Uma reflexão sobre as conexões entrea fonoaudiologia e Saúde Única / A reflection on the connections betweenspeech therapy and One Health

A Saúde Única é uma crescente abordagem sistêmica para aumentar indissociavelmente os níveis de saúde das pessoas, dos animais e do ambiente no planeta. Por sua característica interdisciplinar e multidisciplinar, a Saúde Única tem arregimentado cada vez mais novos agentes na área de saúde, ampliando novas fronteiras de prática profissional. Objetivo: tendo em vista de que a abordagem sistêmica da Saúde Única (One Health) é holística, objetivou-se descrever e refletir sobre o papel da fonoaudiologia nesse contexto. Métodos: fez-se uma revisão de escopo sobre as ligações da fonoaudiologia com as desordens de saúde que são o foco em Saúde Única. Realizou-se uma procura de artigos nas plataformas PubMed e SciELO, com a combinação booleana dos indexadores "fonoaudiologia" e "Saúde Única", em idioma português; e as palavras "speech-language therapy" e "One Health" em idioma inglês. O operador booleano foi "E" e "AND". Adicionalmente, procurou-se teses e dissertações com esses mesmos operadores e combinações no Catálogo de Teses e Dissertações da CAPES. Analisou-se os dados por uma abordagem qualitativa, por isso os métodos estatísticos não foram aplicados. A partir dessa abordagem, complementou-se a argumentação com uma reflexão crítica sobre a inclusão da fonoaudiologia na estratégia de Saúde Única. Resultados: apenas na plataforma SciELO encontrou-se cinco obras com os descritores e a combinação "Saúde Única AND fonoaudiologia"; esse resultado é fortemente sugestivo de que o profissional em fonoaudiologia não tem sido incluído como um agente na abordagem Saúde Única. Contrariando esse cenário, argumenta--se que a fonoaudiologia pode preencher um nicho de trabalho e atuação acadêmica na abordagem de Saúde Única, cujo foco são desordens de saúde que podem resultar em distúrbios da comunicação nas pessoas. Considerações finais: há um nicho para a fonoaudiologia na abordagem da Saúde Única para a prevenção, o tratamento e a investigação científica das desordens da comunicação humana.

One Health is a growing systemic approach aimed at increasing the health levels of people, animals and the environment on the planet. Due to its inter- and multidisciplinary characteristics, One Health has been recruiting an increasing number of new agents in the health area, expanding new frontiers of professional practice. Objective: considering that the systemic approach of One Health is holistic, the objective is to describe and reflect on the role of speech therapy in this context. Methods: a scoping review was conducted to explore the connections between speech therapy and the health disorders that are the focus of One Health. A search for articles was carried out on the PubMed and SciELO platforms, using the Boolean combination of the terms "fonoaudiologia" (speech therapy) and "Saúde Única" (One Health), in Portuguese; and the words "speech-language therapy" and "One Health" in English using the Boolean operator "E" and "AND". Additionally, we searched for theses and dissertations with these same operators and combinations, in the CAPES Catalog of Theses and Dissertations. The data was analyzed using a qualitative approach, therefore statistical methods were not applied. Based on this approach, the argument was complemented with a critical reflection on the inclusion of speech therapy in the One Health strategy. Results: five works were found on the SciELO platform using the descriptors and combination "Saúde Única AND fonoaudiologia". This result suggests that the speech therapy professional has not been included as an agent in the One Health approach. Contrary to this scenario, it is argued that speech therapy can play a significant professional and academic role in One Health, which focuses on health disorders that may result in communication disorders. Final considerations: there is a niche for speech therapy in the One Health approach to the prevention, treatment and scientific investigation of human communication disorders.

Os desafios diagnósticos da cardiomiopatia hipertrófica / The diagnostic challenges of hypertrophic cardiomyopathy

