ABSTRACT
BACKGROUND: Over the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto-antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA-antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation. METHODS: Blood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti-dsDNA, anti-ssDNA, anti-nucleosome, anti-histone antibodies as well as for cell-free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated. RESULTS: As demonstrated herein, compared to healthy subjects, cell-free DNA plasma levels in patients with SLE were significantly increased and so were anti-dsDNA, anti-ssDNA, anti-histone and anti-nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell-free DNA and anti-histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti-dsDNA, anti-ssDNA and anti-nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell-free DNA levels and SLEDAI scores. CONCLUSIONS: The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results.
Subject(s)
Antibodies, Antinuclear/analysis , DNA/blood , Lupus Erythematosus, Systemic/immunology , Antibodies, Antinuclear/immunology , Case-Control Studies , DNA/analysis , DNA/immunology , Enzyme-Linked Immunosorbent Assay , Histones/immunology , Humans , Lupus Erythematosus, Systemic/blood , Nucleosomes/immunology , Plasma/immunology , Severity of Illness Index , Statistics as TopicABSTRACT
A stress response has the potential to induce greater resistance to subsequent stress damage. We tested whether hydrogen sulfide (H(2)S), an important signaling molecule, also used therapeutically, and known for detrimental effects, might induce a protective stress response. Therefore, the response of fibroblast-like synoviocytes (FLS) treated with sodium hydrosulfide and mice exposed to H(2)S were examined. In both cases a profound and long lasting induction of the stress-response could be detected. However, despite the sustained presence of large levels of HO-1 and HSP-70, proinflammatory effects of exposure to IL-1beta or H(2)S itself were not ameliorated. On the contrary, at H(2)S concentrations significantly lower than 10 ppm-the current maximal allowable concentration of H(2)S in many countries-COX-2, IL-8, IL-1alpha, IL-1beta and TNFalpha were dose dependently elevated. Importantly, in FLS, short-term exposure to H(2)S resulted in the activation of all three MAPK. In addition, mitochondrial activity was also significantly impaired at relatively low H(2)S concentrations. The transcription factor NF-kappaB is essential for the activation of most proinflammatory genes. However, the data presented imply that H(2)S activates proinflammatory genes in FLS through non-NF-kappaB-dependent pathways. Stress proteins reportedly act by blocking NF-kappaB activation, a mechanism that would explain the inability of stress proteins to prevent H(2)S mediated inflammatory processes. The presented data, showing MAPK activation, NF-kappaB-independent activation of a number of proinflammatory genes and mitochondrial damage, help to provide a better understanding of the biological and pathophysiological effects of exposure to H(2)S.
Subject(s)
Hydrogen Sulfide/pharmacology , Inflammation Mediators/metabolism , Inflammation/genetics , Transcriptional Activation/drug effects , Animals , Enzyme Activation/drug effects , Fibroblasts/drug effects , Fibroblasts/enzymology , Fibroblasts/pathology , Humans , Interleukin-1beta/metabolism , Mice , Mitochondria/drug effects , Mitochondria/metabolism , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , Phosphorylation/drug effects , Protein Transport/drug effects , RNA, Messenger/genetics , RNA, Messenger/metabolism , Signal Transduction/drug effects , Stress, Physiological/drug effects , Stress, Physiological/genetics , Synovial Fluid/cytology , Synovial Fluid/drug effects , Synovial Fluid/enzymology , Time FactorsABSTRACT
Liver X receptors (LXRs) are nuclear receptors regulating lipid and cholesterol metabolism. Recent data indicate an additional role of LXR in immunity by controlling dendritic cell and T-cell function and in breast and prostate cancer cells. Here, we show that LXR activation interferes with IL-2 and IL-7-induced proliferation and cell cycle progression of human T-cell blasts mainly through inhibited phosphorylation of the retinoblastoma protein and decreased expression of the cell cycle protein cyclin B. Comparable results were obtained with IL-2-dependent chronic lymphoblastic leukemia (CLL) T cells. Furthermore, we show for B-CLL cells that LXR are functionally active and inhibit expression of survival genes bcl-2 and MMP-9, and significantly reduce cell viability, suggesting an interference of LXR with cytokine-dependent CLL cell survival. In conclusion, our data reveal LXR as a potent modulator of cytokine-dependent proliferation and survival of normal and malignant T and B lymphocytes. This novel LXR action could find clinical application in immunosuppressive and antileukemic therapies.
