ABSTRACT
A truncated form of the ATP release channel pannexin 1 (Panx1), Panx1 1-89 , is enriched in metastatic breast cancer cells and has been proposed to mediate metastatic cell survival by increasing ATP release through mechanosensitive Panx1 channels. However, whether Panx1 1-89 on its own (without the presence of wtPanx1) mediates ATP release has not been tested. Here, we show that Panx1 1-89 by itself can form a constitutively active membrane channel, capable of releasing ATP even in the absence of wild type Panx1. Our biophysical characterization reveals that most basic structure-function features of the channel pore are conserved in the truncated Panx1 1-89 peptide. Thus, augmenting extracellular potassium ion concentrations enhances Panx1 1-89 -mediated conductance. Moreover, despite the severe truncation, Panx1 1-89 retains the sensitivity to most of wtPanx1 channel inhibitors and can thus be targeted. Therefore, Panx1 blockers have the potential to be of therapeutic value to combat metastatic cell survival. Our study not only elucidates a mechanism for ATP release from cancer cells, but it also supports that the Panx1 1-89 mutant should facilitate structure-function analysis of Panx1 channels.
ABSTRACT
En este trabajo se describen las características generales del sistema de salud de Bolivia: su organización y cobertura; sus fuentes de financiamiento y gasto en salud; los recursos físicos, materiales y humanos de los que dispone; las actividades de rectoría que desarrolla, y el nivel que ha alcanzado la investigación en salud. También se discuten las innovaciones más recientes que se han llevado a cabo en los últimos años, incluyendo el Seguro Universal Materno Infantil, el Programa de Extensión de Cobertura a Áreas Rurales, el Modelo de Salud Familiar, Comunitaria e Intercultural y el programa de subsidios monetarios Juana Azurduy, dirigido a fortalecer la atención prenatal y del parto.
This paper describes the Bolivian health system, including its structure and organization, its financing sources, its health expenditure, its physical, material and humans resources, its stewardship activities and the its health research institutions. It also discusses the most recent policy innovations developed in Bolivia: the Maternal and Child Universal Insurance, the Program for the Extension of Coverage to Rural Areas, the Family, Community and Inter-Cultural Health Model and the cash-transfer program Juana Azurduy intended to strengthen maternal and child care.
Subject(s)
Humans , Delivery of Health Care/organization & administration , Health Services Administration , Bolivia , Community Participation/statistics & numerical data , Delivery of Health Care/economics , Delivery of Health Care/statistics & numerical data , Demography , Financing, Organized/economics , Financing, Organized/organization & administration , Financing, Organized/statistics & numerical data , Government Programs/economics , Government Programs/organization & administration , Government Programs/statistics & numerical data , Health Expenditures/statistics & numerical data , Health Resources/organization & administration , Health Resources/statistics & numerical data , Health Resources/supply & distribution , Health Services Administration/economics , Health Services Administration/statistics & numerical data , Health Services/economics , Health Services/statistics & numerical data , Health Status Indicators , Insurance Benefits/economics , Insurance Benefits/statistics & numerical data , Insurance Coverage/economics , Insurance Coverage/statistics & numerical data , Insurance, Health/economics , Insurance, Health/organization & administration , Insurance, Health/statistics & numerical data , National Health Programs/economics , National Health Programs/organization & administration , National Health Programs/statistics & numerical data , Organizational Innovation , Private Sector/economics , Private Sector/organization & administration , Private Sector/statistics & numerical data , Quality Assurance, Health Care/organization & administration , Social Security/economics , Social Security/organization & administration , Social Security/statistics & numerical data , Vital StatisticsABSTRACT
Se presentan seis informes sobre el avance del Proyecto Dots Universitario , de la red de salud sur, perteneciente al mes de marzo