INTRODUÇÃO: A Cardiomiopatia Hipertrófica (CMH) é a doença cardíaca genética mais comum, com prevalência em 1 a cada 500 pessoas. A principal característica é a hipertrofia ventricular esquerda na ausência de patologias que justifiquem tal alteração. A CMH pode apresentar desde formas assintomáticas até insuficiência cardíaca refratária e morte súbita cardíaca (MSC). Possui padrão de herança autossômica dominante com expressividade e penetrância variáveis. Para diagnóstico e estratificação do risco de MSC dispõe-se do Eletrocardiograma (ECG), Ecocardiograma (ECOTT), Holter 24h, Teste Ergométrico (TE), Ressonância Magnética (RM) e Testes Genéticos. RELATO DE CASO: A.O.A, 60 anos, natural de SP, dislipidêmico, ex- atleta profissional de corridas de curta distância, que se aposentou aos 40 anos. Encaminhado ao ambulatório de Cardiologia do Esporte do Instituto Dante Pazzanese de Cardiologia para avaliação. Praticante de corridas de rua 3x/semana, treinos de 10-12km. Negou sintomas, assim como histórico de doenças cardiovasculares na família. ECG: Ritmo sinusal, sinais de sobrecarga ventricular esquerda com alterações da repolarização ventricular difusas (ondas T's profundas). Exame físico sem alterações. Aventada hipótese diagnóstica de CMH e optado por suspender atividades físicas até realização dos exames. ECOTT evidenciou cavidades normais, espessura miocárdica preservada, função preservada, sem anormalidades valvares. RM fibrose ou hipertrofia miocárdica. TE mostrou boa capacidade funcional e ausência de arritmias esforço induzidas. Alterações eletrocardiográficas persistentes apesar do destreinamento. Mantido acompanhamento regular devido à possibilidade de manifestação de CMH. Após 6 anos, novo ECOTT evidenciou hipertrofia assimétrica, septo 12mm e parede posterior de 9mm. Realizada RM, a qual mostrou hipertrofia septal simétrica apical (14mm) com padrão de fibrose mesocárdica na porção apical da parede inferior. CONCLUSÃO: No presente relato, apesar do ECG muito sugestivo de CMH, demais exames não apresentaram alterações significativas. Todavia a doença possui expressividade e penetrância variáveis, podendo se manifestar ao longo da vida e as alterações eletrocardiográficas podem anteceder as alterações estruturais cardíacas. Por isso, é de extrema importância acompanhar esses pacientes e, assim, atuar na prevenção da MSC. No presente relato de caso as alterações eletrocardiográficas antecederam em 6 anos as alterações apresentadas na segunda RM.

Acurácia do testes ergométricos para avaliar isquemia em atletas / Accuracy of exercise testing to assess ischemia in athletes

INTRODUÇÃO: O teste ergométrico (TE) na investigação de doença arterial coronariana (DAC), demonstra, na literatura, sensibilidade entre 61% e 73% e especificidade entre 69% e 81%. O valor preditivo positivo do TE se mostra maior em homens, em todas as faixas etárias, devido à alta prevalência de DAC. As mulheres, em geral, apresentam menor prevalência de DAC e, portanto, maior presença de testes falso-positivos em relação aos homens. O TE é indicação classe I B na diretriz Brasileira de cardiologia do Exercício e do Esporte para atletas profissionais ≥ 60 anos e classe IIa B para esportistas de 35-59 anos que praticam atividade física de alta intensidade e competição esportiva. OBJETIVO: Avaliar a acurácia de TE para isquemia em atletas de uma instituição de referência em cardiologia do esporte do estado de São Paulo. MÉTODOS: Estudo observacional, retrospectivo e unicêntrico, com seleção de pacientes atletas atendidos em um ambulatório de cardiologia esportiva de abril de 2023 a abril de 2024. RESULTADOS: Foram incluídos 218 pacientes que realizaram TE, onde 66 apresentaram isquemia associado a alteração do segmento ST (sensibilidade 30%), sendo submetidos a cintilografia de perfusão miocárdica (CPM) e/ou angiotomografia das artérias coronárias para seguimento de investigação. Dos 66 pacientes, 14 apresentaram angiotomografias alteradas e 2 com CPM sugestivas de isquemia, totalizando 24% com resultados positivos para doença aterosclerótica. A média de idade da população geral foi de 59 anos, da população feminina de 58 anos (variação de 32 a 77 anos), da masculina 61 anos (variação de 39 a 81 anos). Do total de paciente, aproximadamente 25% eram do sexo feminino (55 pacientes), com 18 TE com alteração isquêmica (32%), sendo que, desses pacientes, nenhum apresentava CPM alterada e 2 com angiotomografia alteradas, totalizando 1% verdadeiro positivo. 163 pacientes do sexo masculino, com 49 TE com isquemia (30%), sendo 2 CPM e 13 angiotomografias alteradas, totalizando 30% verdadeiros positivos. Média de equivalente metabólico da população geral foi de 15,5 MET's, da população masculina com TE alterado foi de 15,8 MET's, e da feminina 15,2 MET's. CONCLUSÃO: O presente trabalho demonstra a limitação do TE para diagnóstico de DAC, principalmente em atletas que possuem uma boa capacidade funcional. Já em relação ao gênero feminino, a capacidade do teste ergométrico em diagnosticar DAC é ainda menor, com 99% de resultados falsos positivos nesta presente análise

Lipid Nanoparticles Elicit Reactogenicity and Sickness Behavior in Mice Via Toll-Like Receptor 4 and Myeloid Differentiation Protein 88 Axis.

