ABSTRACT
OBJECTIVE: To evaluate the effect of fluoride consistency and composition to protect enamel and dentin against the dental erosion. MATERIALS AND METHODS: Bovine enamel and dentin specimens were treated with artificial saliva, neutral fluoride gel (NFG), acidulated phosphate fluoride gel (AFG), neutral fluoride foam (NFF), and acidulated phosphate fluoride foam. The samples were subjected to cycling. Micro energy-dispersive X-ray fluorescence spectrometry, surface roughness (Ra), contact angle (CA), and scanning electron microscopy (SEM) were performed. Composition, CA and Ra data were analyzed by ANOVA and multiple comparison test (p < 0.05). RESULTS: The dentin protected had a significantly higher mineral content than in the control. Eroded unprotected enamel had higher Ra values than normal surfaces. Fluoride treatments increased the Ra in dentin samples. AFG increased the CA in enamel. Fluoride foams increased CA in dentin with reduced mineral loss. SEM analysis found a deposited layer on enamel treated with AFG and remnants of deposits on dentin treated with NFG and NFF. CONCLUSION: Regardless of the form of application, fluoride provided protection against erosion, however with different levels. CLINICAL SIGNIFICANCE: Applying the adequate fluoride form is relevant since the formulations have different effects on both enamel and dentin.
Subject(s)
Dental Enamel , Dentin , Fluorides , Microscopy, Electron, Scanning , Spectrometry, X-Ray Emission , Surface Properties , Tooth Erosion , Animals , Tooth Erosion/prevention & control , Cattle , Dental Enamel/drug effects , Dentin/drug effects , Fluorides/pharmacology , Acidulated Phosphate Fluoride/pharmacology , Saliva, Artificial , Fluorides, Topical/pharmacologyABSTRACT
ABSTRACT BACKGROUND: Nutritional status and growth curves can affect cognitive development, increase the risk of infection, and contribute to the development of chronic diseases. Its etiology is related to food, socioeconomic, and maternal conditions. However, to date, no data on these parameters exist in the state of Goiás, Brazil. OBJECTIVE: To compare the nutritional status and growth curves of children and adolescents in the city of Goiânia, Goiás, Brazil. DESIGN AND SETTING: This was a cross-sectional study. A total of 529 individuals were recruited from a primary health center in the municipality. METHODS: To assess nutritional status, the sample was divided into three categories: 3-4, 5-10, and 11-19 years, with z-score classification considering body mass index for age. The classification of growth curves was performed considering the median height values for age, assuming two references: (a) young Brazilian population and (b) one recommended for international use. The independent sample T-test was used to compare anthropometric variables. RESULTS: The results showed that the classification of eutrophics represents a predominant percentage between both sexes (men: 03-04 = 55.4%; 05-10 = 57.6%; 11-19 = 53.5 % and women: 03-04 = 53.5%; 05-10 = 63.9%; 11-19 = 56.9%), and growth curves showed differences in specific periods in both sexes. CONCLUSIONS: It can be concluded that children and adolescents from the city of Goiânia present as predominance the eutrophic nutritional status, followed by the risk of overweight, underweight, obesity, and malnutrition of both sexes.
ABSTRACT
Functionalization of calcium phosphates with biomimetic peptides is a promising strategy to increase cellular response for bone tissue repair. In this work, biphasic calcium phosphate pellets were functionalized with the hydroxyapatite-binding peptide pVTK by dropping a suspension of the peptide on the pellet surface. The bioactivity tests were performed in vitro by using McCoy culture medium. Cytotoxicity tests were also performed to assess cell viability. The material was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy with field emission gun (FEG-SEM). The results showed that functionalization with the biomimetic peptide was most effective in inducing precipitation of bone-like apatite on the pellets surface, when compared to the control groups (two positive control groups and one negative control group). Cytotoxicity tests showed that all samples are biocompatible but the pellets with peptide showed the highest values of cell viability.
