ABSTRACT
A new water-soluble heteropolysaccharide (PS-II) with apparent molecular weight â¼1.65×10(5) Da, was isolated from the fruiting bodies of hybrid mushroom pfle 1p by hot aqueous extraction. It is composed of d-mannose, d-galactose, and 3-O-Me-d-galactose in a molar ratio of 1.0:0.99:1.1. The structural investigation of PS-II has been carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and 1D/2D NMR experiments. Based on the results of these experiments, it was established that PS-II contained a main chain of (1â6) linked α-d-galactopyranosyl residues, one of which was substituted at C-2 by a terminal mannopyranosyl residue and also methylated at C-3 position. This heteropolysaccharide (PS-II) exhibited macrophage activation by NO production as well as in vitro splenocyte and thymocyte stimulation.
Subject(s)
Fruiting Bodies, Fungal/chemistry , Fungal Polysaccharides/chemistry , Galactans/chemistry , Pleurotus/chemistry , Shiitake Mushrooms/chemistry , Carbohydrate Sequence , Chimera/genetics , Fungal Polysaccharides/isolation & purification , Fungal Polysaccharides/pharmacology , Galactans/isolation & purification , Galactans/pharmacology , Galactose/chemistry , Macrophage Activation/drug effects , Macrophages/cytology , Macrophages/drug effects , Mannose/chemistry , Molecular Sequence Data , Molecular Weight , Monocytes/cytology , Monocytes/drug effects , Pleurotus/genetics , Shiitake Mushrooms/genetics , Thymocytes/cytology , Thymocytes/drug effectsABSTRACT
A water-soluble heteropolysaccharide (PS-I) having molecular weight ~2.1×10(5) Da was isolated from hot aqueous extract of the fruit bodies of hybrid mushroom pfle 1p. The hybrid mushroom pfle 1p was obtained through intergenic protoplast fusion between Pleurotus florida and Lentinula edodes. The heteropolysaccharide contained D-glucose, D-galactose, and D-mannose in a molar ratio of nearly 4:2:1. The structural investigation of PS-I has been carried out using sugar and methylation analyses as well as 1D/2D NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Based on the results of these experiments, the repeating unit of the PS-I was established as: [structure: see text]. PS-I showed in vitro macrophage activation by NO production and also stimulated splenocytes and thymocytes.
Subject(s)
Pleurotus/chemistry , Polysaccharides/chemistry , Polysaccharides/immunology , Shiitake Mushrooms/chemistry , Animals , Carbohydrate Conformation , Macrophages/immunology , Mice , Nitric Oxide/biosynthesis , Spleen/immunology , Structure-Activity Relationship , Thymocytes/immunologyABSTRACT
An immunostimulating water-soluble heteroglycan (PS-II) was isolated from an aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h produced by intergeneric protoplast fusion between Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-II was carried out using sugar analysis, methylation experiment, periodate oxidation and 1D/2D NMR studies. Sugar analysis indicated the presence of glucose, mannose and galactose in a molar ratio of 1:1:2. Methylation analysis revealed that PS-II was composed of (1â6)- and (1â2,4,6)-α-D-galactopyranosyl, terminal ß-D-mannopyranosyl and terminal ß-D-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. On the basis of chemical analysis and NMR studies, the structure of the repeating unit of the heteroglycan was established to consist of a backbone chain of two (1â6)-α-D-galactopyranosyl residues, one of which is branched at O-2 with terminal ß-D-Manp and at O-4 with terminal ß-D-Glcp. This heteroglycan (PS-II) showed in vitro splenocyte, thymocyte and macrophage activations.
Subject(s)
Fruiting Bodies, Fungal/chemistry , Immunologic Factors/chemistry , Lentinula/chemistry , Pleurotus/chemistry , Polysaccharides/chemistry , Animals , Cells, Cultured , Chimera/genetics , Chimera/metabolism , Crosses, Genetic , Galactose/chemistry , Glucose/chemistry , Immunologic Factors/isolation & purification , Immunologic Factors/pharmacology , Lentinula/genetics , Macrophages/cytology , Macrophages/drug effects , Macrophages/immunology , Mannose/chemistry , Mice , Pleurotus/genetics , Polysaccharides/isolation & purification , Polysaccharides/pharmacology , Solubility , Thymocytes/cytology , Thymocytes/drug effects , Thymocytes/immunology , WaterABSTRACT
A water soluble polysaccharide isolated from the alkaline extract of the somatic hybrid mushroom (PfloVv5FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains was found to contain d-glucose only. Structural investigation was carried out using acid hydrolysis, methylation analysis; periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This polysaccharide exhibited strong immunoactivation of macrophages, splenocytes as well as thymocytes.