mRNA therapeutics encapsulated in lipid nanoparticles (LNPs) offer promising avenues for treating various diseases. While mRNA vaccines anticipate immunogenicity, the associated reactogenicity of mRNA-loaded LNPs poses significant challenges, especially in protein replacement therapies requiring multiple administrations, leading to adverse effects and suboptimal therapeutic outcomes. Historically, research has primarily focused on the reactogenicity of mRNA cargo, leaving the role of LNPs understudied in this context. Adjuvanticity and pro-inflammatory characteristics of LNPs, originating at least in part from ionizable lipids, may induce inflammation, activate toll-like receptors (TLRs), and impact mRNA translation. Knowledge gaps remain in understanding LNP-induced TLR activation and its impact on induction of animal sickness behavior. We hypothesized that ionizable lipids in LNPs, structurally resembling lipid A from lipopolysaccharide, could activate TLR4 signaling via MyD88 and TRIF adaptors, thereby propagating LNP-associated reactogenicity. Our comprehensive investigation utilizing gene ablation studies and pharmacological receptor manipulation proves that TLR4 activation by LNPs triggers distinct physiologically meaningful responses in mice. We show that TLR4 and MyD88 are essential for reactogenic signal initiation, pro-inflammatory gene expression, and physiological outcomes like food intake and body weight─robust metrics of sickness behavior in mice. The application of the TLR4 inhibitor TAK-242 effectively reduces the reactogenicity associated with LNPs by mitigating TLR4-driven inflammatory responses. Our findings elucidate the critical role of the TLR4-MyD88 axis in LNP-induced reactogenicity, providing a mechanistic framework for developing safer mRNA therapeutics and offering a strategy to mitigate adverse effects through targeted inhibition of this pathway.

Real-world effectiveness of original BNT162b2 mRNA COVID-19 against symptomatic Omicron infection among children 5-11 years of age in Brazil: A prospective test-negative design study.

OBJECTIVE: To estimate original wild-type BNT162b2 effectiveness against symptomatic Omicron infection among children 5-11 years of age. METHODS: This prospective test-negative, case-control study was conducted in Toledo, southern Brazil, from June 2022 to July 2023. Patients were included if they were aged 5-11 years, sought care for acute respiratory symptoms in the public health system, and were tested for SARS-CoV-2 using reverse transcription polymerase chain reaction. In the primary analysis, we determined the effectiveness of two doses of original wild-type BNT162b2 against symptomatic COVID-19. The reference exposure group was the unvaccinated. RESULTS: A total of 757 children were enrolled; of these, 461 (25 cases; 436 controls) were included in the primary analysis. Mean age was 7.4 years, 49.7 % were female, 34.6 % were obese, and 14.1 % had chronic pulmonary disease. Omicron accounted for 100 % of all identified SARS-CoV-2 variants with BA.5, BQ.1, and XBB.1 accounting for 35.7 %, 21.4 % and 21.4 %, respectively. The adjusted estimate of two-dose vaccine effectiveness against symptomatic Omicron was 3.1 % (95 % CI, -133.7 % to 61.8 %) after a median time between the second dose and the beginning of COVID-19 symptoms of 192.5 days (interquartile range, 99 to 242 days). CONCLUSION: In this study with children 5-11 years of age, a two dose-schedule of original wild-type BNT162b2 was not associated with a significant protection against symptomatic Omicron infection after a median time between the second dose and the beginning of COVID-19 symptoms of 192 days, although the study may have been underpowered to detect a clinically important difference. TRIAL REGISTRATION NUMBER: ClinicalTrials.gov number, NCT05403307 (https://classic. CLINICALTRIALS: gov/ct2/show/NCT05403307).

Mutations in glioblastoma proteins do not disrupt epitope presentation and recognition, maintaining a specific CD8 T cell immune response potential.

Antigen-specific cytotoxic CD8 T cells are extremely effective in controlling tumor growth and have been the focus of immunotherapy approaches. We leverage in silico tools to investigate whether the occurrence of mutations in proteins previously described as immunogenic and highly expressed by glioblastoma multiforme (GBM), such as Epidermal Growth Factor Receptor (EGFR), Isocitrate Dehydrogenase 1 (IDH1), Phosphatase and Tensin homolog (PTEN) and Tumor Protein 53 (TP53), may be contributing to the differential presentation of immunogenic epitopes. We recovered Class I MHC binding information from wild-type and mutated proteins using the Immune Epitope Database (IEDB). After that, we built peptide-MHC (pMHC-I) models in HLA-arena, followed by hierarchical clustering analysis based on electrostatic surface features from each complex. We identified point mutations that are determinants for the presentation of a set of peptides from TP53 protein. We point to structural features in the pMHC-I complexes of wild-type and mutated peptides, which may play a role in the recognition of CD8 T cells. To further explore these features, we performed 100 ns molecular dynamics simulations for the peptide pairs (wt/mut) selected. In pursuit of novel therapeutic targets for GBM treatment, we selected peptides where our predictive results indicated that mutations would not disrupt epitope presentation, thereby maintaining a specific CD8 T cell immune response. These peptides hold potential for future GBM interventions, including peptide-based or mRNA vaccine development applications.

Nutritive tissue rich in reserves in the cell wall and protoplast: the case of Manihot esculenta (Euphorbiaceae) galls induced by Iatrophobia brasiliensis (Diptera, Cecidomyiidae).

