ABSTRACT
A new BODIPY dye conjugate has demonstrated selective quenching by mercury over other metal ions. Coupling of this probe to Au-Fe(3)O(4) nanoparticles as well as platinum electrodes offered sensitive systems for suspension and surface based sensing, respectively.
Subject(s)
Fluorometry , Mercury/analysis , Boron Compounds/chemistry , Color , Electrodes , Ferrosoferric Oxide/chemistry , Fluorescent Dyes/chemistry , Gold/chemistry , Hydrogen-Ion Concentration , Ions/chemistry , Metal Nanoparticles/chemistry , Platinum/chemistryABSTRACT
The zinc tank: A new fluoro-chromogenic chemosensor based on BODIPY-functionalized Fe(3)O(4) nanoparticles (1) has been prepared. Chemoprobe 1 exhibits high selectivity for Zn(2+) over other competing metal ions tested. Moreover, confocal microscopy experiments established that 1 can be used for detecting Zn(2+) levels in living cells (see figure).
Subject(s)
Ferrosoferric Oxide/chemistry , Fluorescent Dyes/chemical synthesis , Nanoparticles/chemistry , Zinc/analysis , Boron Compounds/chemistry , Fluorescent Dyes/chemistry , HeLa Cells , Humans , Molecular StructureABSTRACT
A new fluorescence receptor based on BODIPY-immobilized silica nanoparticles (BODIPY-SiO(2)) exhibits a high affinity and selectivity for Pb(2+) over competing metal ions in water. An overall emission change of ca. 100-fold at the emission maximum was observed for Pb(2+). The fluorescence receptor BODIPY-SiO(2) can remove 97% and 95% of the initial 100 ppb Pb(2+) from human blood and waste solution, respectively. Experiments show the fluorescence receptor BODIPY-SiO(2) can be a potentially useful and effective agent for the selective separation and rapid removal of Pb(2+)in vivo. We also prepared a portable chemosensor kit by coating a 4 µm thick film of BODIPY-SiO(2) onto a glass substrate. We found that this BODIPY-SiO(2) film detects Pb(2+) ions at pH 7.4 with a sensitivity of 3.2 nM. Finally, we tested the effect of pH on BODIPY-SiO(2) with Pb(2+) ions between pH 3.0 and 11.0. The fluorescence changes of BODIPY-SiO(2) were almost constant between pH 3 and 11. The results imply that the BODIPY-SiO(2) film is applicable as a portable chemosensor for detection of Pb(2+) ions in the environmental field.
Subject(s)
Biosensing Techniques/methods , Boron Compounds/chemistry , Chromatography, Thin Layer/methods , Lead/analysis , Nanoparticles/chemistry , Silicon Dioxide/chemistry , Biosensing Techniques/instrumentation , Cations, Divalent/analysis , Cations, Divalent/blood , Cations, Divalent/isolation & purification , Chromatography, Thin Layer/instrumentation , Fluorescent Dyes/chemistry , Humans , Hydrogen-Ion Concentration , Lead/blood , Lead/isolation & purification , Microscopy, Electron, Transmission , Sensitivity and Specificity , Spectrum Analysis , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/isolation & purificationABSTRACT
A new fluoro-chromogenic chemosensor based on BODIPY-functionalized Fe(3)O(4)@SiO(2) core/shell nanoparticles 1 has been prepared. Chemosensor 1 exhibits a high affinity and selectivity for Pb(2+) over competing metal ions tested. Moreover, confocal microscopy, and flow cytometry experiments established that 1 can be used for detecting Pb(2+) levels within living cell.
Subject(s)
Boron Compounds/chemistry , Lead/analysis , Microscopy, Fluorescence , Nanoparticles/chemistry , Ferrosoferric Oxide/chemistry , Flow Cytometry , HeLa Cells , Humans , Ions/chemistry , Magnetics , Silicon Dioxide/chemistryABSTRACT
A new fluoro-chromogenic chemosensor based on BODIPY-functionalized gold nanoparticles 1 is prepared. Addition of Cu(2+) ions to aqueous solutions of 1 gave an instantaneous color change along with a blue-shift of the absorption band and quenching of the emission spectrum at room temperature. The chemosensor 1 exhibits a high affinity and selectivity for Cu(2+) over competing metal ions tested. Moreover, confocal microscopy experiments establish that 1 can be used for detecting Cu(2+) levels within living cells.
