LAMS use in malignant stricture of duodenal apex with synchronous malignant biliary obstruction: a potentially effective and simpler endoscopic approach.

We report the first case of lumen apposing metal stent (LAMS) use in malignant stricture of the duodenal apex to treat gastric outlet obstruction (GOO) and to facilitate endoscopic retrograde management of synchronous malignant biliary obstruction due to pancreatic head adenocarcinoma. This technique may be an effective alternative and a simpler approach than current methodology to this endoscopically challenging condition.

Life cycle assessment of wheat production and wheat-based crop rotations.

In the northern Great Plains (NGP), wheat is the primary grain commodity. There is a need for the NGP to have a detailed analysis of environmental impacts for wheat-based agricultural production systems to better understand regional agroecosystems. This article provides a cradle-to-field gate life cycle assessment (LCA) for NGP dryland wheat (Triticum aestivum L.) production. The environmental impacts for winter wheat production using crop rotation and agricultural intensification are quantified. Fourteen no-till crop rotations ranging in duration from 2 to 6 yr were evaluated and compared using data from a historical 13-yr replicated rotation study (>300 observations). Midpoint LCA categories chosen for this comparison are energy, agricultural land use, climate change potential, freshwater eutrophication, and freshwater ecotoxicity due to their direct links with agricultural management practices. The NGP farmers commonly use a fallow period every other year due to moisture limitations. This specific agricultural practice and allocations within rotations are critical considerations within agricultural LCAs. Two aspects of fallow considerations and a sensitivity analysis were also performed. The allocated midpoint impacts between crops in rotational studies averaged 0.31, 0.79, 0.62, and 0.63 kg CO2 eq. per unit of winter wheat when energy, economic, mass, and cereal unit allocations were used, respectively. Economic analysis of the studied experimental crop was performed; results demonstrated that crop insurance policies improved diversification economics by 20%. Agricultural diversification benefits and burdens were better represented by endpoint damage assessments than by midpoint impact analysis.

Uncertainty and variability in laboratory derived sorption parameters of sediments from a uranium in situ recovery site.

This research assesses the ability of a GC SCM to simulate uranium transport under variable geochemical conditions typically encountered at uranium in-situ recovery (ISR) sites. Sediment was taken from a monitoring well at the SRH site at depths 192 and 193 m below ground and characterized by XRD, XRF, TOC, and BET. Duplicate column studies on the different sediment depths, were flushed with synthesized restoration waters at two different alkalinities (160 mg/l CaCO3 and 360 mg/l CaCO3) to study the effect of alkalinity on uranium mobility. Uranium breakthrough occurred 25% - 30% earlier in columns with 360 mg/l CaCO3 over columns fed with 160 mg/l CaCO3 influent water. A parameter estimation program (PEST) was coupled to PHREEQC to derive site densities from experimental data. Significant parameter fittings were produced for all models, demonstrating that the GC SCM approach can model the impact of carbonate on uranium in flow systems. Derived site densities for the two sediment depths were between 141 and 178 µmol-sites/kg-soil, demonstrating similar sorption capacities despite heterogeneity in sediment mineralogy. Model sensitivity to alkalinity and pH was shown to be moderate compared to fitted site densities, when calcite saturation was allowed to equilibrate. Calcite kinetics emerged as a potential source of error when fitting parameters in flow conditions. Fitted results were compared to data from previous batch and column studies completed on sediments from the Smith-Ranch Highland (SRH) site, to assess variability in derived parameters. Parameters from batch experiments were lower by a factor of 1.1 to 3.4 compared to column studies completed on the same sediments. The difference was attributed to errors in solid-solution ratios and the impact of calcite dissolution in batch experiments. Column studies conducted at two different laboratories showed almost an order of magnitude difference in fitted site densities suggesting that experimental methodology may play a bigger role in column sorption behavior than actual sediment heterogeneity. Our results demonstrate the necessity for ISR sites to remove residual pCO2 and equilibrate restoration water with background geochemistry to reduce uranium mobility. In addition, the observed variability between fitted parameters on the same sediments highlights the need to provide standardized guidelines and methodology for regulators and industry when the GC SCM approach is used for ISR risk assessments.

Potential water resource impacts of hydraulic fracturing from unconventional oil production in the Bakken shale.

