[Toxoplasma gondii infection status in abnormal pregnancy women].

OBJECTIVE: To investigate the relationship between Toxoplasma gondii (TOX) infection and abnormal pregnancy outcomes. METHODS: A total of 126 cases of abnormal pregnancy women in the Department of Obstetrics and Gynecology, the Fourth People' s Hospital of Langfang from March to December 2013 were chosen as an experimental group, and 263 cases of normal pregnancy women of childbearing age as a control group. The TOX-IgM and -IgG antibodies were detected by ELISA. The data in the two groups were processed and analyzed by SPSS13.0. RESULTS: The positive rates of TOX-IgM, -IgG in 126 cases of abnormal pregnancy women were 7.94% and 19.84% respectively, and 1.90% and 8.75% in the control group respectively, and there were significant differences between them (χ2IgM = 6.82, χ2IgG = 9.70, both P <0.01). The positive rates of TOX-IgM, -IgG in the normal pregnancy women were lower than those in 4 sub-groups of abnormal pregnancy women, and all the differences were statistically significant (χ2 spontaneous abortion = 10.40, χ2 premature delivery = 9.03 χ2 embryo damage = 4.32, χ2 birth defet = 4.04, all P< 0.05). However, the TOX-IgM, -IgG positive rates in the 4 sub-groups of abnormal pregnancy women had no statistically significant difference (P > 0.05). CONCLUSIONS: TOX infection could cause serious abnormal pregnancy outcomes. Therefore, the comprehensive control measures should be strengthened.

[Awareness and infection of Toxoplasma gondii in married childbearing women in Chengde Region].

OBJECTIVE: To understand Toxoplasma gondii infection and awareness condition of married childbearing women in Chengde Region, so as to provide the evidence for the establishment of control measures. METHODS: Totally 733 married childbearing women who took physical examination in Chengde Hospital of Traditional Chinese Medicine from July to December in 2013 were investigated by questionnaire to understand the awareness condition on T. gondii infection, then 490 women among them from 3 counties and 2 districts were randomly chosen to detect the Toxoplasma antibodies by ELISA. RESULTS: A total of 733 questionnaires were returned, and 126 women knew related knowledge about T. gondii infection, and the awareness rate was 17.19%( 126/733). Sixty-three women were determined as infected cases, and the infection rate was 12.86%( 63/490). The infection rates of the women who with higher educational level, working as medical staff and living in urban were lower, and the awareness rates of them were higher. CONCLUSIONS: The infection rate of T. gondii among the married childbearing women in Chengde Region is high, and the awareness rate of them is low. In order to decrease the infection rate as well as to increase the awareness rate of the population, the health education should be strengthened.

[Impact of Toxoplasma gondii infection on pregnancy outcomes in early pregnant women].

OBJECTIVE: To explore the impact of Toxoplasma gondii infection on pregnancy outcomes in early pregnancy women. METHODS: Toxoplasma gondii IgM and IgG antibodies in the peripheral blood of 2 993 early pregnant women were detected by using enzyme-linked immunosorbent assay (ELISA). According to the test results, the infected ones were divided into an acute infection group, a previous infection group, and an active infection group, and 200 pregnant women without Toxoplasma infection were randomly chosen as a control group, and the pregnancy outcomes of the four groups were followed up and the results were compared. RESULTS: There were 286 women infected with Toxoplasma gondii, with the infection rate of 9.56% (286/2 993), in which 43 cases were diagnosed as acute infection, 156 were previous cases, and the other 87 were active infection ones. The incidences of adverse pregnancy outcomes in the above 3 groups and the control group were 13.95% (6/43), 1.92% (3/156), 5.75% (5/87) and 1.50% (3/200), respectively. The incidences of adverse pregnancy outcomes in the acute infection group and active infection group were both higher than that in the control group, the differences were statistically significant (both P < 0.05), while there was no significant difference between the previous infection group and control group (P > 0.05). CONCLUSION: Acute and active Toxoplasma gondii infections are closely associated with the occurrence of adverse pregnancy outcomes in early pregnant women; therefore, Toxoplasma gondii IgM antibody should be included in the routine inspection items of the pre-pregnancy physical examination for child-bearing age women.

[Investigation on pregnancy outcomes and risk factors in pregnant women infected with Toxoplasma gondii].

