Effect of Seaweed-Derived Fucoidans from Undaria pinnatifida and Fucus vesiculosus on Coagulant, Proteolytic, and Phospholipase A2 Activities of Snake Bothrops jararaca, B. jararacussu, and B. neuwiedi Venom.

BACKGROUND: Snakebite envenomation (SBE) causes diverse toxic effects in humans, including disability and death. Current antivenom therapies effectively prevent death but fail to block local tissue damage, leading to an increase in the severity of envenomation; thus, seeking alternative treatments is crucial. METHODS: This study analyzed the potential of two fucoidan sulfated polysaccharides extracted from brown seaweeds Fucus vesiculosus (FVF) and Undaria pinnatifida (UPF) against the fibrinogen or plasma coagulation, proteolytic, and phospholipase A2 (PLA2) activities of Bothrops jararaca, B. jararacussu, and B. neuwiedi venom. The toxicity of FVF and UPF was assessed by the hemocompatibility test. RESULTS: FVF and UPF did not lyse human red blood cells. FVF and UPF inhibited the proteolytic activity of Bothrops jararaca, B. jararacussu, and B. neuwiedi venom by approximately 25%, 50%, and 75%, respectively, while all venoms led to a 20% inhibition of PLA2 activity. UPF and FVF delayed plasma coagulation caused by the venoms of B. jararaca and B. neuwiedi but did not affect the activity of B. jararacussu venom. FVF and UPF blocked the coagulation of fibrinogen induced by all these Bothropic venoms. CONCLUSION: FVF and UPF may be of importance as adjuvants for SBE caused by species of Bothrops, which are the most medically relevant snakebite incidents in South America, especially Brazil.

Purple Carrot Extract Exhibits a Neuroprotective Profile in th e Nigrostriatal Pathway in the Reserpine-induced Model of Parkinson 's Disease.

BACKGROUND: Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway. Even with scientific and technological advances, the therapeutic approaches used for the treatment of PD have shown to be largely ineffective in controlling the progression of symptoms in the long term. There is a growing demand for the development of novel therapeutic strategies for PD treatment. Different herbs and supplements have been considered as adjuvant to treat the symptoms of Parkinsonism. The carrot is one of the most consumed vegetable species worldwide, and its root is known for its content of anthocyanins, which possess antioxidant and antiinflammatory properties. This study evaluated the neuroprotective effect of purple carrot extract (CAR) in rats on the reserpine (RES)-induced progressive parkinsonism model. METHODS: Male rats (6-month-old) received orally the CAR (400 mg/kg) or vehicle and subcutaneously RES (0.01 mg/kg) or vehicle for 28 days (Preventive Phase). From the 29th day, rats received CAR or vehicle daily and RES (0.1 mg/kg) or vehicle every other day (for 23 days, Protective phase). Behavioral tests were conducted throughout the treatment. Upon completion, the animals' brain were processed for tyrosine hydroxylase (TH) immunohistochemical assessment. RESULTS: Our results showed that the chronic treatment of CAR protected against motor disabilities, reducing the time of catalepsy behavior and decreasing the frequency of oral movements, possibly by preserving TH levels in the Ventral Tegmental Area (VTA) and SNpc. CONCLUSION: CAR extract is effective to attenuate motor symptoms in rats associated with increased TH+ levels in the Ventral Tegmental Area (VTA) and SNpc, indicating the potential nutraceutical benefits of CAR extract in a progressive parkinsonism model induced by RES.

Bone Resorption in Apical Periodontitis Enhanced by Cigarette Smoke Inhalation: Histometric, Immunohistochemical, and Microtomographic Analysis in Rats.

INTRODUCTION: This study evaluated the effects of cigarette smoke inhalation (CSI) on apical periodontitis (AP) induced in rats by histometric, immunohistochemical, and microtomographic analysis. METHODS: A total of 32 male Wistar rats were divided into 4 experimental groups (n = 8): control, CSI, AP, and CSI + AP. Rats in the CSI and CSI + AP groups inhaled cigarette smoke by remaining inside a smoking chamber for 8 minutes 3 times a day for 50 days. After 20 days of smoke inhalation, rats in the AP and CSI + AP groups had the pulp of their first right lower molar exposed to induce AP. Blood was collected on day 50 to evaluate nicotine and serum cotinine levels. The animals' mandibles were removed for histologic processing to evaluate bone resorption by histometric, immunohistochemical (receptor activator of nuclear factor kappa B ligand/osteoprotegerin), and microtomographic analysis. The Student t test was applied. RESULTS: Histometric analysis showed a larger area of bone resorption (P < .05) and microtomographic analysis found greater resorption volume (P < .001) for the CSI + AP group compared with the AP group. The CSI + AP group presented a high RANKL immunostaining pattern compared with the AP group (P < .001). CONCLUSIONS: CSI increased bone resorption caused by AP.

Effects of cigarette smoke inhalation on the immune-inflammatory profile of experimental apical periodontitis in rats.

