification to species is difficult. However, B. doui differs from the other species of the xyophilus group by the larger spicule (33.8 to 43.3 µm) (1). For accurate species identification, both morphology and molecular biological methods were applied. Morphological observations showed lip region 3.7 µm high and offset by a constriction, median bulb well developed, esophageal gland overlapping intestine dorsally, vulval flap present, tail terminus with distinct mucro. Males ventrally curved by fixation, spicules pared, and large with small, cucullus, oval-shaped bursa. Measurements of 20 females and 10 males (female; body length = 901.7 ± 60.5 µm, a = 34.3 ± 3.1, b = 10.4 ± 1.5, c = 23.0 ± 2.3, V = 77.1 ± 2.0, stylet length = 14.3 ± 0.4 µm, tail length = 39.5 ± 4.2 µm, length of postuterine sac = 104.6 ± 7.8: male; body length = 790.4 ± 40.6 µm, a = 34.4 ± 2.7, b = 10.8 ± 1.7, c = 22.7 ± 1.3, stylet length = 14.3 ± 0.3 µm, tail length = 34.8 ± 1.9 µm, spicules length = 39.5 ± 4.1 µm) were close to the original description of B. doui by Braasch et al. (1). For molecular diagnosis, DNA was extracted from more than 30 individual nematodes with a DNeasy Kit (Qiagen, Valencia, CA) and the internal transgenic spacers (ITS) 1, 2, and 5.8S regions in rDNA were amplified by PCR (US/PTC-0220; Bio Rad, Hercules, CA). ITS-restriction fragment length polymorphism (RFLP) can discriminate closely related species by using five different restriction enzymes (2). ITS-RFLP patterns obtained with these enzymes were the same as reported by Braasch et al. (1). B. doui previously has been detected in Korean wooden packages imported to China (1). However, most production of Korean wooden packages depends on imported coniferous trees from other countries. Although B. doui has been reported in Japanese red pine (Pinus densiflora) in Japan (3), the natural distribution of B. doui in Korea is unclear. On the basis of morphological and molecular biological information, we identify this nematode as B. doui. To our knowledge, this is the first report of B. doui on tulip tree and in a natural area in Korea. References: (1) H. Braasch et al. Russ. J. Nematol. 12:19, 2004. (2) W. Burgermeister et al. Russ. J. Nematol. 13:29, 2005. (3) N. Kanzaki et al. Nematology. 10:69, 2008.

First Report of Bursaphelenchus pinophilus on Korean pine (Pinus koraiensis).

The genus Bursaphelenchus Fuchs, 1937 contains approximately 90 species (3) that are morphologically similar. Pine wood nematode, Bursaphelenchus xylophilus (4) Nickle, 1970, is the causal organism of pine wilt disease and accurate identification is essential for diagnosis of the disease. In Korea, pine wilt disease was first reported in 1988 and devastated 6,800 ha of pine forest through 2008. For a survey of trees with pine wilt disease, wood samples were taken randomly from dead Pinus koraiensis in Namyangju, Gyeonggi Province in Korea. The extracted nematodes from dead trees were maintained in culture on Botrytis cinerea and morphological characteristics were observed with an inverted light microscope (Leica DE/DMI 3000B). Identification of Bursaphelenchus spp. based on morphological characteristics is difficult, especially for identification of juveniles that carry few morphological features for species identification. The internal transcribed spacer (ITS) region in ribosomal DNA provides useful molecular diagnostic markers for this genus (1). The nematodes were provisionally identified as Bursaphelenchus pinophilus based on the characteristic long and arcuate body shape, male spicule with distinctive rostrum and small cucullus, female vulval flap, and mucronate conical tail. Other Bursaphelenchus spp. with vulval flaps and spicules with cucullus are B. xylophius, B. mucronatus, B. abruptus, and B. pinophilus. For molecular diagnosis, DNA was extracted from more than 30 individual nematodes with a DNeasy Kit (Qiagen, Valencia, CA) and ITS regions 1, 2, and 5.8S in rDNA were amplified by PCR (US/PTC-0220; Bio Rad, Hercules, CA). The ITS-restriction fragment length polymorphism pattern was consistent with that of B. pinophilus (2). The ITS rDNA sequence of B. pinophilus from Korean pines had a 98% sequence homology to that of B. pinophilus in GenBank (Accession No. AM160664). The pathogenicity of B. pinophilus has not been determined. To our knowledge, this is the first report of B. pinophilus on P. koraiensis, but it was previously reported from Poland, Germany, and Portugal on P. sylvestris and P. pinaster (1). References: (1) H. Braasch. EPPO Bull. 31:127, 2001. (2) W. Burgermeister et al. Russ. J. Nematol. 13:29, 2005. (3) R. Sriwati et al. Nematology 10:1, 2008. (4) G. Steiner and E. M. Buhrer. J. Agric. Res. 48:946, 1934.

Scaling behavior of the first arrival time of a random-walking magnetic domain.