OBJECTIVE: To understand the pregnancy outcomes and risk factors in pregnant women infected with Toxoplasma gondii. METHODS: Toxoplasma IgM and IgG antibodies in sera from 2 740 cases of pregnant women were detected by using enzyme -linked immunosorbent assay (ELISA) in Zhuozhou Municipal Maternal and Child Health Care Center from 2010 to 2013, and the pregnancy outcomes were followed up. The risk factors for Toxoplasma infection were investigated with questionnaires. RESULTS: Among the 2 740 cases of pregnant women, 195 cases were found with antibodies to T. gondii (7.12%), and among them, 44 cases were IgM positive (22.56%), and 151 cases were IgG positive (77.44%). There were 41 cases with adverse pregnancy outcomes among the 195 cases (21.02%), including 32 cases of IgM positive (78.05%) and 9 cases of IgG positive (21.95%). There were 6 cases of adverse pregnancy outcomes in uninfected pregnant women (2.86%). The difference between the two groups was statistically significant (P < 0.05). The close contact with animals, eating raw meat, eating chafing dish or barbeque, and eating raw meat stuffing were important risk factors in pregnant women infected with T. gondii (compared with the uninfected group, P < 0.01). CONCLUSION: The Toxoplasma infection may lead to adverse pregnancy outcomes, therefore, to develop good habits of life and health is an effective way to avoid adverse pregnancy outcomes.

[Efficiency of three methods for detecting Toxoplasma IgG antibody].

OBJECTIVE: To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody. METHODS: Totally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method, indirect hemagglutination test (IHA), and enzyme-linked immunosorbent assay (ELISA), and the sensitivity, specificity and Youden index of these methods were compared. RESULTS: The detection sensitivities of gold marked method, IHA, and ELISA for Toxoplasma IgG antibody were 85.5%, 89.8% and 91.9% respectively (chi2 = 4.12, P > 0.05); the specificities were 92.4%, 96.6% and 97.5% respectively (chi2 = 4.06, P > 0.05). The detection efficiency and Youden index of ELISA were 94.1% and 0.89 respectively, being higher than those of IHA and gold marked method. CONCLUSION: The sensitivity and specificity of the ELISA method for Toxoplasma IgG antibody are higher, and in addition, it can be automated. Therefore, it is suitable for large-scale Toxoplasma IgG antibody screening.

[Prevalence of IgG antibodies specific to Toxoplasma gondii among college student blood donors in Shijiazhuang City].

OBJECTIVE: To understand the epidemiological status of Toxoplasma gondii infection among blood donors in college students in Shijiazhuang City, China. METHODS: The blood samples were collected from the students in 4 universities and healthy adults in Shijiazhuang City, and the IgG antibodies specific to T. gondii were detected by using ELISA. RESULTS: The sera of 864 blood donors (422 males and 442 females) and 95 healthy adults were collected and checked for the IgG antibody titers against T. gondii. The positive rates of IgG antibody specific to T. gondii were 5.1% and 7.4% in college student blood donors and healthy adults, respectively (P < 0.05). The positive rates were not significantly different between the sexes (5.0% for the male and 5.2% for the female in blood donors of college students) and among the different universities (P > 0.05). CONCLUSION: For the higher positive rates of IgG antibody specific to T. gondii, it should be necessary to control T. gondii infection in blood donors in college students.

[Effect of gold labeling immunoassay on field screening for Toxoplasma gondii infection of blood donors].

OBJECTIVE: To evaluate the effect of gold labeling immunoassay (GLIA) on field screening for Toxoplasma gondii infection of blood donors. METHODS: A total of 1 056 specimens from the blood donors were examined by using GLIA and ELISA. RESULTS: The Toxoplasma IgG positive rates of GLIA and ELISA were 5.46% (49/898), and 4.90% (44/898), respectively, and there was no significant difference (chi2 = 0.610, P > 0.05). Those with positive results in both the two methods accounted for 4.45% (40/898), and those with negative results in both the two medhods accounted for 94.1% (845/898). The corresponding rate was 98.6% (885/898). Kappa index was 0.843, and the detection results of the two methods were consistent. CONCLUSION: The detection efficiency of GLIA is proved to be of good agreement with ELISA, and GLIA has superior features, including easy implementation and quick efficiency, so it could be used for field screening for Toxoplasma gondii infection of blood donors.

[Relationship between toxoplasma IgG and routine screening items of infectious diseases of blood donors].

Among 745 blood donors whose routine screening items were unqualified, the positive rate of Toxoplasma IgG (TOX-IgG) was 21.9%, which was higher than that of the blood donors whose routine screening items were qualified, and there was a significant difference (P < 0.01). The positive rates of TOX-IgG were 22.8% (99/435), 13.7% (16/117), 12.2% (15/123), 10.0% (5/50), 31.0% (9/29), and 34.2% (13/38) in the blood donors with abnormal ALT, positive HBsAg, positive anti-HCV, and positive anti-HIV, respectively, and all the positive rates of TOX-IgG mentioned above were higher than those in the donors whose routine screening items were qualified (P < 0.05 or P < 0.01). In conclusion, there are correlations between the positive rate of TOX-IgG and the qualified situation of routine screening items of infectious diseases in blood donors.

[Seroprevalence of Toxoplasma gondii antibodies in healthy voluntary blood donors from Shijiazhuang area].

The positive rate of Toxoplasma gondii antibodies in healthy voluntary blood donors in Shijiazhuang area was 4.83%, and it showed differences among the different regions, occupations, ages, education levels and nations. So the blood donors should receive the Toxoplasma infection screening in order to prevent toxoplasmosis from blood recipients.