OBJECTIVE: To evaluate the effects of cigarette smoke inhalation on the immune-inflammatory profile of experimental apical periodontitis in rats. METHODOLOGY: In total, 32 male Wistar rats were divided into four groups (n = 8): AP-induced apical periodontitis; S-cigarette smoke inhalation; APS-induced AP and cigarette smoke inhalation; and C (control)-neither AP nor cigarette smoke inhalation. To induce cigarette smoke inhalation, the animals were kept in a chamber filled with tobacco smoke for 8 min thrice a day for 50 days. AP was induced 20 days after inhalation initiation by exposing their coronary pulp to their oral environment for 30 days. After animals were euthanized, their right hemimaxillae were removed for histopathological, semi-quantitative and immunohistochemical (F4/80, CD206 and iNOS) analyses. RESULTS: Quantitative data showed a moderate number of inflammatory infiltrates in AP and an intense number in APS (p < .05). Comparing F4/80+ cells showed no statistically significant differences among groups, but we found more CD206+ cells in AP than in C and S (p > .05). INOS+ immunostaining showed a significant increase in AP and APS, when compared with C and S (p < .05). APS had more iNOS+ cells than AP (p < .05). CONCLUSION: Cigarette smoke inhalation worsened AP, leading to a predominantly pro- inflammatory profile in our experimental model.

Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing.

Biocompatibility and biomineralization of root canal dressings are important requirements for periapical healing. This study evaluated the inflammatory response, biomineralization and tissue repair by collagen fiber maturation in the subcutaneous tissue of rats. Eighteen Wistar rats (n = 6) received subcutaneous implants: calcium hydroxide + propylene glycol [CH+P], calcium hydroxide + propylene glycol + iodoform [CH+P+I], iodoform + carbowax [I+Cwax] and carbowax [Cwax]. Extra empty tubes were used as a control [C]. After 7, 15 and 30 days, the implants were removed with surrounding tissue for staining of hematoxylin-eosin, Von Kossa, picrosirius red and without staining for analysis under polarized light. Results were analyzed via Kruskal-Wallis followed by Dunn testing for nonparametric data and ANOVA followed by a Tukey post hoc test for parametric data (p < 5%). At 7 days, all groups showed a moderate inflammatory reaction and thick fibrous capsule, except the [Cwax] group, with a severe inflammatory infiltrate (p < 0.05). After 15 days, all groups but control had a decrease in inflammatory response. At 30 days, all groups presented a mild reaction and thin fibrous capsule (p > 0.05). Only groups containing calcium hydroxide were found to be positive using Von Kossa staining and polarized light in all periods. At 7 days, all groups showed a higher proportion of immature fibers. At 15 days, the [CH+P] and [Cwax] groups increased their proportion of mature/immature fibers. At 30 days, only the [CH+P] group presented a significant prevalence of mature collagen fibers (p < 0.05). All groups showed biocompatibility, but only groups containing calcium hydroxide induced biomineralization. The addition of iodoform delayed tissue healing.

Subcutaneous, Oral, and Intranasal Immunization of BALB/c Mice with Leishmania infantum K39 Antigen Induces Non-Protective Humoral Immune Response.

Visceral leishmaniasis is a high-burden disease caused by parasites of the Leishmania genus. The K39 kinesin is a highly antigenic protein of Leishmania infantum, but little is known about the immune response elicited by this antigen. We evaluated the humoral immune response of female BALB/c mice (n = 6) immunized with the rK39-HFBI construct, formed by the fusion of the K39 antigen to a hydrophobin partner. The rK39-HFBI construct was administered through subcutaneous, oral, and intranasal routes using saponin as an adjuvant. We analyzed the kinetics of IgG, IgG1, and IgG2a production. The groups were then challenged by an intravenous infection with L. infantum promastigote cells. The rK39-HFBI antigen-induced high levels of total IgG (p < 0.05) in all groups, but only the subcutaneous route was associated with increased production of IgG1 and IgG2a 42 days after immunization (p < 0.05), suggesting a potential secondary immune response following the booster dose. There was no reduction in the splenic parasite load; thus, the rK39-HFBI failed to protect the mice against infection under the tested conditions. The results presented here demonstrate that the high antigenicity of the K39 antigen does not contribute to a protective immune response against visceral leishmaniasis.

Virtual screening indicates potential inhibitors of the P2X7 receptor.

Anti-inflammatory agents can be synthetic or natural compounds and are often used to attenuate different levels of inflammation. Inflammatory diseases, due to the involvement of multiple systems, are becoming difficult to treat, involve long durations of therapy where applicable, have a high cost of management and have a deleterious impact on public health. The search for natural and synthetic compounds with anti-inflammatory activity is an important strategy in drug design. Bioactive synthetic drugs may be repurposed for other pharmacological applications, and natural product chemical structures offer unlimited opportunities for new drug discoveries due to the unparalleled availability of chemical diversity. Virtual screening of 2774 molecules on the mouse P2X7 protein showed that potential ligands are composed of five flavonoids (narirutin, diosmin, complanatuside, hesperidin, and oroxin B) and other drugs such as velpatasvir, itacitinib and lifitegrast. In vitro studies in mouse cells confirmed the inhibitory activity of the indicated ligands on the P2X7 receptor by applying virtual screening. The behavior of protein bonded to the ligands was verified by analysis of the molecular dynamic simulation trajectories for four of the most potent inhibitor compounds, indicating that the ligands velpatasvir, itacitinib, lithospermic acid and narirutin remained in the binding site indicated by molecular docking.