Modern drilling techniques, notably horizontal drilling and hydraulic fracturing, have enabled unconventional oil production (UOP) from the previously inaccessible Bakken Shale Formation located throughout Montana, North Dakota (ND) and the Canadian province of Saskatchewan. The majority of UOP from the Bakken shale occurs in ND, strengthening its oil industry and businesses, job market, and its gross domestic product. However, similar to UOP from other low-permeability shales, UOP from the Bakken shale can result in environmental and human health effects. For example, UOP from the ND Bakken shale generates a voluminous amount of saline wastewater including produced and flowback water that are characterized by unusual levels of total dissolved solids (350 g/L) and elevated levels of toxic and radioactive substances. Currently, 95% of the saline wastewater is piped or trucked onsite prior to disposal into Class II injection wells. Oil and gas wastewater (OGW) spills that occur during transport to injection sites can potentially result in drinking water resource contamination. This study presents a critical review of potential water resource impacts due to deterministic (freshwater withdrawals and produced water management) and probabilistic events (spills due to leaking pipelines and truck accidents) related to UOP from the Bakken shale in ND.

Effect of structural carbohydrates and lignin content on the anaerobic digestion of paper and paper board materials by anaerobic granular sludge.

Anaerobic digestion (AD) of lignocellulosic materials is commonly limited by the hydrolysis step. Unlike unprocessed lignocellulosic materials, paper and paper board (PPB) are processed for their fabrication. Such modifications may affect their methane yields and methane production rates. Previous studies have investigated the correlation between lignin and biomethane yields of unprocessed lignocellulosic materials; nevertheless, there is limited knowledge regarding the relationship between the AD kinetic parameters and composition of PPB. This study evaluated correlations of methane yields and Monod and Gompertz kinetic parameters with structural carbohydrates, lignin, and ash concentration of five types of PPBs. All components were used as single and combined independent variables in linear regressions to predict methane yield, maximum specific methanogenic activity (SMAmax ), saturation constant (Ks ), and lag phase (λ). Additionally, microbial community profiles were obtained for each PPB assay. Results showed methane yields ranging from 69.2 ± 8.61 to 97.2 ± 2.29% of PPB substrates provided. The highest correlation coefficients were obtained for SMAmax as function of hemicellulose/(lignin + ash) (R2 = 0.86) and for λ as a function of lignin + cellulose (R2 = 0.85). All other parameters exhibited weaker correlations (R2 ≤ 0.77). Relative abundance analyses revealed no major changes in the community profile for each of the substrates evaluated. The overall findings of this study are: (i) combinations of structural carbohydrates, lignin, and ash used as ratios of degradable to either non-degradable or slowly degradable fractions predict AD kinetic parameters of PPB materials better than single independent variables; and (ii) other components added during their fabrication may also influence both methane yield and kinetic parameters. Biotechnol. Bioeng. 2017;114: 951-960. © 2016 Wiley Periodicals, Inc.

Eco-Efficiency Model for Evaluating Feedlot Rations in the Great Plains, United States.

Environmental impacts attributable to beef feedlot production provide an opportunity for economically linked efficiency optimization. Eco-efficiency models are used to optimize production and processes by connecting and quantifying environmental and economic impacts. An adaptable, objective eco-efficiency model was developed to assess the impacts of dietary rations on beef feedlot environmental and fiscal cost. The hybridized model used California Net Energy System modeling, life cycle assessment, principal component analyses (PCA), and economic analyses. The model approach was based on 38 potential feedlot rations and four transportation scenarios for the US Great Plains for each ration to determine the appropriate weight of each impact. All 152 scenarios were then assessed through a nested PCA to determine the relative contributing weight of each impact and environmental category to the overall system. The PCA output was evaluated using an eco-efficiency model. Results suggest that water, ecosystem, and human health emissions were the primary impact category drivers for feedlot eco-efficiency scoring. Enteric CH emissions were the greatest individual contributor to environmental performance (5.7% of the overall assessment), whereas terrestrial ecotoxicity had the lowest overall contribution (0.2% of the overall assessment). A well-balanced ration with mid-range dietary and processing energy requirements yielded the most eco- and environmentally efficient system. Using these results, it is possible to design a beef feed ration that is more economical and environmentally friendly. This methodology can be used to evaluate eco-efficiency and to reduce researcher bias of other complex systems.

Batch anaerobic digestion of synthetic military base food waste and cardboard mixtures.