The galls can offer shelter, protection, and an adequate diet for the gall-inducing organisms. Herein, we evaluated the structure of Manihot esculenta leaves and galls induced by Iatrophobia brasiliensis in order to identify metabolic and cell wall composition changes. We expected to find a complex gall with high primary metabolism in a typical nutritive tissue. Non-galled leaves and galls were subjected to anatomical, histochemical, and immunocytochemical analyses to evaluate the structural features, primary and secondary metabolites, and glycoproteins, pectins, and hemicelluloses in the cell wall. The gall is cylindric, with a uniseriate epidermis, a larval chamber, and a parenchymatic cortex divided into outer and inner compartments. The outer compartment has large cells with intercellular spaces and stocks starch and is designated as storage tissue. Reducing sugars, proteins, phenolic compounds, and alkaloids were detected in the protoplast of inner tissue cells of galls, named nutritive tissue, which presents five layers of compact small cells. Cell walls with esterified homogalacturonans (HGs) occurred in some cells of the galls indicating the continuous biosynthesis of HGs. For both non-galled leaves and galls, galactans and xyloglucans were broadly labeled on the cell walls, indicating a cell growth capacity and cell wall stiffness, respectively. The cell wall of the nutritive tissue had wide labeling for glycoproteins, HGs, heteroxylans, and xyloglucans, which can be used as source for the diet of the galling insect. Manihot esculenta galls have compartments specialized in the protection and feeding of the galling insect, structured by nutritive tissue rich in resource compounds, in the cell walls and protoplast.

Repercussões da Covid-19 na percepção de mudanças de peso e satisfação corporal: comparação entre mulheres com e sem transtornos alimentares / Impacts of Covid-19 on weight change perception and body satisfaction: a comparison between women with and without eating disorders

Introdução: A pandemia da Covid-19 trouxe consequências diversas para a saúde mental e física das pessoas. O contexto de isolamento social potencializou problemas relacionados à imagem corporal, principalmente em pessoas com transtornos alimentares. Objetivo: Analisar as repercussões da Covid-19 em variáveis antropométricas em relação a percepção de peso e satisfação corporal, comparando mulheres com transtornos alimentares e aquelas sem essa condição. Métodos: Estudo observacional, descritivo, comparativo e transversal. Mulheres com transtornos alimentares em tratamento (GTA) foram recrutadas de cinco serviços nas regiões Sul e Sudeste. A contrapartida, o grupo controle (GCO) foi composto por mulheres sem TA, recrutadas on-line e com o escore negativo no teste EAT-26. Questionários on-line foram aplicados entre junho de 2020 e janeiro de 2021. Análises estatísticas incluíram Qui-quadrado de Pearson ou Exato de Fisher e regressões logísticas pelo SPSS 23.0. Resultados: Participaram 174 mulheres jovens (31,94±9,35 anos), com formação superior (71,3%) e companheiro (61,5%), que estavam em isolamento social. O GTA foi composto por 58 mulheres e GCO, 116. A maioria (55,2%) do GTA notou aumento do peso corporal, enquanto se mostrou pouco satisfeita com a imagem corporal (81%), resultado diferente estatisticamente do GCO. A associação entre mulheres com TA e pouca satisfação corporal foi significativa (p=0,0010). Aquelas em isolamento social e com sobrepeso ou obesidade tiveram maior probabilidade de percepção de aumento de peso. Conclusão: A pandemia da Covid-19 intensificou a insatisfação corporal, principalmente entre mulheres com transtornos alimentares. Aquelas em isolamento com sobrepeso ou obesidade perceberam mais aumento de peso, apontando a necessidade de estratégias de intervenção para esses grupos.

Introduction: The Covid-19 pandemic led to various consequences for people's mental and physical health. The context of social isolation exacerbated problems related to body image, especially in individuals with eating disorders. Objective: To analyze the repercussions of Covid-19 on anthropometric variables regarding weight perception and body satisfaction, comparing women with eating disorders to those without this condition. Methods: Observational, descriptive, comparative, and cross-sectional study. Women with eating disorders in treatment (EDG) were recruited from five services in the South and Southeast regions of Brazil. The counterpart, the control group (CG), consisted of women without eating disorders recruited online and with a negative score on the EAT-26 test. Online questionnaires were applied between June 2020 and January 2021. Statistical analyses included Pearson's Chi-square or Fisher's Exact test and logistic regressions, performed using the SPSS 23.0 program. Results: Participants were 174 young women (31.94±9.35 years of age), with higher education (71.3%) and with a partner (61.5%), who were in social isolation. The EDG consisted of 58 women, with116 in the CG. The majority (55.2%) of the EDG noticed an increase in body weight while being dissatisfied with body image (81.0%), a statistically different result from the CG. The association between women with eating disorders and low body satisfaction was significant (p=.0010). Those in social isolation and overweight or obese were more likely to perceive weight gain. Conclusion: The Covid-19 pandemic intensified body dissatisfaction, especially among women with eating disorders. Those in isolation who were overweight or obese perceived more weight gain, indicating the need for intervention strategies for these groups.