Subject(s)
Biomineralization , Calcium Phosphates , Apatites , Biomimetics , Peptides/pharmacologyABSTRACT
BACKGROUND: Nutritional status and growth curves can affect cognitive development, increase the risk of infection, and contribute to the development of chronic diseases. Its etiology is related to food, socioeconomic, and maternal conditions. However, to date, no data on these parameters exist in the state of Goiás, Brazil. OBJECTIVE: To compare the nutritional status and growth curves of children and adolescents in the city of Goiânia, Goiás, Brazil. DESIGN AND SETTING: This was a cross-sectional study. A total of 529 individuals were recruited from a primary health center in the municipality. METHODS: To assess nutritional status, the sample was divided into three categories: 3-4, 5-10, and 11-19 years, with z-score classification considering body mass index for age. The classification of growth curves was performed considering the median height values for age, assuming two references: (a) young Brazilian population and (b) one recommended for international use. The independent sample T-test was used to compare anthropometric variables. RESULTS: The results showed that the classification of eutrophics represents a predominant percentage between both sexes (men: 03-04 = 55.4%; 05-10 = 57.6%; 11-19 = 53.5 % and women: 03-04 = 53.5%; 05-10 = 63.9%; 11-19 = 56.9%), and growth curves showed differences in specific periods in both sexes. CONCLUSIONS: It can be concluded that children and adolescents from the city of Goiânia present as predominance the eutrophic nutritional status, followed by the risk of overweight, underweight, obesity, and malnutrition of both sexes.
Subject(s)
Nutritional Status , Overweight , Male , Humans , Child , Female , Adolescent , Brazil/epidemiology , Cross-Sectional Studies , Body Mass Index , Overweight/epidemiologyABSTRACT
The aim of the present study was to determine concentrations of cadmium, copper, chromium, manganese and iron in fishes in the São Marcos (SMB), São Jose (SJB) and Arraial (AB) Bays. Metal concentrations were determined using inductively coupled plasma optical emission spectrometry. Mean Cd and Cr levels were above the permissible limits set by different international or national guidelines in all three bays, whereas copper levels were well below the maximum acceptable limit. High concentrations of iron were found in all species analyzed, whereas high concentrations of manganese were found, especially in specimens caught in SJB. Spatial analysis indicated significant differences among the elements investigated. Copper was correlated more with SMB and AB as well as the species Macrodon ancylodon (carnivore) and Sciades herzbergii (omnivore). Cadmium and iron were strongly associated with AB and SJB, while manganese was only associated with SJB, mainly in carnivorous and herbivorous species.
Subject(s)
Catfishes , Metals, Heavy , Water Pollutants, Chemical , Animals , Copper/analysis , Cadmium/analysis , Manganese/analysis , Brazil , Water Pollutants, Chemical/analysis , Metals/analysis , Iron/analysis , Fishes , Environmental Monitoring , Metals, Heavy/analysisABSTRACT
The present study evaluated physicochemical and microbiological variables of the water in the estuary of the Perizes River in the state of Maranhão, Brazil, heavy metal levels in the muscle tissue of fish species (Genyatremus luteus, Macrodon ancylodon, Sciades herzbergii and Mugil curema). Data were collected bimonthly between Nov 2017 and May 2018. Physicochemical variables of the water (pH, salinity, conductivity, dissolved oxygen and turbidity). The microbiological analysis of the water was performed using the multiple-tube method. Atomic absorption spectrophotometry was used for the determination of heavy metals in muscle tissue of the fishes. The results revealed minor fluctuations in the environmental variables, with the exception of turbidity. The microbiological quality of the water was associated with acceptable bacterial levels. Regarding heavy metals, acceptable concentrations were found of Al, Cu, Fe, Mn, Zn, Ni and Hg, whereas Cr and Pb were found at concentrations higher than the maximum.
Subject(s)
Catfishes , Metals, Heavy , Water Pollutants, Chemical , Animals , Rivers , Water Quality , Brazil , Estuaries , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , Metals, Heavy/analysis , FishesABSTRACT
Pott's Puffy tumor, also called Pott's edematous tumor (PET), is a subperiosteal abscess of the frontal bone, associated with osteomyelitis of the frontal bone. In this paper, we report the case of a 16-year-old patient who presented with headache associated with progressive forehead swelling and fever. Clinical and imaging exams pointed to the hypothesis of PET associated with brain abscess. Patient was submitted to surgical excision of the abscess and treatment of osteomyelitis, with intraoperative findings corroborating the condition. There was a good clinical-radiological recovery associated with prolonged antibiotic therapy and satisfactory follow-up after hospital. PET, which often results from an underdiagnosed or partially treated frontal sinusitis, is a condition that must be promptly recognized and directed to an adequate therapeutic approach due to the risk of serious complications that it entails.