Austere US military bases typically dispose of solid wastes, including large fractions of food waste (FW) and corrugated cardboard (CCB), by open dumping, landfilling, or burning. Anaerobic digestion (AD) offers an opportunity to reduce pollution and recover useful energy. This study aimed to evaluate the rates and yields of AD for FW-CCB mixtures. Batch AD was analyzed at substrate concentrations of 1-50g total chemical oxygen demand (COD)L(-1) using response surface methodology. At low concentrations, higher proportions of FW were correlated with faster specific methanogenic activities and greater final methane yields; however, concentrations of FW ⩾18.75gCODL(-1) caused inhibition. Digestion of mixtures with ⩾75% CCB occurred slowly but achieved methane yields >70%. Greater shifts in microbial communities were observed at higher substrate concentrations. Statistical models of methane yield and specific methanogenic activity indicated that FW and CCB exhibited no considerable interactions as substrates for AD.

Life-Cycle Assessment of Oilseeds for Biojet Production Using Localized Cold-Press Extraction.

As nonfood oilseed varieties are being rapidly developed, new varieties may affect agricultural production efficiency and life-cycle assessment results. Current, detailed feedstock production information is necessary to accurately assess impacts of the biofuel life-cycle. The life-cycle impacts of four nonfood oilseeds (carinata [ L. Braun], camelina [ L. Crantz], canola or rapeseed [ L.], and sunflower [ L.]) were modeled using Argonne National Laboratory's GREET model to compare feedstocks for renewable biojet production using cold-press oil extraction. Only feedstock-related inputs were varied, allowing isolation of feedstock influence. Carinata and camelina performed slightly better than other oilseed crops at most product stages and impact categories as a result of current, low-input agricultural information and new feedstock varieties. Between 40 to 50% of SO and NO emissions, ∼25% of greenhouse gas (GHG) emissions, and ∼40% of total energy consumption for the biojet production impact occurred during feedstock production. Within the first standard deviation, total well-to-tank emissions varied between ∼13% (GHG) and ∼35% (SO) for all feedstocks emphasizing the importance of accurate agricultural production information. Nonfood oilseed feedstock properties (e.g., oil content, density) and agricultural management (e.g., fertilization, yield) affect life-cycle assessment results. Using biofuels in feedstock production and focusing on low-impact management would assist producers in improving overall product sustainability.

Meta-Analysis of Soybean-based Biodiesel.

Biofuel policy changes in the United States have renewed interest in soybean [ (L.) Merr.] biodiesel. Past studies with varying methodologies and functional units can provide valuable information for future work. A meta-analysis of nine peer-reviewed soybean life cycle analysis (LCA) biodiesel studies was conducted on the northern Great Plains in the United States. Results of LCA studies were assimilated into a standardized system boundary and functional units for global warming (GWP), eutrophication (EP), and acidification (AP) potentials using biodiesel conversions from peer-reviewed and government documents. Factors not fully standardized included variations in NO accounting, mid- or end-point impacts, land use change, allocation, and statistical sampling pools. A state-by-state comparison of GWP lower and higher heating values (LHV, HHV) showed differences attributable to variations in spatial sampling and agricultural practices (e.g., tillage, irrigation). The mean GWP of LHV was 21.1 g·CO-eq MJ including outliers, and median EP LHV and AP LHV was 0.019 g·PO-eq MJ and 0.17 g·SO-eq MJ, respectively, using the limited data available. An LCA case study of South Dakota soybean-based biodiesel production resulted in GWP estimates (29 or 31 g·CO-eq MJ; 100% mono alkyl esters [first generation] biodiesel or 100% fatty acid methyl ester [second generation] biodiesel) similar to meta-analysis results (30.1 g·CO-eq MJ). Meta-analysis mean results, including outliers, resemble the California Low Carbon Fuel Standard for soybean biodiesel default value without land use change of 21.25 g·CO-eq MJ. Results were influenced by resource investment differences in water, fertilizer (e.g., type, application), and tillage. Future biofuel LCA studies should include these important factors to better define reasonable energy variations in regional agricultural management practices.

Tillage and corn residue harvesting impact surface and subsurface carbon sequestration.