Epidemiological Profile of Ophir Loyola Cancer Hospital: A Snapshot of the Incidence of Solid Neoplasms in the Eastern Amazon.

BACKGROUND: Solid neoplasms have a heterogeneous incidence worldwide and in Brazil. Thus, the region delimited by the Legal Amazon has a distinct epidemiological profile. In Pará, Ophir Loyola Cancer Hospital(OLCH) accounts for 71.11% of hospital visits in the state. METHODS: This was an ecological, exploratory, and mixed descriptive studythat investigated the epidemiological profile of patients with cancer treated at OLCH from January to December 2020. Sociodemographic data at admission were the primary variables, which were analyzed according to spatial distribution. RESULTS: In this study, the data of 2952 patients were analyzed, with the majority being between the ages of 50 and 79 years (62.47%), female (59.49%), and diagnosed but without previous treatment (87.30%). The most common cancers were breast (16.50%), cervical (13.40%), stomach (8.98%), and prostate (7.72%). Of the 12 integration regions, Guajará had the highest number of referrals (49.86%), followed by Guamá (12.94%) and Caeté River (8.98%). CONCLUSION: The profile of care at OLCH showed a high incidence of solid malignancies compared to that in other regions of Brazil, indicating environmental and sociocultural influences on the carcinogenic profile present in the eastern Amazon.

Outstanding adsorption capacity of iron oxide synthesized with extract of açaí berry residue: kinetic, isotherm, and thermodynamic study for dye removal.

Contamination of water by toxic dyes is a serious environmental problem. Adsorbents prepared by an environmentally safe route have stood out for application in pollutant removal. Herein, iron oxide-based nanomaterial composed of Fe(III)-OOH and Fe(II/III) bound to proanthocyanidins, with particles in the order of 20 nm, was prepared by green synthesis assisted by extract of açaí (Euterpe oleracea Mart.) berry seeds from an agro-industrial residue. The nanomaterial was applied in the adsorption of cationic dyes. Screening tests were carried out for methylene blue (MB), resulting in an outstanding maximum adsorption capacity of 531.8 mg g-1 at 343 K, pH 10, 180 min. The kinetics followed a pseudo-second-order model and the isotherm of Fritz-Schülnder provided the best fit. Thermodynamic data show an endothermic process with entropy increase, typical of chemisorption. The proposed mechanism is based on the multilayer formation over a heterogeneous adsorbent surface, with chemical and electrostatic interactions of MB with the iron oxide nanoparticles and with the proanthocyanidins. The high adsorption efficiency was attributed to the network formed by the polymeric proanthocyanidins that entangled and protected the iron oxide nanoparticles, which allowed the reuse of the nanomaterial for seven cycles without loss of adsorption efficiency.

Exploring the Immune Response against RSV and SARS-CoV-2 Infection in Children.

Viral respiratory tract infections are a significant public health concern, particularly in children. RSV is a prominent cause of lower respiratory tract infections among infants, whereas SARS-CoV-2 has caused a global pandemic with lower overall severity in children than in adults. In this review, we aimed to compare the innate and adaptive immune responses induced by RSV and SARS-CoV-2 to better understand differences in the pathogenesis of infection. Some studies have demonstrated that children present a more robust immune response against SARS-CoV-2 than adults; however, this response is dissimilar to that of RSV. Each virus has a distinctive mechanism to escape the immune response. Understanding the mechanisms underlying these differences is crucial for developing effective treatments and improving the management of pediatric respiratory infections.

Evidence-based pharmacological prophylaxis recommendations for venous thromboembolism in hospitalized acutely ill medical patients: a systematic review of clinical practice guidelines.

Venous thromboembolism is a complex multifactorial disease considered the most common cause of preventable deaths in hospitalized patients. Recommendations about pharmacological venous thromboembolism prophylaxis in adult hospitalized patients are available in clinical practice guidelines for optimization of healthcare delivery and improvement of patient outcomes. We conducted a systematic review of clinical practice guidelines using ADAPTE to synthesize recommendations for pharmacological prophylaxis of venous thromboembolism in hospitalized medical patients at a medium complexity university hospital. Recommendations for pharmacological prophylaxis were extracted from seven clinical practice guidelines considered of high quality after assessment with the Appraisal of Guidelines for Research and Evaluation (AGREE II) instrument. These recommendations will support discussion with specialists and implementation of practices in the setting of the hospital studied.

O tromboembolismo venoso é uma doença multifatorial complexa, considerada uma causa comum de óbitos evitáveis em pacientes hospitalizados. Recomendações sobre profilaxia farmacológica de tromboembolismo venoso em pacientes adultos hospitalizados estão disponíveis em diretrizes clínicas para otimizar os cuidados à saúde e contribuir com a melhora do desfecho do paciente. Dessa forma, foi conduzida uma revisão sistemática de diretrizes clínicas utilizando a metodologia ADAPTE para sintetizar as recomendações para profilaxia farmacológica de tromboembolismo venoso em pacientes clínicos adultos hospitalizados em um hospital universitário de média complexidade. As recomendações para profilaxia farmacológica foram extraídas de sete diretrizes clínicas consideradas de alta qualidade após avaliação pelo Appraisal of Guidelines for Research and Evaluation (AGREE II). Essas recomendações servirão de apoio para discussão com especialistas e implementação de práticas dentro do contexto do hospital estudado.

