ABSTRACT
The inlet of wastewater treatment plants (WWTPs) contains pathogenic microorganisms which during aeration and by mechanical mixing through wind typically aerosolized microbes into ambient air. Bioaerosol emission and its characterization (bacterial and fungal) was investigated considering low-flow and high-flow inlet of wastewater treatment plant. Generation of bioaerosols was found influenced by prevailing seasons while both during summer and winter, fungal concentration (winter: 1406 ± 517; summer: 1743 ± 271 CFU/m3) was higher compared to bacterial concentration (winter: 1077 ± 460; summer: 1415 ± 588 CFU/m3). Bioaerosols produced from WWTPs were predominately in the size range of 2.1-4.7 µm while fraction of fungal bioaerosols were also in ultra-fine range (0.65 µm). Bioaerosols reaching to the air from WWTPs varied seasonally and was calculated by aerosolization ratio. During summer, aerosolization of the bioaerosols was nearly 6 times higher than winter. To constitute potential health effects from the exposure to these bioaerosols, biological characterization, antibiotics resistance and the health survey of the nearby area were also performed. The biological characterization of the bioaerosols samples were done through metagenomic approach using 16s and ITS metagenomic sequencing. Presence of 167 genus of bacteria and 41 genus of fungi has been found. Out of this, bacillus (73%), curtobacterium (21%), pseudomonas, Exiguo bacterium, Acinetobacter bacillaceae, Enterobacteriaceae and Prevotella were the dominant genus (top 10) of bacteria. In case of fungi, xylariales (49%), Hypocreales (19%), Coperinopsis (9%), Alternaria (8%), Fusarium (6%), Biopolaris, Epicoccum, Pleosporaceae, Cladosporium and Nectriaceae were dominant. Antibiotics like, Azithromycin and cefixime were tested on the most dominant bacillus showed resistance on higher concentration of cefixime and lower concentration of azithromycin. Population-based health survey in WWTP nearby areas (50-150 m periphery) found several types of diseases/symptoms including respiratory problem, skin rash/irritation, change in smell and taste, eye irritation within the resident population and workers.
Subject(s)
Aerosols , Air Microbiology , Wastewater , Wastewater/microbiology , Aerosols/analysis , Bacteria , Fungi , Environmental Monitoring , HumansABSTRACT
We investigate the room temperature structure (global and local), temperature dependent magnetic and transport behaviour of Ni1-xVx(0⩽x⩽0.13) alloys. Our Energy Dispersive Analysis of x-rays results show that the prepared compositions are stoichiometric. With increase in V doping, the compounds exhibit a quantum phase transition aroundxc= 0.12, where the ferromagnetic phase is suppressed. Our results show that all the compounds stabilize in face centred cubic structure at RT and the lattice parameter shows unusual behaviour close toxc. The magnetic and heat capacity studies show signature of Griffiths phase on either side ofxc. From 25 K to the lowest collected temperature, we observe a linear T dependence of resistivity atx = 0.1 and aroundxc, which is separated by a Fermi-liquid region aroundx = 0.106. This suggests that the origin of the transport behaviour is different around the quantum critical point and away from it. Our Ni K-edge x-ray Absorption Spectroscopy results show that there is a significant reduction in the first coordination number around Ni central atom on doping. Further, with doping, there is distortion in the first coordination shell around Ni. This suggests, with V doping, the local structure around Ni is different from the global structure as obtained from the x-ray Diffraction results. Interestingly, with doping, we observe a direct connection between the extent of distortion at RT and the magnetic disorder obtained at 2 K. We believe our results will motivate the scientific community to further study the interplay between the structural disorder and quantum fluctuations with temperature at the local level.
