Long-term evaluating the strengthening effects of iron-carbon mediator for coking wastewater treatment in EGSB reactor.

Coking wastewater (CWW) treatment is difficult due to its complex composition and high biological toxicity. Iron-carbon mediators was used to enhance the treatment of CWW through iron-carbon microelectrolysis (ICME). The results indicated that the removal rate of COD and phenolic compounds were enhanced by 24.1 % and 23.5 %, while biogas production and methane content were promoted by 50 % and 7 %. Microbial community analysis indicated that iron-carbon mediators had a transformative impact on the reactor's performance and dependability by enriching microorganisms involved in direct and indirect electron transfer, such as Anaerolineae and Methanothrix. The mediator also produced noteworthy gains in LB-EPS and TB-EPS, increasing by roughly 109.3 % and 211.6 %, respectively. PICRISt analysis demonstrated that iron-carbon mediators effectively augment the abundance of functional genes associated with metabolism, Citrate cycle, and EET pathway. This study provides a new approach for CWW treatment.

Chemotherapy-initiated cysteine-rich protein 61 decreases acute B-lymphoblastic leukemia chemosensitivity.

PURPOSE: Chemoresistance is a major challenge for acute lymphoblastic leukemia (ALL) treatment. Cysteine-rich protein 61 (Cyr61) plays an important role in drug resistance modulation of tumor cells, and Cyr61 levels are increased in the bone marrow of patients with ALL and contribute to ALL cell survival. However, the effect of Cyr61 on B cell acute lymphoblastic leukemia (B-ALL) cell chemosensitivity and the regulatory mechanisms underlying Cyr61 production in bone marrow remain unknown. METHODS: Nalm-6 and Reh human B-ALL cell lines were used in this study. Cyr61 levels were assessed using quantitative real-time PCR (qRT-PCR), western blot analysis, and enzyme-linked immunosorbent assay. The effect of Cyr61 on B-ALL cell chemosensitivity to daunorubicin (DNR) was evaluated using cell viability and flow cytometry analyses. The regulatory mechanisms of Cyr61 production in bone marrow were examined using qRT-PCR and western blot analysis. RESULTS: Cyr61 knockdown and overexpression increased and decreased the chemosensitivity of B-ALL cells to DNR, respectively. Cyr61 attenuated chemotherapeutic drug-induced apoptosis by upregulating B cell lymphoma-2. Notably, DNR induced DNA damage response and increased Cyr61 secretion in B-ALL cells through the ataxia telangiectasia mutated (ATM)-dependent nuclear factor kappa B pathway. CONCLUSION: DNR induces Cyr61 production in B-ALL cells, and increased Cyr61 levels reduce the chemosensitivity of B-ALL cells. Consequently, targeting Cyr61 or related ATM signaling pathway may present a promising treatment strategy to enhance the chemosensitivity of patients with B-ALL.

Nitrogen-Doped Titanium Dioxide for Selective Photocatalytic Oxidation of Methane to Oxygenates.

Photocatalytic conversion of methane (CH4) to value-added chemicals using H2O as the oxidant under mild conditions is a desired sustainable pathway for synthesizing commodity chemicals. However, controlling product selectivity while maintaining high product yields is greatly challenging. Herein, we develop a highly efficient strategy, based on the precise control of the types of nitrogen dopants, and the design of photocatalysts, to achieve high selectivity and productivity of oxygenates via CH4 photocatalytic conversion. The primary product (methanol) is obtained in a high yield of 159.8 µmol·g-1·h-1 and 47.7% selectivity, and the selectivity of oxygenate compounds reached 92.5%. The unique hollow porous structure and substituted nitrogen sites of nitrogen-doped TiO2 synergistically promote its photo-oxidation performance. Furthermore, in situ attenuated total reflectance Fourier transform infrared spectroscopy provides direct evidence of the key intermediates and their evolution for producing methanol and multicarbon oxygenates. This study provides insights into the mechanism of photocatalytic CH4 conversion.

Electrochemical Epoxidation of Propylene to Propylene Oxide via Halogen-Mediated Systems.

