ABSTRACT
Schistosomiasis - caused by trematodes from the genus Schistosoma - affects more than 200 million people worldwide. Growing resistance to therapy with praziquantel (PZQ) has encouraged the search for novel treatments against this neglected disease. The compound 7-epiclusianone (7-epi) - isolated from 'bacupari' (the fruit of the Gracinia brasiliensis tree) - has promising activity against Schistosoma mansoni in vitro, damaging the parasite's tegument. However, the target and mechanism of action of 7-epi have not been identified. Here, we examined the possibility that 7-epi harms the tegument by inhibiting parasite superoxide dismutase (SOD), which protects the tegument from damage by reactive oxygen species produced by host immune cells. Molecular docking analysis in silico suggested strong interactions between 7-epi and S. mansoni cytosolic superoxide dismutase (SmCtSOD) at allosteric cavities. Schistosoma mansoni couples were cultivated ex vivo with 12.44-198.96 µm 7-epi for 24 h, and then parasite extracts were tested for lipid peroxidation (as a surrogate for oxidative stress), and SOD activity and expression. Lipid peroxidation levels increased after incubation with concentrations ≥99.48 µm 7-epi, and this compound reduced SOD activity at concentrations ≥24.87 µm. However, contact with 7-epi did not alter SOD expression, by quantitative real-time polymerase chain reaction (qRT-PCR). Our results show that the inhibition of SmCtSOD is partly responsible for the tegument detachment observed after incubation with 7-epi, but is not the only cause of the antiparasitic action of this compound in vitro.
Subject(s)
Benzophenones/pharmacology , Benzoquinones/pharmacology , Enzyme Inhibitors/pharmacology , Schistosoma mansoni/enzymology , Superoxide Dismutase/antagonists & inhibitors , Animals , Benzophenones/chemistry , Benzoquinones/chemistry , Enzyme Inhibitors/chemistry , Gene Expression Profiling , Integumentary System , Lipid Peroxidation , Molecular Docking Simulation , Real-Time Polymerase Chain Reaction , Schistosoma mansoni/drug effects , Superoxide Dismutase/biosynthesis , Superoxide Dismutase/chemistryABSTRACT
This research aimed to evaluate the interactions and effects of 2 and 4% addition levels of poultry slaughterhouse fat (chicken tallow) and soybean oil in diets for broiler chickens. Two experiments were carried out using one-day-old male Cobb chicks in an entirely random design with a 2x2 factorial scheme. In the first experiment, 560 chicks were used to evaluate performance and carcass characteristics. In the second experiment, 100 chicks were used to determine the nutrient digestibility, dietary energy utilization and the lipase and amylase pancreatic activity. There was no interaction between the fat sources and the addition levels for any of the analyzed variables, except for the digestibility coefficient of dry matter (DCDM), which was higher in diets added with 2% soybean oil when compared to chicken tallow. The addition of 4% fat in the diet, regardless of fat source, improved the digestibility coefficient of ethereal extract (DCEE) and increased weight gain and feed intake. Moreover, in the initial phase, the addition of 4% fat to the diet increased lipase activity when compared to diets with 2% addition, and a positive correlation between DCEE and pancreatic lipase activity was observed. In conclusion, there is no interaction between fat sources and addition levels, except for DCDM. Carcass characteristics are not influenced by any of the studied factors. The addition of 4% fat increases pancreatic lipase activity and improves DCEE, resulting in greater weight gain, regardless of the tested fat source, making chicken tallow a great alternative to soybean oil...
O objetivo deste trabalho foi avaliar as interações e os efeitos da utilização de gordura de abatedouro avícola (gordura de frango) e de óleo de soja com níveis de inclusão de 2 e 4% em dietas para frangos de corte. Foram conduzidos dois experimentos com pintos de corte de um dia de idade, machos, da linhagem Cobb, alojados num delineamento inteiramente ao acaso em esquema fatorial 2x2. No primeiro experimento utilizaram-se 560 aves para avaliar o desempenho e as características de carcaça. No segundo experimento foram alojadas 100 aves para determinar o aproveitamento dos nutrientes e da energia da dieta, e também a atividade de lipase e amilase pancreática. Não houve interações entre as fontes lipídicas e os níveis de inclusão para nenhuma das variáveis analisadas, com exceção do coeficiente de metabolizabilidade da matéria seca (CMMS), que foi maior nas dietas com inclusão de 2% de óleo de soja em relação à gordura de frango. A inclusão de 4% de lipídios na dieta, independente da fonte lipídica, melhorou o coeficiente de metabolizabilidade do extrato etéreo (CMEE) e aumentou o ganho de peso e o consumo de ração das aves. Ainda, na fase inicial, a adição de 4% de lipídios na dieta aumentou a atividade de lipase em relação às dietas com inclusões de 2%, observando-se uma correlação positiva entre o CMEE e a atividade de lipase pancreática. Como conclusão, não há interação entre as fontes lipídicas e os níveis de inclusão estudados, com ressalva para o CMMS. As características de carcaça não são influenciadas por nenhum dos fatores estudados. A adição de 4% de lipídio aumenta a atividade de lipase pancreática e melhora o CMEE, refletindo em maior ganho de peso das aves, indiferentemente da fonte lipídica testada, o que torna a gordura de frango uma boa alternativa ao óleo de soja...