ABSTRACT
The aim of this study was to compare two different maximal intensity exercise modality training protocols of similar durations on muscle strength, cardiorespiratory fitness, and lower limb composition in recreationally trained men. Twenty-five trained men (28.9 ± 5.6 years) were randomly divided into Cycle ergometer (4 sets of 30 seconds sprints) and Leg press (4 sets of 10-12 repetitions to momentary failure). Both groups trained three times a week for 5 weeks. Before and after the training period, the participants performed a 10-repetition maximum (10RM) test for knee extension, an incremental exercise test on a treadmill for time to exhaustion (TTE) and peak oxygen consumption (VËO2peak) and underwent dual energy X-ray absorptiometry to assess lower limb composition. Knee extension 10RM and TTE increased in both groups with no statistically significant between group (p = 0.614 and p = 0.210). Only cycle ergometer group increased VËO2peak (p = 0,012). For all lower limb composition outcomes, changes were minimal. The results suggest that 5 weeks of effort and duration matched exercise protocols using cycle ergometer training or leg press may produce similar strength adaptations.
Subject(s)
Cardiorespiratory Fitness , Resistance Training , Ergometry , Exercise Test , Humans , Male , Muscle Strength , Muscle, Skeletal/physiologyABSTRACT
Abstract Aim: This study aimed to verify the reproducibility of an endurance test for master swimmers. Methods: Seventeen male swimmers, aged from 30 to 50 years (35.06 ± 5.36), weight (78.71 ± 13.06 kg), height (174.35 ± 0.07 cm), participated in this study. The participants underwent the Progressive Swim Test (PSwT), which corresponds to swimming progressively to exhaustion in a 25-meter pool, guided by the recording of sound signals indicating the swimming rhythm. We verified the following variables: heart rate before the test (BHR) and right after the end of the test (AHR), followed by the rate of perceived exertion (RPE) and a total of laps performed (SN - swimming number). For comparison between the three days of tests, the one-way repeated measures ANOVA with Tukey's Post-Hoc was performed. Relative reproducibility was performed by the r test and the intraclass correlation coefficient (ICC). Absolute reproducibility was determined using the coefficient of variation (CV) and the confidence interval (CI). Results: The results showed high reproducibility in (PSwT) in master swimmers in the analyzed variables of intergroup and intragroup with relative reproducibility of r > 0.60 and ICC > 0.70 and absolute reproducibility with CI 95% (lower −1.10 and upper 0.99) and CV < 10%. Most of the cases were within the limits of agreement of the Bland-Altman tests, all variables were considered a level of significance with (p <0.05). Conclusion: The Progressive Swim Test (PSwT) is a reproducible instrument in the assessment of endurance in master swimmers.
Subject(s)
Humans , Adult , Middle Aged , Swimming/physiology , Endurance Training , Reproducibility of Results , Analysis of Variance , Heart RateABSTRACT
Impacts on urban water resources are invariably caused by the development of urban centers and the occupation of the watershed. The release of untreated effluents and urban runoff are sources of metals in adjacent water bodies. It is therefore important to conduct studies on the occurrence of these trace elements in the environment and the risks posed to human health. The aim of the present study was to determine Cd, Pb, Cu, and Zn concentrations in water bodies located in the Bacanga and Cachorros watersheds and correlate the occurrence of these elements with physicochemical variables of the water (temperature, pH, dissolved oxygen, and electrical conductivity). The variables were determined at the collection site with the aid of a multiparameter kit. Heavy metal concentrations were determined using anodic stripping voltammetry. The results revealed that the streams were not contaminated with the metals analyzed. The only exception was copper in the Limoeiro and Murtura streams, both of which are located in the industrial complex.
