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Pol J Vet Sci ; 15(2): 329-35, 2012.
Article in English | MEDLINE | ID: mdl-22844712

ABSTRACT

The Aerococcus viridans isolates from bovine mastitis in Slovakia were isolated and characterized by classical microbiological and biochemical, and molecular techniques including IGS-PCR and rep-PCR, ARDRA and 16S rDNA gene sequencing. The substantial variability of antibiotic resistance patterns was observed. The majority of strains were resistant to beta-lactam antibiotics, the resistance to tetracycline was observed in 3 tested strains, resistance to lincomycin was found in 4 strains and practically all tested strains were sensitive to neomycin and ciprofloxacin. While variable at a phenotypic level, no significant genetic variability among A. viridans isolates was detected by molecular DNA based methods. The data obtained suggest that a few A. viridans strains spread among cow's population in Slovak farms.


Subject(s)
Aerococcus/classification , Aerococcus/genetics , Genetic Variation , Gram-Positive Bacterial Infections/veterinary , Mastitis, Bovine/microbiology , Animals , Anti-Bacterial Agents/pharmacology , Cattle , DNA, Bacterial/genetics , Drug Resistance , Female , Gram-Positive Bacterial Infections/epidemiology , Gram-Positive Bacterial Infections/microbiology , Mastitis, Bovine/epidemiology , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Slovakia/epidemiology
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