Rational design of Boerhavia diffusa derived CoFe2O4-Carbon dots@Boehmite platform for photocatalysis and ultra trace monitoring of hazardous pesticide and UO22+ ions.

In view of exploiting natural resources for designing of effectual materials in favor of detection and obliteration of water pollutants, a fluorescent nanomaterial (CDBHCF) based on biomass derived carbon dots (CDs) was constructed. The CDs and cobalt ferrite (CF) particles were anchored on boehmite (BH) which served as a support material for CDs. The CDBHCF nanocomposite was prepared via facile hydrothermal treatment for selective recognition of Methyl parathion (MP) pesticide and uranyl ions (UO22+). The corresponding structural, morphological and opto-electronic properties of the nanomaterials have been investigated by different physicochemical techniques. The fluorescent CDBHCF probe was employed to detect extremely low concentration of MP and UO22+ with detection limit of 22.4 nM and 4.4 nM, respectively. Ultimately, the proposed sensing platform was validated through real sample analysis. Besides, CDBHCF nanocomposite was utilized for photocatalytic abolition of Tetracycline (TC) in water samples. Initially, the impact of various operational parameters on the degradation efficiency, including catalyst dosage and initial pH were thoroughly examined. Under optimized conditions, the fabricated CDBHCF nanocomposite demonstrated excellent results for photocatalytic degradation of TC (92 % degradation in 120 min) under visible light illumination. Thus, the proposed strategy delivered an innovative insight for dual purpose of CDBHCF nanocomposite: as a fluorescent probe for real time monitoring and as a photocatalyst for removal of pollutants via simple photocatalytic degradation.

Genome and life-history evolution link bird diversification to the end-Cretaceous mass extinction.

Complex patterns of genome evolution associated with the end-Cretaceous [Cretaceous-Paleogene (K-Pg)] mass extinction limit our understanding of the early evolutionary history of modern birds. Here, we analyzed patterns of avian molecular evolution and identified distinct macroevolutionary regimes across exons, introns, untranslated regions, and mitochondrial genomes. Bird clades originating near the K-Pg boundary exhibited numerous shifts in the mode of molecular evolution, suggesting a burst of genomic heterogeneity at this point in Earth's history. These inferred shifts in substitution patterns were closely related to evolutionary shifts in developmental mode, adult body mass, and patterns of metabolic scaling. Our results suggest that the end-Cretaceous mass extinction triggered integrated patterns of evolution across avian genomes, physiology, and life history near the dawn of the modern bird radiation.

pipesnake: generalized software for the assembly and analysis of phylogenomic datasets from conserved genomic loci.

MOTIVATION: Phylogenetics has moved into the era of genomics, incorporating enormous volumes of data to study questions at both shallow and deep scales. With this increase in information, phylogeneticists need new tools and skills to manipulate and analyze these data. To facilitate these tasks and encourage reproducibility, the community is increasingly moving toward automated workflows. RESULTS: Here we present pipesnake, a phylogenomics pipeline written in Nextflow for the processing, assembly, and phylogenetic estimation of genomic data from short-read sequences. pipesnake is an easy to use and efficient software package designed for this next era in phylogenetics. AVAILABILITY AND IMPLEMENTATION: pipesnake is publicly available on GitHub at https://github.com/AusARG/pipesnake and accompanied by documentation and a wiki/tutorial.

The macroevolutionary singularity of snakes.

Snakes and lizards (Squamata) represent a third of terrestrial vertebrates and exhibit spectacular innovations in locomotion, feeding, and sensory processing. However, the evolutionary drivers of this radiation remain poorly known. We infer potential causes and ultimate consequences of squamate macroevolution by combining individual-based natural history observations (>60,000 animals) with a comprehensive time-calibrated phylogeny that we anchored with genomic data (5400 loci) from 1018 species. Due to shifts in the dynamics of speciation and phenotypic evolution, snakes have transformed the trophic structure of animal communities through the recurrent origin and diversification of specialized predatory strategies. Squamate biodiversity reflects a legacy of singular events that occurred during the early history of snakes and reveals the impact of historical contingency on vertebrate biodiversity.

Repeated patterns of reptile diversification in Western North America supported by the Northern Alligator Lizard (Elgaria coerulea).