Subject(s)
Environmental Monitoring , Metals, Heavy/analysis , Water Pollutants, Chemical/analysis , Brazil , Copper/analysis , Humans , TemperatureABSTRACT
OBJECTIVES: To understand the actions of nurses toward obesity in primary health care units. METHODS: A phenomenological study was carried out with 12 nurses of a city in the state of Minas Gerais. The interviews were organized into categories and analyzed according to the social phenomenology of Alfred Schütz and literature associated with the theme. RESULTS: The following categories emerged, expressing the actions of nurses toward obesity prevention and control: "Guidance on healthy lifestyle habits", "Barriers to the actions of nurses", and "Focusing specifically on obesity". FINAL CONSIDERATIONS: Considering that guidance on healthy lifestyle habits was mostly carried out during individual and collective activities directed to the general population, regardless of their weight conditions, nurses must reorganize their professional practice in primary health care units by implementing specific actions for users with obesity or at risk of developing it.
Subject(s)
Attitude of Health Personnel , Nurses/psychology , Obesity/psychology , Adult , Brazil , Female , Humans , Male , Middle Aged , Primary Health Care/methods , Qualitative ResearchABSTRACT
ABSTRACT Objectives: To understand the actions of nurses toward obesity in primary health care units. Methods: A phenomenological study was carried out with 12 nurses of a city in the state of Minas Gerais. The interviews were organized into categories and analyzed according to the social phenomenology of Alfred Schütz and literature associated with the theme. Results: The following categories emerged, expressing the actions of nurses toward obesity prevention and control: "Guidance on healthy lifestyle habits", "Barriers to the actions of nurses", and "Focusing specifically on obesity". Final considerations: Considering that guidance on healthy lifestyle habits was mostly carried out during individual and collective activities directed to the general population, regardless of their weight conditions, nurses must reorganize their professional practice in primary health care units by implementing specific actions for users with obesity or at risk of developing it.
RESUMEN Objetivos: Comprender la actuación de enfermeros orientada a la obesidad en la Unidad Básica de Salud. Métodos: Estudio fenomenológico, entrevistando a 12 enfermeros de un municipio de Minas Gerais. Los testimonios fueron organizados en categorías y analizados según fenomenología social de Alfred Schütz y literatura relacionada con la temática. Resultados: Surgieron categorías que expresan la actuación de los enfermeros respecto de la prevención y control de la obesidad: "Orientaciones sobre hábitos de vida saludables", "Obstáculos para la actuación del enfermero" y "Especializarse específicamente en obesidad". Consideraciones Finales: Considerando que las orientaciones sobre hábitos de vida saludables fueron realizadas fundamentalmente durante las actividades individuales y colectivas abiertas al público en general, independientemente de la condición ponderal, señálase la necesidad de que el enfermero reorganice sus prácticas profesionales en la Unidad Básica de Salud, con el objeto de implementar acciones específicas para los pacientes con obesidad y/o con riesgo de desarrollarla.
RESUMO Objetivos: Compreender a atuação de enfermeiros voltada para a obesidade na Unidade Básica de Saúde. Métodos: Pesquisa fenomenológica que entrevistou 12 enfermeiros de um município de Minas Gerais. Os depoimentos foram organizados em categorias e analisados segundo a fenomenologia social de Alfred Schütz e literatura relacionada à temática. Resultados: Emergiram categorias que expressam a atuação dos enfermeiros em relação à prevenção e ao controle da obesidade: "Orientações sobre hábitos saudáveis de vida", "Barreiras à atuação do enfermeiro" e "Voltar-se especificamente para a obesidade". Considerações Finais: Considerando que as orientações sobre hábitos de vida saudáveis eram realizadas predominantemente durante as atividades individuais e coletivas voltadas para o público em geral, independentemente da condição ponderal, sinaliza-se a necessidade de o enfermeiro reorganizar suas práticas profissionais na Unidade Básica de Saúde, de modo a implementar ações específicas para os usuários com obesidade e/ou com risco para desenvolvê-la.