ABSTRACT
4-Chlorophenol (4-CP) is a persistent organic pollutant commonly found in petrochemical effluents. It causes toxic, carcinogenic and mutagenic effects on human beings and aquatic lives. Therefore, an environmentally benign and cost-effective approach is needed against such pollutants. In this direction, the chlorophenol degrading bacterial consortium consisting of Bacillus flexus GS1 IIT (BHU) and Bacillus cereus GS2 IIT (BHU) was isolated from a refinery site. A composite biocarrier namely polypropylene-polyurethane foam (PP-PUF) was developed for bacterial cells immobilization purpose. A lab-scale moving bed biofilm reactor (MBBR) packed with Bacillus sp. immobilized PP-PUF biocarrier was employed to analyse the effect of peptone on biodegradation of 4-CP. The statistical tool, i.e. response surface methodology (RSM), was used to optimize the process variables (4-CP concentration, peptone concentration and hydraulic retention time). The higher values of peptone concentration and hydraulic retention time were found to be favourable for maximum removal of 4-CP. At the optimized process conditions, the maximum removals of 4-CP and chemical oxygen demand (COD) were obtained to be 91.07 and 75.29%, respectively. In addition, three kinetic models, i.e. second-order, Monod and modified Stover-Kincannon models, were employed to investigate the behaviour of MBBR during 4-CP biodegradation. The high regression coefficients obtained by the second-order and modified Stover-Kincannon models showed better accuracy for estimating substrate degradation kinetics. The phytotoxicity study supported that the Vigna radiata seeds germinated in treated wastewater showed higher growth (i.e. radicle and plumule) than the untreated wastewater.
Subject(s)
Chlorophenols , Wastewater , Humans , Waste Disposal, Fluid/methods , Kinetics , Biofilms , Peptones , Bioreactors , Chlorophenols/metabolismABSTRACT
Present investigation describes immobilization efficiency of endoinulinase onto hetero-functionalized halloysite nanoclay using 3-aminopropyltriethoxysilane and glutaraldehyde as crosslinkers. Under optimal conditions (APTES 0.75%, sonication time 2.25 h, glutaraldehyde 0.75%, activation-time 65 min, immobilized endoinulinase load 60 IU and coupling-time 1 h), maximum yield in enzyme activity (70.65%) and immobilization (89.61%) was obtained. Developed immobilized biocatalyst shown maximum activity at 65 °C and pH 5.0 with wide range thermal (50-80 °C) and pH (4.0-9.0) stability. Increase in half-life (28.70-fold) of immobilized endoinulinase was observed as compared to free enzyme. An enhanced Km and reduced Vmax of endoinulinase for inulin was recorded after immobilization. Maximum FOSs production 98.42% was obtained, under optimized conditions (inulin 10%; immobilized endoinulinase load 85 IU; hydrolysis-time 10 h and agitation rate 130 rpm) containing kestose (36.26%), nystose (27.02%), fructofuranosylnystose (9.98%) and FOSs DP 5-9 (25.15%). Developed immobilized biocatalyst exhibited a splendid operational stability for 18 batch cycles.
Subject(s)
Glycoside Hydrolases , Inulin , Clay , Glutaral , Hydrogen-Ion Concentration , OligosaccharidesABSTRACT
Current work describes the enhancement of immobilization efficacy of Aspergillus tritici endoinulinase onto halloysite nanoclay using crosslinker glutaraldehyde. Under statistical optimized immobilization conditions, viz. glutaraldehyde 1.50% (v/v), enzyme coupling-time 2.20 h, glutaraldehyde activation-time 1.00 h and endoinulinase load 50 IU, maximum activity yield (65.77%) and immobilization yield (82.45%) was obtained. An enhancement of 1.15- and 1.23-fold in both enzyme activity yield and immobilization yield of endoinulinase was observed, when compared with APTES-functionalized halloysite nanoclay immobilized endoinulinase. Immobilized biocatalyst showed maximum activity at pH 5.0 and temperature 60 °C with broad pH (4.0-8.5) and temperature (50-75 °C) stability. Further, optimal hydrolytic conditions (inulin concentration 8.0%; endoinulinase load 80 IU; agitation 125 rpm and hydrolysis-time 13 h) supported fructooligosaccharides yield (95.44%) in a batch system. HPTLC studies blueprint confirmed 95.44% fructooligosaccharides containing 35.41% kestose, 26.19% nystose and 9.69% fructofuranosylnystose. The developed immobilized biocatalyst shown good stability of 8 cycles for inulin hydrolysis.
