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1.
Vet Microbiol ; 244: 108690, 2020 May.
Article in English | MEDLINE | ID: mdl-32402349

ABSTRACT

The in vitro investigation of cytokine secretion induced by porcine reproductive and respiratory syndrome virus (PRRSV) requires porcine alveolar macrophages (PAMs) and their interaction with immunocytes. However, immortalized monoclonal PAMs (mPAMs) are non-permissive for PRRSV infection. The porcine CD163 receptor isolated from primary PAMs (pPAMs) confers susceptibility to PRRSV infection; thus, this approach could be used to establish a novel cell line to facilitate the exploration of PRRSV infection kinetics. Here, we amplified the coding region of the CD163 gene from pPAMs and integrated it into an mPAM line using a lentivirus expression system. After verification, the monoclonal PAM cell line stably expressing CD163 (mPAM-CD163-GFP) was infected with either the highly pathogenic PRRSV strain JXA1 or the classical PRRSV strain SD1, which produced high infectious titers of progeny virus reaching > 109 copies/mL or a 50 % tissue culture infective dose of 105.5 over at least 100 cell generations. We also investigated cytokine and Toll-like receptor expression in infected mPAM-CD163-GFP cells and pPAMs. The mPAM-CD163-GFP cell line showed similar patterns of viral replication and cytokine secretion compared with pPAMs, so it may be extremely useful for replacing primary cells for in vitro investigations of the mechanisms of cytokine secretion and interactions between PRRSV-infected PAMs and immunocytes.


Subject(s)
Antigens, CD/genetics , Antigens, Differentiation, Myelomonocytic/genetics , Cytokines/immunology , Macrophages, Alveolar/immunology , Macrophages, Alveolar/virology , Porcine respiratory and reproductive syndrome virus/physiology , Receptors, Cell Surface/genetics , Virus Replication , Animals , Cell Line , Cells, Cultured , Lentivirus/genetics , Swine , Virus Cultivation
2.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 35(3): 310-3, 2015 Mar.
Article in Chinese | MEDLINE | ID: mdl-25951636

ABSTRACT

OBJECTIVE: To observe the clinical efficacy of comprehensive Western medical treatment plus Compound Yindan Decoction (CYD) in treatment of acute cholestatic hepatitis (ACH). METHODS: Using randomized controlled study, 60 ACH patients in line with inclusive criteria were randomly assigned to the treatment group (treated by comprehensive Western medical treatment plus CYD) and the control group (treated by comprehensive Western medical treatment alone), 30 in each group. Scores for symptoms and levels of liver functions [total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), total biliary acid (TBA)] were observed before and after treatment. RESULTS: Compared with before treatment in the same group, total scores for symptoms decreased in the treatment group and the control group at the end of the 1st and the 4th week after treatment (all P < 0.05). Compared with the control group, total scores for symptoms decreased in the treatment group at the end of the 1st week (P < 0.05). Compared with before treatment, serum levels of TBIL, DBIL, ALP, GGT, and TBA all decreased in the two groups at the end of the 4th week after treat- ment (P < 0.01). Compared with the control group, serum levels of TBIL, DBIL, ALP, GGT, and TBA all decreased in the treatment group at the end of the 1st and the 2nd week after treatment (P < 0.05). Compared with the control group, the average time for TBIL and DBIL decreasing to the level less than five times the normal value was significantly shorter in the treatment group (P < 0.05). CONCLUSION: CYD could significantly improve clinical symptoms of ACH patients, decrease serum levels of TBIL and DBIL, reduce serum levels of ALP, GGT, and TBA, obviously improve cholestasis, and promote the recovery.