Effect of Enterococcus faecalis Biofilm on Corrosion Kinetics in Titanium Grade 4 Alloys with Different Surface Treatments.

E. faecalis has been associated with bacteremia, sepsis, and bacterial endocarditis and peri-implantitis. This microorganism can remain in the alveolus even after extraction of the root remnant. This study aimed to evaluate the corrosion on different surfaces of commercially pure titanium (Ti) grade 4 (Ticp-G4) as a function of the bacterial biofilm effect of Enterococcus faecalis. A total of 57 discs were randomly divided according to their surface finish (n = 19). For microbiological analysis (n = 9), the discs were placed in 12-well plates containing E. faecalis culture and incubated at 37 °C for 7 days. The results show that for the intergroup analysis, considering the "electrolyte" factor, there was a difference between the groups. There was greater biofilm formation for the D.A.Zir group, with greater electrochemical exchange for Biofilm, and the presence of biofilm favored greater electrochemical exchange with the medium.

Biological investigation of resinous endodontic sealers containing calcium hydroxide.

The purpose of this study was to evaluate, in vivo, the biocompatibility, biomineralization, collagen maturation and the in vitro antibacterial and cytotoxicity of resinous endodontic sealers containing calcium hydroxide. Forty rats were implanted with polyethylene tubes containing Sealer 26, Sealer Plus, Dia-ProSeal and an empty tube, examined after 7, 15, 30 and 60 days. Antimicrobial activity was evaluated against Enterococcus faecalis by Agar Diffusion Test (ADT) through inhibition zones. For cytotoxicity, undifferentiated pulp cells (OD-21) were cultured and assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, exposed to dilution of serial extracts at 6, 24, 48h. Cytotoxicity was analyzed by two-way ANOVA and Bonferroni correction. Kruskal-Wallis test followed by Dunn test was performed for nonparametric data (p<0.05). MTT assay revealed cell proliferation affected by sealers extract in all periods (p<0.0001), except for Dia-Proseal and Sealer Plus ⅛ dilution. Subcutaneous analysis showed at day 7th moderate inflammatory infiltration. After 30 days, Sealer 26 still showed moderate inflammatory infiltrate compared to mild inflammation from control and Dia-ProSeal (p = 0.006). At day 60th, all groups showed similar mild inflammatory infiltrate (p>0.05). Sealer 26 induced more biomineralization than other sealers in all periods. At 7 and 15 days, all sealers had significant percentage of immature collagen fibers. After 60 days Sealer 26 showed more mature fibers compared to other sealers (p<0.001). All sealers had a smaller zone of inhibition than chlorhexidine, but with no significant difference among any group (p>0.05). All sealers showed satisfactory biological responses with in vitro/in vivo biocompatibility and antimicrobial activity against planktonic bacteria. Sealer 26 induced more biomineralization than Sealer Plus and Dia-ProSeal.

Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits.

BACKGROUND: This study aimed to evaluate peri-implant bone formation in rabbits after sinus grafting mediated by hydroxyapatite and beta-tricalcium phosphate (HA + ß-TCP) in granule or paste configurations, concomitant with immediate implant installation. MATERIAL & METHODS: Thirty-four rabbit maxillary sinuses were grafted with HA + ß-TCP, half of which were applied in a granule and half in a paste composition. Implant placement was performed simultaneously. At 7 and 40 days postoperatively, the animals were euthanized, and samples were prepared for tomographic, microtomographic, histological, histometric (hematoxylin and eosin staining, HE), and immunohistochemical (labeling of transcription factor Runx-2 [RUNX2], vascular endothelial growth factor [VEGF], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analysis. Implant removal torque was also measured. RESULTS: On tomography, maintenance of sinus membrane integrity was observed in both the groups. Higher values of morphometric parameters evaluated by micro-CT were found in the "paste group" after seven days. At 40 days, there were no significant differences between the groups in most of the microtomographic parameters evaluated. In histological sections stained with HE, a higher percentage of newly formed bone was observed in the "granule group" after 40 days. Similar positive immunolabeling was observed for both RUNX2 and OCN in both the experimental groups. TRAP immunolabeling was similar in both groups as well. VEGF labeling increased in the "granule group", indicating a higher osteoconductive potential in this biomaterial. Similar removal torque values were observed in both groups. Thus, the two HA + ß-TCP configurations showed similar healing patterns of simultaneously installed implants adjacent to sinus floor elevation. However, significantly higher bone values were observed for the "granule configuration". CONCLUSIONS: The HA + ß-TCP granules and paste presentations showed favorable long-term healing results, with bone formation in similar quantities and quality adjacent to the implants.

