Prediction of protein architectures involved in the signaling-pathway initiating sporulation in Firmicutes.

OBJECTIVES: Like many other proteins, those belonging to the signal transduction cascade initiating sporulation (Spo0 pathway) have conserved protein domains (Capra and Laub in Annu Rev Microbiol 66:325-47, 2012). Improvements in bioinformatics applications to discover proteins involved in the initiation of the sporulating cascade in newly sequenced genomes is an important task that requires rigorous comparative genomic methods and manual curation to identify endospore-forming bacteria. This note aims to present a collection of predicted proteins involved in the Spo0 pathway found in the proteomes of fully sequenced and manually curated endospore-forming Firmicutes species. This collection may serve as a guide to conduct future experiments in endospore formers in genomic and metagenomic projects. DATA DESCRIPTION: Similar to the report of Davidson et al. (PLoS Genet 14:1-33, 2018), we used Pfam profiles (El-Gebali et al. in Nucleic Acids Res 47:D427-32, 2019) defining each protein and the genomic context surrounding the query gene to predict probable orthologs of the Spo0 pathway in Firmicutes. We present in this note a collection of 325 Firmicutes species organized by phylogenetic class and classified as spore formers, non-spore formers or unknown spore phenotype based on published literature, for which we predicted probable orthologs defining the signal transduction pathway initiating sporulation.

The unphosphorylated form of the PilR two-component system regulates pilA gene expression in Geobacter sulfurreducens.

In Geobacter sulfurreducens, metal reduction and generation of bioelectricity require the participation of several elements, and among them, the type IV pili has an essential role. The pilus is composed of multiple PilA monomers. Expression of pilA gene depends mainly on the σ54 factor and the response regulator protein PilR. In this work, we characterized the role of the PilS-PilR two-component system in the regulation of the pilA gene expression. Experimental evidence indicates that PilS is autophosphorylated at the His-334 residue, which in turn is transferred to the conserved Asp-53 in PilR. Contrary to other PilS-PilR systems, substitution D53N in PilR resulted in higher activation of the pilA gene. By using a pilA::luxCDABE fusion with different promoter fragments and in vitro DNA-binding assays, we demonstrated the existence of multiple functional PilR binding sites. A regulatory model in which the non-phosphorylated PilR protein directs activation of pilA expression by binding to two sites in the promoter region of this gene is presented.

Colistin asociado a bloqueo neuromuscular postanestésico prolongado. reporte de caso clínico / Colistin associated postanesthetic prolonged neuromuscular blockade: case report

En los últimos años se ha reconsiderado la utilización de antibióticos como el colistin en infecciones por microorganismos multiresistentes, esta situación ha ocasionado el resurgimiento de las reacciones adversas como la nefrotoxicidad y neurotoxicidad que provocaron su abandono en los 80s. Se presenta caso clínico de paciente femenina de 75 años que manifiesta toxicidad neurológica con bloqueo neuromuscular residual postanestésico asociado a tratamiento con colistin.

In recent years there has been reconsidered the use of antibiotics such as colistin in infections caused by multiresistant microorganisms, this situation has caused the resurgence of adverse reactions such as nephrotoxicity and neurotoxicity that led to its abandonment in the 80s. Herein, we present a case of a 75 years old woman with neurotoxicity associated with colistin treatment manifested by postanesthesia prolonged neuromuscular blockade.

Intubación guiada por fibroscopio flexible en una paciente con trauma raquimedular cervical, manejado por el servicio de anestesiología / Intubation guided by flexible fiberoptic in a patient with cervical spinal cord trauma, managed by the anesthesia service

La intubación con el uso de fibroscopía flexible en paciente despierto, actualmente es la técnica de elección para el manejo de pacientes con vía aérea difícil, lamentablemente no todos los anestesiólogos están entrenados para realizar ésta técnica, siendo deber de todo anestesiólogo el control de la permeabilidad de la vía aérea, esta técnica se convierte en el estándar de oro para el manejo de la intubación en pacientes con vía aérea dificil en anestesiología. El presente caso clínico se trata de una paciente con trauma raquimedular cervical, inmovilizada con Halo - Vest (halotórax) en la que se realizó intubación guiada por fibroscopía flexible con la paciente despierta, manejada por el servicio de anestesiología, procedimiento que se realizo de manera exitosa y que permitió la realización de la fijación quirúrgica de las fracturas y listesis cervicales.