ABSTRACT
ABSTRACT Objectives: To understand the actions of nurses toward obesity in primary health care units. Methods: A phenomenological study was carried out with 12 nurses of a city in the state of Minas Gerais. The interviews were organized into categories and analyzed according to the social phenomenology of Alfred Schütz and literature associated with the theme. Results: The following categories emerged, expressing the actions of nurses toward obesity prevention and control: "Guidance on healthy lifestyle habits", "Barriers to the actions of nurses", and "Focusing specifically on obesity". Final considerations: Considering that guidance on healthy lifestyle habits was mostly carried out during individual and collective activities directed to the general population, regardless of their weight conditions, nurses must reorganize their professional practice in primary health care units by implementing specific actions for users with obesity or at risk of developing it.
RESUMEN Objetivos: Comprender la actuación de enfermeros orientada a la obesidad en la Unidad Básica de Salud. Métodos: Estudio fenomenológico, entrevistando a 12 enfermeros de un municipio de Minas Gerais. Los testimonios fueron organizados en categorías y analizados según fenomenología social de Alfred Schütz y literatura relacionada con la temática. Resultados: Surgieron categorías que expresan la actuación de los enfermeros respecto de la prevención y control de la obesidad: "Orientaciones sobre hábitos de vida saludables", "Obstáculos para la actuación del enfermero" y "Especializarse específicamente en obesidad". Consideraciones Finales: Considerando que las orientaciones sobre hábitos de vida saludables fueron realizadas fundamentalmente durante las actividades individuales y colectivas abiertas al público en general, independientemente de la condición ponderal, señálase la necesidad de que el enfermero reorganice sus prácticas profesionales en la Unidad Básica de Salud, con el objeto de implementar acciones específicas para los pacientes con obesidad y/o con riesgo de desarrollarla.
RESUMO Objetivos: Compreender a atuação de enfermeiros voltada para a obesidade na Unidade Básica de Saúde. Métodos: Pesquisa fenomenológica que entrevistou 12 enfermeiros de um município de Minas Gerais. Os depoimentos foram organizados em categorias e analisados segundo a fenomenologia social de Alfred Schütz e literatura relacionada à temática. Resultados: Emergiram categorias que expressam a atuação dos enfermeiros em relação à prevenção e ao controle da obesidade: "Orientações sobre hábitos saudáveis de vida", "Barreiras à atuação do enfermeiro" e "Voltar-se especificamente para a obesidade". Considerações Finais: Considerando que as orientações sobre hábitos de vida saudáveis eram realizadas predominantemente durante as atividades individuais e coletivas voltadas para o público em geral, independentemente da condição ponderal, sinaliza-se a necessidade de o enfermeiro reorganizar suas práticas profissionais na Unidade Básica de Saúde, de modo a implementar ações específicas para os usuários com obesidade e/ou com risco para desenvolvê-la.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Attitude of Health Personnel , Nurses/psychology , Obesity/psychology , Primary Health Care/methods , Brazil , Qualitative ResearchABSTRACT
OBJECTIVE: to understand the experience of nurses in the approach to tobacco users in primary health care services. METHOD: Qualitative, phenomenological research with 15 nurses who were interviewed between January and March 2017. Their speeches were analyzed and classified into categories. RESULTS: the approach by nurses to tobacco users is performed in an individual and unsystematic manner and in operative groups in accordance with the principles of the cognitive-behavioral therapy. This is a complex approach due to issues related to users themselves and to human, material, and structural resources. The expectations of nurses include supporting smoking cessation and expanding the tobacco use prevention work in educational environments. FINAL CONSIDERATIONS: this study points out that nurses need to use the nursing care systematization in coordination with the recommendations of policies aimed at tobacco use control to perform their role in the promotion, control, and reduction of health complications in tobacco users.