Constituição psíquica e linguística de um sujeito com transtorno do espectro do autismo imerso em uma família vulnerável pela imigração no pós-guerra / Psychic and linguistic constitution of a subject with autism spectrum disorder immersed in a family vulnerable by post-war immigration / Constitución psíquica y lingüística de un sujeto con trastorno del espectro autismo inmerso en una familia vulnerable por inmigración de posguerra

Introdução: este artigo apresenta a constituição psíquica e linguística de um jovem autista, proveniente de uma família de imigrantes em situação de pós-guerra, em que entram em questão temas como o luto, a constituição psíquica transgeracional, e a presença de angústias no processo de desenvolvimento da criança em uma situação singular que é a presença do autismo. Objetivo: analisar os efeitos singulares da imigração e multiculturalismo em um caso de autismo e sua evolução terapêutica. Método: estudo de caso longitudinal, que utilizou diário clínico e filmagens de sessões com observações do desenvolvimento de Rafael, desde os dezoito meses até a idade adulta. Como abordagem terapêutica e análise dos resultados, foram utilizados aportes da constituição psíquica da teoria psicanalítica, e sobre o desenvolvimento linguístico em uma perspectiva enunciativa. Resultados: O multiculturalismo acarretava um desafio maior ao processo de aquisição da linguagem por parte da criança com autismo, enquanto o silêncio consequente da dor do luto, presente nos adultos, dificultava a troca verbal e atrasava sua constituição psíquica. O autismo, por sua vez, apresentou-se como transtornos qualitativos na comunicação, necessitando maior investimento por parte de seus cuidadores para que a aquisição da linguagem se desse, pois o paciente precisou ser fisgado para a nossa cultura. Conclusão: Diante de todo esse quadro, o caso clínico demonstra a importância do suporte terapêutico à família e do investimento contínuo na subjetivação, considerando e valorizando os diferentes códigos culturais que compõem o núcleo familiar. (AU)

Introduction: this article presents the psychic and linguistic constitution of an autistic young man, from a post-war immigrant family, in which themes such as mourning, the transgenerational psychic constitution, and the presence of anxieties in the process come into question of the child development in a unique situation that is the presence of autism. Objective: to analyze the unique effects of immigration and multiculturalism in a case of autism and its therapeutic evolution. Method: longitudinal case study, which used a clinical diary and footage of sessions with observations of the development of R. from eighteen months to adulthood. As a therapeutic approach and analysis of results, contributions from the psychic constitution of psychoanalytic theory, and on linguistic development in an enunciative perspective, were used. Results: Multiculturalism posed a greater challenge to the process of language acquisition by the child with autism, while the consequent silence of the pain of grief, present in adults, hindered verbal exchange and delayed their psychic constitution. Autism, in turn, presented itself as qualitative disorders in communication, requiring greater investment on the part of its caregivers for the acquisition of language to take place, as it needed to be hooked for our culture. Conclusion: Given this situation, this clinical case demonstrates the importance of therapeutic support to the family and the continuous investment in subjectivity, considering and valuing the different cultural codes that make up the family nucleus. (AU)

Introducción: este artículo presenta la constitución psíquica y lingüística de un joven autista, proveniente de una familia inmigrante de posguerra, en la que se cuestionan temas como el luto, la constitución psíquica transgeneracional y la presencia de ansiedades en el proceso del desarrollo del niño en una situación única que es la presencia del autismo. Objetivo: analizar los efectos singulares de la inmigración y la multiculturalidad en un caso de autismo y su evolución terapéutica. Método: estudio de caso longitudinal, que utilizó un diario clínico y metraje de sesiones con observaciones del desarrollo de R. desde los dieciocho meses hasta la edad adulta. Como abordaje terapéutico y análisis de resultados se utilizaron aportes desde la constitución psíquica de la teoría psicoanalítica y sobre el desarrollo lingüístico en perspectiva enunciativa. Resultados: El multiculturalismo supuso un mayor desafío al proceso de adquisición del lenguaje por parte del niño con autismo, mientras que el consiguiente silencio del dolor del duelo, presente en los adultos, dificultó el intercambio verbal y retrasó su constitución psíquica. El autismo, a su vez, se presentó como un trastorno cualitativo en la comunicación, requiriendo una mayor inversión por parte de sus cuidadores para que se produjera la adquisición del lenguaje, pues necesitaba engancharse a nuestra cultura. Conclusión: Ante esta situación, este caso clínico demuestra la importancia del apoyo terapéutico a la familia y la continua inversión en la subjetividad, considerando y valorando los diferentes códigos culturales que conforman el núcleo familiar. (AU)

BNT162b2 mRNA COVID-19 against symptomatic Omicron infection following a mass vaccination campaign in southern Brazil: A prospective test-negative design study.