Intubation using flexible fiberoptic in awake patient, now is the technique of choice for the management of patients with difficult airways, unfortunately not all anesthesiologists are trained to perform this technique, it is the duty of every anesthesiologist control permeability the airway, this technique becomes the gold standard for management of intubation in patients with difficult airway in anesthesiology. This case report is of a patient with cervical spinal cord trauma, immobilized with Halo - Vest (halotorax) which was guided fiberoptic intubation with the patient awake, managed by the anesthesiology service, procedure performed in and allowed successfully performing surgical fixation of fractures and cervical listesis.

Anestesia General balanceada con Flujos bajos / General Aenesthesia balanced with low flow

La anestesia general con flujos bajos, es para nuestro medio una práctica reciente, en cambio en otros países como Es paña y México esta técnica se practica desde la década pasada ya que requiere de modernas maquinas de anestesia, dotadas de circuitos circulares y sistemas de monitorización de gases, monitorización de la ventilación pulmonar, y monitores multiparamétricos de signos vitales (de 6 canales), que han permitido el desarrollo reciente de esta técnica en nuestro medio. El presente estudio tiene como propósito fundamental proponer una nueva técnica que hoy en día es una necesidad para dosificar mejor los anestésicos y proporcionar un mejor margen de seguridad, reducir el riesgo anestésico y por ende administrar una anestesia más segura, efectiva y eficiente a los pacientes, disminuyendo así, la morbilidad posoperatoria de la vía aérea porque proporciona gases húmedos y calientes. La reducción del flujo de gas fresco a 1 litro por minuto (02), nos permite ahorrar tanto 02 como el gas anestésico por lo tanto se reducirán los costos principalmente de los nuevos gases anestésicos cuyo valor es prohibitivo para nuestro medio y al mismo tiempo reduciremos la contaminación de los quirófanos y el medio ambiente.

The general anesthesia with low flow, is for us a recent practice but not in Spain and Mexico, this technique is practiced since ten years ago and require modern anesthesia machines that are equipped with a circle rebreathing system, monitoring of inspiratory and expiratory gas concentrations necessary This project has permitted propose new technique that today is a need to facilitates precise anesthetics administration for the patient. That reduce the anesthetics risks doing a efective, safe and eficient anesthesia but also makes gases more warm and humid reducing the postoperatory morbidity airway patient's. When Fresh Gas Flow is reduced to 1.0 L/min, reduce the costs of volatile anesthetics and provide considerable economic and ecological benefits, but may also improve the quality of patient care

Comparación de la eficacia del bloqueo neuromuscular con besilato de atracurio (genérico) y la molécula original (trccrium) / Comparison of the efficacy of the neuromuscular blockade with atracurium besilate (generic) and the original molecule (trccrium)

El presente trabajo de investigación tiene el objetivo de comparar la farmacodinámia tanto del Besilato de Atracurio Tracrium® (molécula original), con la del Besilato de Atracurio (Genérico), a través del monitoreo de la función neuromuscular con un estimulador neuromuscular periférico y de esta forma recoger la información necesaria para facilitar la toma de decisión en la elección del producto farmacéutico que reúna las mejores condiciones tanto de eficacia farmacológica como económica. Se realizó un estudio prospectivo en 35 pacientes ASA I, distribuidos aleatoriamente en dos grupos Grupo A (n=18) en los que se administró la droga genérica y el grupo B (n=17) en los que se administró la molécula original, con posterior monitoreo de la función neuromuscular con un neuroestimulador periférico. Concluyéndose que tanto el Besilato de Atracurio genérico como la molécula original Tracrium® tienen características farmacodinámicas muy similares y ambas son eficaces para producir un bloqueo neuromuscular adecuado, dándonos la posibilidad de poder decidir la mejor oferta económica sin el temor de adquirir una droga que no sea eficaz.

The present investigation have the objective to compare the pharmacodynamics characteristic of Besilatio of Atracurium (original molecule) and the Besilatio of Atracurium (Generic), trough the monitoring of the neuromuscular function with a neuromuscular peripheal stimulative; this will provide the enough information to make the rigth decision in the election o the farmaceutical product that gathers the best conditions among the pharmaceutical efectiveness and the economic part We made a prospective study with 35 patients ASA I, distributed in two groups: Group A (n=18), those to whom it was administraded the Generic drug, and Group B (n=17), those whom it was administraded the Original Molecule, with a later check through neuromuscular peripheal stimulous. We conduyed than the Besilatio of Atracurium like the original molecule have the same pharmacodynamics characteristic to produce an appropiate neuromuscular blockade, giving us the choice to choose the best economic ofert, without the fear of adquiring a drug that is not efective.