Subject(s)
Cytokines/pharmacology , Leukemia, Lymphocytic, Chronic, B-Cell/immunology , Leukemia/pathology , Lymphocytes/immunology , Lymphocytes/pathology , Orphan Nuclear Receptors/physiology , Antigens, CD/immunology , Cell Division/drug effects , Cell Survival/drug effects , Cholesterol/metabolism , Humans , Leukemia/immunology , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Leukemia, Prolymphocytic, T-Cell/immunology , Leukemia, Prolymphocytic, T-Cell/pathology , Lipids/physiology , Liver X Receptors , Lymphocytes/cytology , Lymphocytes/drug effects , Methylphenazonium Methosulfate/pharmacology , Orphan Nuclear Receptors/immunology , T-Lymphocytes/immunologyABSTRACT
We demonstrated earlier that hyperthermia (HT), a form of balneotherapy, suppresses transcription and translation of a number of pro-inflammatory genes. Here we show that short term HT not only acts by preventing the activation of NF-kappaB, but also by blocking the activation of the MAPK p38. Data are presented that show that the effects of HT on p38 are clearly independent of HT effects on NF-kappaB. This is demonstrated by the ability of short term HT to prevent IL-1beta induced activation of MAPK p38 dependent, but NF-kappaB independent genes. In general it is assumed that the protective effects of HT are mediated by de novo synthesis of a number of heat shock proteins. However, data presented herein imply the need to distinguish early and late acting mechanisms of short term HT. Neither a recovery period nor de novo protein synthesis is essential for the early protective effects of HT. The effectiveness of short term HT in preventing the activation of two important signaling pathways and the relative ease by which the temperature in joints of arthritis patients might be modulated, seemingly offer an appealing opportunity to prevent or diminish inflammation by balneological means.
Subject(s)
Arthritis/therapy , Inflammation Mediators/metabolism , NF-kappa B/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism , Arthritis/genetics , Blotting, Western , Cell Line , Gene Expression Regulation , Humans , Hyperthermia, Induced/methods , NF-kappa B/genetics , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction/drug effects , p38 Mitogen-Activated Protein Kinases/geneticsABSTRACT
BACKGROUND: Aim of this study was to establish the method yielding the highest sensitivity routinely used to determine fetal RhD type and gender from maternal cell-free plasma DNA in different periods of gestation. METHODS: Plasma DNA concentrations were measured from 46 pregnant women in different gestational periods and tested for RhD using three different PCR methods on exon 7: Thermal Cycler, Taqman method on LightCycler, and melting curve analysis on LightCycler. In addition, fetal gender was determined by PCR. Cell-free plasma DNA was measured in 100 healthy volunteers as a reference group. RESULTS: The mean value of cell-free plasma DNA in the reference group was 10.9 pg/microL mean, (standard deviation (SD): 3.66) in 50 healthy women and 12.7 pg/microL (SD: 8.2) in 50 healthy men. In the first trimester of pregnancy cell-free plasma DNA was 14.9 pg/microL mean, (SD: 4.2), in the second trimester 15.4 pg/microL mean, (SD: 4.96), and the maximum was achieved in the third trimester of pregnancy 15.6 pg/microl mean, (SD: 6.49). TaqMan probes had the same accuracy, when compared with Thermal Cycler technology (46 samples, 6 failures). Using real-time PCR with melting curve analysis 12 of 17 samples were correctly tested. Gender determination was correctly in 41 of 46 samples. CONCLUSION: RhD determinations with TaqMan and Thermal Cycler technology are useful methods for fetal RhD prediction. To increase the accuracy of RhD determination it is necessary to test on other exons in addition.
Subject(s)
DNA/blood , Polymerase Chain Reaction/methods , Prenatal Diagnosis/methods , Rh-Hr Blood-Group System/blood , Adult , Diagnostic Errors , Female , Humans , Male , Middle Aged , Predictive Value of Tests , Pregnancy , Rh Isoimmunization/genetics , Rh Isoimmunization/prevention & control , Rh-Hr Blood-Group System/genetics , Sensitivity and Specificity , Sex Determination Analysis/methods , Statistics, NonparametricABSTRACT
The innate inflammatory immune response must be tightly controlled to avoid damage to the host. Here, we showed that the tuberous sclerosis complex-mammalian target of rapamycin (TSC-mTOR) pathway regulated inflammatory responses after bacterial stimulation in monocytes, macrophages, and primary dendritic cells. Inhibition of mTOR by rapamycin promoted production of proinflammatory cytokines via the transcription factor NF-kappaB but blocked the release of interleukin-10 via the transcription factor STAT3. Conversely, deletion of TSC2, the key negative regulator of mTOR, diminished NF-kappaB but enhanced STAT3 activity and reversed this proinflammatory cytokine shift. Rapamycin-hyperactivated monocytes displayed a strong T helper 1 (Th1) cell- and Th17 cell-polarizing potency. Inhibition of mTOR in vivo regulated the inflammatory response and protected genetically susceptible mice against lethal Listeria monocytogenes infection. These data identify the TSC2-mTOR pathway as a key regulator of innate immune homeostasis with broad clinical implications for infectious and autoimmune diseases, vaccination, cancer, and transplantation.
