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Vector Borne Zoonotic Dis ; 11(7): 957-61, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21413886

ABSTRACT

We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia.


Subject(s)
Ixodes/microbiology , Rickettsia/classification , Rickettsia/genetics , Animals , Bacterial Outer Membrane Proteins/genetics , California , Databases, Nucleic Acid , Polymerase Chain Reaction , RNA , RNA, Ribosomal, 16S , Rickettsia/isolation & purification , Sequence Analysis
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