As one of the most important derivatives of propylene, the production of propylene oxide (PO) is severely restricted. The traditional chlorohydrin process is being eliminated due to environmental concerns, while processes such as Halcon and hydrogen peroxide epoxidation are limited by cost and efficiency, making it difficult to meet market demand. Therefore, achieving PO production through clean and efficient technologies has received extensive attention, and halogen-mediated electrochemical epoxidation of alkene is considered to be a desirable technology for the production of alkylene oxide. In this work, we used electrochemical methods to synthesize PO in halogen-mediated systems based on a RuO2-loaded Ti (RuO2/Ti) anode and screened out two potential mediated systems of chlorine (Cl) and bromine (Br) for the electrosynthesis of PO. At a current density of 100 mA·cm-2, both Cl- and Br-mediated systems delivered PO Faradaic efficiencies of more than 80%. In particular, the Br-mediated system obtained PO Faradaic efficiencies of more than 90% at lower potentials (≤1.5 V vs RHE) with better electrode structure durability. Furthermore, detailed product distribution investigations and DFT calculations suggested hypohalous acid molecules as key reaction intermediates in both Cl- and Br-mediated systems. This work presents a green and efficient PO production route with halogen-mediated electrochemical epoxidation of propylene driven by renewable electricity, exhibiting promising potential to replace the traditional chlorohydrin process.

Case Report: A novel TP53 mutation in a patient with quadruple wild-type gastrointestinal stromal tumor.

In this report, we present a case study of a 64-year-old female who was diagnosed with gastrointestinal stromal tumors (GISTs) and subsequently developed liver metastases despite undergoing radical resection. Next-generation sequencing (NGS) assays indicated that the tumor lacked KIT/PDGFRA/SDH/RAS-P (RAS pathways, RAS-P) mutations, thereby classifying this patient as quadruple WT GIST (qGIST). Treatment with imatinib was initiated, and after 2.5 months, recurrence of the tumor and multiple metastases around the surgical site were observed. Consequently, the patient was switched to sunitinib treatment and responded well. Although she responded well to sunitinib, the patient died of tumor dissemination within 4 months. This case study highlights the potential efficacy of imatinib and the VEGFR-TKI sunitinib in treating qGIST patients harboring a TP53 missense mutation.

Evaluating the stability and performance of a novel core-shell ZVI@C-montmorillonite particle for anaerobic treatment of chloramphenicol wastewater.

ZVI@C-MP is a novel composite particle consisting of zero-valent iron (ZVI) enclosed within a carbon shell. The purpose of this composite material is to enhance the anaerobic treatment of wastewater containing chloramphenicol (CAP). This approach aims to address the initial challenge of excessive corrosion experienced by ZVI, followed by its subsequent passivation and inactivation. ZVI@C-MP was synthesized through a hydrothermal process and calcination, with montmorillonite as binder, it exhibits stability, iron-carbon microelectrolysis (ICME) properties, and strong adsorption for CAP. Its ICME actions include releasing iron ions (0.70 mg/L) and COD (11.3 mg/L), generating hydrogen (3.82%), and raising the pH from 6.30 to 7.71. With minimal structural changes, it achieved release equilibrium. ZVI@C-MP boasts high removal efficiency of CAP (98.96%) by adsorption, attributed to surface characteristics (surface area: 167.985 m2/g; pore volume: 0.248 cm3/g). The addition of ZVI@C-MP increases COD removal (10.16%), methane production (72.86%), and reduces extracellular polymeric substances (EPS) from 70.58 to 52.72 mg/g MLVSS. It reduces microbial by-products and toxic effects, enhancing CAP biodegradation and microbial metabolic activity. ZVI@C-MP's electrical conductivity and biocompatibility bolster functional flora for interspecies electron transfer. It's a novel approach to antibiotic wastewater treatment.

Three-dimensional graphene aerogel mitigated the toxic impact of chloramphenicol wastewater on microorganisms in an EGSB reactor.

Anaerobic treatment of chloramphenicol wastewater holds significant promise due to its potential for bioenergy generation. However, the high concentration of organic matter and residual toxic substances in the wastewater severely inhibit the activity of microorganisms. In this study, a three-dimensional graphene aerogel (GA), as a conductive material with high specific surface area (114.942 m2 g-1) and pore volume (0.352 cm3 g-1), was synthesized and its role in the efficiency and related mechanism for EGSB reactor to treat chloramphenicol wastewater was verified. The results indicated that synergy effects of GA for Chemical Oxygen Demand (COD) removal (increased by 8.17 %), chloramphenicol (CAP) removal (increased by 4.43 %) and methane production (increased by 70.29 %). Furthermore, GA increased the average particle size of anaerobic granular sludge (AGS) and promoted AGS to secrete more redox active substances. Microbial community analysis revealed that GA increased the relative abundance of functional bacteria and archaea, specifically Syntrophomonas, Geobacter, Methanothrix, and Methanolinea. These microbial species can participate in direct interspecific electron transfer (DIET). This research serves as a theoretical foundation for the application of GA in mitigating the toxic impact of refractory organic substances, such as antibiotics, on microorganisms during anaerobic treatment processes.