Subject(s)
Cytokines/immunology , Immunity, Innate , Listeriosis/immunology , Monocytes/immunology , Protein Kinases/metabolism , Tumor Suppressor Proteins/metabolism , Animals , Anti-Bacterial Agents/pharmacology , Cytokines/biosynthesis , Female , Humans , Inflammation/immunology , Inflammation/metabolism , Lipopolysaccharides/immunology , Listeria monocytogenes/immunology , Listeriosis/microbiology , Listeriosis/prevention & control , Mice , Mice, Inbred BALB C , Mice, Knockout , Monocytes/drug effects , Monocytes/metabolism , NF-kappa B/metabolism , Protein Kinases/immunology , STAT3 Transcription Factor/metabolism , Signal Transduction , Sirolimus/pharmacology , TOR Serine-Threonine Kinases , Th1 Cells/immunology , Th1 Cells/metabolism , Tuberous Sclerosis , Tuberous Sclerosis Complex 2 ProteinABSTRACT
We investigated the effects of an ultra-marathon on cell-free plasma DNA as well as on mRNA expression of pro-apoptotic (Bax, Bad), anti-apoptotic (Bcl-2) and cell-protective (Hsp70, Hsp27 and Hsp32) genes in mononuclear blood cells (MNCs). Blood samples were drawn from 14 athletes before and immediately after 6-h run. In addition, blood samples were also collected and analyzed 2 and 24 h after the end of the run. Levels of plasma DNA were significantly increased immediately after the marathon (P < 0.001) and were still higher 2 h later (P < 0.005), but significantly lower than those immediately after the race (P < 0.05). Cell-free plasma DNA returned to pre-race levels 24 h after the run. mRNA expressions of Hsp70, Hsp32 and Bax significantly increased in MNCs after the race, whereas Hsp27 and Bad mRNA expression levels showed no significant changes. Bcl-2 expressions decreased immediately after the race (P < 0.001), but increased in the 24 h later (P < 0.05). We conclude that apoptotic ladders of cell-free DNA following exhaustive exercise originate from apoptotic cells and that not only skeletal muscle cells but also leukocytes contribute to this phenomenon.
Subject(s)
Apoptosis/genetics , DNA/blood , Leukocytes, Mononuclear/metabolism , RNA, Messenger/metabolism , Running/physiology , Adult , Female , HSP27 Heat-Shock Proteins/genetics , HSP27 Heat-Shock Proteins/metabolism , HSP70 Heat-Shock Proteins/genetics , HSP70 Heat-Shock Proteins/metabolism , Heme Oxygenase-1/genetics , Heme Oxygenase-1/metabolism , Humans , Leukocytes, Mononuclear/cytology , Male , Middle Aged , Muscle, Skeletal/cytology , Muscle, Skeletal/metabolism , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Time Factors , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/metabolism , bcl-Associated Death Protein/genetics , bcl-Associated Death Protein/metabolismABSTRACT
One of the hallmarks of arthritis is swollen joints containing unusually high quantities of hyaluronan. Intact hyaluronan molecules facilitate cell migration by acting as ligands for CD44. Hyaluronan degradation products, readily formed at sites of inflammation, also fuel inflammatory processes. Irrespective of whether viruses could be a cause of rheumatoid arthritis, there is clear evidence that links viral infections to this debilitating disease. For this study, live Epstein-Barr virus and a number of double- and single-stranded synthetic viral analogs were tested for their effectiveness as activators of hyaluronan (HA) synthesis. As shown herein, Epstein-Barr virus-treated fibroblast-like synoviocytes significantly increase HA production and release. Real time reverse transcription-PCR data show that HAS1 mRNA levels are significantly elevated in virus-treated cells, whereas mRNA levels for the genes HAS2 and HAS3 remain unchanged. As to the mechanism of virus-induced HAS1 transcription, data are presented that imply that among the double- and single-stranded polynucleotides tested, homopolymeric polycytidylic structures are the most potent inducers of HAS1 transcription and HA release, whereas homopolymeric polyinosinic acid is without effect. Analyses of virus-induced signal cascades, utilizing chemical inhibitors of MAPK and overexpressing mutated IKK and IkappaB, revealed that the MAPK p38 as well as the transcription factor NF-kappaB are essential for virus-induced activation of HAS1. The presented data implicate HAS1 as the culprit in unfettered HA release and point out targets in virus-induced signaling pathways that might allow for specific interventions in cases of unwanted and uncontrolled HA synthesis.
Subject(s)
Gene Expression Regulation , Glucuronosyltransferase/metabolism , Herpesvirus 4, Human/metabolism , Hyaluronic Acid/metabolism , RNA, Double-Stranded/chemistry , RNA, Viral/chemistry , Synovial Membrane/cytology , Humans , Hyaluronan Synthases , Hyaluronic Acid/chemistry , MAP Kinase Signaling System , Models, Biological , NF-kappa B/metabolism , Oligonucleotides/chemistry , Polynucleotides/chemistry , Thy-1 Antigens/biosynthesis , Virus Diseases/metabolismABSTRACT
Many alternative treatment approaches, originating from Asia, are becoming increasingly popular in the Western hemisphere. Recently, an article published in a renowned journal reported that venom of apis mellifera (bee venom (BV)) and melittin mediate immune-modulating effects by blocking the activation of the transcription factor NF-kappaB. Such a modus operandi would corroborate the many claims of beneficial effects of BV treatment and give immediate credit to this form of therapy. Fibroblast-like synoviocytes from rheumatoid arthritis patients and dermal fibroblast cells and white blood cells from healthy volunteers were used to study the effects of BV and melittin on the activation of NF-kappaB and a series of genes that are markers of inflammation. EMSAs demonstrate that neither BV nor melittin blocked IL-1beta-induced NF-kappaB activation; neither did they affect phosphorylation or degradation of IkappaB. Contrary to published data, even high concentrations of BV and melittin were without any effect on NF-kappaB-p50-DNA interactions. More importantly, in fibroblast-like synoviocytes, but also in dermal fibroblasts as well as in mononuclear cells exposed to BV or melittin, mRNA levels of several proinflammatory genes are significantly increased, and Western blot data show elevated cyclooxygenase-2 protein levels. Furthermore, exposure to BV higher than 10 mug/ml resulted in disintegration of all cell types tested. In addition, large quantities of oxygen radicals are produced in a dose-dependent manner in leukocytes exposed to BV. Taken together, data presented in this work do not corroborate an earlier report regarding the effectiveness of BV as an inhibitor of the transcription factor NF-kappaB.