Percepções e Vivências de Psicólogos/as sobre a Punição Física em Crianças / Perceptions and Experiences of Psychologists about Physical Punishment in Children / Percepciones y Experiencias de los Psicólogos/as sobre el Castigo Físico en Niños/as

Este estudo qualitativo e transversal investigou as percepções e vivências dos/as psicólogos/as sobre o uso da punição física em crianças. Participaram do estudo 13 psicólogos/as brasileiros/as, que responderam a um questionário sociodemográfico on-line, um roteiro de entrevista e vinhetas de situação-problema. A análise de conteúdo revelou duas categorias: Ambivalência nas percepções sobre o uso da punição física: A palmada não é uma forma de agressão?; e Reprodução da punição física geração após geração: a forma de educar mudou?. Constataram-se percepções endossando o uso da punição física e experiências pessoais de punição física na infância, mas vários participantes buscam não repetir com os filhos e são desfavoráveis a todos os níveis dessa prática. Participantes favoráveis ao uso, principalmente da palmada, demonstraram desconhecimento da sua ineficácia enquanto método educativo e de seus efeitos prejudiciais. A transgeracionalidade e a naturalização da palmada foram motivos salientados para a persistência do uso da punição física. São necessárias capacitações a psicólogos/as e estudantes sobre os prejuízos e alternativas à punição física em crianças.

This qualitative and transversal work aimed to investigate the perceptions and experiences of psychologists about the use of physical punishment in children. Thirteen Brazilian psychologists participated in the study, who answered a socio-demographic questionnaire, an interview script and problem-situation vignettes online. Content analysis revealed two categories: Ambivalence in perceptions about the use of physical punishment: Isn't spanking a form of aggression? and Reproduction of physical punishment generation after generation: has the way of educating changed? Perceptions endorsing the use of physical punishment and personal experiences of physical punishment in childhood were found, but several participants try not to repeat it with their children and are unfavorable to all levels of this practice. Participants favorable to the use, mainly of spanking, demonstrated ignorance of its ineffectiveness as an educational method and its harmful effects. The transgenerationality and the naturalization of spanking were highlighted reasons for the persistence of the use of physical punishment. Training is needed for psychologists and students on the harm and alternatives to physical punishment in children.

Este estudio cualitativo y transversal tuvo como objetivo investigar las percepciones y experiencias de psicólogos sobre el uso del castigo físico en niños. Participaron del estudio trece psicólogos brasileños, que respondieron un cuestionario sociodemográfico, un guión de entrevista y viñetas de situaciones-problema en línea. El análisis de contenido reveló dos categorías: Ambivalencia en las percepciones sobre el uso del castigo físico: ¿Las nalgadas no son una forma de agresión? y Reproducción del castigo físico generación tras generación: ¿ha cambiado la forma de educar? Se encontraron percepciones que avalan el uso del castigo físico y experiencias personales del castigo físico en la infancia, pero varios participantes intentan no repetirlo con sus hijos y son desfavorables a todos los niveles de esta práctica. Los participantes favorables al uso, principalmente de las nalgadas, demostraron desconocer su ineficacia como método educativo y sus efectos nocivos. La transgeneracionalidad y la naturalización de las nalgadas fueron razones destacadas para la persistencia del uso del castigo físico. Se necesita capacitación para psicólogos y estudiantes sobre el daño y las alternativas al castigo físico en los niños.

In silico and pharmacological study of N,S-acetal juglone derivatives as inhibitors of the P2X7 receptor-promoted in vitro and in vivo inflammatory response.

Purinergic receptors are transmembrane proteins responsive to extracellular nucleotides and are expressed by several cell types throughout the human body. Among all identified subtypes, the P2×7 receptor has emerged as a relevant target for the treatment of inflammatory disease. Several clinical trials have been conducted to evaluate the effectiveness of P2×7R antagonists. However, to date, no selective antagonist has reached clinical use. In this work, we report the pharmacological evaluation of eleven N, S-acetal juglone derivatives as P2×7R inhibitors. Using in vitro assays and in vivo experimental models, we identified one derivative with promising inhibitory activity and low toxicity. Our in silico studies indicate that the 1,4-naphthoquinone moiety might be a valuable molecular scaffold for the development of novel P2×7R antagonists, as suggested by our previous studies.

Seroprevalence of Toxoplasmosis in Puerperal Women Treated at a Tertiary Referral Hospital.

OBJECTIVE: To evaluate the seroprevalence of toxoplasmosis among puerperal women cared for at a tertiary university hospital and the level of understanding of these puerperal women about toxoplasmosis, vertical transmission, and its prophylaxis. METHODS: For this cross-sectional study, we evaluated 225 patients using presential interviews, prenatal documentation, and electronic medical records. Data were stored using Research Electronic Data Capture (REDCap) software. Prevalence rates were estimated by the presence of reactive IgG antibodies against Toxoplasma gondii. Data analysis was performed using the chi-square test and calculation of the odds ratio (OR). Seroreactivity to T. gondii and exposure variables (age, educational level, and parity) were analyzed using a confidence interval (95%CI) and a significance level of 5% (p < 0.05). RESULTS: The seropositivity rate for T. gondii was 40%. There was no association between seroprevalence and age. Primiparity was a protective factor against seropositivity and low education was a risk factor. CONCLUSION: Knowledge of T. gondii infection and its transmission forms was significantly limited, presenting a risk for acute maternal toxoplasmosis and vertical transmission of this protozoan. Increasing the education level regarding the risk of toxoplasmosis during pregnancy could reduce the rates of infection and vertical transmission of this parasite.