Subject(s)
Glycoside Hydrolases , Inulin , Clay , Enzyme Stability , Enzymes, Immobilized , Glutaral , Glycoside Hydrolases/metabolism , Hydrogen-Ion Concentration , Oligosaccharides , TemperatureABSTRACT
The COVID-19 disease caused by severe acute respiratory syndrome coronavirus -2 (SARS-CoV-2) has posed as a major health concern for people all across the globe. Along with the increasing confirmed patients being readmitted with complaints for fever, cough, cold, the effective monitoring of 'relapse' of the SARS-CoV-2 virus in the previously discharged patients have become the next area of focus. However, availability of limited data on reactivation of SARS-CoV-2 makes the disease prognosis as well as the effective control of re-infection an immense challenge. Prompted by these challenges, we assessed the possibility of re-infection in discharged patients and the risk of the transmission, proficiency of RT-PCR results and approximate period required for the quarantine, and the real challenges for the development of vaccine. In the present review, the published literature on all the possible cases of re-infection from February to July were reported, thereby selected 142 studies from a hub of overall 669 studies after full text screening. The incomplete virus clearance, poor sensitivity of the present diagnostic testing, emergence of mutant strains, insufficient mucus collection from the throat swab etc., are some of the possible causes of re-infection. The new protocols for management of COVID-19 discharged patients should be revised in the guidelines.
ABSTRACT
Biofuels are considered as a pre-eminent alternate to fossil fuels to meet the demand of future energy supply in a sustainable manner. Conventionally, they are produced from lignocellulosic raw materials. Saccharification of lignocellulosic raw materials for bioethanol production is a cumbersome process as compared to inulin-rich feedstocks. Various inulin-rich feedstocks, viz. jerusalem artichoke, chicory, dahlia, asparagus sp., etc. has also been exploited for the production of biofuels, viz. bioethanol, acetone, butanol, etc. The ubiquitous availability of inulin-rich feedstocks and presence of large amount of inulin makes them a robust substrate for biofuels production. Different strategies, viz. separate hydrolysis and fermentation, simultaneous saccharification and fermentation and consolidated bioprocessing have been explored for the conversion of inulin-rich feedstocks into biofuels. These bioprocess strategies are simple and efficient. The present review elaborates the prospective of inulin-rich feedstocks for biofuels production. Bioprocess strategies exploited for the conversion of inulin-rich feedstocks have also been highlighted.
Subject(s)
Biofuels , Helianthus , Biomass , Fermentation , Helianthus/metabolism , Inulin/metabolism , Prospective StudiesABSTRACT
Airborne transmission is one of the routes for the spread of COVID-19 which is caused by inhalation of smaller droplets1 containing SARS-CoV-2 (i.e., either virus-laden particulate matter: PM and/or droplet nuclei) in an indoor environment. Notably, a significant fraction of the small droplets, along with respiratory droplets, is produced by both symptomatic and asymptomatic individuals during expiratory events such as breathing, sneezing, coughing and speaking. When these small droplets are exposed to the ambient environment, they may interact with PM and may remain suspended in the atmosphere even for several hours. Therefore, it is important to know the fate of these droplets and processes (e.g., physical and chemical) in the atmosphere to better understand airborne transmission. Therefore, we reviewed existing literature focussed on the transmission of SARS-CoV-2 in the spread of COVID-19 and present an environmental perspective on why airborne transmission hasn't been very conclusive so far. In addition, we discuss various environmental factors (e.g., temperature, humidity, etc.) and sampling difficulties, which affect the conclusions of the studies focussed on airborne transmission. One of the reasons for reduced emphasis on airborne transmission could be that the smaller droplets have less number of viruses as compared to larger droplets. Further, smaller droplets can evaporate faster, exposing SARS-CoV-2 within the small droplets to the environment, whose viability may further reduce. For example, these small droplets containing SARS-CoV-2 might also physically combine with or attach to pre-existing PM so that their behaviour and fate may be governed by PM composition. Thus, the measurement of their infectivity and viability is highly uncertain due to a lack of robust sampling system to separately collect virions in the atmosphere. We believe that the present review will help to minimize the gap in our understanding of the current pandemic and develop a robust epidemiological method for mortality assessment.