Subject(s)
Nurse-Patient Relations , Nursing Process/trends , Primary Health Care/methods , Tobacco Use/psychology , Attitude of Health Personnel , Humans , Primary Health Care/trends , Smoking Cessation/methods , Smoking Cessation/psychology , Tobacco Use/therapyABSTRACT
ABSTRACT Objective: to understand the experience of nurses in the approach to tobacco users in primary health care services. Method: Qualitative, phenomenological research with 15 nurses who were interviewed between January and March 2017. Their speeches were analyzed and classified into categories. Results: the approach by nurses to tobacco users is performed in an individual and unsystematic manner and in operative groups in accordance with the principles of the cognitive-behavioral therapy. This is a complex approach due to issues related to users themselves and to human, material, and structural resources. The expectations of nurses include supporting smoking cessation and expanding the tobacco use prevention work in educational environments. Final considerations: this study points out that nurses need to use the nursing care systematization in coordination with the recommendations of policies aimed at tobacco use control to perform their role in the promotion, control, and reduction of health complications in tobacco users.
RESUMEN Objetivo: Comprender la experiencia del enfermero en el abordaje a los fumadores en atención primaria de salud. Método: Investigación cualitativa y fenomenológica con 15 enfermeros entrevistadas entre enero y marzo de 2017. Testimonios analizados y organizados en categorías. Resultados: El acercamiento de las enfermeras al fumador se realiza de forma individual y asistemática y en grupos operativos siguiendo los principios de la terapia cognitivo-conductual. Este enfoque es complejo debido a cuestiones relacionadas con el fumador y con los recursos humanos, materiales y estructurales. Las expectativas de las enfermeras incluyen apoyar la cesación tabáquica y expandir el trabajo de prevención del tabaquismo en entornos educativos. Consideraciones finales: Se señala la necesidad de que las enfermeras adecuen la sistematización de la atención de enfermería articulada a las recomendaciones de las políticas de control del tabaquismo, cumpliendo su rol en la promoción, control y reducción de los problemas de salud de los fumadores.
RESUMO Objetivo: compreender a experiência do enfermeiro na abordagem aos usuários tabagistas na Atenção Primária à Saúde. Método: pesquisa qualitativa, fenomenológica, com 15 enfermeiros entrevistados entre janeiro e março de 2017. Os depoimentos foram analisados e organizados em categorias. Resultados: a abordagem dos enfermeiros ao usuário tabagista é realizada de modo individual e assistemático e em grupos operativos seguindo os princípios da terapia cognitivo-comportamental. Essa abordagem é complexa por causa de questões relativas ao próprio usuário e aos recursos humanos, materiais e estruturais. As expectativas dos enfermeiros incluem apoiar a cessação do tabagismo e expandir o trabalho de prevenção do uso do tabaco em espaços educativos. Considerações finais: aponta-se a necessidade de o enfermeiro apropriar-se da sistematização da assistência de enfermagem articulada às recomendações das políticas voltadas para o controle do tabagismo, com vistas a cumprir seu papel na promoção, no controle e na diminuição dos agravos à saúde dos usuários tabagistas.
Subject(s)
Humans , Primary Health Care/methods , Tobacco Use/psychology , Nurse-Patient Relations , Nursing Process/trends , Primary Health Care/trends , Attitude of Health Personnel , Smoking Cessation/methods , Smoking Cessation/psychology , Tobacco Use/therapyABSTRACT
OBJECTIVE: To understand the experience of adults and older people with adherence to venous ulcer care. METHOD: A qualitative study based on the concepts of Alfred Schütz's social phenomenology was carried out with 12 adults and older people undergoing venous ulcer treatment in a specialized service in the state of Minas Gerais. The interviews were carried out from October to November 2015, analyzed, categorized, and discussed based on the theoretical-philosophical framework adopted and on thematic literature. RESULTS: The following thematic categories emerged from the interviews: "beliefs and personal and social activities hindering adherence to care"; "being protagonists in the care relationship with venous ulcers", and "motivation for adherence to care". CONCLUSIONS: The intersubjective relationship established between patients with venous ulcers and healthcare professionals emerged as a driving force for adherence to care. Promoting the reciprocity relationship of perspectives between these social actors may promote greater adherence to venous ulcer care.