Subject(s)
Animals , Animal Feed , Fatty Acids/administration & dosage , Chickens/growth & development , Soybean Oil/administration & dosage , Dietary Fats/administration & dosageABSTRACT
Using a semiotic and hermeneutical approach, the present paper discusses various theoretical elements in the analysis of popular views of schistosomiasis. Based on field work conducted in the State of Bahia, the authors identify two main processes in the construction of meaning related to schistosomiasis and show that depending on the social context of experience the disease may have a primary or secondary meaning for the populations affected by it. The first level of meaning refers to bodily experiences and is shared intersubjectively, whereas the second type is built on the discourse of health professionals. In the latter case, the popular view of the illness is directly linked to discourses and actions previously established by health programs designed for the control of the disease.
Subject(s)
Community Participation , Schistosomiasis mansoni/prevention & control , Adolescent , Attitude to Health , Brazil , Child , Child, Preschool , Health Education , Humans , Interviews as Topic , Rural PopulationABSTRACT
The results of the black light test for aflatoxin-contaminated maize carried out in a large food factory in the State of São Paulo was evaluated against bi-directional thin layer chromatography (TLC) analysis for 286 samples of maize. All 286 samples were accepted by the black light test (< 7 fluorescent points), however, the results from TLC analysis showed that 96 samples were contaminated and 14 showed aflatoxin B1 contamination levels higher than 20 micrograms/kg. There were 14 false negative results and no false positives and out of the 14 samples, six did not show visible fluorescent points. If the rejection criterion of one or more fluorescent points were applied, the six samples would be accepted by the black light test. But, in this case, 95 samples would be rejected and 87 results would be false positives because they did not have contamination levels over 20 micrograms/kg which is the acceptance limit of the black light test. The results indicate that the black light test, as utilized by this factory, was not able to indicate lots with possible contamination and the black light test, as recommended in the literature, would produce a high number of false positives. It is necessary to make more studies on the use of black light as a screening test for possible aflatoxin B1-contaminated maize.
Subject(s)
Aflatoxins/analysis , Food Contamination , Zea mays/chemistry , Brazil , Fluorescence , Ultraviolet RaysABSTRACT
This is a study on popular ideas, beliefs and practices concerning the transmission and prevention of parasitic diseases, specifically schistosomiasis mansoni. The author explored the existence of a popular belief system within the health care system, combining elements from biomedicine and folk medicine for the understanding and management of the health/disease phenomenon. The study describes the differences in perception of morbidity and gender among the interviewees. Finally, the study presents popular discourse on parasitic diseases and discusses difficulties with control measures. It considers the contradiction between cultural values and habits and conventional control measures, critically assessing the lack of precision in health education measures under government prevention programs.
ABSTRACT
A pesquisa incidiu sobre 170 ovelhas adultas da raça Corriedale, criadas em regime extensivo, sob moderadas condiçöes de nutriçäo, com peso médio de 35-40 kg, divididas em cinco grupos, sendo dois com estro induzido hormonalmente. O trabalho foi efetuado durante as estaçöes reprodutivas de 1986 e 1988, e os animais foram inseminados com sêmen fresco e congelado, pelas vias cervical e intra-uterina, esta mediante laparoscopia. A mortalidade embrionária foi medida indiretamente, pela relaçäo entre o número de fetos e o número de corpos lúteos encontrados nas ovelhas abatidas e que näo retornaram ao estro no prazo de 20 dias. A incidência da mortalidade embrionária mostrou-se equivalente quando se empregou o sêmen congelado pelas vias cervical e intra-uterina. O mesmo ocorreu com o uso do sêmen fresco e congelado pela via cervical, quando se utilizou o estro espontâneo. A mortalidade embrionária foi significativamente maior quando se aplicou sêmen congelado, tanto pela via cervical como intra-uterina, no estro induzido. O tratamento hormonal apesar de lograr aumento da taxa ovulatória näo influiu favoravelmente na prolificidade das ovelhas tratadas näo sendo por isso indicado para rebanhos que näo estejam em condiçöes nutritivas favoráveis
Subject(s)
Animals , Female , Pregnancy , Embryonic Structures , Insemination, Artificial , Mortality , SheepABSTRACT
Foram observados, na regiao de Ribeirao Preto, os valores sericos de zinco, cobre e ferro em 24 criancas com hemoglobinopatia (nove com anemia falciforme, 10 com beta-talassemia e cinco com S-talassemia) com idade compreendida entre seis e 144 meses. Os resultados mostraram que existe diminuicao da concentracao do zinco nestes pacientes, sendo mais evidente nas criancas com beta-talassemia. Ocorre aumento da concentracao do cobre serico.Ha aumento da concentracao do ferro serico apenas nas criancas com beta-talassemia. Nao foi encontrada correlacao entre os niveis de zinco e os de cobre e ferro, bem como entre os niveis de zinco e o deficit pondero-estatural
Subject(s)
Child, Preschool , Child , Humans , Male , Female , Anemia, Sickle Cell , Hemoglobinopathies , Thalassemia , ZincABSTRACT
Utilizando a tecnica de balanco metabolico estudou-se o comportamento do magnesio na fluidoterapia de criancas com desnutricao proteico-calorica de graus II e III (Marasmo). O balanco de magnesio foi negativo nas criancas que nao receberam este cation por via endovenosa e positivo nas que o receberam, sugerindo a necessidade de sua utilizacao em criancas desidratadas com este desvio nutricional