Subject(s)
Bee Venoms/pharmacology , DNA/metabolism , Gene Expression Regulation/immunology , Melitten/physiology , NF-kappa B p50 Subunit/metabolism , Reactive Oxygen Species/metabolism , Transcriptional Activation/immunology , Apoptosis/genetics , Apoptosis/immunology , Cell Membrane/immunology , Cell Membrane/metabolism , Cell Membrane/pathology , Cells, Cultured , Cyclooxygenase 2/biosynthesis , Cyclooxygenase 2/genetics , DNA/antagonists & inhibitors , DNA/physiology , Enzyme Activation/immunology , Fibroblasts/immunology , Fibroblasts/metabolism , Fibroblasts/pathology , Humans , I-kappa B Proteins/antagonists & inhibitors , I-kappa B Proteins/metabolism , Interleukin-1beta/antagonists & inhibitors , Interleukin-1beta/physiology , Mitogen-Activated Protein Kinases/metabolism , NF-KappaB Inhibitor alpha , NF-kappa B p50 Subunit/antagonists & inhibitors , NF-kappa B p50 Subunit/physiology , Necrosis , Phosphorylation , Protein Binding/immunology , Synovial Membrane/immunology , Synovial Membrane/metabolism , Synovial Membrane/pathologyABSTRACT
We demonstrated earlier that the gene HAS1 is inactive in resting type-B-synoviocytes but can be readily activated by a series of proinflammatory cytokines including IL-1beta. Here we show that in type-B-synoviocytes mRNA levels for the gene COX-2 increase more than 200-fold in response to IL-1beta treatment, whereas COX-1 mRNA levels remain virtually unchanged. We tested a series of eicosanoids and demonstrate that PGE(2) is a very potent activator of HAS1 in synoviocytes. While mumol concentrations of PGI(2) are required to activate HAS1, low nmol concentrations of PGE(2) are sufficient. In addition, while two thromboxane A(2) analogs moderately activated HAS1 at higher concentrations, the lipoxygenase pathway product LTB(4) was without effect. A series of COX inhibitors blocked IL-1beta induced HAS1 activation. Similarly, sodium salicylate (NaSal) also suppressed IL-1beta induced HAS1 activation. Furthermore, electrophoretic mobility shift assays and PGE(2) ELISA experiments demonstrate that NaSal completely prevents PGE(2) release but does not interfere with NF-kappaB translocation. PGE(2) is a very powerful activator of HAS1 transcription and translation. Such data indicate that the effect of IL-1beta on HAS1 is mediated by prostaglandins. Additionally, NaSal is a potent suppressor of HAS1 activation. These findings point towards HAS1 as a gene of importance in inflammation.
Subject(s)
Dinoprostone/pharmacology , Enzyme Activators/pharmacology , Glucuronosyltransferase/metabolism , Synovial Membrane/drug effects , Base Sequence , Cyclooxygenase 2/genetics , DNA Primers , Electrophoretic Mobility Shift Assay , Hyaluronan Synthases , Interleukin-1beta/physiology , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Synovial Membrane/cytology , Synovial Membrane/enzymologyABSTRACT
Gold compounds are among the oldest disease-modifying drugs and are still widely used today for treating rheumatoid arthritis. Despite decades of use, little is known about the mode of action of this class of drugs. Here we have demonstrated that aurothiomalate (AuTM) suppresses hyaluronan accumulation by blocking interleukin (IL)-1beta-induced hyaluronan synthase-1 transcription. We have further demonstrated that, in fibroblast-like synoviocytes (FLSs), AuTM acts as a specific COX-2 transcriptional repressor in that IL-1beta-induced COX-2 transcription is blocked, whereas COX-1 transcription and translation is unaffected. As a consequence, PGE2 levels released by FLS are dose-dependently reduced in cells exposed to AuTM. Of similar importance is the demonstration that AuTM does block NFkappaB-DNA interaction. In addition, two other transcription factors implicated in inflammatory events, namely AP-1 and STAT3, are blocked as well. The effect on NFkappaB likely explains the inhibition of COX-2 as well as that of HAS1, as both are genes that depend on the activation of NFkappaB. Interestingly, AuTM does not interfere with IL-1beta-induced IkappaB alpha degradation, in most cases a prerequisite for subsequent NFkappaB activation. Furthermore, evidence is presented that, in FLS, AuTM blocks NFkappaB-DNA interaction neither by binding to NFkappaB binding sites nor by interacting with activated NFkappaB proteins. Taken together, AuTM treatment of FLS blocks two of the most important proinflammatory events that are associated with rheumatoid arthritis. AuTM blocks the release of PGE2 and prevents the activation of NFkappaB, therefore blocking IL-1beta-induced hyaluronan accumulation and likely a series of other pro-inflammatory NFkappaB-dependent genes.