We report a universal scaling behavior of the first arrival time of a traveling magnetic domain wall into a finite space-time observation window of a magneto-optical microscope enabling direct visualization of a Barkhausen avalanche in real time. The first arrival time of the traveling magnetic domain wall exhibits a nontrivial fluctuation and its statistical distribution is described by universal power-law scaling with scaling exponents of 1.34+/-0.07 for CoCr and CoCrPt films, despite their quite different domain evolution patterns. Numerical simulation of the first arrival time with an assumption that the magnetic domain wall traveled as a random walker well matches our experimentally observed scaling behavior, providing an experimental support for the random-walking model of traveling magnetic domain walls.

Comparison of conductive fabric sensor and Ag-AgCI sensor under motion artifacts.

A wearable electrocardiogram(ECG) device using conductive fabric sensor was compared with traditional Ag-AgCl electrode ECG device. The ECG signals were measured under existence of motion artifacts on variable running speed using treadmill to verify that wearable device can substitute traditional ECG device. A signal to noise ratio (SNR) and RR interval were compared between the two devices. The SNR of wearable device was similar or higher than that of clinical device and difference of RR interval was 2ms. The results show that the wearable ECG device using conductive fabric sensor can make similar performance with ECG device using Ag-AgCl electrode even under motion artifacts.

First Report of the European Type of Bursaphelenchus mucronatus on Korean Pine (Pinus koraiensis) in Korea.

More than 70 species of Bursaphelenchus have been reported and they are morphologically very similar. The pine wood nematode (Bursaphelenchus xylophilus) is a causal agent of pine wilt and accurate identification is essential for diagnosis of the disease. However, many other saprophytic nematodes are found in dead trees and some of them have high morphological similarity to B. xylophilus. B. mucronatus is commonly found in dead pine trees and is distinguished from B. xylophilus only by the presence of a distinct mucro in the female tail (3). However, natural populations of B. xylophilus show variation in tail morphology and some of them have a small lump on the end of the tail that looks like a short mucro, which could lead to misidentification. B. mucronatus has been reported to have two genotypes known as the East Asian type and the European type (2). The European type has a more rounded tail with a mucro, which is much more similar to B. xylophilus than the East Asian type. Molecular identification by internal transcribed spacer restriction fragment length polymorphism (ITS-RFLP) can distinguish these two genotypes clearly by using five different restriction enzymes (2). In Korea, we collected wood samples from dead Pinus koraiensis located at Pocheon, Gyeonggi Province in April, 2007 and nematodes were extracted by a modified Baermann funnel method. After 24 h, nematodes were collected and morphology was observed under an inverted light microscope (Leica DE/DMI 3000B, Wetzlar, Germany). Morphology was characterized by a typical Aphelenchoid-type esophagus, head constriction, female vulva flap, post uterine sac, female tail with mucro, and males with arched-shaped spicules. The tail was rounded with a long mucro >4 µm. For molecular diagnosis, DNA was extracted from single individual nematodes by using a DNeasy Kit (Qiagen, Valencia, CA) and the ITS 1 and 2 and 5.8S regions in rDNA were amplified by PCR (US/PTC-0220; Bio-Rad, Hercules, CA). ITS-RFLP patterns were analyzed and nucleotide sequences were compared with sequences of B. mucronatus already reported in GenBank (Accession Nos. AM179514, BMU93554, and DQ841162). The ITS-RFLP pattern of individuals matched the European type of B. mucronatus and was differentiated from the East Asian type by RsaI and HaeIII digestion (2). The ITS DNA sequence of nematodes from Korean pines was >98% identical to B. mucronatus in GenBank. The European type of B. mucronatus was consistently isolated from dead trees, but the pathogenicity of the European type of B. mucronatus on Korean pine is not yet clear. To our knowledge, this is the first report of the European type of B. mucronatus species on P. koraiensis in Korea, but it is already distributed in certain locations of Japan (4), China, and Europe (1). References: (1) H. Braasch et al. Nachrbl. Dtsch. Pflanzenschutzd. 51:312, 1999. (2) W. K. Burgermeister et al. Russ. J. Nematol. 13:29, 2005. (3) Y. Mamiya and N. Enda. Nematologica 25:353, 1979. (4) K. Togashi et al. Jpn. J. For. Res. 13:127, 2008.

First Report of Pine Wilt Disease on Pinus koraiensis in Korea.