Subject(s)
COVID-19 , Cough , Exhalation , Humans , Humidity , SARS-CoV-2ABSTRACT
Air pollution due to dust emission is continuously increasing day by day in mining and allied industrial areas. Mining operations contribute a substantial amount of dust emission at the crushing, screening, and bulk material handling in loading areas. The ambient suspended dust particles create a severe nuisance to workers and local dwellers. For effective controlling of positive dust emission, an innovative automated dry fog dust suppression system (DFDSS) has been developed using hybrid nozzles, sensors, actuators, controllers, screw compressors, air receivers, pumps, motors, and water arrangement with filtration facility. The DFDSS was installed in a crushing and screening plant of an iron ore mine in India. Performance study indicted fugitive dust emission concentration values ranged from 354 to 7040 µg m-3, which was reduced to 91-300 µg m-3 after installation of DFDSS. The reduced values were within the permissible limit of 1200 µg m-3 at a distance of 25 ± 2 m in the predominant downwind direction. The installed DFDSS added a meager addition of moisture content of 0.032% in the handling iron ore material, which was below the acceptable limit of 0.1%. The DFDSS precisely regulated fugitive dust emission from various mining activities without affecting the minerals processing performance. Thus, the DFDSS can be implemented effectively in different mining and allied industries where there is a dust emission problem.
Subject(s)
Air Pollutants , Air Pollution , Air Pollutants/analysis , Air Pollution/analysis , Air Pollution/prevention & control , Dust/analysis , Environmental Monitoring , Humans , India , MiningABSTRACT
The objective of present research was to develop an easy, precise and accurate HPTLC densitometry method for quantification of fructooligosaccharides (FOSs) from inulin hydrolysate. The chromatographic separation of FOSs was performed on pre-coated silica gel (60, F254) TLC plates using a mobile phase (butanol:ethanol:water, 60:24:16), and densitometry evaluation of FOSs was performed at A500. Both kestose and nystose were successfully resolved with Rf value of 0.43 and 0.34, respectively. The accuracy, reliability and reproducibility of developed method was assessed by percent relative standard deviation of kestose and nystose for instrument precision (1.43% and 1.50%), repeatability (1.48% and 1.56%), intra-day precision (1.60% and 1.63%), inter-day precision (1.62% and 1.66%), limit of detection (4.58 ng/spot and 4.58 ng/spot), limit of quantification (13.87 ng/spot and 13.89 ng/spot) and recovery (98.81% and 98.69%). Moreover, overlapping spectra of test sample with standard confirms the specificity of developed method, which was validated as per ICH guidelines.
Subject(s)
Densitometry , Inulin/chemistry , Oligosaccharides/analysisABSTRACT
Size-segregated airborne fine (PM2.1) and coarse (PM>2.1) particulates were measured in an urban environment over central Indo-Gangetic plain in between 2015 and 2018 to get insights into its nature, chemistry and sources. Mean (±1σ) concentration of PM2.1 was 98 (±76) µgm-3 with a seasonal high during winter (DJF, 162 ± 71 µgm-3) compared to pre-monsoon specific high in PM>2.1 (MAMJ, 177 ± 84 µgm-3) with an annual mean of 170 (±69) µgm-3. PM2.1 was secondary in nature with abundant secondary inorganic aerosols (20% of particulate mass) and water-soluble organic carbon (19%) against metal enriched (25%) PM>2.1, having robust signature of resuspensions from Earth's crust and road dust. Ammonium-based neutralization of particulate acidity was essentially in PM2.1 with an indication of predominant H2SO4 neutralization in bisulfate form compared to Ca2+ and Mg2+-based neutralization in PM>2.1. Molecular distribution of n-alkanes homologues (C17-C35) showed Cmax at C23 (PM2.1) and C18 (PM>2.1) with weak dominance of odd-numbered n-alkanes. Carbon preference index of n-alkanes was close to unity (PM2.1: 1.4 ± 0.3; PM>2.1: 1.3 ± 0.4). Fatty acids (C12-C26) were characterized with predominance of even carbon with Cmax at n-hexadecanoic acid (C16:0). Low to high molecular weight fatty acid ratio ranged from 2.0 (PM>2.1) to 5.6 (PM2.1) with vital signature of anthropogenic emissions. Levoglucosan was abundant in PM2.1 (758 ± 481 ngm-3) with a high ratio (11.6) against galactosan, emphasizing robust contribution from burning of hardwood and agricultural residues. Receptor model resolves secondary aerosols and biomass burning emissions (45%) as the most influential sources of PM2.1 whereas, crustal (29%) and secondary aerosols (29%) were found responsible for PM>2.1; with significant variations among the seasons.