Understanding the processes that shape genetic diversity by either promoting or preventing population divergence can help identify geographic areas that either facilitate or limit gene flow. Furthermore, broadly distributed species allow us to understand how biogeographic and ecogeographic transitions affect gene flow. We investigated these processes using genomic data in the Northern Alligator Lizard (Elgaria coerulea), which is widely distributed in Western North America across diverse ecoregions (California Floristic Province and Pacific Northwest) and mountain ranges (Sierra Nevada, Coastal Ranges, and Cascades). We collected single-nucleotide polymorphism data from 120 samples of E. coerulea. Biogeographic analyses of squamate reptiles with similar distributions have identified several shared diversification patterns that provide testable predictions for E. coerulea, including deep genetic divisions in the Sierra Nevada, demographic stability of southern populations, and recent post-Pleistocene expansion into the Pacific Northwest. We use genomic data to test these predictions by estimating the structure, connectivity, and phylogenetic history of populations. At least 10 distinct populations are supported, with mixed-ancestry individuals situated at most population boundaries. A species tree analysis provides strong support for the early divergence of populations in the Sierra Nevada Mountains and recent diversification into the Pacific Northwest. Admixture and migration analyses detect gene flow among populations in the Lower Cascades and Northern California, and a spatial analysis of gene flow identified significant barriers to gene flow across both the Sierra Nevada and Coast Ranges. The distribution of genetic diversity in E. coerulea is uneven, patchy, and interconnected at population boundaries. The biogeographic patterns seen in E. coerulea are consistent with predictions from co-distributed species.

Ultra-sensitive detection and scavenging of arsenic ions and ciprofloxacin using 3D multipurpose hemicellulose based aerogel: Adsorption mechanism and RSM optimization.

Trace level detection and efficient removal of arsenite ions (As (III)) and ciprofloxacin (CPR) antibiotic was achieved using hemicellulose based ratiometric fluorescent aerogel. Hemicellulose derived from rice straw was oxidised to dialdehyde hemicellulose followed by crosslinking using chitosan via a Schiff base reaction (C = N) yielding a highly porous 3D fluorescent aerogel (CS@DAHCA). Various factors governing adsorption were analyzed by applying response surface methodology (RSM) approach. CS@DAHCA exhibited ultra-trace level monitoring with the limit of detection of 3.529 pM and 55.2 nM for As (III) and CPR, respectively. The CS@DAHCA showed maximum adsorption capacity of 185 µg g-1 and 454 mg g-1 for As (III) and CPR, respectively. Finally, the feasibility of CS@DAHCA was ascertained for real water samples confirming it as promising candidate for remediation of As (III) and CPR.

Notes from the taxonomic disaster zone: Evolutionary drivers of intractable species boundaries in an Australian lizard clade (Scincidae: Ctenotus).

Genomic-scale datasets, sophisticated analytical techniques, and conceptual advances have disproportionately failed to resolve species boundaries in some groups relative to others. To understand the processes that underlie taxonomic intractability, we dissect the speciation history of an Australian lizard clade that arguably represents a "worst-case" scenario for species delimitation within vertebrates: the Ctenotus inornatus species group, a clade beset with decoupled genetic and phenotypic breaks, uncertain geographic ranges, and parallelism in purportedly diagnostic morphological characters. We sampled hundreds of localities to generate a genomic perspective on population divergence, structure, and admixture. Our results revealed rampant paraphyly of nominate taxa in the group, with lineages that are either morphologically cryptic or polytypic. Isolation-by-distance patterns reflect spatially continuous differentiation among certain pairs of putative species, yet genetic and geographic distances are decoupled in other pairs. Comparisons of mitochondrial and nuclear gene trees, tests of nuclear introgression, and historical demographic modelling identified gene flow between divergent candidate species. Levels of admixture are decoupled from phylogenetic relatedness; gene flow is often higher between sympatric species than between parapatric populations of the same species. Such idiosyncratic patterns of introgression contribute to species boundaries that are fuzzy while also varying in fuzziness. Our results suggest that "taxonomic disaster zones" like the C. inornatus species group result from spatial variation in the porosity of species boundaries and the resulting patterns of genetic and phenotypic variation. This study raises questions about the origin and persistence of hybridizing species and highlights the unique insights provided by taxa that have long eluded straightforward taxonomic categorization.

Highly fluorescent composite of boron nitride quantum dots decorated on cellulose nanofibers for detection and removal of Hg(II) ions from waste water.