Subject(s)
Antirheumatic Agents/pharmacology , Glucuronosyltransferase/genetics , Gold Sodium Thiomalate/pharmacology , Hyaluronic Acid/biosynthesis , Interleukin-1beta/pharmacology , Cell Line , Cyclooxygenase 2/genetics , Cyclooxygenase 2/metabolism , Down-Regulation/drug effects , Fibroblasts/metabolism , Gene Expression Regulation, Enzymologic/drug effects , Glucuronosyltransferase/antagonists & inhibitors , Humans , Hyaluronan Synthases , Hyaluronic Acid/antagonists & inhibitors , Membrane Proteins/genetics , Membrane Proteins/metabolism , NF-kappa B/metabolism , Rheumatic Diseases/genetics , Rheumatic Diseases/metabolism , Synovial Membrane/cytology , Synovial Membrane/metabolism , Transcription, Genetic/drug effectsABSTRACT
HA takes part in a surprisingly large number of biological processes such as embryogenesis, angiogenesis, cell motility, wound healing and cell adhesion. While substantial progress in HA research has indeed been made over the last years, many important questions have not yet been answered. One of the most pertinent questions awaiting an answer is the quest for functional differences of HA synthesized by the three HAS genes. Of similar importance would be investigations into intracellular signaling pathways involved in the activation of this gene family, a field in which to date very little is known. A better understanding of functional differences between the HAS encoding genes not only holds the promise for a better understanding of a series of biological processes but also the opportunity for selective intervention in a number of maladies characterized by abnormalities of HA levels.
Subject(s)
Arthritis, Rheumatoid/metabolism , Atherosclerosis/metabolism , Glucuronosyltransferase/genetics , Hyaluronic Acid/physiology , Animals , Arthritis, Rheumatoid/drug therapy , Arthritis, Rheumatoid/enzymology , Arthritis, Rheumatoid/genetics , Clinical Trials as Topic , Coronary Restenosis/metabolism , Disease Models, Animal , Gene Regulatory Networks , Glucuronosyltransferase/metabolism , Humans , Hyaluronan Synthases , Hyaluronic Acid/administration & dosage , Hyaluronic Acid/biosynthesis , Hyaluronic Acid/blood , Hyaluronic Acid/genetics , Hyaluronic Acid/metabolism , Inflammation/metabolism , Injections, Intra-Articular , Mice , Mice, Knockout , Neoplasms/etiology , Neoplasms/metabolism , RNA, Messenger/metabolism , Research , Signal TransductionABSTRACT
Fibroblast-like synoviocytes (FLS) play a key role in the genesis of rheumatoid arthritis (RA). FLS are among the most versatile cells with the potential to activate an array of genes that are able to initiate and propagate inflammation in RA-affected joints. Controlling activation of FLS might hold the key to restraining inflammation in RA-affected joints. In this study, we investigate the effect and mechanisms of short-term hyperthermia on a series of proinflammatory genes in FLS. In vitro experiments demonstrate that exposure of FLS to elevated temperatures for the duration of 30 min prevents activation of a series of genes with proinflammatory properties. Exposure to hyperthermia reduces IL-1beta-induced prostaglandin E2 release, suppresses activation of the adhesion molecules VCAM-1, ICAM-1, the cytokines TNFalpha, IL-1alpha, IL-1beta, IL-8 as well as COX-2 protein synthesis. Real time reverse transcriptase-polymerase chain reaction showed that hyperthermia altered gene expression at the transcriptional level. The amount and the duration of inhibition is gene-specific and lasts for up to 25 h. As to the mechanism of inhibition, electrophoretic mobility shift assay experiments demonstrated that exposure of FLS to hyperthermia prevents IL-1beta-induced NF-kappaB translocation and subsequent DNA binding. Many mechanisms have been shown to be involved in hyperthermia-mediated effects on NF-kappaB-DNA interactions. We demonstrate by Western blot experiments that in FLS, hyperthermia prevents the phosphorylation and subsequent degradation of IkappaBalpha, therefore retaining the NF-kappaB complex in the cytoplasm. Carefully controlled in vivo tests are certainly needed before one can take full advantage of those phenomena; however, the ease by which the temperature in joints can be modulated might offer an opportunity for manipulating inflammatory processes in joints by simple balneological means.