Subject(s)
Air Pollutants , Particulate Matter , Aerosols/analysis , Air Pollutants/analysis , Carbon/analysis , Dust/analysis , Environmental Monitoring , Particle Size , Particulate Matter/analysis , Seasons , Vehicle Emissions/analysisABSTRACT
Here we have investigated the role of electron phonon coupling on the Raman spectrum of narrow bandgap semiconductors APd3O4 (A = Ca, Sr) and hole-doped system Sr0.85Li0.15Pd3O4. Four Raman active phonons are observed at room temperature for all three compounds as predicted by factor group analysis. The lowest energy phonon (â¼190/202 cm-1) associated with Pd vibrations is observed to exhibit an asymmetric Fano-like lineshape in all the three compounds, indicating the presence of an interaction between the phonon and the electronic continuum. The origin of the electronic continuum states and electron-phonon coupling are discussed based on our laser power- and temperature-dependent Raman results. We have observed an enhanced strength of electron-phonon coupling in Sr0.85Li0.15Pd3O4 at low temperatures which can be attributed to the metallicity in this doped compound.
ABSTRACT
Statistical optimization of hydrolytic conditions for the production of fructooligosaccharides (FOSs) from pure inulin using Aspergillus tritici endoinulinase was carried out in a batch system. FOSs yield 99.19% was obtained under the optimized hydrolytic conditions i.e. inulin concentration (7.3%), enzyme load (65 IU), hydrolysis time (13 h) and agitation (100 rpm). The closeness of value of co-efficient of determination (R2) to 1, good agreement between model's predicted and experimental values, low percentage error (<5%), high adequate precision (>4%) and F value (11,634.32), and low Lack of fit (0.60) of the designed model authenticates its fitness. High substrate concentration, low enzyme load and short hydrolysis span justifies efficiency of developed process for the preparation of FOSs from inulin using fungal endoinulinase. TLC chromatographic and densitometry studies confirmed the synthesis of short-chain length FOSs. FOSs preparation contained 33.85% GF2 (ketose), 24.50% GF3 (nystose), 7.26% GF4 (fructofuranosylnystose) and 33.58% FOSs of DP5-9.
Subject(s)
Biocatalysis , Fungal Proteins/metabolism , Glycoside Hydrolases/metabolism , Inulin/chemistry , Oligosaccharides/chemistry , Aspergillus/enzymology , Fungal Proteins/chemistry , Glycoside Hydrolases/chemistry , Hydrolysis , PolymerizationABSTRACT
Pseudotumour of the lung is a rare chest x-ray finding among patients who present with fluid overload. It is caused by loculated pleural effusion in the lung fissures. Unfortunately, the occurrence of pseudotumour can be misleading and sometimes can lead to unnecessary investigation and emotional stress to the patient. We present here a case of a 61-year-old gentleman with a known history of hypertension, diabetes mellitus and dyslipidemia who presented at University Malaya Medical Centre with symptoms of fluid overload and a right middle lobe mass on chest x-ray. The right middle lobe mass disappeared entirely after being treated with aggressive diuretic therapy. A diagnosis of pseudotumour was made and described in this case report.
Subject(s)
Pleural Effusion , Diuretics/therapeutic use , Humans , Lung , Malaysia , Male , Middle Aged , Pleural Effusion/diagnostic imaging , Pleural Effusion/drug therapy , RadiographyABSTRACT
Owing to its exceptional properties at high temperature, graphite is used in several applications such as structural material and fuel block in high temperature nuclear reactors. Air ingress is one of the serious safety concerns in these reactors. Oxidation of graphite leading to increased porosity affects its mechanical strength and may lead to core collapse resulting in a severe accident. During such a scenario, generation of graphite particles could be the main hazard. Once generated, these particles often in fine and ultrafine sizes, may carry radioactivity to large distances and/or for long times. These particles owing to their higher surface to volume ratio possess an additional inhalation hazard. Ultrafine particles have the potential to enter into respiratory tract and cause damage to body organs. Coating of graphite components is preferred to reduce the oxidation induced damages at high temperatures. In the present work, effect of alumina (Al2O3) coating on the emission characteristics of particles from graphite under high temperature conditions has been investigated. Bare and Al2O3 coated graphite specimens were heated within a closed chamber at varying temperatures during these experiments. Temporal evolution of concentrations of gases (CO and CO2) and particles were measured. The results reveal that Al2O3 coating on the graphite delayed the oxidation behavior and the structure of graphite remained largely intact at high temperatures. A significant reduction in aerosol formation and CO emission was also noticed for the coated specimens.