To address the challenge of heavy-metal ions in wastewater, boron nitride quantum dots (BNQDs) were synthesized in-situ on rice straw derived cellulose nanofibers (CNFs) as substrate. The composite system exhibited strong hydrophilic-hydrophobic interactions, as corroborated by FTIR, integrated the extraordinary fluorescence properties of BNQDs with fibrous-network of CNFs (BNQD@CNFs) yielding a surface of 35.147 m2 g-1 of luminescent fibers. Morphological studies revealed uniform distribution of BNQDs on CNFs due to hydrogen bonding, according high thermal stability with peak degradation occurring at 347.7 °C and quantum yield of 0.45. The nitrogen-rich surface of BNQD@CNFs exhibited strong affinity for Hg(II), quenching the fluorescence intensity due to combined inner-filter effect and photo-induced electron transfer. The limit of detection (LOD) and limit of quantification (LOQ) were 4.889 nM and 11.1 5 nM, respectively. BNQD@CNFs concomitantly exhibited adsorption of Hg(II) owing to strong electrostatic interactions, confirmed by X-ray photon spectroscopy. Presence of polar BN bonds favoured 96 % removal of Hg(II) at 10 mg L-1 with maximum adsorption capacity of 314.5 mg/ g. Parametric studies corresponded to pseudo-second order kinetics and Langmuir isotherm with R2 ≈ 0.99. BNQD@CNFs exhibited recovery rate between 101.3 %-111 % for real water samples and recyclability upto 5 cycles, demonstrating high potential in wastewater remediation.

Appraising the electrocatalytic performance of beta-cyclodextrin embellished supramolecular recognition system for pernicious food colorants.

The current research presents the evaluation of supramolecular proficiency of the designed platform for electrocatalytic determination of pernicious food colorants, amaranth and fast green. The approach involving surface modification of glassy carbon electrode with beta cyclodextrin decorated strontium ferrite reduced graphene oxide nanocomposite (SFrGO-ßCD) to ensure fast and reversible electro-oxidation of hydroxyl groups of the colorant molecules. The synergy between SF and rGO facilitated the sensor with enhanced surface area and conductivity through faradic redox reaction. Tremendous decrease in the obtained values of peak separation potential and impedance as manifested in CV and EIS analysis, enabled by electrostatic interactions between surface functionalities of rGO and ßCD has resulted in the significant augmentation of sensitivity. The value of charge transfer coefficient, number of electrons involved, nature of electron transport process at electrode electrolyte interface during the analysis of electrochemical detection were explored through CV experiments. Food samples analysis (without spiking) utilizing screen printed electrode manifested the sensor as portable device for real time monitoring. Outstanding detection limit (0.022 nM for amaranth and 0.051 nM for fast green), excellent regenerability (Relative standard deviation less than 3%) and apparent recovery rate (above 90%) of the modified electrode presented a colossal potential for the development of sustainable and commercially competitive electrochemical sensor in food sector.

Bioremediation of multifarious pollutants using laccase immobilized on magnetized and carbonyldiimidazole-functionalized cellulose nanofibers.

An easily recyclable biocatalyst (Lac@CDI-MCNFs) was synthesized by immobilizing laccase on rice straw-derived carbonyldiimidazole mediated magnetized cellulose nanofibers (MCNFs). Lac@CDI-MCNFs were utilized for bioremediation of cefixime antibiotic (CT), carbofuran pesticide (CF) and safranin O dye (SO) via oxidation-reduction reactions in wastewater. MCNFs provided enhanced pH, temperature and storage stability to laccase and allowed reusability for up to 25 cycles with mere 20 % decline in efficacy. The Lac@CDI-MCNFs effectively degraded 98.2 % CT and 96.8 % CF into benign metabolites within 20 h and completely degraded SO in just 7 h. Response surface modelling (RSM) was employed based on the Box Behnken Design to evaluate the effect of various parameters i.e. pH, catalyst dosage and the pollutants concentration which was further validated with experimental studies. The degradation products were identified using LCMS, which allowed the degradation pathway of the pollutants to be determined. The degradation of all pollutants followed first- order kinetics with rate constants of 0.1775, 0.0832 and 0.958 h-1 and half-life of 3.9, 5.0 and 0.723 h for CT, CF and SO, respectively. Lac@CDI-MCNFs was demonstrated to be an effective catalyst for the degradation of multifarious pollutants.