Corn stover harvesting is a common practice in the western U.S. Corn Belt. This 5-yr study used isotopic source tracking to quantify the influence of two tillage systems, two corn ( L.) surface residue removal rates, and two yield zones on soil organic C (SOC) gains and losses at three soil depths. Soil samples collected in 2008 and 2012 were used to determine C enrichment during SOC mineralization, the amount of initial SOC mineralized (SOC), and plant C retained in the soil (PCR) and sequestered C (PCR - SOC). The 30% residue soil cover after planting was achieved by the no-till and residue returned treatments and was not achieved by the chisel plow, residue removed treatment. In the 0- to 15-cm soil depth, the high yield zone had lower SOC (1.49 Mg ha) than the moderate yield zone (2.18 Mg ha), whereas in the 15- to 30-cm soil depth, SOC was higher in the 60% (1.38 Mg ha) than the 0% (0.82 Mg ha) residue removal treatment. When the 0- to 15- and 15- to 30-cm soil depths were combined, (i) 0.91 and 3.62 Mg SOC ha were sequestered in the 60 and 0% residue removal treatments; (ii) 2.51 and 0.36 Mg SOC ha were sequestered in the no-till and chisel plow treatments, and (iii) 1.16 and 1.65 Mg SOC ha were sequestered in the moderate and high yield zone treatments, respectively. The surface treatments influenced C cycling in the 0- to 15- and 15- to 30-cm depths but did not influence SOC turnover in the 30- to 60-cm depth.

Life Cycle Assessment modelling of stormwater treatment systems.

Stormwater treatment technologies to manage runoff during rain events are primarily designed to reduce flood risks, settle suspended solids and concurrently immobilise metals and nutrients. Life Cycle Assessment (LCA) is scarcely documented for stormwater systems despite their ubiquitous implementation. LCA modelling quantified the environmental impacts associated with the materials, construction, transport, operation and maintenance of different stormwater treatment systems. A pre-fabricated concrete vortex unit, a sub-surface sandfilter and a raingarden, all sized to treat a functional unit of 35 m(3) of stormwater runoff per event, were evaluated. Eighteen environmental mid-point metrics and three end-point 'damage assessment' metrics were quantified for each system's lifecycle. Climate change (kg CO2 eq.) dominated net environmental impacts, with smaller contributions from human toxicity (kg 1,4-DB eq.), particulate matter formation (kg PM10 eq.) and fossil depletion (kg oil eq.). The concrete unit had the highest environmental impact of which 45% was attributed to its maintenance while impacts from the sandfilters and raingardens were dominated by their bulky materials (57%) and transport (57%), respectively. On-site infiltrative raingardens, a component of green infrastructure (GI), had the lowest environmental impacts because they incurred lower maintenance and did not have any concrete which is high in embodied CO2. Smaller sized raingardens affording the same level of stormwater treatment had the lowest overall impacts reinforcing the principle that using fewer resources reduces environmental impacts. LCA modelling can serve as a guiding tool for practitioners making environmentally sustainable solutions for stormwater treatment.

Life-cycle assessment of the beef cattle production system for the northern great plains, USA.

A life-cycle assessment (LCA) model was developed to estimate the environmental impacts associated with four different U.S. Northern Great Plains (NPG) beef production systems. The LCA model followed a "cradle-to-gate" approach and incorporated all major unit processes, including mineral supplement production. Four distinct operation scenarios were modeled based on production strategies common to the NGP, and a variety of impacts were determined. The scenarios include a normal operation, early weaning of the calf, fast-tack backgrounding, and grassfed. Enteric emissions and manure emissions and handling were consistently the largest contributors to the LCA impacts. There was little variability between production scenarios except for the grassfed, where the greenhouse gas (GHG) emissions were 37% higher due to a longer finishing time and lower finishing weight. However, reductions to GHG emissions (15-24%) were realized when soil organic carbon accrual was considered and may be a more realistic estimate for the NGP. Manure emissions and handing were primary contributors to potential eutrophication and acidification impacts. Mitigation strategies to reduce LCA impacts, including diet manipulation and management strategies (i.e., treatment of manure), were considered from a whole-systems perspective. Model results can be used for guidance by NGP producers, environmental practitioners, and policymakers.

Effects of chlortetracycline amended feed on anaerobic sequencing batch reactor performance of swine manure digestion.

The effects of antimicrobial chlortetracycline (CTC) on the anaerobic digestion (AD) of swine manure slurry using anaerobic sequencing batch reactors (ASBRs) was investigated. Reactors were loaded with manure collected from pigs receiving CTC and no-antimicrobial amended diets at 2.5 g/L/d. The slurry was intermittently fed to four 9.5L lab-scale anaerobic sequencing batch reactors, two with no-antimicrobial manure, and two with CTC-amended manure, and four 28 day ASBR cycles were completed. The CTC concentration within the manure was 2 8 mg/L immediately after collection and 1.02 mg/L after dilution and 250 days of storage. CTC did not inhibit ASBR biogas production extent, however the volumetric composition of methane was significantly less (approximately 13% and 15% for cycles 1 and 2, respectively) than the no-antimicrobial through 56 d. CTC decreased soluble chemical oxygen demand and acetic acid utilization through 56 d, after which acclimation to CTC was apparent for the duration of the experiment.

