ABSTRACT
The present work was designed to study the specific features of 2-methyl hydroxybezene and 3-methyl hydroxybenzene distribution after intragastric administration of these toxicants to warm-blooded animals (rats). They were detected in the unmetabolized form in the internal organs and blood of the animals. The levels of 2-methyl hydroxybezene were especially high in the stomach and blood whereas the maximum content of 3-methyl hydroxybenzene was found in brain, blood, small intestines of the poisoned rats.
Subject(s)
Brain/metabolism , Forensic Toxicology/methods , Intestine, Small/chemistry , Phenol/pharmacokinetics , Animals , Brain/drug effects , Brain Chemistry , Chromatography , Disease Models, Animal , Intestine, Small/drug effects , Intestine, Small/metabolism , Phenol/toxicity , Rats , Spectrophotometry, UltravioletABSTRACT
Optimal conditions have been determined for furadan isolation from biological fluids by means of the mixture of solvents ethyl acetate-acetone in volume 1:1. Possible purification of the compound from coextractive substances of biomaterial on the column with silica gel L 100/160 mcm is demonstrated. IR-spectrophotometric methods and high performace liquid chromatography with a detector of photodiode matrix are proposed for identification and quantitation of furadan in blood and urine specimens.
