ABSTRACT
Objetivo: Se llevó a cabo un estudio de corte transversal con el objetivo de evaluar la utilidad de la ecografía Doppler con índice de resistencia (IR) en comparación con la gammagrafía renal con 99mTc-DTPA en niños con estenosis ureteropiélica unilateral. Métodos: El estudio incluyó niños menores de 15 años con diagnóstico de estenosis ureteropiélica unilateral, riñón contralateral sano con o sin antecedente de plastia ureteropiélica. Los pacientes seleccionados se derivaron al Departamento de Medicina Nuclear para llevar a cabo la gammagrafía renal y días más tarde se derivaron al Departamento de Radiología para la realización de la ecografía Doppler renal con IR. Resultados: Un total de 21 pacientes fueron incluidos en el estudio, 15 hombres y 6 mujeres, que representan el 71,4% y el 28,6%, respectivamente. La edad promedio fue de 5,3 años. Solo 3 tuvieron un antecedente de plastia ureteropiélica en el riñón afectado. La gammagrafía renal informó datos de obstrucción unilateral en 18 pacientes, incluidos los 3 pacientes con cirugía previa. La tasa promedio de filtración glomerular (TFG) en general obtenida con la gammagrafía fue de 100,28 ml/min. El promedio de TFG fue de 43,03 ml/min en los riñones afectados y de 57,24 ml/min en los riñones sanos (p < 0,001). La ecografía Doppler con IR reportó ectasia en el 100% de los riñones afectados y en un riñón contralateral normal. El IR promedio fue de 0,69 mm/s en los riñones afectados y de 0,50 mm/s en los riñones sanos (p < 0,001). Conclusiones: Con los resultados obtenidos, podemos sugerir que la ecografía Doppler renal con medición del IR puede ser una herramienta alternativa a la gammagrafía renal con 99mTc-DTPA en algunos casos
Objective: A cross-sectional study was carried out with the objective of evaluating the usefulness of Doppler ultrasound with resistive index (RI) measure compared with renal scintigraphy with 99mTc-DTPA in children with unilateral ureteropelvic junction obstruction. Methods: The study included children under 15 years with a diagnosis of unilateral ureteropelvic junction obstruction, healthy contralateral kidney with or without an antecedent of ureteropyeloplasty. The selected patients were sent to the Nuclear Medicine Department to carry out a renal scintigraphy with 99mTc-DTPA and days later were sent to the Radiology Department for the performance of Renal Doppler Ultrasound with RI. Results: A total of 21 patients were included in the study, 15 males and 6 females, representing 71.4% and 28.6%, respectively. Mean age was 5.3 years. Only 3 had an antecedent of ureteropyeloplasty in the affected kidney. The scintigraphy reported data of unilateral obstruction in 18 patients, including the 3 patients with previous surgery. The average glomerular filtration rate (GFR) obtained with the scintigraphy was 100.28 ml/min. The average GFR in affected kidneys was 43.03 ml/min and 57.24 ml/min in healthy kidneys (p < .001). Doppler ultrasound with RI reported ectasia in 100% of the affected kidneys and one normal contralateral kidney. The average RI in affected kidneys was 0.69 mm/s and 0.50 mm/s in healthy kidneys (p < .001). Conclusions: With the results obtained, we can suggest that ultrasound Doppler with measurement of RI can be an alternative tool to renal scintigraphy with 99mTc-DTPA in some cases
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Urethral Stricture/diagnostic imaging , Tomography, Emission-Computed, Single-Photon , Echocardiography, Doppler , Radionuclide Imaging , Cross-Sectional Studies , Radiopharmaceuticals , Sensitivity and SpecificityABSTRACT