Cold kit for Rhenium-188 microspheres based selective intra-arterial therapy (SIRT): Preparation, characterization and feasibility study.

Selective-intra-arterial radionuclide therapy (SIRT) using radiolabeled microspheres are being widely employed for the delivery of therapeutic radioisotope to liver cancers by exploiting the dual blood supply to liver. It delivers the therapeutic radiations to tumor and spares the healthy liver. Several radiolabeled microspheres formulations, labelled with 90Y, are commercially available. However, high-cost leads to unaffordability for several patients. 188Re-based therapy seems affordable due to commercial availability of 188W/188Re generator that have long shelf-life of more than 6 months. To provide affordable solution, the microsphere cold kit with quick and facile methodology for 188Re radiolabeling has been developed. The microsphere cold kit has been characterized for their physicochemical properties. The Quality Control (QC) tests were also performed for clinical application. The feasibility studies were performed to study distribution and retention of 188Re microspheres in tumor. The results demonstrated that the developed cold kit enables facile and quick radiolabeling with 188Re. 188Re microspheres showed good retention in tumor and found suitable for SIRT.

Genetic variability and the ecology of geographic range: A test of the central-marginal hypothesis in Australian scincid lizards.

For many species, both local abundance and regional occupancy are highest near the centre of their geographic distributions. One hypothesis for this pattern is that niche suitability declines with increasing distance from a species geographic centre, such that populations near range margins are characterized by reduced density and increased patchiness. In these smaller edge populations, genetic drift is more powerful, leading to the loss of genetic diversity. This simple verbal model has been formalized as the central-marginal hypothesis, which predicts that core populations should have greater genetic diversity than edge populations. Here, we tested the central-marginal hypothesis using a genomic data set of 25 species-level taxa of Australian scincid lizards in the genera Ctenotus and Lerista. A majority of taxa in our data set showed range-wide patterns of genetic variation consistent with central-marginal hypothesis, and eight of 25 taxa showed significantly greater genetic diversity in the centre of their range. We then explored biological, historical, and methodological factors that might predict which taxa support the central-marginal hypothesis. We found that taxa with the strongest evidence for range expansion were the least likely to follow predictions of the central-marginal hypothesis. The majority of these taxa had range expansions that originated at the range edge, which led to a gradient of decreasing genetic diversity from the range edge to the core, contrary to the central-marginal hypothesis.

2-(Anthracen-9-yl)benzothiazole-modified graphene oxide-nickel ferrite nanocomposite for anodic stripping voltammetric detection of heavy metal ions.

A novel electrochemical sensor, 2-(anthracen-9-yl)benzothiazole (ABT)-modified nickel ferrite reduced graphene oxide (NF@rGO) has been designed for the individual and simultaneous detection of Cd2+, Cu2+, and Hg2+ ions. Herein, NF@rGO nanocomposite, synthesized by a simple hydrothermal methodology, was hooked to ABT under easy and simple stirring conditions. Chelation of active functional groups of ABT with metal ions was augmented with higher adsorption and conductivity provided by NF@rGO. The created synergy resulted in analytical signals via selective oxidation of the ions within a potential ranging from - 1.2 to + 1.2 V vs sat. KCl. The proposed protocol exhibited a wide linear range from 0.05 to 1250 nM with excellent detection limit of 123, 54.1, and 86.6 pM via anodic stripping voltammetry for the simultaneous determination of Cd2+, Cu2+, and Hg2+ ions, respectively. Simple cost-effective synthetic approach, improved sensitivity with high selectivity, noteworthy repeatability (RSD less than 3%), and reproducibility (RSD less than 7%) equipped with successful real time monitoring (apparent recovery more than 90%) bring about a spiffing sensing platform for the detection of hazardous metal ions.

No link between population isolation and speciation rate in squamate reptiles.