ABSTRACT
Three-step purification technique (isopropanol precipitation, ion-exchange and size-exclusion chromatography) was used for the purification of an endoinulinase from the culture broth of Aspergillus tritici BGPUP6. The molecular mass of purified endoinulinase was found to be 53.45 kDa and 53.70 kDa by denatured protein gel (SDS-PAGE) and size-exclusion (Sephadex G-100) chromatographic analysis, respectively. Higher Km (0.90 mM), Vmax (19.60 mM/min·mg), Kcat (1.3 × 10-3/min) and Vmax/Km ratio (21.77/min·mg) of purified endoinulinase for inulin than stachyose depicts its higher affinity towards inulin. Purified enzyme was found stable in a pH range 4.0-7.0 with an optimal pH 5.5. The optimal temperature of purified biocatalyst was 55 °C with thermostability in the range of 50-70 °C. D-value and Z-value for endoinulinase at 55 °C was found to be 100.08 h and 11.62 °C, respectively. Thermodynamics inactivation parameters (ΔG, ΔH and ΔS) of endoinulinase shows its wide range thermal stability. Endoinulinase activity was enhanced by CaCl2 and MnSO4, while CuSO4, CoCl2, AgNO3, CdCl2, NiCl2, ZnSO4, BaCl2, HgCl2 and EDTA inhibited the activity of enzyme. Purified endoinulinase was successfully used for the production of fructooligosaccharides from inulin.
Subject(s)
Fungi/enzymology , Glycoside Hydrolases/chemistry , Glycoside Hydrolases/isolation & purification , Inulin/chemistry , Oligosaccharides/chemical synthesis , Thermodynamics , Chemical Fractionation/methods , Chemical Phenomena , Enzyme Activation , Enzyme Stability , Hydrogen-Ion Concentration , Hydrolysis , Ions , Kinetics , MetalsABSTRACT
Inulinases are inulin catalyzing enzymes which belongs to glycoside hydrolases (GH) family 32. Bacteria, fungi and yeasts are the potential sources of inulinases. In the present biotechnological era, inulinases are gaining considerable attention, due to their wide range of applications which includes the production of high fructose syrup, fructooligosaccharides and many other important metabolites like bioethanol, organic acids, single cell oil, 2,3-butanediol, single cell proteins, etc. These applications of inulinases have attracted the researchers world-wide to understand the inulin-inulinase interactions for polyfructan hydrolysis. To understand these interactions, the information on structural organization of inulinases is very important which is scarce in literature. The current review highlights the structural and functional properties of inulinases, and difference in their structural organization. The biotechnological potential of inulinases for the production of different bio-products from inulin/inulin-rich raw materials using different bioprocessing strategies has also been elaborated.
Subject(s)
Biotechnology/methods , Glycoside Hydrolases , Bacterial Proteins/chemistry , Bacterial Proteins/pharmacology , Biofuels , Crystallography, X-Ray , Fungal Proteins/chemistry , Fungal Proteins/pharmacology , Glycoside Hydrolases/chemistry , Glycoside Hydrolases/pharmacology , High Fructose Corn Syrup/metabolism , Industrial Microbiology , Inulin/metabolism , Models, Molecular , Oligosaccharides/metabolism , Organic Chemicals/metabolism , Protein Conformation , Structure-Activity RelationshipABSTRACT
The impact of COVID-19 is changing with country wise and depend on universal immunization policies. COVID-19 badly affects countries that did not have universal immunization policies or having them only for the selective population of countries (highly prominent population) like Italy, USA, UK, Netherland, etc. Universal immunization of BCG can provide great protection against the COVID-19 infection because the BCG vaccine gives broad protection against respiratory infections. BCG vaccine induces expressions of the gene that are involved in the antiviral innate immune response against viral infections with long-term maintenance of BCG vaccine-induced cellular immunity. COVID-19 cases are reported very much less in the countries with universal BCG vaccination policies such as India, Afghanistan, Nepal, Bhutan, Bangladesh, Israel, Japan, etc. as compared to without BCG implemented countries such as the USA, Italy, Spain, Canada, UK, etc. BCG vaccine provides protection for 50-60 years of immunization, so the elderly population needs to be revaccinated with BCG. Several countries started clinical trials of the BCG vaccine for health care workers and elderly people. BCG can be uses as a prophylactic treatment until the availability of the COVID-19 vaccine.