Land application of tylosin and chlortetracycline swine manure: Impacts to soil nutrients and soil microbial community structure.

The land application of aged chortetracycle (CTC) and tylosin-containing swine manure was investigated to determine associated impacts to soil microbial respiration, nutrient (phosphorus, ammonium, nitrate) cycling, and soil microbial community structure under laboratory conditions. Two silty clay loam soils common to southeastern South Dakota were used. Aerobic soil respiration results using batch reactors containing a soil-manure mixture showed that interactions between soil, native soil microbial populations, and antimicrobials influenced CO(2) generation. The aged tylosin treatment resulted in the greatest degree of CO(2) inhibition, while the aged CTC treatment was similar to the no-antimicrobial treatment. For soil columns in which manure was applied at a one-time agronomic loading rate, there was no significant difference in soil-P behavior between either aged CTC or tylosin and the no-antimicrobial treatment. For soil-nitrogen (ammonium and nitrate), the aged CTC treatment resulted in rapid ammonium accumulation at the deeper 40cm soil column depth, while nitrate production was minimal. The aged CTC treatment microbial community structure was different than the no-antimicrobial treatment, where amines/amide and carbohydrate chemical guilds utilization profile were low. The aged tylosin treatment also resulted in ammonium accumulation at 40 cm column depth, however nitrate accumulation also occurred concurrently at 10 cm. The microbial community structure for the aged tylosin was also significantly different than the no-antimicrobial treatment, with a higher degree of amines/amides and carbohydrate chemical guild utilization compared to the no-antimicrobial treatment. Study results suggest that land application of CTC and tylosin-containing manure appears to fundamentally change microbial-mediated nitrogen behavior within soil A horizons.

Impact of chlortetracycline on sequencing batch reactor performance for swine manure treatment.

Treatment of aged (500 day, 4°C stored) chlortetracycline (CTC; 0, 20, 40, 80 mg/L CTC)-amended swine manure using two cycle, 22 day stage anaerobic sequencing batch reactors (SBR) was assessed. Eighty milligrams per liter CTC treatment inhibited SBR treatment efficiencies, although total gas production was enhanced compared to the no-CTC treatment. The 20 and 40 mg/L CTC treatments resulted in either slight or no differences to SBR treatment efficiencies and microbial diversities compared to the no-CTC treatment, and were generally similar to no-CTC treatments upon completion of the first 22 day SBR cycle. All CTC treatments enhanced SBR gas generation, however CH(4) yields were lowest for the 80 mg/L CTC treatment (0.111L CH(4)/g tCOD) upon completion of the second SBR react cycle. After a 22 day acclimation period, the 80 mg/L CTC treatment inhibited methanogenesis due to acetate accumulation, and decreased microbial diversity and CH(4) yield compared to the no-CTC treatment.

Influence of physiochemical and watershed characteristics on mercury concentration in Walleye, Sander vitreus, M.

Elevated mercury concentration has been documented in a variety of fish and is a growing concern for human consumption. Here, we explore the influence of physiochemical and watershed attributes on mercury concentration in walleye (Sander vitreus, M.) from natural, glacial lakes in South Dakota. Regression analysis showed that water quality attributes were poor predictors of walleye mercury concentration (R² = 0.57, p = 0.13). In contrast, models based on watershed features (e.g., lake level changes, watershed slope, agricultural land, wetlands) and local habitat features (i.e., substrate composition, maximum lake depth) explained 81% (p = 0.001) and 80% (p = 0.002) of the variation in walleye mercury concentration. Using an information theoretic approach we evaluated hypotheses related to water quality, physical habitat and watershed features. The best model explaining variation in walleye mercury concentration included local habitat features (W(i) = 0.991). These results show that physical habitat and watershed features were better predictors of walleye mercury concentration than water chemistry in glacial lakes of the Northern Great Plains.

Tylosin and chlortetracycline effects during swine manure digestion: influence of sodium azide.