Potential benefits and risks of solar photovoltaic power plants on arid and semi-arid ecosystems: an assessment of soil microbial and plant communities.

Exponential increase in photovoltaic installations arouses concerns regarding the impacts of large-scale solar power plants on dryland ecosystems. While the effects of photovoltaic panels on soil moisture content and plant biomass in arid ecosystems have been recognized, little is known about their influence on soil microbial communities. Here, we employed a combination of quantitative PCR, high-throughput sequencing, and soil property analysis to investigate the responses of soil microbial communities to solar panel installation. We also report on the responses of plant communities within the same solar farm. Our findings showed that soil microbial communities responded differently to the shading and precipitation-alternation effects of the photovoltaic panels in an arid ecosystem. By redirecting rainwater to the lower side, photovoltaic panels stimulated vegetation biomass and soil total organic carbon content in the middle and in front of the panels, positively contributing to carbon storage. The shade provided by the panels promoted the co-occurrence of soil microbes but inhibited the abundance of 16S rRNA gene in the soil. Increase in precipitation reduced 18S rRNA gene abundance, whereas decrease in precipitation led to decline in plant aboveground biomass, soil prokaryotic community alpha diversity, and dehydrogenase activity under the panels. These findings highlight the crucial role of precipitation in maintaining plant and soil microbial diversities in dryland ecosystems and are essential for estimating the potential risks of large-scale solar power plants on local and global climate change in the long term.

Highly Efficient CO2 Reduction at Steady 2 A cm-2 by Surface Reconstruction of Silver Penetration Electrode.

Electroreduction of CO2 to CO is a promising route for greenhouse gas resource utilization, but it still suffers from impractical current density and poor durability. Here, a nanosheet shell (NS) vertically standing on the Ag hollow fiber (NS@Ag HF) surface formed by electrochemical surface reconstruction is reported. As-prepared NS@Ag HF as a gas penetration electrode exhibited a high CO faradaic efficiency of 97% at an ultra-high current density of 2.0 A cm-2 with a sustained performance for continuous >200 h operation. The experimental and theoretical studies reveal that promoted surface electronic structures of NS@Ag HF by the nanosheets not only suppress the competitive hydrogen evolution reaction but also facilitate the CO2 reduction kinetics. This work provides a feasible strategy for fabricating robust catalysts for highly efficient and stable CO2 reduction.

Ni Nanoclusters Anchored on Ni-N-C Sites for CO2 Electroreduction at High Current Densities.

Transition metal catalyst-based electrocatalytic CO2 reduction is a highly attractive approach to fulfill the renewable energy storage and a negative carbon cycle. However, it remains a great challenge for the earth-abundant VIII transition metal catalysts to achieve highly selective, active, and stable CO2 electroreduction. Herein, bamboo-like carbon nanotubes that anchor both Ni nanoclusters and atomically dispersed Ni-N-C sites (NiNCNT) are developed for exclusive CO2 conversion to CO at stable industry-relevant current densities. Through optimization of gas-liquid-catalyst interphases via hydrophobic modulation, NiNCNT exhibits as high as Faradaic efficiency (FE) of 99.3% for CO formation at a current density of -300 mA·cm-2 (-0.35 V vs reversible hydrogen electrode (RHE)), and even an extremely high CO partial current density (jCO) of -457 mA·cm-2 corresponding to a CO FE of 91.4% at -0.48 V vs RHE. Such superior CO2 electroreduction performance is ascribed to the enhanced electron transfer and local electron density of Ni 3d orbitals upon incorporation of Ni nanoclusters, which facilitates the formation of the COOH* intermediate.

[Effect of Cyr61 on Imatinib Resistance in Chronic Myeloid Leukemia and Its Mechanism].