OBJETIVO: Avaliar a soroprevalência de toxoplasmose entre puérperas atendidas em um hospital universitário terciário e o nível de compreensão dessas puérperas sobre toxoplasmose, transmissão vertical e sua profilaxia. MéTODOS: Para esse estudo transversal, foram avaliadas 225 pacientes utilizando entrevistas presenciais, documentação de pré-natal e prontuário eletrônico. Os dados foram armazenados usando o software Research Electronic Data Capture (REDCap). As taxas de prevalência foram estimadas pela presença de anticorpos IgG reativos contra o Toxoplasma gondii. A análise dos dados foi realizada por meio do teste do qui-quadrado e cálculo do odds ratio (OR). A sororreatividade ao T. gondii e as variáveis de exposição (idade, escolaridade e paridade) foram analisadas, utilizando-se intervalo de confiança (IC95%) e nível de significância de 5% (p < 0,05). RESULTADOS: A taxa de soropositividade para T. gondii foi de 40%. Não houve associação entre soroprevalência e idade. A primiparidade foi fator de proteção contra a soropositividade e a baixa escolaridade foi fator de risco. CONCLUSãO: O conhecimento da infecção por T. gondii e suas formas de transmissão foi significativamente limitado, apresentando risco para toxoplasmose materna aguda e transmissão vertical desse protozoário. Aumentar o nível de escolaridade quanto ao risco de toxoplasmose durante a gravidez pode reduzir as taxas de infecção e transmissão vertical desse parasita.

Polydioxanone Membrane Compared with Collagen Membrane for Bone Regeneration.

Guided bone regeneration (GBR) is an approach that induces osteopromotion through the regenerative membranes. These barriers exhibit bioactive behavior and mechanical function. Polydioxanone is a synthetic option, already used in medicine and dentistry, with good results in bone regeneration. This study aimed to evaluate bone repair in critical defects in rat calvaria using a polydioxanone membrane (Plenum® Guide) compared with a commercially available collagen-based membrane (Bio-Gide®). The bone defects were filled with Plenum® Osshp, a synthetic bone graft, hydroxyapatite:ß-tricalcium phosphate, 70:30%, Group PG (Plenum® Guide + Plenum® Osshp), and Group BG (Geistlich Bio-Gide® + Plenum® Osshp). The specimens were submitted to immunohistochemical (RUNX2 and OPN), gene expression (RUNX2, IBSP, and VEGF), histometric, and microtomography analyses after 07, 15, 30, and 60 days postoperative. PG group showed greater immunolabeling area for RUNX2 and OPN, higher gene expression of VEGF (3.15 ± 0.85), and IBSP (24.9 ± 0.59). However, there was no statistical difference between groups in the histometric analysis regarding the percentage of connective tissue PG (0.83 ± 0.45), BG (0.70 ± 0.34), neoformed bone PG (0.60 ± 0.4), BG (0.65 ± 0.51), and remaining biomaterial PG (0.84 ± 0.31), BG (0.91 ± 0.33). In addition, there was no statistical difference between groups by micro-CT analysis. The absorbable-synthetic membrane, Plenum® Guide, is an effective membrane for guided bone regeneration.

Seroprevalence of Toxoplasmosis in Puerperal Women Treated at a Tertiary Referral Hospital / Soroprevalência de toxoplasmose em puérperas atendidas em um hospital terciário de referência

Abstract Objective To evaluate the seroprevalence of toxoplasmosis among puerperal women cared for at a tertiary university hospital and the level of understanding of these puerperal women about toxoplasmosis, vertical transmission, and its prophylaxis. Methods For this cross-sectional study, we evaluated 225 patients using presential interviews, prenatal documentation, and electronic medical records. Data were stored using Research Electronic Data Capture (REDCap) software. Prevalence rates were estimated by the presence of reactive IgG antibodies against Toxoplasma gondii. Data analysis was performed using the chi-square test and calculation of the odds ratio (OR). Seroreactivity to T. gondii and exposure variables (age, educational level, and parity) were analyzed using a confidence interval (95%CI) and a significance level of 5% (p < 0.05). Results The seropositivity rate for T. gondii was 40%. There was no association between seroprevalence and age. Primiparity was a protective factor against seropositivity and low education was a risk factor. Conclusion Knowledge of T. gondii infection and its transmission forms was significantly limited, presenting a risk for acute maternal toxoplasmosis and vertical transmission of this protozoan. Increasing the education level regarding the risk of toxoplasmosis during pregnancy could reduce the rates of infection and vertical transmission of this parasite.

Resumo Objetivo Avaliar a soroprevalência de toxoplasmose entre puérperas atendidas em um hospital universitário terciário e o nível de compreensão dessas puérperas sobre toxoplasmose, transmissão vertical e sua profilaxia. Métodos Para esse estudo transversal, foram avaliadas 225 pacientes utilizando entrevistas presenciais, documentação de pré-natal e prontuário eletrônico. Os dados foram armazenados usando o software Research Electronic Data Capture (REDCap). As taxas de prevalência foram estimadas pela presença de anticorpos IgG reativos contra o Toxoplasma gondii. A análise dos dados foi realizada por meio do teste do qui-quadrado e cálculo do odds ratio (OR). A sororreatividade ao T. gondii e as variáveis de exposição (idade, escolaridade e paridade) foram analisadas, utilizando-se intervalo de confiança (IC95%) e nível de significância de 5% (p < 0,05). Resultados A taxa de soropositividade para T. gondii foi de 40%. Não houve associação entre soroprevalência e idade. A primiparidade foi fator de proteção contra a soropositividade e a baixa escolaridade foi fator de risco. Conclusão O conhecimento da infecção por T. gondii e suas formas de transmissão foi significativamente limitado, apresentando risco para toxoplasmose materna aguda e transmissão vertical desse protozoário. Aumentar o nível de escolaridade quanto ao risco de toxoplasmose durante a gravidez pode reduzir as taxas de infecção e transmissão vertical desse parasita.

Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats.

AIM: The aim of this study was to evaluate the effects of cigarette smoke inhalation (CSI) on inflammation, pro-inflammatory mediators and haematological parameters in rats with induced apical periodontitis (AP). METHODOLOGY: Thirty-two 3-month-old male Wistar rats were divided into four experimental groups (n = 8): C-Control; S-rats with CSI; AP-rats with AP; and SAP-rats with CSI + AP. Animals in groups S and SAP inhaled cigarette smoke by remaining inside a smoking chamber for 8 min, three times daily, for 50 days. After 20 days of smoke inhalation, animals in AP and SAP groups had the pulps of the lower right first molar exposed to oral environment for 30 days to induce AP. In these subsequent 30 days, animals in group S and SAP continued with CSI. On Day 50, animals were euthanized and mandibles were histologically processed to assess inflammatory infiltrate, immunohistochemical interleukins (IL-1ß, IL-6 and TNF-α), and blood samples collected for laboratory analysis. The Mann-Whitney test was performed for non-parametric data and the pairwise analyses of Student's t-test for parametric data, with a significance level of p < .050. RESULTS: Inflammatory infiltrate was moderate in AP group and more severe in the SAP (p = .010). The interleukins IL-6, IL-1ß and TNF-α were higher in SAP group (p < .001) when compared to the AP group. A greater number of red blood cells (p = .010), haemoglobin (p = .007) and neutrophils (p = .014) were observed in the SAP group in comparison with the AP group. CONCLUSION: Cigarette smoke inhalation induced a more severe inflammatory infiltrate, with increased levels of pro-inflammatory cytokines and changes in haematological parameters in rats with induced AP. Thus, CSI aggravated AP, exacerbating the inflammatory response.

Triazoles with inhibitory action on P2X7R impaired the acute inflammatory response in vivo and modulated the hemostatic balance in vitro and ex vivo.

OBJECTIVE: The present study aimed to investigate five triazole compounds as P2X7R inhibitors and evaluate their ability to reduce acute inflammation in vivo. MATERIAL: The synthetic compounds were labeled 5e, 8h, 9i, 11, and 12. TREATMENT: We administered 500 ng/kg triazole analogs in vivo, (1-10 µM) in vitro, and 1000 mg/kg for toxicological assays. METHODS: For this, we used in vitro experiments, such as platelet aggregation, in vivo experiments of paw edema and peritonitis in mice, and in silico experiments. RESULTS: The tested substances 5e, 8h, 9i, 11, and 12 produced a significant reduction in paw edema. Molecules 5e, 8h, 9i, 11, and 12 inhibited carrageenan-induced peritonitis. Substances 5e, 8h, 9i, 11, and 12 showed an anticoagulant effect, and 5e at a concentration of 10 µM acted as a procoagulant. All derivatives, except for 11, had pharmacokinetic, physicochemical, and toxicological properties suitable for substances that are candidates for new drugs. In addition, the ADMET risk assessment shows that derivatives 8h, 11, 5e, and 9i have high pharmacological potential. Finally, docking tests indicated that the derivatives have binding energies comparable to the reference antagonist with a competitive inhibition profile. CONCLUSIONS: Together, the results indicate that the molecules tested as antagonist drugs of P2X7R had anti-inflammatory action against the acute inflammatory response.

Relação do etilismo e do nível de atividade física com fatores de risco para doenças cardiovasculares em estudantes universitários / Relationship between alcohol consumption and physical activity level with risk factors for cardiovascular diseases in college students / Relación del consumo de alcohol y el nivel de actividad física con los factores de riesgo de enfermedades cardiovasculares en estudiantes universitarios

O consumo de bebidas alcoólicas, principalmente em jovens, acarreta uma série de efeitos negativos no sistema cardiovascular. Tal uso exacerbado e precoce pode predispor à calcificações coronarianas, aumento na pressão arterial, arritmias cardíacas e insuficiências coronarianas à longo prazo. Em contrapartida, a prática de atividades físicas é uma grande aliada na saúde cardiovascular. Assim, objetiva-se com o presente estudo associar o consumo de bebidas alcoólicas e a prática de atividade física com fatores de risco para doenças cardiovasculares em estudantes universitários. É um estudo observacional transversal. Foram coletados dados de consumo de bebidas alcoólicas (questionário AUDIT), nível de atividade física (IPAQ), índice de massa corporal (IMC), circunferência de cintura (CC), relação cintura quadril (RCQ), pressão arterial sistêmica e histórico de tabagismo. A comparação entre os grupos realizada pelo teste t-Student ou Mann-Whitney. A correlação entre as variáveis pelo Coeficiente de Spearman. Dessa forma, os resultados encontrados foram que os estudantes universitários que consumiam bebida alcóolica e que tiveram risco de dependência (n=40) tiveram maior IMC (Δ=+1,18kg/m2, p=0,049), CC (Δ=+5,77cm, p=0,005) e RCQ (Δ=+0,003, p=0,027). O score da dieta (Δ=+6,39, p=0,056) do grupo ativo foi maior em relação ao sedentário. Houve correlação direta do score do questionário AUDIT com tabagismo (p<0,001), IMC (p=0,010), CC (p=0,005), RCQ (0,045) e atividade física vigorosa (p=0,027) e, desta com tabagismo (p=0,021). Com o estudo, foi perceptível que há alto consumo de bebidas alcoólicas entre os estudantes universitários, mas, baixo o risco de dependência ao álcool. Os estudantes que são etilistas e com risco de dependência tem maiores valores para parâmetros de obesidade incluindo a RCQ que está diretamente relacionada com evento cardiovascular.

