Different sources of dietary n-6 polyunsaturated fatty acids and their effects on antibody responses in chickens.

1. Effects of linoleic and linolenic acid provided via different oil sources on total antibody (Ab) titres, Ab isotypes after primary and secondary immunisation, and cutaneous hypersensitivity (CH) responses to bovine serum albumin (BSA) and maleyl-BSA, respectively, were studied in pullets fed on one of 4 diets. The diets were the basal control diet enriched with either sunflower oil or safflower oil as sources of linoleic acid, and linseed oil as a source of linolenic acid, tested against a control diet supplemented with animal fat. 2. Total Ab and immunoglobulin (Ig) isotype responses to BSA were affected by diet after primary, and diet x immunisation effects after secondary immunisation. Higher total Ab and IgG titres to BSA were found especially after primary immunisation in birds given the sunflower oil enriched diet, whereas birds given sunflower oil mounted significantly lower IgM titres to BSA after primary and secondary immunisation. The antibody responses to maleyl-BSA were affected by diet after primary, and immunisation x diet interactions after secondary immunisation. Sunflower oil enhanced total and IgG Ab titres to maleyl-BSA after primary immunisation, but decreased IgM titres to maleyl-BSA after primary and secondary immunisation. Cutaneous hypersensitivity responses to BSA and maleyl-BSA were not affected by the diet. 3. It is concluded that modulation of the magnitude and isotype of Ab responses of poultry to T cell-dependent antigens is affected not only by type of essential fatty acids, but also by their source. In the present study the n-6 source, sunflower oil, showed strong enhancement of primary Ab responses, directed to both Th2 and Th1 antigens. On the other hand, the different effects of safflower oil imply that constituents other than n-6 acids within dietary plant oils may affect immune responsiveness. 4. The relationship between magnitude and isotype of Ab responsiveness, type of antigen, and essential fatty acids is discussed.

Interactions of dietary polyunsaturated fatty acids and vitamin E with regard to vitamin E status, fat composition and antibody responsiveness in layer hens.

1. Effects of dietary polyunsaturated fatty acids (PUFA) and vitamin E (VE) on an immune response may interact because VE may protect PUFA from in vivo oxidation. The present study was designed to study the presence of such an interaction in growing layer chickens. 2. Three dietary concentration of linoleic acid (LA, 3.3, 6.6 and 10%), in combination with 4 concentration of dietary VE (5, 20, 40 and 80 mg/kg) were used. Effects of LA and VE on circulating VE concentration, fatty acid composition of bursal and adipose fat, and antibody kinetics against keyhole limpet hemocyanin and Mycobacterim butyricum were established. 3. At high dietary LA concentration, bursal and adipose LA were higher but bursal arachidonic acid and long chain n-3 PUFA decreased. The dietary VE level did not consistently affect the deposition of PUFA in tissue. Plasma VE concentrations were affected by the dietary VE and LA content, but not by their interaction. Antibody responses before and 7 d after immunisation were affected by the dietary treatments. Antibody concentration were not affected by tissue fatty acid content. 4. In conclusion, the interaction effects of dietary PUFA and VE on fat deposition and immune responses are of minor importance compared to separate PUFA and VE effects. This implies that, within the studied range, adding extra VE to preserve or affect the effects of dietary PUFA on antibody responsiveness is unnecessary.