Subject(s)
BCG Vaccine/administration & dosage , Coronavirus Infections/epidemiology , Coronavirus Infections/prevention & control , Pandemics/prevention & control , Pneumonia, Viral/epidemiology , Pneumonia, Viral/prevention & control , Adaptive Immunity , BCG Vaccine/immunology , Betacoronavirus/immunology , COVID-19 , COVID-19 Vaccines , Clinical Trials as Topic , Coronavirus Infections/immunology , Humans , Immunization, Secondary , Mass Vaccination , Pneumonia, Viral/immunology , SARS-CoV-2 , Viral VaccinesABSTRACT
Sources of airborne particulates (PM10) were investigated in two contrasting sites over central Indo-Gangetic Plain (IGP), one representing a rural background (Mirzapur) and another as an urban pollution hotspot (Varanasi). Very high PM10 concentration was noted both in Varanasi (178 ± 105 µgm-3; N:435) and Mirzapur (131 ± 56 µgm-3; N:169) with 72% and 62% of monitoring days exceeded the national air quality standard, respectively. Particulate-bound elements contribute significant proportion of PM10 mass (15%-18%), with highest contribution from Ca (7%-10%) and Fe (2%-3%). Besides, presence of Zn (1%-3%), K (1%-2%) and Na (1%-2%) was also noted. Water-soluble ionic species contributed 15%-19% of particulate mass, primarily by the secondary inorganic aerosols (SIA). Among the SIA, sulphate (5%-7%) and nitrate (4%) were prominent, contributing 59%-62% of the total ionic load, especially in winter. Particulate-bound metallic species and ions were selectively used as signatory molecules and source apportionment of PM10 was done by multivariate factor analysis. UNMIX was able to extract particulate sources in both the locations and crustal resuspensions (dust/-soil) were identified as the dominant source contributing 57%-63% of PM10 mass. Secondary aerosols were the second important source (17%-23%), followed by emissions from biomass/-refuse burning (10-19%). Transport of airborne particulates from upper IGP by prevailing westerly were identified as the important contributor of particulates, especially during high particulate loading days. Health risks associated to particulate-bound toxic metal exposure were also assessed. Non-carcinogenic health risk was within the permissible limit while there is possibility of elevated risk for PM10-bound Cr and Cd, if adequate control measures are not in place.
Subject(s)
Air Pollutants/analysis , Environmental Monitoring/methods , Particulate Matter/analysis , Aerosols/analysis , Air Pollution/analysis , Biomass , Coal/analysis , Dust/analysis , Ions/analysis , Metals/analysis , Risk Assessment , Seasons , Vehicle Emissions/analysisABSTRACT
The performance of a moving bed biofilm reactor (MBBR) with bio-carriers made of polypropylene-polyurethane foam (PP-PUF) was evaluated for the collective removal of phenol and ammonia. Three independent variables, including pH (5.0-8.0), retention time (2.0-12.0 h), and airflow rate (0.8-3.5 L/min) were optimized using central composite design (CCD) of response surface methodology (RSM). The maximum removal of phenol and ammonia was obtained to be 92.6, and 91.8%, respectively, in addition to the removal of 72.3% in the chemical oxygen demand (COD) level at optimum conditions. First-order and second-order kinetic models were analyzed to evaluate the pollutants removal kinetics in a MBBR. Finally, a second-order model was found to be appropriate for predicting reaction kinetics. The values of second-order rate constants were obtained to be 2.35, 0.25, and 1.85 L2/gVSS gCOD h for phenol, COD, and ammonia removal, respectively.