ABSTRACT
According to the Segré-Silberberg effect, spherical particles migrate to a lateral equilibrium position in parabolic flow profiles. Here, for the first time, the corresponding equilibrium velocity is studied experimentally for micro particles in channels with rectangular cross section. Micro channels are fabricated in PMMA substrate based on a hot embossing process. To measure individual particle velocities at very high precision, the technique of spatially modulated emission is applied. It is found that the equilibrium velocity is size-dependent and the method offers a new way to measure particle size in microfluidic systems. The method is of particular interest for microfluidic flow cytometry as it delivers an alternative to the scatter signal for cell size determination.
ABSTRACT
In this paper, we describe the development of an automated sample preparation procedure for etiological agents of community-acquired lower respiratory tract infections (CA-LRTI). The consecutive assay steps, including sample re-suspension, pre-treatment, lysis, nucleic acid purification, and concentration, were integrated into a microfluidic lab-on-a-chip (LOC) cassette that is operated hands-free by a demonstrator setup, providing fluidic and valve actuation. The performance of the assay was evaluated on viral and Gram-positive and Gram-negative bacterial broth cultures previously sampled using a nasopharyngeal swab. Sample preparation on the microfluidic cassette resulted in higher or similar concentrations of pure bacterial DNA or viral RNA compared to manual benchtop experiments. The miniaturization and integration of the complete sample preparation procedure, to extract purified nucleic acids from real samples of CA-LRTI pathogens to, and above, lab quality and efficiency, represent important steps towards its application in a point-of-care test (POCT) for rapid diagnosis of CA-LRTI.