Subject(s)
Fibroblasts/metabolism , NF-kappa B/genetics , Synovial Membrane/metabolism , Temperature , Arthritis, Rheumatoid/genetics , Arthritis, Rheumatoid/metabolism , Arthritis, Rheumatoid/pathology , Blotting, Western , Cells, Cultured , Cyclooxygenase 2/genetics , Cyclooxygenase 2/metabolism , Dinoprostone/genetics , Dinoprostone/metabolism , Electrophoretic Mobility Shift Assay , Fibroblasts/cytology , Fibroblasts/drug effects , Gene Expression Regulation/drug effects , HSP70 Heat-Shock Proteins/genetics , HSP70 Heat-Shock Proteins/metabolism , Heme Oxygenase-1/genetics , Heme Oxygenase-1/metabolism , Humans , I-kappa B Proteins/metabolism , Intercellular Adhesion Molecule-1/genetics , Intercellular Adhesion Molecule-1/metabolism , Interleukin-1beta/pharmacology , Membrane Proteins/genetics , Membrane Proteins/metabolism , NF-KappaB Inhibitor alpha , NF-kappa B/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Synovial Membrane/cytology , Synovial Membrane/drug effects , Time FactorsABSTRACT
Scientific progress reveals an ever-expanding role of hyaluronan (HA) in diverse biological functions. It has become increasingly clear that HA might also be essential for certain functions of stem cells. CD133+ cells isolated from umbilical cord blood (UCB) seem to represent an alternative to CD34+ cells as a source of transplantable haematopoietic progenitor cells. The aim of this study was to investigate expression patterns of hyaluronan synthases (HAS) genes in freshly isolated and cultured UCB progenitor cells and to compare HAS mRNA levels to those found in non-progenitor cells. CD133+ stem cells were isolated from UCB using an immunomagnetic procedure. Investigation of HAS mRNA expression patterns in CD133+ and CD133- cells by RT-PCR was performed immediately after isolation as well as after cultivation towards myelomonocytic lineage. In addition, activation patterns of mitogen activated protein kinases (MAPK) were analyzed by Western blot experiments. mRNA for HAS1 is undetectable but HAS3 mRNA can be readily detected in freshly isolated CD133+ as well as in CD133- UCB cells. More importantly, our data demonstrate that mRNA for HAS2 can only be detected in CD133+ progenitor cells. In addition, while MAPK are slightly activated in CD133- UCB cells, no significant phosphorylation of MAPK could be observed in CD133+ cells, excluding a role of these kinases in the regulation of HAS2. HAS2 is expressed only in freshly isolated CD133+ cells and quickly diminishes during differentiation. Because of this, HAS2 gene expression might be suitable as a new marker for CD133+ UCB-derived stem cells.
Subject(s)
Fetal Blood/cytology , Gene Expression Regulation, Enzymologic , Glucuronosyltransferase/genetics , Stem Cells/enzymology , AC133 Antigen , Antigens, CD/immunology , Antigens, CD34/immunology , Antigens, Differentiation, Myelomonocytic/immunology , Blotting, Western , Cell Differentiation , Cell Lineage , Fetal Blood/enzymology , Glycoproteins/immunology , Humans , Hyaluronan Synthases , Mitogen-Activated Protein Kinases/metabolism , Peptides/immunology , Proto-Oncogene Proteins c-kit/immunology , RNA, Messenger/genetics , RNA, Messenger/metabolismABSTRACT
BACKGROUND: We evaluated whether cell-free plasma DNA might be an appropriate marker for cell damage during hemodialysis (HD) and whether it correlated with annexin V expression and 7-amino-actinomycin D (7AAD) nuclear staining of blood leukocytes. METHODS: Circulating DNA, annexin V, and 7AAD were measured in HD patients before HD, 20 min after start of HD, and after HD had ended. Healthy volunteers provided control measurements. Necrosis and apoptosis were monitored by gel electrophoresis. RESULTS: Plasma DNA concentrations were not significantly different between controls and patients before HD. Circulating DNA increased significantly (P < 0.05) after 20 min of treatment with HD. Post-HD concentrations of DNA were significantly higher compared with pre-HD and controls (P < 0.005). Agarose gel electrophoresis showed ladders typical of apoptosis in post-HD samples. Two subpopulations of CD45+ leukocytes were defined by flow cytometry: annexin V+/7AAD+ population for apoptosis, and annexin V+/7AAD- for early apoptosis. Compared with healthy controls, mean fluorescence (MF) of 7AAD+ apoptotic cells in the annexin V+/7AAD+ subpopulation before HD was not significantly increased. HD increased MF of 7AAD+ cells in the annexin V+/7AAD+ subpopulation. In this subpopulation, MF of annexin V+ cells was significantly higher (P < 0.01). MF of annexin V+ cells in the annexin V+/7AAD+ subpopulation increased during HD. CONCLUSIONS: During HD, cell-free plasma DNA concentrations, annexin V expression, and 7AAD uptake in leukocytes increases. The increase in plasma DNA, appearing as ladders typical of apoptosis, and the 7AAD uptake in leukocytes demonstrate that the predominant portion of circulating DNA in HD patients originates from apoptotic leukocytes.
Subject(s)
Annexin A5/blood , Apoptosis , DNA/blood , Renal Dialysis , Adult , Aged , Aged, 80 and over , Biomarkers/blood , Cell Nucleus/metabolism , Dactinomycin/analogs & derivatives , Fluorescent Dyes , Humans , Leukocytes/metabolism , Leukocytes/pathology , Middle Aged , NecrosisABSTRACT
OBJECTIVE: Macrophages play an important role in rheumatoid arthritis (RA). RA is a disease characterized by the successive accumulation of leukocytes resulting in subsequent destruction of affected joints. Activation of matrix metalloproteinases (MMP) is essential for many physiological as well as many pathological events owing to the essential role of MMP in cell migration. We analyzed the effectiveness of quinacrine as an inhibitor of MMP activation in leukocytes and investigated the mode of action. METHODS: Leukocytes were isolated and treated with quinacrine with or without phorbol myristic acetate (PMA). ELISA and RT-PCR were used to monitor production of MMP-1, MMP-2, MMP-3, and MMP-8 at the mRNA and protein level. RESULTS: Quinacrine suppressed PMA induced MMP-1 release in mononuclear cells (MNC) in a dose- and time-dependent manner. RT-PCR showed that quinacrine downregulated induced as well as noninduced steady-state mRNA levels of MMP-1, MMP-2, and MMP-8, but had no effect on MMP-3. The observed inhibition was not due to effects of quinacrine on phospholipase A2 (PLA2) activity. Adding exogenous arachidonic acid to reconstitute the blocked PLA2 signaling pathways did not result in restoration of PMA induced mRNA transcription. CONCLUSION: Inhibition of MMP by quinacrine might, in part, account for its reported immunosuppressive action. Synthesizing more potent derivatives of quinacrine may be a means of suppressing undesired MMP activation.