OBJECTIVE: To investigate the effect of Cyr61 on imatinib (IM) resistance in chronic myeloid leukemia (CML) and its mechanism. METHODS: Cyr61 level in cell culture supernatant was determined by enzyme-linked immunosorbent assay. The expression of Cyr61 and Bcl-xL were measured by real-time PCR and Western blot. Cell apoptosis was analyzed using an Annexin V-APC Kit. Expression of signal pathways related proteins was determined by Western blot. RESULTS: The level of Cyr61 obviously increased in K562G cells (IM resistance to CML cell line K562). Down-regulating the expression of Cyr61 decreased the resistance of K562G cells to IM and promoted IM induced apoptosis. In CML mouse model, down-regulating the expression of Cyr61 could increase the sensitivity of K562G cells to IM. The mechanism studies showed that Cyr61 mediated IM resistance in CML cells was related to the regulation of ERK1/2 pathways and apoptosis related molecule Bcl-xL by Cyr61. CONCLUSION: Cyr61 plays an important role in promoting IM resistance of CML cells. Targeting Cyr61 or its related effectors pathways may be one of the ways to overcome IM resistance of CML cells.

Highly Selective Photoelectroreduction of Carbon Dioxide to Ethanol over Graphene/Silicon Carbide Composites.

Using sunlight to produce valuable chemicals and fuels from carbon dioxide (CO2 ), i.e., artificial photosynthesis (AP) is a promising strategy to achieve solar energy storage and a negative carbon cycle. However, selective synthesis of C2 compounds with a high CO2 conversion rate remains challenging for current AP technologies. We performed CO2 photoelectroreduction over a graphene/silicon carbide (SiC) catalyst under simulated solar irradiation with ethanol (C2 H5 OH) selectivity of>99 % and a CO2 conversion rate of up to 17.1 mmol gcat -1 h-1 with sustained performance. Experimental and theoretical investigations indicated an optimal interfacial layer to facilitate the transfer of photogenerated electrons from the SiC substrate to the few-layer graphene overlayer, which also favored an efficient CO2 to C2 H5 OH conversion pathway.

Comparison between Clinical Utility of CXCL-8 and Clinical Practice Tumor Markers for Colorectal Cancer Diagnosis.

Owing to the high incidence and mortality rates of colorectal cancer (CRC), novel biomarkers for CRC diagnosis are critically needed. Therefore, this study is aimed at exploring the clinical utility of serum C-X-C motif chemokine 8 (CXCL-8) for CRC diagnosis and progression compared to the routinely used biomarkers, carcinoembryonic antigen (CEA), and carbohydrate antigen-19-9 (CA19-9). This study included 227 patients with CRC, 110 patients with colorectal adenoma (CA), and 123 healthy participants, who were recruited from the Fujian Medical University Union Hospital from July 1, 2019 to October 31, 2020. Serum concentrations of CXCL-8, CEA, and CA19-9 were detected using enzyme-linked immunosorbent assay and chemiluminescent microparticle immunoassay. Clinicopathological features of patients with CRC were collected and analyzed. The diagnostic efficacy of CXCL-8, CEA, and CA19-9 for CRC was evaluated using receiver operating characteristic (ROC) curves. We found that the serum concentrations of CXCL-8, CEA, and CA19-9 were significantly higher in patients with CRC than those in patients with CA and healthy controls. The diagnostic sensitivity of CXCL-8 alone was higher than those of CEA and CA19-9 both and when combined; thus, CXCL-8 may be better at discriminating patients with CRC from healthy controls and patients with CA. Moreover, combining CXCL-8 with CEA or CA19-9 improved their respective diagnostic performances in distinguishing patients with CRC from CA patients and healthy participants. Notably, we also found that serum concentrations of CXCL-8 were positively correlated with metastases and tumor size. Therefore, our study suggests that serum CXCL-8 may serve as an improved biomarker for CRC diagnosis compared to the traditional tumor markers CEA and CA19-9. Moreover, our findings indicate the potential efficacy of serum CXCL-8 levels as a CRC prognostic biomarker.

Chloride Ion Adsorption Enables Ampere-Level CO2 Electroreduction over Silver Hollow Fiber.

