[Homeless patients in Rotterdam with a need for medical care: data from the Rotterdam Street Doctors' office in the years 2006-2017]. / Rotterdamse daklozen met een zorgvraag.

OBJECTIVE: To describe the demographic and medical characteristics and changes of the patients who visit the Rotterdam Street Doctors' office hours. DESIGN: Retrospective study of registered patient contacts from 2006-2017. METHOD: Street doctors registered age, gender and ICPC diagnoses of patients in a GP information system. The characteristics of these patients have been analysed for three periods of four years: 2006-2009, 2010-2013, 2014-2017. For each of these periods, the number of individual patients visiting the Rotterdam Street Doctors' office hours at least once, have been documented. Data from the period 2014-2017, have been used to describe characteristics of homeless patients and have been compared with a regular GP practice. RESULTS: At the street doctors' office, patients with mental problems are most often documented, followed by patients with heart diseases and endocrine problems. Serious illnesses that require extra care are registered , such as drug and alcohol addiction, HIV, tuberculosis and hepatitis C. Patients with mental problems and trauma have a larger share at the street doctor's office than in a regular GP practice. The proportion of women has increased since 2006 as has the proportion of elderly patients. The proportion of patients with documented needs for cardiovascular diseases (in particular hypertension), endocrine disorders (in particular diabetes and thyroid diseases) and cancer has increased. CONCLUSION: A relatively large part of the patient contacts at the street doctor's office is devoted to mental problems and trauma. As the proportion of patients with chronic diseases and risk factors is rising, street doctor care seems to move towards regular care. But extra care for serious classical illnesses among the homeless is still necessary.

High PD-1 expression on regulatory and effector T-cells in lung cancer draining lymph nodes.

The treatment of advanced nonsmall cell lung cancer (NSCLC) with PD-1/PD-L1 immune checkpoint inhibitors has improved clinical outcome for a proportion of patients. The current challenge is to find better biomarkers than PD-L1 immunohistochemistry (IHC) that will identify patients likely to benefit from this therapy. In this exploratory study we assessed the differences in T-cell subsets and PD-1 expression levels on T-cells in tumour-draining lymph nodes (TDLNs) and peripheral blood mononuclear cells (PBMCs). To evaluate this, flow cytometric analyses were performed on endobronchial ultrasound-guided (EBUS) fine-needle aspirates (FNA) from TDLNs of patients with NSCLC, and the results were compared to paired PBMC samples. For a select number of patients, we were also able to obtain cells from a non-TDLN (NTDLN) sample. Our data show that the frequency of PD-1+ CD4+ and CD8+ T-cells, as well as the PD-1 expression level on activated regulatory T (aTreg) and CD4+ and CD8+ T-cells, are higher in TDLNs than in PBMCs and, in a small sub-analysis, NTDLNs. These elevated PD-1 expression levels in TDLNs may reflect tumour-specific T-cell priming and conditioning, and may serve as a predictive or early-response biomarker during PD-1 checkpoint blockade.