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Hybridization, Genetic , Pleurotus/chemistry , Volvariella/chemistry , beta-Glucans/chemistry , beta-Glucans/pharmacology , Adjuvants, Immunologic/isolation & purification , Animals , Carbohydrate Sequence , Hydrogen-Ion Concentration , Macrophages, Peritoneal/drug effects , Macrophages, Peritoneal/metabolism , Mice , Molecular Sequence Data , Pleurotus/genetics , Spleen/immunology , Thymocytes/drug effects , Thymocytes/immunology , Volvariella/genetics , beta-Glucans/isolation & purificationABSTRACT
A water soluble heteroglycan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes consists of d-glucose, d-mannose, and d-galactose residues in a molar ratio of nearly 1:1:1. This polysaccharide showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar hydrolysis, methylation, periodate oxidation study, and NMR studies (1H, 13C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as: -->6)-α-D-Galp-(1-->6)-α-D-Glcp-(1-->2<--1ß-D-Manp.
Subject(s)
Chimera , Pleurotus/chemistry , Polysaccharides/chemistry , Shiitake Mushrooms/chemistry , Carbohydrate Sequence , Magnetic Resonance SpectroscopyABSTRACT
A water soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid mushroom PCH3FB, obtained through protoplast fusion between the strains Pleurotus florida and Calocybe indica var. APK2. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as: [formula, see text] This molecule showed macrophage, splenocyte and thymocyte activations.
Subject(s)
Fungal Polysaccharides/chemistry , Fungal Polysaccharides/pharmacology , Macrophage Activation/drug effects , Macrophages/immunology , Pleurotus/chemistry , Thymocytes/immunology , Animals , Carbohydrate Conformation , Cells, Cultured , Macrophages/cytology , Mice , Thymocytes/cytologyABSTRACT
A water-soluble glucan (PS-I) was isolated from the aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-I was carried out using total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Methylation analysis revealed that PS-I was composed of (1â3, 6), (1â3), (1â6)-linked and terminal ß-d-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. The repeating unit of the glucan consists of a backbone chain of two (1â6)-ß-d-glucopyranosyl residues, one of which is branched at O-3 position with (1â3)-ß-d-glucopyranosyl and terminated with a ß-d-glucopyranosyl residue. Study of immunological activity revealed that PS-I stimulates the splenocytes, thymocytes and macrophages.
Subject(s)
Agaricales/chemistry , Fruiting Bodies, Fungal/chemistry , Fungal Polysaccharides/chemistry , Immunologic Factors/chemistry , Animals , Carbohydrate Conformation , Carbohydrate Sequence , Cell Line , Cell Proliferation/drug effects , Cell Survival , Fungal Polysaccharides/isolation & purification , Fungal Polysaccharides/pharmacology , Immunologic Factors/isolation & purification , Immunologic Factors/pharmacology , Macrophages/drug effects , Macrophages/immunology , Macrophages/physiology , Methylation , Mice , Molecular Sequence Data , Nitric Oxide/metabolism , Oxidation-Reduction , Periodic Acid/chemistry , Spleen/cytology , Thymocytes/drug effects , Thymocytes/immunology , Thymocytes/physiology , Thymus Gland/cytologyABSTRACT
An immunostimulating water-insoluble ß-glucan isolated from hot alkaline extract of the fruiting bodies of an edible somatic hybrid mushroom of Pleurotus florida and Calocybe indica var. APK2 showed significant macrophage, splenocyte, and thymocyte activations. On the basis of total hydrolysis, methylation analysis, and NMR experiments ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, DEPT-135, and HSQC), the repeating unit of the polysaccharide is established.
Subject(s)
Agaricales/chemistry , Agaricales/genetics , Macrophages, Peritoneal/drug effects , beta-Glucans/metabolism , beta-Glucans/pharmacology , Animals , Carbohydrate Conformation , Carbohydrate Metabolism , Cells, Cultured , Fruiting Bodies, Fungal/chemistry , Magnetic Resonance Spectroscopy , Mice , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effects , beta-Glucans/chemistryABSTRACT
A water soluble glucan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes showed macrophages, splenocytes, and thymocytes activation. The glucan consists of terminal, (1â3,6)-linked, and (1â6)-linked ß-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:3. On the basis of acid hydrolysis, methylation, periodate oxidation study, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the glucan was established as: [structure: see text].
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Glucans/chemistry , Glucans/isolation & purification , Pleurotus/chemistry , Shiitake Mushrooms/chemistry , Animals , Carbohydrate Sequence , Cell Line , Cell Proliferation/drug effects , Cells, Cultured , Macrophages/cytology , Macrophages/drug effects , Magnetic Resonance Spectroscopy , Mice , Molecular Sequence Data , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effectsABSTRACT
A water soluble heteroglycan, isolated from the alkaline extract of the fruit bodies of the somatic hybrid mushroom (PfloVv1aFB), raised through protoplast fusion between the strains of Pleorutus florida and Volverilla volvacea, was found to consist of (1â3)-, (1â6)-, (1â3,4)-linked, and terminal ß-D-Glcp along with (1â2,6)-α-D-Galp and terminal α-D-Manp in a relative proportion of approximately 1:1:1:1:1:1. This polysaccharide exhibited strong immunostimulating activity of macrophages as well as splenocytes and thymocytes. Structural investigation was carried out using sugar analysis, methylation analysis; periodate oxidation study, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: [formula see text].