Electrochemical conversion of CO2 into valuable feedstocks is a promising strategy for carbon neutrality. However, it remains a challenge to possess a large current density, a high faradaic efficiency and excellent stability for practical applications of CO2 utilization. Herein, we report a facile tactic that enables exceedingly efficient CO2 electroreduction to CO by virtue of low-coordination chloride ion (Cl- ) adsorption on a silver hollow fiber (Ag HF) electrode. A CO faradaic efficiency of 92.3 % at a current density of one ampere per square centimeter (1 A cm-2 ) in 3.0 M KCl with a sustained performance observed during a 150-hour test was achieved, which is better than state-of-the-art electrocatalysts. The electrochemical results and density functional theory (DFT) calculations suggested a low-coordination Cl- adsorption on surface of Ag HF, which not only suppressed the competitive hydrogen evolution reaction (HER), but also facilitated the CO2 reduction kinetics.

Methylated Septin 9 as a Promising Biomarker in the Diagnosis and Recurrence Monitoring of Colorectal Cancer.

Purpose: The clinical utility of plasma methylated septin 9 (mSEPT9) DNA in screening and recurrence monitoring for colorectal cancer (CRC) is highly promising. The present study was performed to determine the diagnostic value of mSEPT9 in CRC detection and recurrence monitoring in Chinese patients. Methods: Overall, 616 patients newly diagnosed with CRC and 122 individuals with no evidence of disease were recruited from October 1, 2019, to May 31, 2021, at Fujian Medical University Union Hospital. Plasma and serum samples were collected for analyzing mSEPT9, carcinoembryonic antigen (CEA), and carbohydrate antigen-19-9 (CA19-9). Data on clinicopathological characteristics were collected and analyzed. Sensitivity and specificity were calculated to evaluate the diagnostic potential of each marker; the receiver operating characteristic (ROC) curve was applied for the assessment of diagnostic value, and comparisons among mSEPT9, CEA, CA19-9, and their combination were assessed via ROC curves. Results: mSEPT9 achieved an overall sensitivity and specificity of 72.94% and 81.97%, respectively, with an area under the curve (AUC) value of 0.826, which were higher than those of CEA (sensitivity: 43.96%; specificity: 96.72%; AUC: 0.789) and CA19-9 (sensitivity: 14.99%; specificity: 96.61%; AUC: 0.590). The combination of mSEPT9, CEA, and CA19-9 further improved sensitivity, specificity, and AUC value (sensitivity: 78.43%; specificity: 86.07%; AUC: 0.878), respectively. Notably, the mSEPT9 positivity rate was significantly associated with TNM stage, T stage, N stage, tumor size, vascular invasion, and nerve invasion among patients with CRC. A 100% correlation was observed between the positive results of the mSEPT9 test and recurrence or metastasis in patients after therapeutic intervention. Conclusion: Our findings suggest that mSEPT9 may represent a potential biomarker for the diagnosis and prognosis of CRC compared with CEA and CA19-9. Postoperative mSEPT9 status may represent the first noninvasive marker of CRC recurrence or metastasis.

Effectiveness of Trastuzumab Combined With Capecitabine Treatment in a Patient With Hilar Cholangiocarcinoma Complicated by Liver Metastases With an ERBB2-Activating Mutation: A Case Report.

The identification of ERBB2 (HER2) alteration in some solid tumors has become critically important due to the actionable events predictive of response to anti-HER2 therapy. However, the efficacy of ERBB2 mutated hilar cholangiocarcinoma (hCCA) against ERBB2 is rarely reported. Here we report a 76-year-old female diagnosed with hCCA complicated by liver metastases after radical resection. The next-generation sequencing assay showed that the tumor had an ERBB2 mutation. Then, the patient was treated with trastuzumab plus capecitabine. After 2 months of treatment, she had a partial response. Until now, the patient is still alive. This study has shown the potential of trastuzumab combined with capecitabine as an effective treatment for hilar cholangiocarcinoma complicated by liver metastases harboring ERBB2 alterations.

[Primary culture and identification of glomerular mesangial cells from mice with spontaneous lupus nephritis].