Pine wilt disease is one of the most important forest tree diseases, especially in the East Asian countries of Japan, China, and Korea. The causal agent, Bursaphelenchus xylophilus (3), is transmitted by the insect vectors, pine sawyer beetles (Monochamus spp.). The vectors mainly attack coniferous trees and the infected trees die within several weeks. In Korea, pine wilt disease was first reported in Busan City in 1988, and now, the damaged area covers 7,820 ha and more than 60 cities in Korea. The main host trees are Pinus densiflora and P. thunbergii, which are the most common pines distributed in Korea. In 2006, however, we found pine wilt disease in a forest of P. koraiensis located in Gwangju City in Gyeonggi Province. Symptoms were systemic and leaves turned brown or yellowish. Wood samples were collected from the affected trees, and nematodes were extracted by a modified Baermann's funnel method. After 24 h, nematodes were collected from wood chips, and morphology was observed with an inverted light microscope (Leica DE/DMI 3000B, Wetzlar, Germany). Morphology was characterized by a typical Aphelenchoid-type esophagus, head constriction, female vulva flap, female tail, and a male spicule shape similar to B. xylophilus. For more accurate identification, DNA was extracted from individual nematodes with a DNeasy Kit (Qiagen, Valencia, CA). The internal transcribed spacers (ITS) 1 and 2 and 5.8S regions were amplified by PCR (1). Sequenced nucleotide information was compared with the sequences of B. xylophilus already reported in GenBank (Accession Nos. AB294736, AB277208, AM 157747, AY 347913, and BXU92464). ITS DNA sequences of the nematode from Korean pine was >99% identical to B. xylophilus in GenBank. The B. xylophilus from Korean pine tree was also successfully cultured in Botrytis cinerea medium and pathogenicity was tested from June to October 2007. More than 95% mortality was observed with the inoculation of 20 replicate 15-year-old trees of P. koraiensis and P. densiflora with 15,000 nematodes per tree. Therefore, we confirmed that pine wilt disease occurred in P. koraiensis in Korea. P. koraiensis is an endemic species in Korea and distribution is limited to the northern Korean Peninsula and some locations in Russia. It has been shown that P. koraiensis is susceptible to the pine wood nematode by an inoculation test in Japan (2); however, to our knowledge, this is the first report of pine wilt disease on P. koraiensis under field conditions in Korea. References: (1) W. K. Burgermeister et al. Russ. J. Nematol. 13:29, 2005. (2) K. Futai and T. Furuno. Bull. Kyoto Univ. For. 51:3, 1979. (3) G. Steiner and E. M. Buhrer. J. Agr. Res. 48:946, 1934.

Expression and characterization of a recombinant Cry1Ac crystal protein with enhanced green fluorescent protein in acrystalliferous Bacillus thuringiensis.

AIMS: To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS: The N-terminal fusion expression of EGFP in Cry1Ac was attempted under the control of the native cry1Ac promoter. The EGFP gene was cloned into pProMu and named pProMu-EGFP. The transformant, ProMu-EGFP/CB produced parasporal inclusions that were of bipyramidal-shaped crystals in size ranging from 200 to 300 nm. The fusion protein was approximately 150 kDa and identified by the immunoblot analysis using a Cry1Ac antibody and also a GFP antibody. The LC(50) of the ProMu-EGFP/CB was twofold higher when compared with that by the ProAc/CB. However, the crystal protein produced by the ProMu-EGFP/CB was effective on Plutella xylostella larvae. CONCLUSIONS: The ProMu-EGFP/CB produced bipyramidal shaped and insecticidal crystals comprising fusion proteins. SIGNIFICANCE AND IMPACT OF THE STUDY: Through the N-terminal fusion expression of EGFP and Cry1Ac, expression and crystallization between the B. thuringiensis crystal protein and a foreign protein were validated.

Realization of an e-health system to perceive emergency situations.

Emergency situations can occur anywhere and anytime in daily life. In the paper, we present an e-health system to perceive emergency situations of a patient. Using a wearable shirt (BioShirt) and a personal monitoring system (PBM), we obtain the body signals of a user. The monitoring system collects and transmits the vital signs to a personal digital assistant (PDA) via a BlueTooth communication module. To detect emergency from the received data, a simple detection algorithm is performed in the PDA. And the PDA forwards the data to an e-health central monitoring room (ECMR), if necessary. In the ECMR, several operators supervise the registered users based on incoming body signals from each user's device. If an automatic decision-making algorithm generates an emergency alarm, the operators try to contact the corresponding patient and recognize his status. Ultimately if they decide that the patient is an urgent situation, they give phone calls and messages to the emergency center and the patient's medical attendant immediately.

5-HT2A receptor gene promoter polymorphism -1438A/G and bipolar disorder.

Genetic factors, such as the genes involved in the serotonin pathway, probably play an important role in the pathogenesis of bipolar disorder, and serotonin type 2A (5-HT2A) receptor gene promoter polymorphism -1438A/G has been reported. This study investigated the association between -1438A/G polymorphism of 5-HT2A receptor gene promoter and bipolar disorder in a Korean population. Using the polymerase chain reaction, -1438A/G polymorphism typed in 142 patients with bipolar disorder and in 148 normal control subjects. Differences in genotype distributions and allele frequencies of -1438A/G between patients with bipolar disorder and normal control subjects were tested for significance using the chi-squared test. There were significant differences in genotype distributions [chi2 = 9.697, degrees of freedom (df) = 2, P = 0.008] and allele frequencies (chi2 = 7.284, df = 1, P = 0.007) of -1438A/G between patients with bipolar disorder and normal control subjects. Although further studies are necessary, these results in a Korean population suggest that -1438A/G polymorphism of 5-HT2A receptor gene promoter may be causally related to the development of bipolar disorder.