The consumption of alcoholic beverages, especially among young people, has a series of negative effects on the cardiovascular system. Such exacerbated and early use may predispose to coronary calcification, increased blood pressure, cardiac arrhythmias, and long-term coronary insufficiency. On the other hand, the practice of physical activities is a great ally in cardiovascular health. Thus, this study aims to associate the consumption of alcoholic beverages and the practice of physical activity with risk factors for cardiovascular diseases in college students. This is a cross-sectional observational study. We collected data on alcohol consumption (AUDIT questionnaire), physical activity level (IPAQ), body mass index (BMI), waist circumference (WC), waist- to-hip ratio (WHR), systemic blood pressure, and smoking history. The comparison between groups was done by the t-Student or Mann-Whitney test. The correlation between variables was done by Spearman's Coefficient. Thus, the results found were that college students who consumed alcoholic beverages and had a risk of addiction (n=40) had higher BMI (Δ= +1.18kg/m2, p=0.049), WC (Δ=+5.77cm, p=0.005) and WHR (Δ=+0.003, p=0.027). The diet score (Δ=+6.39, p=0.056) of the active group was higher compared to the sedentary group. There was direct correlation of AUDIT questionnaire score with smoking (p<0.001), BMI (p=0.010), WC (p=0.005), WHR (0.045) and vigorous physical activity (p=0.027) and, of this with smoking (p=0.021). With the study, it was noticeable that there is high alcohol consumption among college students, but, low risk of alcohol dependence. Students who are alcohol drinkers and at risk of addiction have higher values for obesity parameters including WHR which is directly related to cardiovascular event. KEYWORDS: Alcohol Consumption in College; Physical Exercise; Heart Disease; Risk Factors; Students.

El consumo de bebidas alcohólicas, especialmente entre los jóvenes, tiene una serie de efectos negativos sobre el sistema cardiovascular. Este consumo exacerbado y precoz puede predisponer a la calcificación coronaria, aumento de la presión arterial, arritmias cardiacas e insuficiencia coronaria a largo plazo. Por otro lado, la práctica de actividades físicas es un gran aliado en la salud cardiovascular. Así, este estudio pretende asociar el consumo de bebidas alcohólicas y la práctica de actividad física con factores de riesgo de enfermedades cardiovasculares en estudiantes universitarios. Se trata de un estudio observacional transversal. Se recogieron datos sobre el consumo de alcohol (cuestionario AUDIT), el nivel de actividad física (IPAQ), el índice de masa corporal (IMC), el perímetro de cintura (CC), la relación cintura-cadera (RCC), la presión arterial sistémica y los antecedentes de tabaquismo. La comparación entre los grupos se realizó mediante la prueba t-Student o Mann-Whitney. La correlación entre las variables por el Coeficiente de Spearman. Así, los resultados encontrados fueron que los universitarios que consumían bebidas alcohólicas y tenían riesgo de dependencia (n=40) presentaban mayor IMC (Δ= +1,18kg/m2, p=0,049), CC (Δ=+5,77cm, p=0,005) y RH (Δ=+0,003, p=0,027). La puntuación de la dieta (Δ=+6,39, p=0,056) del grupo activo fue mayor en comparación con el grupo sedentario. Hubo correlación directa de la puntuación del cuestionario AUDIT con el tabaquismo (p<0,001), el IMC (p=0,010), la CC (p=0,005), el RH (0,045) y la actividad física vigorosa (p=0,027) y, de ésta, con el tabaquismo (p=0,021). Con el estudio, se notó que hay alto consumo de bebidas alcohólicas entre los universitarios, pero, bajo riesgo de dependencia del alcohol. Los estudiantes bebedores de alcohol y con riesgo de dependencia presentan valores más elevados en los parámetros de obesidad, incluyendo el WHR que está directamente relacionado con el evento cardiovascular.