Subject(s)
Health Knowledge, Attitudes, Practice , Motivation , Patient Compliance , Varicose Ulcer/therapy , Aged , Brazil , Female , Humans , Male , Middle Aged , Nurse-Patient Relations , Patient Compliance/psychology , Patient Participation , Patient Satisfaction , Qualitative Research , Quality of Life , Social Behavior , Varicose Ulcer/psychologyABSTRACT
Innate immunity receptors (Toll-like receptors/TLRs and RIG-like receptors/RLRs) are important for the initial recognition of Zika virus (ZIKV), modulation of protective immune response, and IFN-α and IFN-ß production. Immunological mechanisms involved in protection or pathology during ZIKV infection have not yet been determined. In this study, we evaluated the mRNA expression of innate immune receptors (TLR3, TLR7, TLR8, TLR9, melanoma differentiation-associated protein 5/MDA-5, and retinoic acid inducible gene/RIG-1), its adapter molecules (Myeloid Differentiation Primary Response Gene 88/Myd88, Toll/IL-1 Receptor Domain-Containing Adaptor-Inducing IFN-ß/TRIF), and cytokines (IL-6, IL-12, TNF-α, IFN-α, IFN-ß, and IFN-γ) in the acute phase of patients infected by ZIKV using real-time PCR in peripheral blood. Patients with acute ZIKV infection had high expression of TLR3, IFN-α, IFN-ß, and IFN-γ when compared to healthy controls. In addition, there was a positive correlation between TLR3 expression compared to IFN-α and IFN-ß. Moreover, viral load is positively correlated with TLR8, RIG-1, MDA-5, IFN-α, and IFN-ß. On the other hand, patients infected by ZIKV showed reduced expression of RIG-1, TLR8, Myd88, and TNF-α molecules, which are also involved in antiviral immunity. Similar expressions of TLR7, TLR9, MDA-5, TRIF, IL-6, and IL-12 were observed between the group of patients infected with ZIKV and control subjects. Our results indicate that acute infection (up to 5 days after the onset of symptoms) by ZIKV in patients induces the high mRNA expression of TLR3 correlated to high expression of IFN-γ, IFN-α, and IFN-ß, even though the high viral load is correlated to high expression of TLR8, RIG-1, MDA-5, IFN-α, and IFN-ß in ZIKV patients.
Subject(s)
Immunity, Innate , Immunologic Factors/biosynthesis , Receptors, Immunologic/biosynthesis , Zika Virus Infection/immunology , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Gene Expression Profiling , Humans , Male , Middle Aged , Real-Time Polymerase Chain Reaction , Viral Load , Zika Virus/isolation & purificationABSTRACT
Resumo OBJETIVO Compreender a experiência de pessoas adultas e idosas frente à adesão aos cuidados com a úlcera varicosa. MÉTODO Pesquisa qualitativa fundamentada em pressupostos da Fenomenologia Social de Alfred Schütz, com 12 pessoas adultas e idosas, em tratamento de úlcera varicosa em Serviço Especializado, em Minas Gerais. Os depoimentos foram obtidos de outubro a novembro de 2015 e analisados, categorizados e discutidos a partir do referencial teórico-filosófico adotado e literatura temática. RESULTADOS Dos depoimentos emergiram as categorias temáticas: "Crenças, atividades pessoais e sociais dificultando a adesão ao cuidado"; "Ser protagonista na relação de cuidado com a úlcera varicosa" e "Motivação para aderir ao cuidado". CONCLUSÕES A relação intersubjetiva estabelecida entre as pessoas com úlcera varicosa e os profissionais de saúde emergiu como força potencializadora da adesão ao cuidado. Valorizar a relação de reciprocidade de perspectivas entre estes atores sociais pode promover maior adesão ao cuidado com a úlcera varicosa.
Resumen OBJETIVO Comprender la experiencia de adultos y ancianos en el cuidado de la úlcera varicosa. MÉTODO Investigación cualitativa fundamentada en supuestos de la Fenomenología Social de Alfred Schütz, con 12 personas adultas / ancianas, en tratamiento de la úlcera varicosa en Servicio Especializado, en Minas Gerais. Los testimonios fueron obtenidos de octubre a noviembre de 2015, siendo analizados, categorizados y discutidos a partir del referencial teórico-filosófico y literatura temática. RESULTADOS De los testimonios surgieron las categorías: "Creencias, actividades personales y sociales dificultando la adhesión al cuidado"; "Ser protagonista en la relación de cuidado con la úlcera varicosa" y "Motivación para adherirse al cuidado". CONCLUSIONES La relación intersubjetiva establecida entre las personas con úlcera varicosa y los profesionales de salud emergió como fuerza potencializadora de la adhesión al cuidado. Valorar la relación de reciprocidad de perspectivas entre estos actores sociales puede promover mayor adhesión al cuidado con la úlcera varicosa.