OBJECTIVE: A cross-sectional study was carried out with the objective of evaluating the usefulness of Doppler ultrasound with resistive index (RI) measure compared with renal scintigraphy with 99mTc-DTPA in children with unilateral ureteropelvic junction obstruction. METHODS: The study included children under 15 years with a diagnosis of unilateral ureteropelvic junction obstruction, healthy contralateral kidney with or without an antecedent of ureteropyeloplasty. The selected patients were sent to the Nuclear Medicine Department to carry out a renal scintigraphy with 99mTc-DTPA and days later were sent to the Radiology Department for the performance of Renal Doppler Ultrasound with RI. RESULTS: A total of 21 patients were included in the study, 15 males and 6 females, representing 71.4% and 28.6%, respectively. Mean age was 5.3 years. Only 3 had an antecedent of ureteropyeloplasty in the affected kidney. The scintigraphy reported data of unilateral obstruction in 18 patients, including the 3 patients with previous surgery. The average glomerular filtration rate (GFR) obtained with the scintigraphy was 100.28ml/min. The average GFR in affected kidneys was 43.03ml/min and 57.24ml/min in healthy kidneys (p<.001). Doppler ultrasound with RI reported ectasia in 100% of the affected kidneys and one normal contralateral kidney. The average RI in affected kidneys was 0.69mm/s and 0.50mm/s in healthy kidneys (p<.001). CONCLUSIONS: With the results obtained, we can suggest that ultrasound Doppler with measurement of RI can be an alternative tool to renal scintigraphy with 99mTc-DTPA in some cases.
Subject(s)
Kidney Pelvis/diagnostic imaging , Kidney/diagnostic imaging , Radiopharmaceuticals , Technetium Tc 99m Pentetate , Ultrasonography, Doppler , Ureteral Obstruction/diagnostic imaging , Child, Preschool , Cross-Sectional Studies , Female , Humans , Male , Radionuclide ImagingABSTRACT
The use of radioactive emanations has been of great importance for the performance of endourology procedures, such as percutaneous nephrolithotomy (NLP). The damage to health caused by radiation has been a sensitive issue. The objective of this work was to determine the dose received by the surgeon during NLP and the total dose generated by the fluoroscope. A cross-sectional study was conducted with data from a cohort study with a duration of 18 months that included 101 patients. Radiation was measured with dosimeter during the last 6 months. During the last 6 months of the study, 34 patients were submitted to surgery. The average age was 47 years. Average fluoroscopy time was 58.3 second (24-122 seconds) in both male and female groups, with 57.16 seconds and 58.95 seconds per case, respectively (P = .6). Radiation emitted during 6 months for the 34 patients was 330.5 mGy. The total radiation measured by the dosimeter was 1 mSv, which is equivalent to 0.3% of the total radiation applied during the procedures. Doses measured by the dosimeter on the surgeon were within the recommended annual doses although dose received by the hands exceeds the authorized limits (500 mSv/y).
ABSTRACT
BACKGROUND: Transcription factor STAT3 has a prominent innate immunity effect on cancer progression. We determined the regulation of STAT3 in the immunophenotype modulation of macrophages from M1 into M2 induced by the cell-culture supernatant of the Prostate-Cancer line PC3. METHODS: Monocytes-macrophages from healthy donors were cultured in the supernatant of PC3 cells, membrane proteins, and intracytoplasmic and phosphorylated STAT3 were measured using flow cytometry, while cytokines and growth factors were studied using luminescence. Cytotoxicity and nitric oxide were evaluated via colorimetric assays. RESULTS: The supernatant of PC3 prostate-tumor cells effectively induced macrophages toward an M2 profile, and the expression of phosphorylated STAT3 in the monocytes-macrophages notably increased, and mainly related to IL-10. In the group of monocytes-macrophages treated with a STAT3 inhibitor, the macrophages were induced toward an M1 phenotype. CONCLUSIONS: In this study, we showed that the secretion profile of PC3 prostate-cancer cells induces a change in macrophage phenotype from M1 into M2, and that the phenomenon is related to phosphorylation of transcription factor STAT3 and IL-10.