ABSTRACT
OBJECTIVE: To clarify the association between IL-8 gene polymorphisms, IL-8 level, towards the risk of age-related macular degeneration (AMD). METHODS: Meta-analysis was performed from available studies that investigated IL-8 -251A/T (rs4073) and +781C/T (rs2227306) polymorphisms and IL-8 levels in patients with AMD and controls. RESULTS: Overall, the pooled result showed a significant association between AMD with allelic (T vs. C; OR 1.53; pâ¯=â¯0.005), dominant (TTâ¯+â¯CT vs. CC; OR 1.95; pâ¯=â¯0.017), homozygous (TT vs. CC; OR 2.03; pâ¯=â¯0.039) and heterozygous (CT vs. CC; OR 1.92; pâ¯=â¯0.032) models of rs2227306; while subgroup analysis revealed a significant association between rs2227306 with wet AMD in allelic (T vs. C; OR 1.69; pâ¯=â¯0.016), recessive (TT vs. CTâ¯+â¯CC; OR 1.81; pâ¯=â¯0.00007), and homozygous (TT vs. CC; OR 2.64; pâ¯=â¯0.003) models. No significant association was observed between rs4073 with AMD in all inheritance models. In parallel, patients with AMD, particularly wet AMD had an elevated level of IL-8 compared to control. CONCLUSION: This meta-analysis suggests that patients with AMD or wet AMD have higher IL-8 levels compared to control, which is also supported by the evidence that carrier T allele of rs2227306 exhibited an increase in the risk of AMD or wet AMD. Thus, IL-8 +781C/T (rs2227306) polymorphism and the level of intraocular IL-8 may be useful as a biomarker for early detection and a therapeutic target of AMD.
Subject(s)
Interleukin-8/genetics , Wet Macular Degeneration , Alleles , Homozygote , Humans , Polymorphism, Single Nucleotide , Wet Macular Degeneration/geneticsABSTRACT
Objetivo Aclarar la asociación entre polimorfismos del gen IL-8 y nivel de IL-8 en el riesgo de degeneración macular asociada a la edad (DMAE). Métodos Se realizó un metaanálisis a partir de los estudios disponibles que investigaron los polimorfismos de IL-8 −251A/T (rs4073) y +781C/T (rs2227306), y niveles de IL-8, en pacientes con DMAE y controles. Resultado En total, el resultado agrupado mostró una asociación significativa entre DMAE con modelos alélicos (T vs. C; OR 1,53; p = 0,005), dominantes (TT + CT vs. CC; OR 1,95; p = 0,017), homocigotos (TT vs. CC; OR 2,03; p = 0,039) y heterocigotos (CT vs. CC; OR 1,92; p = 0,032) de rs2227306; mientras que el análisis de subgrupos reveló una asociación significativa entre rs2227306 con DMAE húmedo en modelos alélicos (T vs. C; OR 1,69; p = 0,016), recesivos (TT vs. CT + CC; OR 1,81; p = 0,00007), y homocigotos (TT vs. CC; OR 2,64; p = 0,003). No se observó asociación significativa entre rs4073 con DMAE en todos los modelos de herencia. Paralelamente, los pacientes con DMAE, particularmente DMAE húmedo, tenían un nivel elevado de IL-8 en comparación con el control. Conclusión Este metaanálisis sugiere que los pacientes con DMAE o DMAE húmedo tienen niveles más altos de IL-8 en comparación con controles, lo que también está respaldado por la evidencia de que el alelo T portador de rs2227306 exhibe un aumento en el riesgo de DMAE o DMAE húmedo. Por lo tanto, el polimorfismo IL-8 +781C/T (rs2227306) y el nivel de IL-8 intraocular pueden ser útiles como biomarcadores para la detección temprana y objetivo terapéutico de DMAE (AU)