Abstract OBJECTIVE To understand the experience of adults and older people with adherence to venous ulcer care. METHOD A qualitative study based on the concepts of Alfred Schütz's social phenomenology was carried out with 12 adults and older people undergoing venous ulcer treatment in a specialized service in the state of Minas Gerais. The interviews were carried out from October to November 2015, analyzed, categorized, and discussed based on the theoretical-philosophical framework adopted and on thematic literature. RESULTS The following thematic categories emerged from the interviews: "beliefs and personal and social activities hindering adherence to care"; "being protagonists in the care relationship with venous ulcers", and "motivation for adherence to care". CONCLUSIONS The intersubjective relationship established between patients with venous ulcers and healthcare professionals emerged as a driving force for adherence to care. Promoting the reciprocity relationship of perspectives between these social actors may promote greater adherence to venous ulcer care.
Subject(s)
Humans , Male , Female , Aged , Varicose Ulcer/therapy , Health Knowledge, Attitudes, Practice , Motivation , Patient Participation , Quality of Life , Social Behavior , Varicose Ulcer/psychology , Brazil , Patient Compliance/psychology , Patient Satisfaction , Qualitative Research , Middle Aged , Nurse-Patient RelationsABSTRACT
The quality of aquatic environments all around the world is being altered by different human activities that represent direct threat to the ecological system and the aquatic biota. This study aimed to evaluate the occurrence of histological and genotoxic alterations in Prochilodus lacustris as indicators of anthropic impacts in a lacustrine environment in northeast Brazil. The histological alterations were evaluated using the histological alteration index, and the genotoxic alterations were detected using the micronuclei test, at three sampling stations (S1, S2 and S3). The gills presented lesions with three stages of severity, with mild lesions more frequent in the specimens collected at station S1. Mild hepatic tissue lesions were the most frequent type in both areas. Micronucleus analysis showed that station S3 was the most affected. The biological responses observed indicated that the fish are under influence of environmental changes. It is important to highlight that the organisms collected at station S3 had a more compromised health status.
Subject(s)
Characiformes/metabolism , Ecosystem , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , Animals , Biomarkers/metabolism , Brazil , Characiformes/genetics , DNA Damage , Gills/chemistry , Humans , Micronucleus TestsABSTRACT
Objetivou-se com este estudo, compreender os motivos da não realização da mamografia por mulheres idosas. Pesquisa qualitativa realizada com 13 idosas entre 60 e 69 anos, que nunca realizaram a mamografia. A coleta de dados foi realizada por meio de quatro grupos focais. Os resultados foram organizados em categorias temáticas e discutidos à luz da literatura. As idosas revelaram que entre os motivos para não realizar a mamografia encontra-se a dificuldade de acesso aos serviços de saúde, as crenças relacionadas ao cuidado com as mamas, o constrangimento e o medo do desconforto durante a realização do exame. Considerando-se as barreiras institucionais e pessoais, que limitam a realização da mamografia, ressalta-se a necessidade de ações profissionais que possam aumentar a utilização desse exame para o rastreamento do câncer de mama.
This study aimed to understand the reasons for not doing a mammography among older women. Qualitative research was conducted with 13 elderly women between 60 and 69 years of age who had never done a mammography. Data collection was carried out through four focus groups. The results were organized into thematic categories and discussed considering the literature. These women revealed that among the reasons for not doing a mammography there is the difficulty in access to health services, beliefs related to the care of the breasts, and the embarrassment and fear of discomfort during the exam. Considering the institutional and personal barriers that keep women from undergoing mammography, the need is emphasized for professional actions that can increase the use of mammography to screen for breast cancer.