Objective To perform primary culture of glomerular mesangial cells, identify them from a mouse model of spontaneous lupus nephritis model and explore the optimal conditions for these procedures. Methods Nine control ICR mice and nine model MRL/lpr mice with spontaneous lupus nephritis were tested for autoantibodies using ELISA and urine protein was detected by bicinchoninic acid (BCA) assay at 12, 14 and 18 weeks of age. HE and Masson staining were used to observe the pathological changes of mice with lupus nephritis. Glomeruli were isolated using a cell sieve, while primary culture of glomerular mesangial cells was performed using the eugenic selection method. Glomerular mesangial cells of the MRL/lpr mice were identified based on cell morphology and immunocytochemical staining of α-smooth muscle actin and nephrin. Results In the model group, levels of anti-nuclear antibody and anti-double-stranded DNA antibodies significantly increased. Urine protein levels also increased significantly at 14 and 18 weeks of age. HE and masson staining of kidney tissue revealed pathological changes associated with lupus nephritis in the MRL/lpr mice from 12 weeks of age. Twenty-five days after seeding of the glomerular mesangial cells, those from the MRL/lpr mice gradually covered the bottom of the culture flasks, appearing star-shaped and fusiform by phase contrast microscopy. Immunocytochemistry showed that these cells were α-smooth muscle actin-positive and nephrin-negative, thus ruling out other glomerular intrinsic cells. Conclusion The primary culture of glomerular mesangial cells from mice with spontaneous lupus nephritis is developed and characterized.

Hierarchical micro/nanostructured silver hollow fiber boosts electroreduction of carbon dioxide.

Efficient conversion of CO2 to commodity chemicals by sustainable way is of great significance for achieving carbon neutrality. Although considerable progress has been made in CO2 utilization, highly efficient CO2 conversion with high space velocity under mild conditions remains a challenge. Here, we report a hierarchical micro/nanostructured silver hollow fiber electrode that reduces CO2 to CO with a faradaic efficiency of 93% and a current density of 1.26 A · cm-2 at a potential of -0.83 V vs. RHE. Exceeding 50% conversions of as high as 31,000 mL · gcat-1 · h-1 CO2 are achieved at ambient temperature and pressure. Electrochemical results and time-resolved operando Raman spectra demonstrate that enhanced three-phase interface reactions and oriented mass transfers synergistically boost CO production.

Factors driving the assembly of prokaryotic communities in bulk soil and rhizosphere of Torreya grandis along a 900-year age gradient.

Excessive nutrient inputs imperil the stability of forest ecosystems via modifying the interactions among soil properties, microbes, and plants, particularly in forests composed of cash crops that are under intensive disturbances of agricultural activities, such as Torreya grandis. Understanding the potential drivers of soil microbial community helps scientists develop effective strategies for balancing the protection and productivity of the ancient Torreya forest. Here, we assayed the link between plant and soil parameters and prokaryote communities in bulk soil and T. grandis rhizosphere in 900-year-old stands by detecting plant and soil properties in two independent sites in southeastern China. Our results showed no apparent influence of stand age on the compositions of prokaryote communities in bulk soil and T. grandis rhizosphere. In contrast, soil abiotic factors (i.e., soil pH) overwhelm plant characteristics (i.e., height, plant tissue carbon, nitrogen, and phosphorus content) and contribute most to the shift in prokaryote communities in bulk soil and T. grandis rhizosphere. Soil pH leads to an increase in microbiota alpha diversity in both compartments. With the help of a random forest, we found a critical transition point of pH (pH = 4.9) for the dominance of acidic and near-neutral bacterial groups. Co-occurrence network analysis further revealed a substantially simplified network in plots with a pH of <4.9 versus samples with a pH of ≥4.9, indicating that soil acidification induces biodiversity loss and disrupts potential interactions among soil microbes. Our findings provide empirical evidence that soil abiotic properties nearly completely offset the roles of host plants in the assembly and potential interactions of rhizosphere microorganisms. Hence, reduction in inorganic fertilization and proper liming protocols should be seriously considered by local farmers to protect ancient Torreya forests.

Serum CYR61 as a potential biomarker to improve breast cancer diagnostics.

Purpose: To investigate the role of serum CYR61 as a biomarker for the diagnosis of breast cancer and to analyze the association between serum CYR61 levels and the clinicopathological features in breast cancer patients. Methods: Serum CYR61 was measured in breast cancer patients and healthy controls by ELISA. Results: The serum levels of CYR61 in breast cancer patients were higher than those in healthy controls. The area under the receiver operating characteristic curve for CYR61 was higher than that for carcinoembryonic antigen and carbohydrate antigen 15-3. The increased CYR61 levels were correlated with menopausal status and Ki67 expression. Conclusion: Serum CYR61 might be a novel biomarker to assist the diagnosis and clinicopathological status assessment of breast cancer.