Objective To clarify the association between IL-8 gene polymorphisms, IL-8 level, towards the risk of age-related macular degeneration (AMD). Methods Meta-analysis was performed from available studies that investigated IL-8 −251A/T (rs4073) and +781C/T (rs2227306) polymorphisms and IL-8 levels in patients with AMD and controls. Result Overall, the pooled result showed a significant association between AMD with allelic (T vs. C; OR 1.53; p = 0.005), dominant (TT + CT vs. CC; OR 1.95; p = 0.017), homozygous (TT vs. CC; OR 2.03; p = 0.039) and heterozygous (CT vs. CC; OR 1.92; p = 0.032) models of rs2227306; while subgroup analysis revealed a significant association between rs2227306 with wet AMD in allelic (T vs. C; OR 1.69; p = 0.016), recessive (TT vs. CT + CC; OR 1.81; p = 0.00007), and homozygous (TT vs. CC; OR 2.64; p = 0.003) models. No significant association was observed between rs4073 with AMD in all inheritance models. In parallel, patients with AMD, particularly wet AMD had an elevated level of IL-8 compared to control. Conclusion This meta-analysis suggests that patients with AMD or wet AMD have higher IL-8 levels compared to control, which is also supported by the evidence that carrier T allele of rs2227306 exhibited an increase in the risk of AMD or wet AMD. Thus, IL-8 +781C/T (rs2227306) polymorphism and the level of intraocular IL-8 may be useful as a biomarker for early detection and a therapeutic target of AMD (AU)
Subject(s)
Humans , Macular Degeneration/genetics , Interleukin-8/genetics , Polymorphism, Single Nucleotide/genetics , Risk FactorsABSTRACT
OBJECTIVE: To determine the change in aqueous humor interleukin-6 (IL-6) levels among primary open-angle glaucoma (GPAA) patients. METHODS: Studies that investigated IL-6 level in the aqueous humor in GPAA patients using the detección methods of enzyme-linked immunosorbent assay (ELISA) y multiplex bead immunoassay were retrieved. A meta-analysis was performed a determine the overall change in IL-6 of GPAA patients compared a controls. RESULTS: A total of 14 studies were selected. Analysis combining the results of studies using both detección approaches did not show any changes in the level of IL-6 in GPAA (SMD=-0,07, 95%IC -0,73 - 0,59, P=0,83), possibly due a the heterogeneity of ELISA data. Since multiplex bead immunoassay is more sensitive than convenciónal ELISA in detecting antibodies, further isolated analysis of multiplex bead immunoassay results revealed that GPAA patients had a lower level of IL-6 in the aqueous humor compared a controls (SMD=-0,40, 95%IC -0,70 - 0,09, P=0,01). Moreover, a sensitivity test also confirmed that no alteracións of results were observed in all pooled studies y pooled studies from multiplex bead immunoassay, suggesting the stabilities of our synthetic results. CONCLUSION: Because of its robustness, the pooled data from multiplex bead immunoassay was used a draw a conclusion in this study, showing that the reducción of IL-6 levels in aqueous humor was observed in patients with GPAA. Further studies are still warranted a confirm our findings.
Subject(s)
Aqueous Humor/chemistry , Glaucoma, Open-Angle/metabolism , Interleukin-6/analysis , Enzyme-Linked Immunosorbent Assay , Humans , Immunomagnetic Separation , Interleukin-6/physiology , Reproducibility of Results , Sensitivity and Specificity , Signal Transduction , Transforming Growth Factor beta/metabolismABSTRACT
Previous studies have demonstrated that female patients with coronavirus disease 2019 (COVID-19) demonstrate more favorable prognosis relative to male patients. In this article, we elaborate the possible role of estrogen in the modulation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection severity. The potential interplay between several factors, including inherently lower estradiol (E2) and slightly higher estrogen receptor ß (ERß) levels in males, with inflammatory mediators are described. Altogether, there seems to be a sexually dimorphic response towards SARS-CoV-2 infection, and a possibility that COVID-19 severity is dependent on both E2 levels and ERα:ERß expression ratio in lymphoid and lung cells.
