ABSTRACT
In acute uremic rats (24 h after bilateral nephrectomy, serum urea 280-300 mg/dl) the interface tension of the serum is significantly reduced. Serum levels of triacylglycerol are significantly elevated in uremia, whereas cholesterol levels do not show a significant alteration. The in vitro serum binding reserve for both, triacylglycerol and cholesterol is considerably enhanced. These results let suppose the presence of tenside-like substances in uremic serum which may be involved in disturbed triacylglycerol transport from the serum to the tissues and in development of uremic hypertriglyceridemia.
Subject(s)
Cholesterol/blood , Triglycerides/blood , Uremia/blood , Acute Disease , Animals , Electrophoresis , Lipoproteins/blood , Lipoproteins/classification , Male , Nephrectomy , RatsABSTRACT
Intraperitoneal injection of oligomycin into the rat (0.5 mg per kg, corresponding to the LD33 dose) reduces the oxygen consumption by about 50%, whereas the arterial pO2 remains normal. The large extent of this decrease points to an involvement of liver and muscle tissue. Triiodothyronine pretreatment (3 doses of 0.075 mg/100 g body weight) is not able to prevent this effect. From the blood metabolites measured glucose, pyruvate and the parameters of lipid metabolism remain unchanged; only lactate is significantly increased, causing compensated metabolic acidosis. Heart rate, systolic blood pressure and electrocardiogram are essentially unchanged. Oliguria, reduced renal excretion of urea and increase of plasma urea also indicate a nephrotoxic action. The results are discussed in comparison with some effects of experimental uremia.
Subject(s)
Oligomycins/toxicity , Animals , Hemodynamics/drug effects , Kidney/drug effects , Mitochondria/drug effects , Oxygen Consumption/drug effects , Rats , Rats, Inbred StrainsABSTRACT
Adrenalin tablets (15 mg) or placebos were implanted into the neck of male Sprague-Dawley rats weighing between 150 to 250 g. 18 hours after implantation a significant lipolysis as well as enhanced concentrations of triacylglycerol in liver, kidney cortex and skeletal muscle can be observed in adrenalin treated rats. Measurements of fatty acid synthesis (3H method) in these tissues indicate that in kidney cortex and liver there is no contribution of local synthesis to elevated triacylglycerol content. In skeletal muscle local synthesis seems to be efficient to some degree. The main cause for lipid deposition in adrenalin supplied rats is, therefore, uptake of free fatty acids from the blood and following esterification with glycerol.