The antibiotics tylosin and chlortetracycline (CTC), which are commonly used in pig production, were studied to determine their effects on swine manure digestion in the presence and absence of biocide sodium azide. CTC enhanced initial hydrolysis reactions through volatile suspended solids production, while inhibiting methane and carbon dioxide production. Tylosin did not affect methane and carbon dioxide production; however, the relative abundance of both hydrogen utilizing and acetate-only utilizing microbial populations was significantly compromised. Sodium azide in the absence of antibiotics enhanced metabolic output and initial biomass production, and this observation suggests that populations of Methanobacteriales and Methanosaetaceae spp. appeared to contain sufficient periplasmic bound reductase to effectively utilize acetate and hydrogen in the presence of sodium azide. However, the combination of sodium azide and either CTC or tylosin was a very effective metabolic inhibitor, inhibiting methane and carbon dioxide production and VSS consumption compared to their no-azide counterpart.

Directional fluid flow enhances in vitro periosteal tissue growth and chondrogenesis on poly-epsilon-caprolactone scaffolds.

The purpose of this study was to investigate the effect of directional fluid flow on periosteal chondrogenesis. Periosteal explants were harvested from 2-month-old rabbits and sutured onto poly-epsilon-caprolactone (PCL) scaffolds with the cambium layer facing away from the scaffolds. The periosteum/PCL composites were cultured in suspension in spinner flask bioreactors and exposed to various fluid flow velocities: 0, 20, 60, and 150 rpm for 4 h each day for 6 weeks. The application of fluid flow significantly increased percent cartilage yield in periosteal explants from 17% in the static controls to 65-75% under fluid flow (there was no significant difference between 20, 60, or 150 rpm). The size of the neocartilage was also significantly greater in explants exposed to fluid flow compared with static culture. The development of zonal organization within the engineered cartilage was observed predominantly in the tissue exposed to flow conditions. The Young's modulus of the engineered cartilage exposed to 60 rpm was significantly greater than the samples exposed to 150 and 20 rpm. These results demonstrate that application of directional fluid flow to periosteal explants secured onto PCL scaffolds enhances cell proliferation, chondrogenic differentiation, and cell organization and alters the biomechanical properties of the engineered cartilage.

Effect of antimicrobial compounds tylosin and chlortetracycline during batch anaerobic swine manure digestion.

Tylosin and chlortetracycline (CTC) are antimicrobial chemicals that are fed to >45% of the US swine herds at therapeutic and sub-therapeutic dosages to enhance growth rates and treat swine health problems. These compounds are poorly absorbed during digestion so that the bioactive compound or metabolites are excreted. This study investigated the degradation and stabilization of swine manure that contained no additives and compared the observed processes with those of manure containing either tylosin or CTC. The batch anaerobic incubation lasted 216 days. The breakdown of insoluble organic matter through anaerobic hydrolysis reactions was faster for manure containing CTC compared with tylosin or no-antimicrobial treatments. Volatile fatty acid (VFA) accumulation, including acetate, butyrate, and propionate, was greater for CTC-containing manure compared to tylosin and no-antimicrobial treatments. The relative abundance of two aceticlastic methanogens, Methanosaetaceae and Methanosarcinaceae spp., were less for CTC manure than manure with no-antimicrobial treatment. In addition, generation of methane and carbon dioxide was inhibited by 27.8% and 28.4%, respectively, due to the presence of CTC. Tylosin effects on manure degradation were limited, however the relative abundance of Methanosarcinaceae spp. was greater than found in the CTC or no-antimicrobial manures. These data suggest that acetate and other C-1 VFA compounds would be effectively utilized during methanogenesis in the presence of tylosin.

Effect of natural organic matter on zinc inhibition of hematite bioreduction by Shewanella putrefaciens CN32.

The effect of zinc on the biological reduction of hematite (alpha-Fe2O3) by the dissimilatory metal-reducing bacterium (DMRB) Shewanella putrefaciens CN32 was studied in the presence of four natural organic materials (NOMs). Experiments were performed under non-growth conditions with H2 as the electron donor and zinc inhibition was quantified as the decrease in the 5 d extent of hematite bioreduction as compared to no-zinc controls. Every NOM was shown to significantly increase zinc inhibition during hematite bioreduction. NOMs were shown to alter the distribution of both biogenic Fe(II) and Zn(II) between partitioned (hematite and cell surfaces) and solution phases. To further evaluate the mechanism(s) of NOM-promoted zinc inhibition, similar bioreduction experiments were conducted with nitrate as a soluble electron acceptor, and hematite bioreduction experiments were conducted with manganese which was essentially non-inhibitory in the absence of NOM. The results suggest that Me(II)-NOM complexes may be specifically inhibitory during solid-phase bioreduction via interference of DMRB attachment to hematite through the formation of ternary Me(II)-NOM-hematite complexes.