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Pleurotus/chemistry , Polysaccharides/chemistry , Polysaccharides/immunology , Volvariella/chemistry , Adjuvants, Immunologic/isolation & purification , Alkalies/chemistry , Animals , Carbohydrate Conformation , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Fruit/chemistry , Macrophages/drug effects , Macrophages/immunology , Mice , Pleurotus/immunology , Polysaccharides/isolation & purification , Reactive Oxygen Species/immunology , Spleen/cytology , Spleen/drug effects , Spleen/immunology , Structure-Activity Relationship , Thymocytes/drug effects , Thymocytes/immunology , Volvariella/immunologyABSTRACT
Two different glucans (PS-I and PS-II) were isolated from the alkaline extract of the fruiting bodies of an edible mushroom, Pleurotus florida blue variant and the PS-I showed macrophage, splenocyte and thymocyte activations. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of these polysaccharides were established.
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Glucans/chemistry , Glucans/pharmacology , Pigmentation , Pleurotus/chemistry , Adjuvants, Immunologic/isolation & purification , Carbohydrate Sequence , Glucans/isolation & purification , Macrophages/drug effects , Macrophages/immunology , Molecular Sequence Data , Solubility , Spleen/drug effects , Spleen/immunology , Thymocytes/drug effects , Thymocytes/immunology , Water/chemistryABSTRACT
An immunostimulating water-soluble glucan was isolated from hot aqueous extract of fruit bodies of an edible mushroom Calocybe indica. Structural investigation of the glucan was carried out using acid hydrolysis, methylation analysis, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as [see figure in text]. This glucan stimulated the splenocytes and thymocytes.
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Agaricales/chemistry , Glucans/chemistry , Glucans/pharmacology , Adjuvants, Immunologic/isolation & purification , Animals , Carbohydrate Sequence , Cell Proliferation/drug effects , Glucans/isolation & purification , Mice , Molecular Sequence Data , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effectsABSTRACT
Two different glucans (water-soluble PS-I, water-insoluble PS-II) were isolated from the alkaline extract of the fruit bodies of hybrid mushroom. PS-I was found to consist of only (1â6)-linked ß-D-glucopyranose. PS-II was composed of terminal, (1â3,4)-linked, and (1â3)-linked ß-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:1. PS-I showed macrophages, splenocytes, and thymocytes activation as well as antioxidant property. On the basis of sugar analysis, methylation analysis, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these glucans were established as:
Subject(s)
Glucans/chemistry , Glucans/pharmacology , Hybridization, Genetic , Pleurotus/chemistry , Pleurotus/genetics , Volvariella/chemistry , Volvariella/genetics , Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/isolation & purification , Adjuvants, Immunologic/pharmacology , Animals , Antioxidants/chemistry , Antioxidants/isolation & purification , Antioxidants/pharmacology , Biphenyl Compounds/chemistry , Carbohydrate Sequence , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Glucans/isolation & purification , Hydrogen-Ion Concentration , Macrophages, Peritoneal/drug effects , Macrophages, Peritoneal/immunology , Mice , Molecular Sequence Data , Picrates/chemistry , Solubility , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effects , Water/chemistryABSTRACT
Three polysaccharide fractions (PS-I, PS-II, and PS-III) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-ß glucan. PS-II and PS-III were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as;
Subject(s)
Chimera/metabolism , Galactans/metabolism , Glucans/metabolism , Pleurotus , Volvariella , Carbohydrate Conformation , Carbohydrate Sequence , Chimera/genetics , Galactans/chemistry , Galactans/isolation & purification , Glucans/chemistry , Glucans/isolation & purification , Glucose/chemistry , Inbreeding , Magnetic Resonance Spectroscopy , Mannose/chemistry , Molecular Sequence DataABSTRACT
A water-soluble polysaccharide of an edible mushroom Calocybe indica var. APK2 showed immunoenhancing (macrophage, splenocyte, thymocyte, and bone marrow activation) and cytotoxic activity toward HeLa cell lines and found to consist of D-glucose, D-galactose, and L-fucose in a molar ratio of nearly 3:1:1. On the basis of acid hydrolysis, methylation analysis, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the fuco-galacto-glucan was established as: [see formula in text].