BACKGROUND: Evidence regarding effectiveness of BNT162b2 mRNA COVID-19 vaccine against Omicron in Latin America is limited. We estimated BNT162b2 effectiveness against symptomatic COVID-19 in Brazil when Omicron was predominant. METHODS: This prospective test-negative, case-control study was conducted in Toledo, Brazil, following a mass COVID-19 vaccination with BNT162b2. Patients were included if they were aged ≥12 years, sought care for acute respiratory symptoms in the public health system between November 3, 2021 and June 20, 2022, and were tested for SARS-CoV-2 using RT-PCR. In the primary analysis, we determined the effectiveness of two doses of BNT162b2 against symptomatic COVID-19. RESULTS: A total of 4,574 were enrolled; of these, 1,758 patients (586 cases and 1,172 controls) were included in the primary analysis. Mean age was 27.7 years, 53.8 % were women, and 90.1 % had a Charlson comorbidity index of zero. Omicron accounted for >97 % of all identified SARS-CoV-2 variants, with BA.1 and BA.2 accounting for 84.3 % and 12.6 %, respectively. Overall adjusted estimate of two-dose vaccine effectiveness against symptomatic COVID-19 was 46.7 % (95 %CI, 19.9 %-64.6 %) after a median time between the second dose and the beginning of COVID-19 symptoms of 94 days (IQR, 60-139 days). Effectiveness waned from 77.7 % at 7-29 days after receipt of a second dose to <30 % (non-significant) after ≥120 days. CONCLUSION: In a relatively young and healthy Brazilian population, two doses of BNT162b2 provided protection against symptomatic Omicron infection. However, this protection waned significantly over time, underscoring the need for boosting with variant-adapted vaccines in this population prior to waves of disease activity. TRIAL REGISTRATION NUMBER: ClinicalTrials.gov number, NCT05052307 (https://clinicaltrials.gov/ct2/show/NCT05052307).

Molecular evolution of dengue virus: a Bayesian approach using 1581 whole-genome sequences from January 1944 to July 2022.

Dengue is a viral disease transmitted by mosquitoes that has spread rapidly across all continents in recent years. There are four distinct but closely related serotypes of the virus that causes dengue (DENV-1, DENV-2, DENV-3, and DENV-4). In the present study, we evaluated temporal spreading and molecular evolution of dengue virus (DENV) serotypes. Bayesian coalescent analysis was performed to study viral evolution, and it was estimated that the most recent common ancestor of DENV-1 was present in 1884 in Southeast Asia, that of DENV-2 was present in 1723 in Europe, that of DENV-3 was present in 1921 in Southeast Asia, and that of DENV-4 was present in 1876 in Southeast Asia. DENV appears to have originated in Spain in approximately 1682, and it was disseminated in Asia and Oceania in approximately 1847. After this period, the virus was introduced into North America in approximately 1890. In South America, it was first disseminated to Ecuador in approximately 1897 and then to Brazil in approximately 1910. Dengue has had a significant impact on global health worldwide, and the present study provides an overview of the molecular evolution of DENV serotypes.

Relationship between enunciative signs of language acquisition and language assessment through the Bayley III scale at 24 months. / Relação entre sinais enunciativos de aquisição da linguagem e a avaliação de linguagem pela escala Bayley III aos 24 meses.

PURPOSE: To analyze the correlation between the results obtained on the SEAL and the Bayley III Scale and compare babies with and without delay in language acquisition at 24 months concerning the performance obtained by them and their mothers on the SEAL from 3 to 24 months. METHODS: The SEAL collection consists of 15-minute footages of 45 babies aged from 3 to 24 months old in interaction with their mothers, who were assessed by two trained speech therapists for the use of the SEAL. At 24 months, the 45 babies were assessed using the Bayley III Scale and the item language was selected to classify them with and without delay. These results were statistically analyzed through a Pearson's correlation test and a Fisher's exact test. RESULTS: In average, eighteen signs of typical development as we obtained, while a mean of 12 delay signs were found. By comparing the presence and absence of signs between the groups with and without delay in language acquisition, eight signs from the baby and one from the mother differed statistically in the sample. The analysis using the SEAL for cases of delay showed that the maternal factor was as important as the infant factor to understand the babies' language functioning. CONCLUSION: There was a significant correlation between the SEAL performance from 3 to 24 months and the language outcome at 24 months assessed by the Bayley III Scale in this sample.