Rates of species formation vary widely across the tree of life and contribute to massive disparities in species richness among clades. This variation can emerge from differences in metapopulation-level processes that affect the rates at which lineages diverge, persist, and evolve reproductive barriers and ecological differentiation. For example, populations that evolve reproductive barriers quickly should form new species at faster rates than populations that acquire reproductive barriers more slowly. This expectation implicitly links microevolutionary processes (the evolution of populations) and macroevolutionary patterns (the profound disparity in speciation rate across taxa). Here, leveraging extensive field sampling from the Neotropical Cerrado biome in a biogeographically controlled natural experiment, we test the role of an important microevolutionary process-the propensity for population isolation-as a control on speciation rate in lizards and snakes. By quantifying population genomic structure across a set of codistributed taxa with extensive and phylogenetically independent variation in speciation rate, we show that broad-scale patterns of species formation are decoupled from demographic and genetic processes that promote the formation of population isolates. Population isolation is likely a critical stage of speciation for many taxa, but our results suggest that interspecific variability in the propensity for isolation has little influence on speciation rates. These results suggest that other stages of speciation-including the rate at which reproductive barriers evolve and the extent to which newly formed populations persist-are likely to play a larger role than population isolation in controlling speciation rate variation in squamates.

Genetic and Ecogeographic Controls on Species Cohesion in Australia's Most Diverse Lizard Radiation.

AbstractSpecies vary extensively in geographic range size and climatic niche breadth. If range limits are primarily determined by climatic factors, species with broad climatic tolerances and those that track geographically widespread climates should have large ranges. However, large ranges might increase the probability of population fragmentation and adaptive divergence, potentially decoupling climatic niche breadth and range size. Conversely, ecological generalism in large-ranged species might lead to higher gene flow across climatic transitions, increasing species' cohesion and thus decreasing genetic isolation by distance (IBD). Focusing on Australia's iconic Ctenotus lizard radiation, we ask whether species range size scales with climatic niche breadth and the degree of population isolation. To this end, we infer independently evolving operational taxonomic units (OTUs), their geographic and climatic ranges, and the strength of IBD within OTUs based on genome-wide loci from 722 individuals spanning 75 taxa. Large-ranged OTUs were common and had broader climatic niches than small-ranged OTUs; thus, large ranges do not appear to simply result from passive tracking of widespread climatic zones. OTUs with larger ranges and broader climatic niches showed relatively weaker IBD, suggesting that large-ranged species might possess intrinsic attributes that facilitate genetic cohesion across large distances and varied climates. By influencing population divergence and persistence, traits that affect species cohesion may play a central role in large-scale patterns of diversification and species richness.

Sensing and annihilation of ultra-trace level arsenic (III) using fluoranthene decorated fluorescent nanofibrous cellulose probe.

Besides presence of heavy metals, especially arsenic in water bodies, northern India is striving to obliterate crop residue, which is otherwise burnt to make the fields ready for subsequent crop, causing acute air pollution. Through this study, an effort has been made to utilize wheat-straw cellulose to develop inexpensive and efficacious sensing cum annihilation system for deleterious arsenite ions As(III) in water by grafting a novel fluorophore, 3-bromofluoranthene on cellulose (BF@CFs). BF@CFs were characterized for structural, morphological and thermal properties using FTIR, XRD, TGA, FESEM, EDS and TEM, which confirmed the successful insertion of fluoranthene molecule on cellulose while preserving its crystalline nanofibrous structure. Fluorescent studies indicated strong affinity of BF@CFs towards arsenite ions exhibiting "turn on" fluorescence response attributed to inhibition of photo induced electron transfer (PET) and metal ion chelation with a limit of detection of 2.8 ng L-1, lower than WHO prescribed limit of 10 µg L-1. Besides sensing, the porous fibrous network of BF@CFs exhibited good adsorption of As(III) ions with maximum adsorption of 171.2 µg g-1 at 35 min under optimized conditions. BF@CFs displayed 95.2% removal efficiency with 2 µg L-1 concentration of As (III) ions at room temperature and neutral pH observed by atomic absorption spectrophotometer coupled with hydride generation assembly (HG-AAS) measurements. BF@CFs retained adsorption 97.3% efficiency after five adsorption/ desorption cycles displaying excellent reusability and stability, strengthening its potential as dual functional sensor and adsorbent.

Amelioration of adsorptive efficacy by synergistic assemblage of functionalized graphene oxide with esterified cellulose nanofibers for mitigation of pharmaceutical waste.