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Agaricales/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Polysaccharides/chemistry , Polysaccharides/pharmacology , Adjuvants, Immunologic/isolation & purification , Animals , Antineoplastic Agents/isolation & purification , Carbohydrate Sequence , Cell Proliferation/drug effects , HeLa Cells , Humans , Magnetic Resonance Spectroscopy , Mice , Molecular Sequence Data , NIH 3T3 Cells , Polysaccharides/isolation & purificationABSTRACT
A water soluble polysaccharide isolated from the hot aqueous extract of the fruit bodies of the somatic hybrid mushroom (PfloVv1aFB), raised through protoplast fusion between the strains of Pleurotus florida and Volvariella volvacea was found to consist of d-glucose, d-galactose, and d-mannose in a molar ratio of nearly 4:1:1 and showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as: [structure: see text].
Subject(s)
Agaricales/chemistry , Pleurotus/growth & development , Polysaccharides/chemistry , Polysaccharides/pharmacology , Volvariella/growth & development , Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Animals , Carbohydrate Sequence , Cell Proliferation/drug effects , Cells, Cultured , Chimera , Galactose/chemistry , Glucose/chemistry , Macrophages/cytology , Macrophages/drug effects , Magnetic Resonance Spectroscopy , Mannose/chemistry , Mice , Thymocytes/cytology , Thymocytes/drug effectsABSTRACT
A water-soluble glucan, isolated from the alkaline extract of the fruit bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to consist of (1â3,6)-linked, (1â3)-linked, (1â6)-linked, and terminal ß-d-glucopyranosyl moieties in a relative proportion of approximately 1:2:1:1. This polysaccharide showed optimum activation of macrophages as well as splenocytes and thymocytes at 10µg/mL. Structural investigation was carried out using sugar analysis, methylation analysis, periodate oxidation study, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as:
Subject(s)
Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/isolation & purification , Agaricales/chemistry , Glucans/chemistry , Glucans/isolation & purification , Lentinula/chemistry , Adjuvants, Immunologic/pharmacology , Animals , Carbohydrate Sequence , Cell Proliferation/drug effects , Cells, Cultured , Glucans/pharmacology , Macrophages/cytology , Macrophages/drug effects , Magnetic Resonance Spectroscopy , Mice , Molecular Sequence Data , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effectsABSTRACT
A water-soluble glucan isolated from an alkaline extract of fruit bodies of a somatic hybrid mushroom PCH9FB of Pleurotus florida and Calocybe indica var. APK2 strains showed antioxidant properties with immune activation of macrophage, splenocyte, and thymocyte. On the basis of acid hydrolysis, methylation, periodate oxidation and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structure of the repeating unit of the glucan was established as: [structure: see text].
Subject(s)
Alkalies/chemistry , Antioxidants/pharmacology , Chemistry Techniques, Analytical/methods , Glucans/immunology , Glucans/isolation & purification , Hybridization, Genetic , Pleurotus/chemistry , Animals , Cell Proliferation/drug effects , Glucans/chemical synthesis , Glucans/pharmacology , Macrophage Activation/drug effects , Macrophages, Peritoneal/cytology , Macrophages, Peritoneal/drug effects , Magnetic Resonance Spectroscopy , Mice , Nitric Oxide/pharmacology , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effectsABSTRACT
A water-soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid PCH9FB, obtained through intergeneric protoplast fusion between the strains Pleurotus florida and Calocybe indica var. On the basis of total acid hydrolysis, the polysaccharide was found to contain galactose, fucose, and glucose in a molar ratio of nearly 2:1:2. Methylation analysis and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) showed that the structure of the repeating unit present in the polysaccharide was This molecule showed macrophage, splenocyte, thymocyte activation as well as antioxidant property.
Subject(s)
Agaricales/chemistry , Antioxidants/metabolism , Hybridization, Genetic , Pleurotus/chemistry , Polysaccharides/chemistry , Polysaccharides/immunology , Water/chemistry , Animals , Cell Proliferation , Macrophage Activation , Macrophages, Peritoneal/cytology , Macrophages, Peritoneal/metabolism , Magnetic Resonance Spectroscopy , Mice , Nitric Oxide/metabolism , Polysaccharides/isolation & purification , Spleen/cytology , Thymus Gland/cytologyABSTRACT
Two different glucans (PS-I, water-soluble; and PS-II, water-insoluble) were isolated from the alkaline extract of fruit bodies of an edible mushroom Calocybe indica. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR analysis ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these polysaccharides were established as: PS-I: â6)-ß-D-Glcp-(1â6)-ß-D-glcp-(1â6)-)-ß-D-Glcp-(1â α-D=Glcp (Water-soluble glucan). PS-II: â3)-ß-D-Glcp-(1â3)-ß-D-glcp-(1â3)-)-ß-D-Glcp-(1â3)-ß-D-Glcp-(1â ß-D-Glcp (Water-insoluble glucan, Calocyban).