Avaliação do alfabetismo em saúde bucal dos responsáveis por crianças e adolescentes atendidos na clínica escola de odontologia Uninassau Recife / Evaluation of literacy in oral health of those responsible for children and adolescents attended at the Uninassau Recife dental school clinic / Evaluación del alfabetismo en la salud bucal de niños y adolescentes en la escuela de odontología clínica Uninassau Recife

Objetivo: Analisar a influência do nível de alfabetismo dos pais e/ou responsáveis na saúde bucal de crianças e adolescentes atendidos na Clínica Escola de Odontologia do Centro Universitário Maurício de Nassau, em Recife. Métodos: Estudo de campo de desenvolvimento transversal e natureza observacional realizado na Clínica Escola de Odontologia do Centro Universitário Maurício de Nassau, no período de janeiro a abril de 2023. A amostra foi composta por 32 pais e/ou responsáveis. Os instrumentos de coleta utilizados foram: ficha específica, Índice de Placa Visível (IPV) modificado e Índice de dentes cariados, perdidos e obturados (CPO-D)/ Índice de dentes cariados, extraídos e obturados (ceo-d), Critério de Classificação Econômica (CCEB) e Rapid Estimate of Adult Literacy in Dentistry ­ Brazil (REALD-B). Resultados: Observou-se uma predominância do acompanhamento materno nas consultas odontológicas de suas crianças/adolescentes. Quanto a procura por atendimento odontológico para seus filhos, a maioria afirmou buscar em caso de prevenção, diferente do resultado para o atendimento próprio, que prevaleceu os casos de tratamento. Ademais, ao associar o resultado obtido pelo CCEB e teste REALD-B, obteve-se que dos 19 participantes classificados como "ruim", ao acertarem entre 0 a 18 palavras, 18 são da Classe "C-D,E", com nível econômico médio-baixo. A inspeção de saúde bucal das crianças/adolescentes foi considerada razoável, com a média de 2 a 3 dentes acometidos, no entanto a faixa etária predominante da amostra variou entre 3 e 10 anos, ratificando o ínicio precoce dos problemas de saúde bucal. Conclusão: Foi observado uma associação entre o nível de alfabetismo em saúde bucal dos pais e/ou responsáveis e as condições clínicas de saúde bucal de suas crianças/adolescentes.

Objective: To analyze the influence of the level of literacy of parents and/or guardians on the oral health of children and adolescents attended at the Clinical School of Dentistry of the Maurício de Nassau University Center, in Recife. Methods: Field study of cross-sectional development and observational nature conducted at the Clinical School of Dentistry of the University Center Maurício de Nassau, in the period from January to April 2023. The sample consisted of 32 parents and/or guardians. The collection instruments used were: specific token, Visible Plate Index (IPV) modified and Index of decayed, lost and filled teeth (CPO-D)/ Index of decayed, extracted and filled teeth (ceo-d), Economic Classification Criterion (CCEB) and Rapid Estimate of Adult Literacy in Dentistry - Brazil (REALD-B). Results: A predominance of maternal follow- up was observed in the dental consultations of their children/adolescents. As for the search for dental care for their children, most said to seek in case of prevention, different from the result for the own care, which prevailed the cases of treatment. Furthermore, by associating the result obtained by the CCEB and the REALD-B test, it was obtained that of the 19 participants classified as "bad", when they hit between 0 to 18 words, 18 are of the Class "C-D,E", with medium-low economic level. Oral health inspection of children/adolescents was considered reasonable, with the average of 2 to 3 affected teeth, however the predominant age range of the sample ranged between 3 and 10 years, ratifying the early onset of oral health problems. Conclusion: An association was observed between the level of oral health literacy of parents and/or guardians and the clinical conditions of oral health of their children/adolescents.

Propósito: analizar la influencia del nivel de alfabetización de padres y/o funcionarios en la salud oral de niños y adolescentes atendidos en la Clínica de Odontología del Centro Universitario Maurício de Nassau de Recife. Métodos: estudio de campo sobre el desarrollo transversal y el carácter observacional realizado en la Facultad de Odontología del Centro Universitario de Mauricio Nassau, en el período comprendido entre enero y abril de 2023. La muestra consistió en 32 padres y/o funcionarios. Los instrumentos de recolección utilizados fueron: token específico, Índice de Tarjeta Viewable Modificado (IAB) y Índice de Dientes Muertos, Perdidos y Obturados (CPO-D)/ Índice de Dientes Recubierto, Extraídos y Obtuados (CCEB), Criterio de Clasificación Económica (CCEB) y Rapid Estimate of Adult Literacy in Dentistry - Brazil (REALD-B). Resultados: Se observó un predominio del acompañamiento materno en las consultas dentales de sus niños/adolescentes. En cuanto a la búsqueda de atención dental para sus hijos, la mayoría declaró que lo buscarían en caso de prevención, diferente del resultado de su propia atención, que prevalecía en los casos de tratamiento. Además, al asociar el resultado obtenido por la CCEB y la prueba REALD-B, se encontró que de los 19 participantes clasificados como "malos", al alcanzar entre 0 y 18 palabras, 18 pertenecen a la clase "C-D,E", con un nivel económico medio- bajo. La inspección de salud oral de los niños/adolescentes se consideró razonable, con un promedio de 2 a 3 pacientes afectados, pero el grupo de edad predominante de la muestra varió de 3 a 10 años, ratificando el inicio temprano de los problemas de salud oral. Conclusión: Se observó una asociación entre el nivel literario en la salud oral de los padres y/o responsables y las condiciones clínicas de salud oral de sus hijos/adolescentes. PALABRAS CLAVE: Alfabetización en Salud; Salud Bucal; Educación en Salud.