OBJETIVO: Analisar a correlação entre resultados obtidos no Sinais Enunciativos de Aquisição da Linguagem (SEAL) e na Escala Bayley III e comparar bebês com e sem atraso na aquisição da linguagem aos 24 meses no desempenho obtido por ele e sua mãe no SEAL dos 3 aos 24 meses. MÉTODO: A coleta do SEAL constou de filmagens de 45 bebês, realizadas nas faixas etárias de 3 a 24 meses em interação com suas mães, com duração de 15 minutos, que foram avaliados por duas fonoaudiólogas treinadas no uso do SEAL. Aos 24 meses, os 45 bebês foram avaliados pela Escala Bayley III e selecionado o item linguagem para classificá-los com e sem atraso. Sobre tais resultados realizaram-se as análises estatísticas com o teste de correlação de Pearson e o teste exato de Fisher. RESULTADOS: Obtiveram-se as médias de sinais no desenvolvimento típico que foi 18 sinais e, em casos de atraso, a média foi de 12 sinais. Na comparação da relação de presença e ausência dos sinais entre os grupos com e sem atraso na aquisição da linguagem, oito sinais do bebê e um da mãe diferiram estatisticamente na amostra. O fator materno apresentou-se tão importante quanto o infantil na compreensão do funcionamento de linguagem dos bebês na análise realizada com o SEAL nos casos de atraso. CONCLUSÃO: Houve correlação significativa entre o desempenho no SEAL entre 3 e 24 meses e o desfecho de linguagem aos 24 meses avaliado pela Escala Bayley III nesta amostra.

Quantas infâncias? um estudo exploratório sobre discursos institucionais em defesa da primeira infância / How many childhoods? an exploratory study on institutional discourses in defense of early childhood

A defesa da Primeira Infância tem despontado no Brasil nos últimos anos com importante mobi-lização e militância dentro de um conjunto de lutas por direitos da infância e juventude mais amplo e que têm como matriz os Direitos Humanos e as conquistas de movimentos sociais que permitiram o advento do Estatuto da Criança e do Adolescente (ECA). Neste artigo, com o ob-jetivo de analisar especificidades do movimento pela Primeira Infância, apresentamos uma pesquisa documental exploratória sobre discursos de instituições e organizaçõesligadas a essa frente por meio de materiais veiculados em seus sítios eletrônicos. O material selecionado foi submetido à análise de conteúdo, meio pelo qual sistematizamos três categorias temáticas. Os resultados sugerem que parte das reivindicações dos grupos pela Primeira Infância concorrem com a Proteção Integral quanto à concepção do sujeito criança e adolescente e de seu desen-volvimento. Os achados foram discutidos criticamente e pela perspectiva da Psicologia Jurídica. (AU)

Early childhood has emerged in Brazil in recent years with important mobilization and mili-tancy within a set of struggles for the rights of the child embedded on Human Rights and the social movements that allowed the Brazil’s Child and Adolescent Statute to surge. In this arti-cle, and aiming to analyze Early Childhood movement specificities, we present an exploratory research on institutional and organizational discourses from Brazil’s Early Childhood move-ment on websites on the Internet. The collected data were submitted to content analysis, in a way that three analytical categories were created. The results suggest that some of those groups demands conflict with Full Protection advocacy regarding the very idea about children and adolescents and their development. The findings were discussed critically and from the perspective of Forensic Psychology. (AU)

SARS-CoV-2 immune complex triggers human monocyte necroptosis.

We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 and MLKL) were involved in SARS-CoV-2N1 gene expression in monocytes. SARS-CoV-2 immune complexes promoted monocyte necroptosis in a RIPK3- and MLKL-dependent manner, and Syk tyrosine kinase was necessary for SARS-CoV-2 immune complex-induced monocyte necroptosis, indicating the involvement of Fcγ receptors on necroptosis. Finally, we provide evidence that elevated LDH levels as a marker of lytic cell death are associated with COVID-19 pathogenesis.

Effect of the dietary inflammatory potential on the trajectory of body adiposity in a Brazilian cohort of university students.

OBJECTIVES: University students are vulnerable to unhealthy eating habits that characterize a proinflammatory diet. This study aimed to estimate the dietary inflammatory index (DII) and its association with the trajectory of body adiposity markers in university students. METHODS: The study analyzed data from 685 students entering a Brazilian public university in 2016 and 2017 and followed until 2018. DII was estimated from 39 dietary parameters obtained by 24-h dietary recall. Body adiposity was assessed by anthropometric markers and the percentage of body fat. Linear mixed-effects models were used to estimate the trajectory of adiposity markers according to DII tertiles. RESULTS: After adjustment for confounding variables, at baseline, DII showed a positive association with increased percentage of body fat among men (ß = 0.52; 95% CI: 0.01; 1.03) and waist-to-height ratio (WHtR; ß = 0.15; 95% CI: 0.12; 0.18) and among women with all body adiposity markers: BMI (ß = 0.68; 95% CI: 0.30; 1.05), percentage of body fat (ß = 1.43; 95% CI: 0.74; 2.11), WC (ß = 1.15; 95% CI: 0.41; 1.89) and WHtR (ß = 0.13; 95% CI:0,10; 0.16). The rate of change of the outcome variables over time was not associated with DII at baseline. CONCLUSIONS: The diet of university students in this Brazilian cohort study was characterized as proinflammatory and it was associated with body adiposity markers.