Subject(s)
Drugs, Chinese Herbal/therapeutic use , Hepatitis/drug therapy , Alkaline Phosphatase , Bilirubin , Cholestasis , Combined Modality Therapy , Humans , gamma-Glutamyltransferase
3.
Huan Jing Ke Xue ; 36(9): 3339-44, 2015 Sep.
Article in Chinese | MEDLINE | ID: mdl-26717696

ABSTRACT

The treatment of decentralized sewage has gained more and more attention in China in recent years. A four-zone integrated reactor was designed by combining biofilm system and activated sludge system and start-up by gradient shorten the HRT. The removal of COD, NH4+ -N and TN were studied at 8-15°C. Fluorescence in situ hybridization(FISH) was used to detect nitrobacteria population (AOB,NOB) so as to study the relationship between the reactor effect and functional micro-bacteria. The results showed that, when the HRT was 9.2 h, the removal efficiencies of COD, ammonia and TN were 92. 11%, 99. 21% and 61. 63%, respectively. Compared to the initial stage, the numbers of AOB and NOB in the late phase were increased by 5. 82 and 6. 14 times, respectively. In addition, the proportion of nitrobacteria was increased from 6. 12% to 16. 38% , which became the dominant bacteria in biofilms. Moreover, the nitrification efficiency was increased from 78. 49% to 97. 52% , while the number of NOB was 5. 61-fold increased and the value of AOB/NOB was optimized to 1. 47. The effluent quality is guaranteed by the enrichment of AOB and NOB and suitable value of AOB/NOB.


Subject(s)
Bioreactors/microbiology , Denitrification , Nitrobacter/classification , Nitrogen/chemistry , Ammonia , Biofilms , China , In Situ Hybridization, Fluorescence , Nitrification , Seasons , Sewage/microbiology
4.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 34(4): 412-7, 2014 Apr.
Article in Chinese | MEDLINE | ID: mdl-24812894

ABSTRACT

OBJECTIVE: To evaluate the clinical efficacy and safety of integrative medical program based on blood cooling and detoxification recipe (BCDR) in treating patients with hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) of heat-toxicity accumulation syndrome (HTAS). METHODS: Adopting randomized controlled clinical design, a total of 105 HBV-ACLF patients of HTAS were randomly assigned to the trial group (64 cases) and the control group (41 cases). Patients in the control group were treated with comprehensive Western therapy, while those in the trial group were treated with comprehensive Western therapy plus BCDR. All were treated for 8 weeks and followed up for 40 weeks. Effect and safety of the treatment were assessed, including fatality, liver functions [total bilirubin (TBIL), albumin (ALB), alanine aminotransferase (ALT), and aspartate transaminase (AST)], and prothrombin activity (PTA) after treatment and at week 48 of follow-ups. RESULTS: After 8-week treatment, there was statistical difference in the overall fatality rate (15.63% vs 34.15%), the fatality rate in the mid-term (25.0% vs 64.7%), TBIL at week 8 (64.54 +/- 79.75), AST [at week 2: (178.97 +/- 44.24) U/L vs (288.48 +/- 58.49) U/L; at week 4: (61.65 +/- 27.36) U/L vs (171.12 +/- 89.11) U/L] and PTA [at week 4: (58.30 +/- 15.29) vs (42.56 +/- 15.27); at week 6: (60.77 +/- 20.40) vs (43.08 +/- 12.79)] (all P < 0.05). At week 48 of the followup, the fatality rate of the trial group (21.88%) decreased by 17. 14% when compared with that of the control group (39.02%; P < 0.05). No obvious adverse event occurred in the two groups during the 8-week treatment period. CONCLUSION: BCDR could significantly reduce the mortality of HBV-ACLF patients.


Subject(s)
Acute-On-Chronic Liver Failure/drug therapy , Drugs, Chinese Herbal/therapeutic use , Hepatitis B, Chronic/drug therapy , Phytotherapy , Acute-On-Chronic Liver Failure/virology , Adult , End Stage Liver Disease , Female , Hepatitis B virus , Humans , Male , Middle Aged , Young Adult
5.
Ying Yong Sheng Tai Xue Bao ; 21(11): 2792-8, 2010 Nov.
Article in Chinese | MEDLINE | ID: mdl-21361001