Subject(s)
Antigens, Human Platelet/metabolism , Antirheumatic Agents/pharmacology , Chloroquine/pharmacology , Leukocytes, Mononuclear/drug effects , Matrix Metalloproteinases/metabolism , Quinacrine/pharmacology , Transcription, Genetic/drug effects , Antigens, Human Platelet/genetics , Cell Survival/drug effects , Dose-Response Relationship, Drug , Gene Expression Regulation, Enzymologic , Humans , Leukocytes, Mononuclear/metabolism , Matrix Metalloproteinases/genetics , Phorbol Esters/pharmacology , RNA, Messenger/metabolism , Up-RegulationABSTRACT
It has become increasingly clear that hyaluronan is more than the simple matrix molecule it was once thought to be but instead takes part in a multitude of biological functions. Three genes encode for hyaluronan synthases (HAS). We demonstrated earlier that HAS2 and HAS3 are constitutively activated in type-B synoviocytes (fibroblast-like synoviocytes) and, furthermore, that the only gene that readily responds to stimulation with a series of proinflammatory cytokines is HAS1. Here we probe the involvement of the transcription factor NF-kappaB in induced and noninduced HAS activation. Transforming growth factor (TGF) beta1 as well as interleukin (IL)-1beta are both strong inducers of HAS1 transcription. Stimulation of fibroblast-like synoviocytes with IL-1beta resulted in rapid degradation of IkappaBalpha, an event that was preceded by IkappaBalpha phosphorylation. Interestingly, TGFbeta1 neither affected IkappaBalpha levels, nor did it cause phosphorylation of IkappaBalpha. In addition, TGFbeta1 had no effect on IkappaBbeta and IkappaBepsilon levels. Electrophorectic mobility shift assays demonstrate that IL-1beta is a potent inducer of NF-kappaB translocation; however, TGFbeta1 treatment did not result in shifting bands. Two adenovirus constructs were used to further clarify differences in TGFbeta1- and IL-1beta-induced HAS1 activation. Overexpressing IkappaBalpha completely abolished the IL-1beta effect on HAS1 but did not interfere with TGFbeta1-induced HAS1 mRNA accumulation. Identical results were obtained when a dominant negative IKK was overexpressed. Interestingly, neither overexpression of IkappaBalpha nor of IKK had any effect on HAS2 and HAS3 mRNA levels. Taken together, HAS1 can be activated by distinct pathways; IL-1beta utilizes NF-kappaB, and TGFbeta1 does not. Furthermore, HAS2 and HAS3 are activated without the involvement of NF-kappaB.
Subject(s)
Adenoviridae/genetics , Gene Transfer Techniques , Glucuronosyltransferase/metabolism , I-kappa B Kinase/genetics , I-kappa B Proteins/genetics , Mutation , NF-kappa B/genetics , Blotting, Western , Cells, Cultured , Fibroblasts/metabolism , Genes, Dominant , Glucuronosyltransferase/genetics , Green Fluorescent Proteins/metabolism , Humans , Hyaluronan Synthases , Hyaluronic Acid/chemistry , Interleukin-1/metabolism , NF-KappaB Inhibitor alpha , NF-kappa B/metabolism , Phosphorylation , Protein Transport , RNA/metabolism , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , STAT3 Transcription Factor/metabolism , Signal Transduction , Smad3 Protein/metabolism , Smad4 Protein/metabolism , Synovial Fluid/cytology , Transcription Factors/metabolism , Transcription, Genetic , Transforming Growth Factors/metabolism , Tumor Necrosis Factor-alpha/metabolismABSTRACT
BACKGROUND: FK778, a derivative of the active leflunomide-metabolite, A77 1726, has been shown to be a powerful immunosuppressant in several transplantation models, particularly efficient in the prevention of chronic allograft rejection. However, the cellular and molecular mechanisms underlying these effects of FK778 have not been investigated yet in detail. Because dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) and are essential for the initiation of immune responses including acute and chronic allograft rejection, we investigated whether FK778 affects this particular cell type. METHODS: Allogeneic T cell stimulation by FK778-treated human monocyte-derived DCs was determined by mixed leukocyte cultures. Surface molecule expression was analyzed by flow-cytometric analysis and cytokine production by ELISA from culture supernatants. Activation of NF-kappaB in DCs was assessed by electrophoretic mobility shift assays. RESULTS: Treatment of DCs with FK778 inhibited their potency to stimulate allogeneic T cells. In line, LPS- and CD40L-induced upregulation of DC surface activation markers and production of IL-12 was significantly inhibited, irrespective of whether cells were treated during or after the monocyte to DC differentiation period. The effects of FK778 on DCs were not reversible by exogenous uridine indicating that FK778 acts independently of its action as an inhibitor of pyrimidine synthesis. On the signaling level, activation of NF-kappaB, the essential transcription factor involved in DC maturation and function, was markedly inhibited by FK778. CONCLUSIONS: Inhibition of activation and function of DCs as the central APCs may significantly contribute to the immunosuppressive profile of FK778 when applied after allogeneic organ transplantation.