An effort has been undertaken for valorization of surplus biomass to synthesize sustainable and commercially competitive nanoadsorbents utilizing green synthetic strategies. This study encompasses a pioneering research on the comparative adsorption analysis of different modified forms of graphene oxide (GO) combined with functionalized cellulose nanofibers (CNF) derived from surplus biomass for elimination of noxious drug species from aqueous environment with a comprehensive study for evaluating the effect of loading percentage of functionalized GO. Characteristic assessments of the prepared nanocomposites were performed using FT-IR studies, powder XRD studies, FESEM analysis, EDS analysis and BET studies. The prepared nanohybrids were evaluated for their adsorptive performance for elimination of ciprofloxacin and ofloxacin and their performance was optimized in terms of adsorbent loading, pH and initial drug concentration. Further, investigation of adsorbent properties and the adsorption process was undertaken by studying different kinetic and isotherm models of adsorption. The adsorption potential of functionalized CNF was substantially ameliorated through its facile assemblage with functionalized GO. The experimental outcomes revealed that 20 wt% loading of carboxylated graphene oxide within the perforated surface of esterified cellulose nanofibres exhibited best adsorption performance with maximum removal capacity of 45.04 mg g-1 and 85.30 mg g-1 for ciprofloxacin and ofloxacin, respectively. The outstanding regenerability and reusability of nanocomposites present tremendous potential for development of inexpensive and sustainable sorbent materials for managing pharmaceutical pollution. Literature presents scarce data and insufficient number of reports which thoroughly compares the role of differently functionalized GO to potentiate the adsorptive performance of biomass based nanocellulose and its broad application prospects in wastewater remediation. This marks the novelty of the present investigation.

A lizard with two tales: What diversification within Sceloporus occidentalis teaches us about species formation.

In 1859, Charles Darwin proposed that species are not fundamentally different from subspecies or the varieties from which they evolve. A century later, Dobzhansky (1958) suggested that many such lineages are ephemeral and are likely to reverse differentiation through introgression (Figure 1a); only a few evolve complete reproductive isolation and persist in sympatry. In this issue of Molecular Ecology, Bouzid et al. (2021) showed how new analytical methods, when applied to genomic data, allow us to more precisely determine whether or not species formation follows the paths outlined by Darwin and Dobzhansky (Figure 1b). The authors studied the diversification of the lizard Sceloporus occidentalis, finding a continuum of genetic interactions between the preservation of genetic identity to genetic merger, analogous to what is exemplified by ring species. In doing so, they teach us two tales about species formation: that lineages are fractal byproducts of evolutionary processes such as genetic drift and selection, and that lineages are often ephemeral and do not always progress into fully reproductively isolated taxa. Studying ephemeral lineages like those in S. occidentalis allows us to capture divergence at its earliest stages, and potentially to determine the factors that allow lineages to remain distinct despite pervasive gene flow. These lineages thus serve as a natural laboratory to address long standing hypotheses about species formation.

The dynamics of introgression across an avian radiation.

Hybridization and resulting introgression can play both a destructive and a creative role in the evolution of diversity. Thus, characterizing when and where introgression is most likely to occur can help us understand the causes of diversification dynamics. Here, we examine the prevalence of and variation in introgression using phylogenomic data from a large (1300+ species), geographically widespread avian group, the suboscine birds. We first examine patterns of gene tree discordance across the geographic distribution of the entire clade. We then evaluate the signal of introgression in a subset of 206 species triads using Patterson's D-statistic and test for associations between introgression signal and evolutionary, geographic, and environmental variables. We find that gene tree discordance varies across lineages and geographic regions. The signal of introgression is highest in cases where species occur in close geographic proximity and in regions with more dynamic climates since the Pleistocene. Our results highlight the potential of phylogenomic datasets for examining broad patterns of hybridization and suggest that the degree of introgression between diverging lineages might be predictable based on the setting in which they occur.

A return-on-investment approach for prioritization of rigorous taxonomic research needed to inform responses to the biodiversity crisis.

Global biodiversity loss is a profound consequence of human activity. Disturbingly, biodiversity loss is greater than realized because of the unknown number of undocumented species. Conservation fundamentally relies on taxonomic recognition of species, but only a fraction of biodiversity is described. Here, we provide a new quantitative approach for prioritizing rigorous taxonomic research for conservation. We implement this approach in a highly diverse vertebrate group-Australian lizards and snakes. Of 870 species assessed, we identified 282 (32.4%) with taxonomic uncertainty, of which 17.6% likely comprise undescribed species of conservation concern. We identify 24 species in need of immediate taxonomic attention to facilitate conservation. Using a broadly applicable return-on-investment framework, we demonstrate the importance of prioritizing the fundamental work of identifying species before they are lost.