ABSTRACT

In order to explore the effects of long-term fertilization on the microbiological characters of red soil, soil samples were collected from a 19-year long-term experimental field in Qiyang of Hunan, with their microbial biomass carbon (MBC) and nitrogen (MBN) and microbial utilization ratio of carbon sources analyzed. The results showed that after 19-year fertilization, the soil MBC and MBN under the application of organic manure and of organic manure plus inorganic fertilizers were 231 and 81 mg x kg(-1) soil, and 148 and 73 mg x kg(-1) soil, respectively, being significantly higher than those under non-fertilization, inorganic fertilization, and inorganic fertilization plus straw incorporation. The ratio of soil MBN to total N under the application of organic manure and of organic manure plus inorganic fertilizers was averagely 6.0%, significantly higher than that under non-fertilization and inorganic fertilization. Biolog-ECO analysis showed that the average well color development (AWCD) value was in the order of applying organic manure plus inorganic fertilizers = applying organic manure > non-fertilization > inorganic fertilization = inorganic fertilization plus straw incorporation. Under the application of organic manure or of organic manure plus inorganic fertilizers, the microbial utilization rate of carbon sources, including carbohydrates, carboxylic acids, amino acids, polymers, phenols, and amines increased; while under inorganic fertilization plus straw incorporation, the utilization rate of polymers was the highest, and that of carbohydrates was the lowest. Our results suggested that long-term application of organic manure could increase the red soil MBC, MBN, and microbial utilization rate of carbon sources, improve soil fertility, and maintain a better crop productivity.


Subject(s)
Carbon/analysis , Fertilizers , Nitrogen/analysis , Soil Microbiology , Soil/analysis , Agriculture/methods , Time Factors , Triticum/growth & development
6.
Acta Crystallogr Sect E Struct Rep Online ; 65(Pt 12): o3198, 2009 Nov 25.
Article in English | MEDLINE | ID: mdl-21578908

ABSTRACT

There are two mol-ecules in the asymmetric unit of the title compound, C(17)H(20)O(4)S(2). There are slight differences in the twist of the two rings relative to the S-C-S chain [dihedral angles of 48.41 (18) and 87.58 (16)° in the first mol-ecule and 45.98 (18) and 87.02 (18)° in the second] and the difference in the C-S-C-S torsion angles [176.68 (17) and -77.6 (2)° for the two independent mol-ecules].

7.
Acta Crystallogr Sect E Struct Rep Online ; 65(Pt 12): o3199, 2009 Nov 25.
Article in English | MEDLINE | ID: mdl-21578909

ABSTRACT

In the title compound, C(23)H(22)N(2)O(6), the crystal packing is stabilized by inter-molecular N-H⋯O hydrogen bonds, which link the mol-ecules into chains running parallel to the c axis. Inter-molecular C-H⋯O hydrogen bonds are also present in the structure.

8.
Zhonghua Gan Zang Bing Za Zhi ; 14(9): 655-7, 2006 Sep.
Article in Chinese | MEDLINE | ID: mdl-16995977

ABSTRACT

OBJECTIVE: To evaluate the relationship between mortality and HBVDNA and HBeAg expression of severe hepatitis B patients. METHODS: The mortality rates of different types of severe hepatitis patients in our hospital during the last five years were analysed. HBV DNA was detected using the fluorescence quantitative PCR method and the HBeAg expression of severe hepatitis B was studied using a microparticle method. RESULTS: (1) Hepatitis B morbidity was 83.5% in each type of severe hepatitis, and severe chronic hepatitis B morbidity was 96.77% in each type of severe chronic hepatitis. (2) The mortality rate of those with HBV DNA more than 1 x 10(5) copies/ml was 53.25% and the mortality of those with HBV DNA less than 1 x 10(5) copies/ml was 34.50% (P less than 0.01). The HBeAg expression had no influence on the death rate. (3) The death rate descended to 30.38% from 54.64% (HBV DNA more than 1 x 10(5) copies/ml) when treated with Lamivudine (P less than 0.01). CONCLUSION: In severe hepatitis the quantity of virus carried in the patient is one of the key factors of mortality; antivirus treatment can lower mortality.