Subject(s)
Dendritic Cells/drug effects , Dendritic Cells/immunology , Immunosuppressive Agents/pharmacology , Isoxazoles/pharmacology , Alkynes , Antigens, CD/analysis , Cell Differentiation/drug effects , Cells, Cultured , Coculture Techniques , Dendritic Cells/cytology , Humans , Lymphocyte Activation , NF-kappa B/antagonists & inhibitors , Nitriles , T-Lymphocytes/immunology , Uridine/pharmacologyABSTRACT
Despite evidence that points to unfettered hyaluronic acid (HA) production as a culprit in the progression of rheumatic disorders, little is known about differences in regulation and biological functions of the three hyaluronan synthase (HAS) genes. Testing the effects of drugs with proven anti-inflammatory effects could help to clarify biological functions of these genes. In this study, we demonstrate that leflunomide suppresses HA release in fibroblast-like synoviocytes (FLS) in a dose-dependent manner. We further demonstrate that leflunomide suppresses HA synthase activity, as determined by (14)C-glucuronic acid incorporation assays. Additional experiments revealed that in FLS, leflunomide specifically blocked the induction of HAS1. HAS2 and HAS3, genes that are, in contrast to HAS1, constitutively expressed in FLS, are not significantly affected. Leflunomide can function as a NF-kappaB inhibitor. However, EMSA experiments demonstrate that at the concentrations used, leflunomide neither interferes with IL-1beta- nor with PMA-induced NF-kappaB translocation. Furthermore, reconstituting the pyrimidine synthase pathway did not lead to the restoration of IL-1beta-induced HAS1 activation. More importantly, two tyrosine kinase inhibitors mimicked the effect of leflunomide in that both blocked IL-1beta-induced HAS1 activation without affecting HAS2 or HAS3. These data point at HAS1 activation as the possible cause for unfettered HA production in rheumatoid arthritis and might explain, at least in part, the beneficial effects of leflunomide treatment. These findings also support the concept that IL-1beta-induced HAS1 activation depends on the activation of tyrosine kinases, and indicate that leflunomide blocks HA release by suppressing tyrosine kinases rather than through inhibition of NF-kappaB translocation.
Subject(s)
Glucuronosyltransferase/antagonists & inhibitors , Glucuronosyltransferase/genetics , Immunosuppressive Agents/pharmacology , Interleukin-1/physiology , Isoxazoles/pharmacology , NF-kappa B/physiology , Transcription, Genetic/drug effects , Cells, Cultured , Enzyme Activation/drug effects , Enzyme Activation/immunology , Enzyme Inhibitors/pharmacology , Enzyme Repression/drug effects , Fibroblasts/drug effects , Fibroblasts/immunology , Fibroblasts/metabolism , Glucuronosyltransferase/biosynthesis , Glucuronosyltransferase/metabolism , Humans , Hyaluronan Synthases , Hyaluronic Acid/antagonists & inhibitors , Hyaluronic Acid/metabolism , Interleukin-1/antagonists & inhibitors , Leflunomide , NF-kappa B/metabolism , Protein Transport/drug effects , Protein Transport/immunology , Pyrimidines/biosynthesis , Pyrimidines/metabolism , RNA, Messenger/biosynthesis , Synovial Membrane/drug effects , Synovial Membrane/immunology , Synovial Membrane/metabolism , Transcription, Genetic/immunology , Uridine/metabolismABSTRACT
Polyunsaturated fatty acids (PUFAs) modulate immune responses leading to clinically significant beneficial effects in a variety of inflammatory disorders. PUFA effects on T cells have been extensively studied, but their influence on human dendritic cells (DCs), which are the most potent antigen-presenting cells and play a key role in initiating immune responses, has not been elucidated so far. Here we show that PUFAs of the n-3 and n-6 series (arachidonic and eicosapentaenoic acid) affect human monocyte-derived DC differentiation and inhibit their activation by LPS, resulting in altered DC surface molecule expression and diminished cytokine secretion. Furthermore, the potency to stimulate T cells was markedly inhibited in PUFA-treated DCs. The PUFA-mediated block in LPS-induced DC activation is reflected by diminished TNF-alpha, IL-12p40, CD40, and COX-2 mRNA levels. Strikingly, typical LPS-induced signaling events such as degradation of IkappaB and activation of NF-kappaB were not affected by PUFAs, even though DC membrane lipid composition was markedly altered. Arachidonic and eicosapentaenoic acid both altered DC prostaglandin production, but inhibitors of cyclooxygenases and lipoxygenases did not abolish PUFA effects, indicating that the observed PUFA actions on DCs were independent of autoregulation via eicosanoids. These data demonstrate a unique interference with DC activation and function that could significantly contribute to the well known anti-inflammatory effects of PUFAs.