Subject(s)
Hepatitis B virus , Hepatitis B, Chronic/diagnosis , Hepatitis B, Chronic/virology , Adolescent , Adult , Aged , Aged, 80 and over , Child , DNA, Viral , Female , Hepatitis B Surface Antigens , Hepatitis B virus/genetics , Hepatitis B virus/immunology , Hepatitis B virus/physiology , Humans , Male , Middle Aged , Viral Load , Young Adult
9.
Liver Int ; 23 Suppl 3: 16-20, 2003.
Article in English | MEDLINE | ID: mdl-12950956

ABSTRACT

BACKGROUND: Molecular Adsorbents Recirculating System (MARS) is a new promising artificial liver support therapy, the aim of this study was to assess the effectiveness of MARS to remove nitrous oxide (NO) and cytokines in severe liver failure patients with multiple organ dysfunction syndrome (MODS). METHODS: Sixty single MARS treatments were performed with length of 6-24 h on 24 severe liver failure patients (18 males/6 females) with MODS. RESULTS: The MARS therapy was associated with a significant removal of NO and certain cytokines such as TNF-alpha, IL-6, IL-8, and INF-gamma, together with marked reduction of other non-water-soluble albumin bound toxins and water-soluble toxins, these were associated with a improvement of the patients' clinical conditions including hepatic encephalopathy, deranged hemodynamic situation and as well as renal and respiratory function, thus resulted into marked decrease of Sequential Organ Failure Assessment (SOFA) score and improved outcome: nine patients were able to be discharged from the hospital or bridged to successful liver transplantation, the overall survival of 24 patients was 37.5%. CONCLUSION: We can confirm the positive therapeutic impact and safety to use MARS on liver failure patients with MODS associated with elevated levels of NO and cytokines.


Subject(s)
Cytokines/blood , Liver Failure, Acute/therapy , Multiple Organ Failure/therapy , Nitrous Oxide/blood , Renal Dialysis , Sorption Detoxification , Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Interferon-gamma/blood , Interleukin-6/blood , Interleukin-8/blood , Liver Failure, Acute/blood , Male , Middle Aged , Multiple Organ Failure/blood , Severity of Illness Index , Toxins, Biological/blood , Tumor Necrosis Factor-alpha/metabolism
10.
Zhonghua Gan Zang Bing Za Zhi ; 11(8): 455-7, 2003 Aug.
Article in Chinese | MEDLINE | ID: mdl-12939172

ABSTRACT

OBJECTIVE: To evaluate the effectiveness and mechanisms of molecular adsorbents recirculating system (MARS) treatment in severe liver failure patients with multiple organ dysfunction syndrome (MODS). METHODS: 60 single MARS treatments were performed for 6 - 24 hours on 24 severe liver failure patients with MODS. RESULTS: MARS therapy was associated with marked reduction of albumin bound toxins and water soluble toxins, together with a significant removal of NO and certain cytokines, such as TNF-alpha, IL-6, IL-8, and INF-gamma. These were associated with a improvement of the patients' clinical conditions including hepatic encephalopathy, deranged hemodynamic situation, as well as renal and respiratory function, thus resulted into marked decrease of sequential organ failure assessment (SOFA) score (from 9.72+-1.89 to 6.98+-2.34), and improving outcome: 9 patients were able to be discharged from the hospital or bridged to successful liver transplantation. The overall survival rate of 24 patients was 37.5%. CONCLUSIONS: There is positive therapeutic impact and safety to use MARS on liver failure patients with MODS. The effectiveness of MARS is correlated with reducing the levels of NO and cytokines, except for completely removing of accumulated toxins in liver failure patients.


Subject(s)
Liver Failure, Acute/therapy , Liver, Artificial , Multiple Organ Failure/therapy , Sorption Detoxification , Adolescent , Adult , Aged , Aged, 80 and over , Bioreactors , Female , Humans , Interferon-gamma/blood , Interleukin-6/blood , Interleukin-8/blood , Liver Failure, Acute/blood , Male , Middle Aged , Nitric Oxide/blood , Sorption Detoxification/instrumentation , Sorption Detoxification/methods , Tumor Necrosis Factor-alpha/metabolism
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